FastQCFastQC Report
Thu 26 May 2016
SRR1512842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589366
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT132200.8317782059009694No Hit
TATCAACGCAGAGTACTTTTTTTTT98800.6216315184796957No Hit
GGTATCAACGCAGAGTACTTTTTTT78180.49189425217350824No Hit
GTCCTACAGTGGACATTTCTAAATT55930.35190132417580344No Hit
CTGTAGGACGTGGAATATGGCAAGA51680.32516110197399467No Hit
GTCCTAAAGTGTGTATTTCTCATTT50870.3200647302131794No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48320.30402059689209404No Hit
CTTTAGGACGTGAAATATGGCGAGG48080.3025105608148155No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29300.184350237767764No Hit
CTGAAGGACCTGGAATATGGCGAGA25140.1581762790949347No Hit
GTCCTACAGTGTGCATTTCTCATTT24010.1510665258977479No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23820.14987108066990235No Hit
ATTTAGAAATGTCCACTGTAGGACG18260.1148885782129478No Hit
GTACTGGTTCACTATCGGTCAGTCA18250.1148256600430612No Hit
TTTCTAAATTTTCCACCTTTTTCAG17710.11142807886918432No Hit
GTCCTTCAGTGTGCATTTCTCATTT17500.11010679730156553No Hit
CTGTAGGACCTGGAATATGGCGAGA16990.10689797063734847No Hit
GAATATGGCAAGAAAACTGAAAATC16540.10406665299245108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATG852.7261012E-713.40081718
GGTATCA19350.013.3729751
AAGGCCG604.0595603E-412.6786635
TAGGACC7850.012.5979084
GGCGAGG12200.012.52625719
TATACTG2550.011.9328595
TAGGACA7100.011.6518714
CGAATGG1001.9492636E-611.39033519
CCACCTT5800.011.1312913
CTGCGCA600.005889466511.0798649
GCGCCAC951.3691566E-510.99344113
CACCTTT5800.010.96759514
GTAGGCC700.001477366510.8694823
CCGTACA2550.010.79510217
TGTAGGA25300.010.7137432
AGGACCT16700.010.7047415
CCCGCCC2850.010.66030714
GTCCTAC27300.010.6286311
GTCTAGT909.3784896E-510.570551
CTACACT1909.094947E-1210.5099454
Produced by FastQC (version 0.11.5)