##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512842_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1589366 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.244897651013044 31.0 30.0 34.0 26.0 34.0 2 30.272475943237744 31.0 30.0 34.0 26.0 34.0 3 30.427425149399195 31.0 30.0 34.0 26.0 34.0 4 33.97967806030832 37.0 35.0 37.0 30.0 37.0 5 33.612098786560175 37.0 35.0 37.0 28.0 37.0 6 33.449900778046086 37.0 35.0 37.0 28.0 37.0 7 33.26678939904339 37.0 35.0 37.0 27.0 37.0 8 33.20322820546054 37.0 35.0 37.0 26.0 37.0 9 34.46413915989143 39.0 35.0 39.0 26.0 39.0 10 34.40759019634244 39.0 35.0 39.0 26.0 39.0 11 34.37010921335929 39.0 35.0 39.0 26.0 39.0 12 34.25711950551352 39.0 35.0 39.0 25.0 39.0 13 34.243466891829826 39.0 35.0 39.0 25.0 39.0 14 35.301422076475774 40.0 35.0 41.0 25.0 41.0 15 35.315038826802635 40.0 36.0 41.0 25.0 41.0 16 35.33042106097651 40.0 36.0 41.0 25.0 41.0 17 35.36051607999668 40.0 36.0 41.0 25.0 41.0 18 35.30334485574751 40.0 36.0 41.0 25.0 41.0 19 35.33991100854051 40.0 36.0 41.0 25.0 41.0 20 35.33082562480889 40.0 36.0 41.0 25.0 41.0 21 35.29807482983781 40.0 36.0 41.0 25.0 41.0 22 35.264422417492256 40.0 36.0 41.0 25.0 41.0 23 35.12478686469951 40.0 35.0 41.0 25.0 41.0 24 35.091681840431974 40.0 35.0 41.0 24.0 41.0 25 35.0366775179537 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6976683757196902 1101 2 0.4799785373713341 1101 3 0.516094581663257 1101 4 0.6198881911649821 1101 5 0.7022791924451255 1101 6 0.762200886652046 1101 7 0.8537503542625657 1101 8 0.5210641931950377 1101 9 0.8740749604657196 1101 10 0.5981577158769582 1101 11 0.821380304820579 1101 12 0.9708470165657275 1101 13 1.0172109509434648 1101 14 1.0849898800571722 1101 15 0.7928125012918557 1101 16 0.9626455812659955 1101 17 1.1124342475760116 1101 18 0.9134493473018637 1101 19 0.8121797658981862 1101 20 0.8861380436096695 1101 21 0.9457577444166745 1101 22 0.8930048361083323 1101 23 1.0659342118535164 1101 24 0.9959618646273327 1101 25 1.0887784359572166 1102 1 0.7527100162394973 1102 2 0.8232596085149737 1102 3 0.5616738437068456 1102 4 0.5535069588830908 1102 5 0.8122087457043108 1102 6 0.7176685502105258 1102 7 0.7319829735632126 1102 8 0.8346879432699268 1102 9 0.9526241801592761 1102 10 0.8810784806371927 1102 11 1.037212531839458 1102 12 0.9693229830010424 1102 13 1.2184776678541525 1102 14 1.1366391032194585 1102 15 1.2319389806261114 1102 16 1.1982596567392392 1102 17 1.1521314905668163 1102 18 0.5791391987456436 1102 19 1.0074708080621662 1102 20 1.0929563042626143 1102 21 0.9754997340167932 1102 22 0.758666622377838 1102 23 1.1570921052626204 1102 24 1.139106709307086 1102 25 1.0704657520751368 1103 1 0.909269542136979 1103 2 1.0556749220755712 1103 3 0.736273450467607 1103 4 0.6665932407414701 1103 5 0.8726646632163835 1103 6 0.8488539911277115 1103 7 0.9187200725161091 1103 8 0.9652281395203062 1103 9 1.1112763665140548 1103 10 0.9218521280696024 1103 11 1.0658731491450624 1103 12 1.2327043481381352 1103 13 1.2607970408868 1103 14 1.3296504911570395 1103 15 1.3288289136400522 1103 16 1.2616859459208527 1103 17 1.1598139637277782 1103 18 1.2116657454712723 1103 19 1.1151454349002847 1103 20 1.0324319237374908 1103 21 1.1438072052546389 1103 22 1.0316983492150342 1103 23 1.1570357963575049 1103 24 1.179222496416351 1103 25 1.1436337895405586 1104 1 0.5373519772031692 1104 2 0.6786261767049382 1104 3 0.549639702370115 1104 4 0.4585119651254459 1104 5 0.587298672650256 1104 6 0.5924852970182712 1104 7 0.5154846237690194 1104 8 0.4803009916388845 1104 9 0.6496606082980634 1104 10 0.6224590910279275 1104 11 0.7052646709264678 1104 12 0.6532844658376646 1104 13 0.5531542398760578 1104 14 0.7810729033900401 1104 15 0.8878203883072899 1104 16 0.8071058723908564 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1107 16 0.515144150273116 1107 17 0.38854381189354825 1107 18 0.4447288242745344 1107 19 0.4557537232097175 1107 20 0.5847523382907838 1107 21 0.4904945533503877 1107 22 0.45434860410964717 1107 23 0.5411813381358144 1107 24 0.5827659916578867 1107 25 0.6469180556054539 1108 1 0.5366515003758039 1108 2 0.458770638445845 1108 3 0.4889581644715939 1108 4 0.2788109091094597 1108 5 0.2944321262136427 1108 6 0.4964590280890775 1108 7 0.2980521543471255 1108 8 0.29185056377601626 1108 9 0.39830113221520236 1108 10 0.3733076592580815 1108 11 0.40486537817147905 1108 12 0.5920548849318408 1108 13 0.5676396454608224 1108 14 0.6672764148183816 1108 15 0.4842783534086337 1108 16 0.40083275650581385 1108 17 0.5178098245560676 1108 18 0.7391920126322731 1108 19 0.7410535903746194 1108 20 0.6662723734433982 1108 21 0.6285571112875985 1108 22 0.7307894221747517 1108 23 0.7508857225331766 1108 24 0.6739198629089813 1108 25 0.7319488669528482 1109 1 0.4293926165926507 1109 2 0.45086563842582805 1109 3 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-2.6722959974473497 2216 18 -3.066083390046245 2216 19 -3.0834262792586955 2216 20 -2.429853049339094 2216 21 -3.0364238021118055 2216 22 -2.5891469155732807 2216 23 -3.75917590138771 2216 24 -2.989640887016634 2216 25 -3.104788563383895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44027.0 3 0.0 4 662.0 5 6239.0 6 8734.0 7 6912.0 8 5526.0 9 4532.0 10 3705.0 11 3233.0 12 2836.0 13 2593.0 14 2525.0 15 2502.0 16 2538.0 17 2722.0 18 3037.0 19 3790.0 20 5317.0 21 7234.0 22 9056.0 23 10653.0 24 12473.0 25 13900.0 26 15474.0 27 16638.0 28 18532.0 29 22237.0 30 27147.0 31 35032.0 32 43995.0 33 56985.0 34 73804.0 35 97067.0 36 133255.0 37 209416.0 38 675038.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.583175903395954 20.620802917869433 14.37562027395328 25.42040090478134 2 12.499669537390114 21.47711121546036 42.357375484835856 23.665843762313663 3 16.82855350257666 26.730993478587834 30.52433714498956 25.916115873845946 4 10.743877055348753 17.95367427016715 38.7191461729551 32.583302501529005 5 11.32497699889368 40.32462081213099 35.67732751687482 12.673074672100507 6 29.7956074328004 38.60416214157325 18.636270630491104 12.96395979513525 7 26.614973575191854 33.36017898218679 22.397960822144324 17.626886620477038 8 24.396363068601058 38.41253812079286 20.713472662575267 16.477626148030815 9 26.555859123496344 14.876129804560566 21.19837797796059 37.36963309398249 10 15.126820660710116 30.297519110760256 34.135724011852666 20.43993621667696 11 32.396393017842996 23.322810556671037 25.985133877530984 18.29566254795498 12 23.43109212925503 26.30584501627705 31.994760135249916 18.268302719217996 13 28.558493424873543 23.507925451487402 26.590391550212146 21.343189573426912 14 19.727886876242263 24.59645173244978 28.93523659835944 26.740424792948513 15 21.783440532579192 33.94588247287611 24.159003509540327 20.111673485004378 16 19.73889629663656 29.378791663861232 30.30423923829756 20.578072801204648 17 19.429657651127318 30.67856909144908 29.417350005285492 20.47442325213811 18 19.312822674775287 27.611553841265245 33.568244240225766 19.5073792437337 19 21.987426084833658 27.421944703464746 30.244217345509593 20.346411866192 20 22.708645444472477 27.64053102256261 30.938563943746693 18.71225958921822 21 21.28651011668892 27.194004608161553 29.657898743766335 21.86158653138319 22 21.841745467174967 29.62347524241027 29.618252985023446 18.916526305391322 23 19.8375327017188 29.234801801473044 31.02671127984366 19.9009542169645 24 21.773461870959867 27.219910329024277 31.640918454276733 19.36570934573912 25 20.577337490129704 28.463631701969028 31.227565725934568 19.731465081966697 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 158.0 1 158.0 2 560.5 3 963.0 4 963.0 5 963.0 6 2424.0 7 3885.0 8 3885.0 9 3885.0 10 4719.0 11 5553.0 12 5553.0 13 5553.0 14 7590.0 15 9627.0 16 9627.0 17 9627.0 18 17027.5 19 24428.0 20 24428.0 21 24428.0 22 39406.5 23 54385.0 24 54385.0 25 54385.0 26 79537.0 27 104689.0 28 104689.0 29 104689.0 30 131026.5 31 157364.0 32 157364.0 33 157364.0 34 170397.5 35 183431.0 36 183431.0 37 183431.0 38 187486.0 39 191541.0 40 191541.0 41 191541.0 42 196128.5 43 200716.0 44 200716.0 45 200716.0 46 204871.0 47 209026.0 48 209026.0 49 209026.0 50 191495.5 51 173965.0 52 173965.0 53 173965.0 54 144782.5 55 115600.0 56 115600.0 57 115600.0 58 94658.0 59 73716.0 60 73716.0 61 73716.0 62 59135.5 63 44555.0 64 44555.0 65 44555.0 66 32676.5 67 20798.0 68 20798.0 69 20798.0 70 15147.0 71 9496.0 72 9496.0 73 9496.0 74 6565.0 75 3634.0 76 3634.0 77 3634.0 78 2478.0 79 1322.0 80 1322.0 81 1322.0 82 864.0 83 406.0 84 406.0 85 406.0 86 246.0 87 86.0 88 86.0 89 86.0 90 51.5 91 17.0 92 17.0 93 17.0 94 11.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08544287470601485 2 0.04303602820244047 3 0.018435023776776402 4 0.00616598064888767 5 0.01963046900462197 6 0.06298108805649548 7 0.08022066660542632 8 0.06254066086728922 9 0.030515312395005302 10 0.05429838061214346 11 0.0072355895369600205 12 7.550180386393065E-4 13 0.007487262216506456 14 0.0016987905869384396 15 0.016924987699497785 16 0.011891534108569078 17 0.006920998687526976 18 0.006983916857413584 19 0.00276839947501079 20 0.003460499343763488 21 7.550180386393065E-4 22 9.437725482991331E-4 23 0.0 24 0.0 25 6.291816988660887E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1589366.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.07549116457078 #Duplication Level Percentage of deduplicated Percentage of total 1 78.02400821919201 45.31282599958084 2 13.502163138711815 15.682895121297024 3 4.162139385487206 7.251548673227229 4 1.7412962605469742 4.045065423771837 5 0.8361576683773329 2.4280133641017954 6 0.459044036245009 1.599552472265756 7 0.2786397651738891 1.1327498854317992 8 0.18399888228281075 0.8548660369845019 9 0.11994286545482814 0.6269166740677656 >10 0.5946934067218865 6.1832093794143965 >50 0.04935483279848327 2.0182394114758906 >100 0.039960065023601894 4.80200485423471 >500 0.005117332623540799 2.088308503271686 >1k 0.002830864855575761 3.0166844932503363 >5k 5.443970876107233E-4 2.121189276653303 >10k+ 1.0887941752214466E-4 0.8359304309711043 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 13220 0.8317782059009694 No Hit TATCAACGCAGAGTACTTTTTTTTT 9880 0.6216315184796957 No Hit GGTATCAACGCAGAGTACTTTTTTT 7818 0.49189425217350824 No Hit GTCCTACAGTGGACATTTCTAAATT 5593 0.35190132417580344 No Hit CTGTAGGACGTGGAATATGGCAAGA 5168 0.32516110197399467 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5087 0.3200647302131794 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4832 0.30402059689209404 No Hit CTTTAGGACGTGAAATATGGCGAGG 4808 0.3025105608148155 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2930 0.184350237767764 No Hit CTGAAGGACCTGGAATATGGCGAGA 2514 0.1581762790949347 No Hit GTCCTACAGTGTGCATTTCTCATTT 2401 0.1510665258977479 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2382 0.14987108066990235 No Hit ATTTAGAAATGTCCACTGTAGGACG 1826 0.1148885782129478 No Hit GTACTGGTTCACTATCGGTCAGTCA 1825 0.1148256600430612 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1771 0.11142807886918432 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1750 0.11010679730156553 No Hit CTGTAGGACCTGGAATATGGCGAGA 1699 0.10689797063734847 No Hit GAATATGGCAAGAAAACTGAAAATC 1654 0.10406665299245108 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.291816988660887E-5 2 0.0 0.0 0.0 0.0 6.291816988660887E-5 3 0.0 0.0 0.0 0.0 6.291816988660887E-5 4 0.0 0.0 0.0 0.0 6.291816988660887E-5 5 0.0 0.0 0.0 0.0 6.291816988660887E-5 6 0.0 0.0 0.0 0.0 6.291816988660887E-5 7 0.0 0.0 0.0 0.0 6.291816988660887E-5 8 0.0 0.0 0.0 0.0 6.291816988660887E-5 9 0.0 0.0 0.0 0.0 6.291816988660887E-5 10 0.0 0.0 0.0 0.0 6.291816988660887E-5 11 0.0 0.0 0.0 0.0 6.291816988660887E-5 12 0.0 0.0 0.0 0.0 6.291816988660887E-5 13 0.0 0.0 0.0 0.0 6.291816988660887E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAATG 85 2.7261012E-7 13.400817 18 GGTATCA 1935 0.0 13.372975 1 AAGGCCG 60 4.0595603E-4 12.678663 5 TAGGACC 785 0.0 12.597908 4 GGCGAGG 1220 0.0 12.526257 19 TATACTG 255 0.0 11.932859 5 TAGGACA 710 0.0 11.651871 4 CGAATGG 100 1.9492636E-6 11.390335 19 CCACCTT 580 0.0 11.13129 13 CTGCGCA 60 0.0058894665 11.079864 9 GCGCCAC 95 1.3691566E-5 10.993441 13 CACCTTT 580 0.0 10.967595 14 GTAGGCC 70 0.0014773665 10.869482 3 CCGTACA 255 0.0 10.795102 17 TGTAGGA 2530 0.0 10.713743 2 AGGACCT 1670 0.0 10.704741 5 CCCGCCC 285 0.0 10.660307 14 GTCCTAC 2730 0.0 10.628631 1 GTCTAGT 90 9.3784896E-5 10.57055 1 CTACACT 190 9.094947E-12 10.509945 4 >>END_MODULE