FastQCFastQC Report
Thu 26 May 2016
SRR1512841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748894
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT89480.5116376407032102No Hit
GTCCTAAAGTGTGTATTTCTCATTT78910.4511994437627438No Hit
CTGTAGGACGTGGAATATGGCAAGA78560.4491981789633906No Hit
CTTTAGGACGTGAAATATGGCGAGG60940.3484487910645242No Hit
GTATCAACGCAGAGTACTTTTTTTT48580.277775554150223No Hit
GTCCTACAGTGTGCATTTCTCATTT41390.2366638572720817No Hit
CTGAAGGACCTGGAATATGGCGAGA32610.1864607003054502No Hit
GGTATCAACGCAGAGTACTTTTTTT28490.16290295466734978No Hit
TATCAACGCAGAGTACTTTTTTTTT28160.16101604785653104No Hit
GTCCTTCAGTGTGCATTTCTCATTT27780.15884324607437617No Hit
ATTTAGAAATGTCCACTGTAGGACG25360.1450059294617055No Hit
CTGTAGGACCTGGAATATGGCGAGA25120.14363363359929188No Hit
TTTCTAAATTTTCCACCTTTTTCAG24080.13768701819549956No Hit
GTACTGGTTCACTATCGGTCAGTCA23190.1325980877057157No Hit
GAATATGGCAAGAAAACTGAAAATC21910.12527917643950975No Hit
GCCATATTCCACGTCCTACAGTGGA20100.11492977847714042No Hit
ATTCCAGGTCCTTCAGTGTGCATTT19140.10944059502748593No Hit
GGAATATGGCGAGAAAACTGAAAAT18980.10852573111921018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10500.013.2077534
GGTATCA14950.012.0377081
TATGTCG1603.6379788E-1211.87374816
GGCGAGG16200.011.66852319
AGGACTG2650.011.470165
TAGGACT4150.011.4442494
GTACTTA1604.3655746E-1111.3072451
GTCCTAC38350.011.2971581
ATAGGAC3200.011.2797373
TCCCGTT1354.7384674E-911.257750511
ACTATAC855.3307776E-511.1749733
GTCCTAA21200.011.1388151
TAGAAAT7850.011.1322654
AGCTTCG600.005880768411.08216519
ACTGTTC3950.011.0618098
GTCCTAG3300.010.96460151
GCCGGTT1303.272544E-810.96006911
CTCTAGG3050.010.926761
CACCTTT8200.010.88878414
CCACCTT7900.010.82133513
Produced by FastQC (version 0.11.5)