##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512841_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1748894
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.896099477727066	31.0	30.0	34.0	22.0	34.0
2	29.044402919788162	31.0	30.0	34.0	22.0	34.0
3	29.195287421650484	31.0	30.0	34.0	22.0	34.0
4	32.58013235793593	35.0	33.0	37.0	25.0	37.0
5	32.75825521729733	35.0	33.0	37.0	26.0	37.0
6	32.671524975212904	36.0	33.0	37.0	25.0	37.0
7	32.592300047916	36.0	33.0	37.0	25.0	37.0
8	32.51768317576708	36.0	33.0	37.0	23.0	37.0
9	33.81259298733943	38.0	34.0	39.0	22.0	39.0
10	33.66103949124418	38.0	34.0	39.0	21.0	39.0
11	33.718639894699166	38.0	34.0	39.0	22.0	39.0
12	33.61576973790292	38.0	34.0	39.0	19.0	39.0
13	33.586227638724814	38.0	34.0	39.0	19.0	39.0
14	34.42368948604089	39.0	34.0	41.0	17.0	41.0
15	34.61968592722029	39.0	34.0	41.0	19.0	41.0
16	34.5687503073371	39.0	34.0	41.0	18.0	41.0
17	34.3455475288954	39.0	34.0	41.0	17.0	41.0
18	34.3441512178554	39.0	34.0	41.0	17.0	41.0
19	34.44948293035484	39.0	34.0	41.0	18.0	41.0
20	34.42602867869636	39.0	34.0	41.0	18.0	41.0
21	34.3073279455473	39.0	34.0	41.0	18.0	41.0
22	34.333052775068126	39.0	34.0	41.0	17.0	41.0
23	33.98937671465509	39.0	34.0	41.0	10.0	41.0
24	34.06182650292128	39.0	34.0	41.0	2.0	41.0
25	33.7641961148017	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5046444591365784
1101	2	0.46342163304050743
1101	3	0.6770754018995753
1101	4	0.7173859599850303
1101	5	0.571608681884868
1101	6	0.40562827516396993
1101	7	0.2137738523989583
1101	8	0.5225642816489184
1101	9	0.6704869759365977
1101	10	0.607181826228441
1101	11	0.6720445764587168
1101	12	0.6142837024523402
1101	13	0.94948931391977
1101	14	0.9971801634853961
1101	15	0.812266149318269
1101	16	0.7413560885286046
1101	17	0.09790782854238955
1101	18	0.6453431065165702
1101	19	0.08553057154210819
1101	20	-0.5348666109232241
1101	21	0.6157948908149251
1101	22	0.5776494229858713
1101	23	0.18887194476356228
1101	24	0.7413828929176489
1101	25	0.3942550281533812
1102	1	0.6702036221957428
1102	2	0.7217224988413733
1102	3	0.6799938723180468
1102	4	0.8039047190037891
1102	5	0.6991231118992971
1102	6	0.5069017239374247
1102	7	0.5573561034812116
1102	8	0.5674740940587313
1102	9	0.9095995300491566
1102	10	0.8531594597060774
1102	11	0.6107314451455892
1102	12	0.6640131396817779
1102	13	0.5926494870799459
1102	14	0.7701419239471576
1102	15	0.6892358462879642
1102	16	0.6772615719340891
1102	17	0.8542498198843802
1102	18	0.8119412306146998
1102	19	0.6423992584107978
1102	20	0.8420561884995692
1102	21	0.9255604030804392
1102	22	0.6691068544433065
1102	23	0.7012204296120501
1102	24	0.04556398959874741
1102	25	0.464633816032169
1103	1	0.4804687640306504
1103	2	0.5123300889257081
1103	3	0.6722191470433216
1103	4	0.7931054601231367
1103	5	0.5403529866756855
1103	6	0.21629796722901062
1103	7	0.10749602170252359
1103	8	0.23662244570708424
1103	9	0.431397383358636
1103	10	0.6933556007743107
1103	11	0.36866397772928394
1103	12	0.4501586801877835
1103	13	0.6605360191525236
1103	14	0.6895611674943112
1103	15	0.6074597154420687
1103	16	0.6074986023455367
1103	17	0.2606145017141799
1103	18	0.576724644874858
1103	19	0.4725398155281013
1103	20	0.6928976145154166
1103	21	0.5162408273073709
1103	22	0.5938755740957475
1103	23	-0.1223311931837614
1103	24	0.1047136190390745
1103	25	0.4356094102054371
1104	1	1.033437587982693
1104	2	0.9046123156653145
1104	3	0.8730451666612886
1104	4	1.0093940715798624
1104	5	0.8330431048346583
1104	6	0.8040661534676232
1104	7	0.9187214715994969
1104	8	0.7728178645588102
1104	9	1.0398591556231551
1104	10	1.0758514665688708
1104	11	0.8902041201216164
1104	12	1.1744631321125212
1104	13	1.097204162358743
1104	14	1.3678755617329017
1104	15	1.094427853150762
1104	16	1.0646591346327838
1104	17	1.3364491941589947
1104	18	1.0621949269360371
1104	19	0.6224396277906266
1104	20	0.8958071346569128
1104	21	0.927361508192206
1104	22	0.6930146104104011
1104	23	1.0407916311057193
1104	24	0.9212249120390794
1104	25	0.5439569981485306
1105	1	0.6562028394860526
1105	2	0.7667067184040661
1105	3	0.8550508081358501
1105	4	0.9542994824234725
1105	5	0.6425739779297004
1105	6	0.923507414994333
1105	7	0.8579152359358915
1105	8	0.9090916520044985
1105	9	0.5084454119862372
1105	10	0.7129616361439872
1105	11	0.7767815385657784
1105	12	0.9777616878815465
1105	13	0.971826379469988
1105	14	1.201918043726991
1105	15	1.1200145161864654
1105	16	1.0862850784735016
1105	17	1.3704824614033484
1105	18	1.1617807751281575
1105	19	0.9204450090546388
1105	20	1.134601131185022
1105	21	1.1049452185124409
1105	22	1.0616746472019756
1105	23	1.3062382299453361
1105	24	1.3948483152059765
1105	25	1.5780713002517288
1106	1	0.9711578266346308
1106	2	1.0129257394373958
1106	3	0.9135926531870666
1106	4	1.0139945908387347
1106	5	0.9977937902362797
1106	6	1.0509735693057678
1106	7	0.9987990023037625
1106	8	1.029592203884718
1106	9	1.307385817430628
1106	10	0.8900811539855411
1106	11	0.8330102822821956
1106	12	1.0271826197276255
1106	13	1.1103800278739442
1106	14	1.3919547300323956
1106	15	1.0570893421720982
1106	16	1.1662276550850734
1106	17	1.2773554779025176
1106	18	1.2468820975522803
1106	19	1.4100203900144237
1106	20	1.3937632323674478
1106	21	1.3435183586915684
1106	22	1.145712043564906
1106	23	1.37109981900457
1106	24	1.1731166102093553
1106	25	1.2269134346629897
1107	1	0.7347671142592347
1107	2	0.7356213083651824
1107	3	0.7823153008894757
1107	4	0.8747182110672824
1107	5	0.7804425563437434
1107	6	0.8575267239374185
1107	7	0.52323903998915
1107	8	0.7666705226301573
1107	9	0.7436967522713758
1107	10	1.0675443803409976
1107	11	0.9184596197487593
1107	12	0.9395587746024106
1107	13	0.6954669473974064
1107	14	0.9988125588677903
1107	15	0.8767457669228875
1107	16	0.8657337941563128
1107	17	1.2359760103605737
1107	18	1.1070404369639064
1107	19	1.0761194965060383
1107	20	1.1081573789757613
1107	21	0.7918004824455167
1107	22	0.7560314576179081
1107	23	1.184980350246967
1107	24	0.958004465789223
1107	25	1.3930961176194714
1108	1	0.8569540755505685
1108	2	0.8708807700483057
1108	3	0.6941894193662037
1108	4	0.9671361314559377
1108	5	0.7932310103478173
1108	6	0.8283086350798783
1108	7	0.9019909009827245
1108	8	0.7579037736879854
1108	9	0.8434288671436505
1108	10	1.0936717506591265
1108	11	0.7298307802251784
1108	12	0.9403908346243526
1108	13	0.9024701600599982
1108	14	1.070356008170414
1108	15	1.0882707042367912
1108	16	0.8009251826534083
1108	17	1.0846155261104542
1108	18	1.1705091330930344
1108	19	1.0684399595995941
1108	20	1.1626017254731806
1108	21	1.0416207496450127
1108	22	1.044267039815054
1108	23	1.357832861608827
1108	24	1.2051806529072309
1108	25	1.5036631329775645
1109	1	0.8882680771959954
1109	2	0.7506434903012753
1109	3	0.9569460932488667
1109	4	0.9108000901695874
1109	5	0.8607493941773328
1109	6	1.0229549605840447
1109	7	0.9218903144064754
1109	8	0.8205847868678688
1109	9	0.9543483804205763
1109	10	0.9986563266523589
1109	11	0.9044436084686467
1109	12	0.8734596756360986
1109	13	1.0524968770440637
1109	14	1.2932425101313996
1109	15	1.0685238345642816
1109	16	0.6410155514176381
1109	17	1.1781754856583007
1109	18	1.1125864122812814
1109	19	1.0038030686083843
1109	20	0.8933651227559807
1109	21	0.9562856797492927
1109	22	1.0707572662882683
1109	23	1.3569914387585484
1109	24	1.106142214875419
1109	25	0.7197683604760527
1110	1	0.5365829760574279
1110	2	0.5703128572462219
1110	3	0.5331324386986616
1110	4	0.5770861930068207
1110	5	0.4010930765636118
1110	6	0.5918575543261113
1110	7	0.49627962695420536
1110	8	0.493404179873977
1110	9	0.5839634876463293
1110	10	0.5513611255314146
1110	11	0.5441135753828448
1110	12	0.5726135939977794
1110	13	0.6394844189325468
1110	14	0.5409331909840063
1110	15	0.5218809750108377
1110	16	0.6765283563863846
1110	17	0.6995728890413773
1110	18	0.0785780302108634
1110	19	0.5394108687086288
1110	20	0.6460806710841283
1110	21	0.7754859457356602
1110	22	0.7930038761495126
1110	23	0.8517365830699006
1110	24	0.8250907437633117
1110	25	1.046167132538983
1111	1	0.14049093053034056
1111	2	0.2919521177877833
1111	3	0.40240253078674826
1111	4	0.24692493559201978
1111	5	0.21513710410616937
1111	6	0.19393413626472977
1111	7	0.11387437178747462
1111	8	0.3502440702745133
1111	9	0.4010076065635033
1111	10	0.24749109778107226
1111	11	0.022159510526897463
1111	12	-0.324252256806254
1111	13	0.14085453743165033
1111	14	0.2304418834633779
1111	15	0.21399522081355116
1111	16	0.38230673665247394
1111	17	-0.07300158439677773
1111	18	0.25132473853433623
1111	19	0.21798652626161896
1111	20	0.22850032088153682
1111	21	0.32748097896222106
1111	22	-0.01270378267020078
1111	23	0.43072918422973316
1111	24	0.40493813564398806
1111	25	0.5453871938976604
1112	1	0.3661755482796103
1112	2	0.41095668505317207
1112	3	0.30936309762010694
1112	4	0.4819128924224074
1112	5	0.31167997046362217
1112	6	0.12305844035532942
1112	7	0.06453627405690554
1112	8	0.21183976570052465
1112	9	0.07427970062484945
1112	10	0.2797497226769394
1112	11	0.07555731579235214
1112	12	0.08456218019350814
1112	13	0.2475606455731949
1112	14	0.2746994932435314
1112	15	0.02114060819273078
1112	16	0.2348895037038119
1112	17	0.5229740558474276
1112	18	0.1511043436210926
1112	19	0.26546962088414716
1112	20	-0.11106570204768929
1112	21	0.0031492445871919017
1112	22	-0.12308930760360681
1112	23	0.20126129670515525
1112	24	0.2211485667131754
1112	25	0.18906345355881626
1113	1	0.013296051544976706
1113	2	0.25846802645350664
1113	3	0.1570997836480963
1113	4	0.41600803764522
1113	5	0.1652347271443091
1113	6	-0.14795762270505008
1113	7	-0.17593985192454653
1113	8	-0.07738006720649793
1113	9	-0.1253097258492133
1113	10	-0.16203693645674377
1113	11	-0.23181198238876277
1113	12	-0.23054545508099267
1113	13	-0.16318146962215252
1113	14	0.010429064211614048
1113	15	-0.1362010217441849
1113	16	-0.22796075111491376
1113	17	-0.0792879037350076
1113	18	-0.32049913754708825
1113	19	-0.6940124605417566
1113	20	-0.37757500154449986
1113	21	-0.1657041807930426
1113	22	-0.5865892824506531
1113	23	-4.238322609019011
1113	24	-1.7461206098308608
1113	25	-0.8824111671153574
1114	1	-0.7751480388063392
1114	2	-0.9899311027374011
1114	3	-0.8842186592026202
1114	4	-0.8190946779921546
1114	5	-0.6509095051383085
1114	6	-1.3681615914885192
1114	7	-1.141848205256867
1114	8	-1.0739448841234847
1114	9	-1.2266667784696494
1114	10	-1.0784941418726959
1114	11	-1.2222879606213226
1114	12	-2.0664015626534926
1114	13	-1.245100759603531
1114	14	-3.1453840600458243
1114	15	-1.686164877316898
1114	16	-1.9791908455639913
1114	17	-1.5223333398568712
1114	18	-1.585179973200674
1114	19	-1.6137700212734458
1114	20	-1.1380860656392287
1114	21	-1.1185956653331175
1114	22	-1.1384646837652284
1114	23	-5.8831538166328805
1114	24	-3.04210265879507
1114	25	-3.0633710628188346
1115	1	-1.5409078597802264
1115	2	-1.344555872320175
1115	3	-1.1062711477086573
1115	4	-1.0393095667104966
1115	5	-1.01977875725958
1115	6	-1.3730749115765981
1115	7	-1.8310450981209243
1115	8	-2.128697468024047
1115	9	-3.326428507334022
1115	10	-3.7376048927638834
1115	11	-2.7008439887662945
1115	12	-2.9539608256052716
1115	13	-2.2276976424260617
1115	14	-3.1388600787230665
1115	15	-2.7493088800137677
1115	16	-2.8583225402154433
1115	17	-2.791831622038181
1115	18	-5.435819116514807
1115	19	-3.2629946000671737
1115	20	-3.6665252534229893
1115	21	-3.3391892631411935
1115	22	-2.80744855276631
1115	23	-3.2362261658485636
1115	24	-3.133097559132896
1115	25	-3.2004429529931997
1116	1	-1.3075665845711733
1116	2	-1.083430226722534
1116	3	-1.500003778057895
1116	4	-1.252566145657866
1116	5	-1.3223900459954372
1116	6	-3.497571960273106
1116	7	-2.0128812085488654
1116	8	-2.472474214211946
1116	9	-3.3983445488982156
1116	10	-2.7390162921736234
1116	11	-2.1139325586138114
1116	12	-2.0021170294911563
1116	13	-3.3910370730704322
1116	14	-6.3514981136468265
1116	15	-4.340916879275941
1116	16	-3.0675904017501203
1116	17	-4.056485612446444
1116	18	-2.6864445776559798
1116	19	-2.4304320385816816
1116	20	-3.0756693754102002
1116	21	-3.7310796345135486
1116	22	-3.3777587470604544
1116	23	-2.3618763748992286
1116	24	-3.56800978859674
1116	25	-3.626841747877606
1201	1	0.560774383210429
1201	2	0.5410516043153386
1201	3	0.8634484784595671
1201	4	0.7588212744109981
1201	5	0.22321189694602594
1201	6	0.797766209918727
1201	7	0.8526932196360875
1201	8	0.8088726254338994
1201	9	1.1217957917313939
1201	10	1.104264266114619
1201	11	1.193361618710341
1201	12	0.8501513239274416
1201	13	0.9420219837421584
1201	14	1.0798315100619789
1201	15	1.1108780358740802
1201	16	1.111023254177077
1201	17	1.3898506209524726
1201	18	1.086797705915096
1201	19	1.417753063484227
1201	20	1.2280541858293432
1201	21	1.4095957835877826
1201	22	1.1308692042430337
1201	23	1.5641476659271376
1201	24	1.3042856184371985
1201	25	1.5618000376610084
1202	1	0.8364337809259013
1202	2	0.8202277898466654
1202	3	0.9116208564450297
1202	4	1.0977274703265394
1202	5	0.946067556343742
1202	6	1.1392165387522368
1202	7	1.170993669618781
1202	8	1.0883603374449748
1202	9	1.40867360412323
1202	10	1.2268499358965528
1202	11	1.207556841970984
1202	12	1.3356930338616735
1202	13	1.258892873323333
1202	14	1.7799699662752033
1202	15	1.3272781743302957
1202	16	1.1547615719340882
1202	17	1.426763047397614
1202	18	1.548383029556497
1202	19	1.5653093113208527
1202	20	1.4194768234202044
1202	21	1.590388445408479
1202	22	1.4889481242845761
1202	23	1.5910220169136338
1202	24	1.7368470583818194
1202	25	2.0807581546565075
1203	1	1.0450488999294762
1203	2	0.9472923682985908
1203	3	0.9744189819845559
1203	4	1.1565616874515428
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2211	17	0.06130755489548534
2211	18	-0.38044774051779484
2211	19	-0.252582236777954
2211	20	-0.35720546953151455
2211	21	-0.1371135421453218
2211	22	-0.16927952601561458
2211	23	-0.09145351561370774
2211	24	-0.23780573653004922
2211	25	6.016176605925239E-4
2212	1	-0.03282135394170638
2212	2	0.13540555112687613
2212	3	-0.2351819151461605
2212	4	-0.010311793881989217
2212	5	-0.14073026597746718
2212	6	-0.24322262647089588
2212	7	-0.10352336411874319
2212	8	0.005008031910044508
2212	9	0.02378290050201315
2212	10	-0.3224967604657323
2212	11	-0.34207691205029533
2212	12	0.05676290105625981
2212	13	0.0019845173058428145
2212	14	0.174547216130847
2212	15	-0.21706068568785497
2212	16	-0.16295186533391615
2212	17	-0.07940539275746517
2212	18	0.25629031323215656
2212	19	-0.0368328977033201
2212	20	0.04597521490746459
2212	21	0.20439405458112958
2212	22	0.17253881965700657
2212	23	0.21876762443653064
2212	24	0.07928787336160781
2212	25	0.3026397021282534
2213	1	-0.1570672672921276
2213	2	-0.11886375452789721
2213	3	-0.21846300099731764
2213	4	-0.1181774912387965
2213	5	-0.15856556734598115
2213	6	0.025345120163834167
2213	7	-0.21765063506326499
2213	8	-0.111360137747198
2213	9	-0.4557778284414127
2213	10	-0.1455400012103638
2213	11	-0.2720448351778657
2213	12	-0.24379683336404412
2213	13	-0.1951672245103495
2213	14	-0.5044605019288539
2213	15	-0.4182843693454572
2213	16	-0.24863465448100897
2213	17	-0.25228656825577644
2213	18	-0.28500620873840177
2213	19	-0.31296462299081895
2213	20	-0.10169718077266765
2213	21	-0.2927212680785942
2213	22	-0.43849422372381497
2213	23	-0.2387054463987326
2213	24	-0.1134184901856159
2213	25	0.006257805250498905
2214	1	-2.719115743006718
2214	2	-1.3485872834662764
2214	3	-1.5663348542094013
2214	4	-3.4045984383661754
2214	5	-1.7817231463403296
2214	6	-1.428072103127331
2214	7	-1.3525565149201633
2214	8	-1.2057255126548334
2214	9	-1.0386808111683692
2214	10	-1.0996163947225526
2214	11	-1.058199480462342
2214	12	-0.9081334564518286
2214	13	-1.3849254312161818
2214	14	-1.2995230901779138
2214	15	-1.7867398918165023
2214	16	-1.6256649499736255
2214	17	-3.757659281096064
2214	18	-3.479187806110499
2214	19	-2.299541693231589
2214	20	-2.1456526410578434
2214	21	-2.1523500550151695
2214	22	-2.98851126996815
2214	23	-2.083480589609131
2214	24	-3.2218706473037066
2214	25	-2.8914135168210997
2215	1	-1.88133384585581
2215	2	-3.5182134683129043
2215	3	-2.206671661424952
2215	4	-3.8642201110576586
2215	5	-2.366191886197388
2215	6	-2.255419364756907
2215	7	-2.3489320307294577
2215	8	-1.7251297723831627
2215	9	-4.121422548367292
2215	10	-2.3918926094657103
2215	11	-1.846755639524595
2215	12	-1.9240428992545446
2215	13	-2.1878214466304264
2215	14	-1.574785070451668
2215	15	-1.8194525000060153
2215	16	-1.8316716218230482
2215	17	-2.2476499571562414
2215	18	-1.8733615319063759
2215	19	-1.9699331142032648
2215	20	-1.7559180080309602
2215	21	-2.4604571889582836
2215	22	-2.232831260734251
2215	23	-2.4545491880941874
2215	24	-2.1732471203039125
2215	25	-3.0426608981991663
2216	1	-3.0810306943351833
2216	2	-3.158120666467891
2216	3	-4.6157682892492
2216	4	-4.873639304769046
2216	5	-4.4952952240020885
2216	6	-3.721099046478603
2216	7	-5.67062705742568
2216	8	-3.266115494318992
2216	9	-3.863998312785977
2216	10	-2.948532704062359
2216	11	-3.219008814594673
2216	12	-3.561682986226213
2216	13	-3.1129327273158296
2216	14	-3.9487745409438837
2216	15	-3.482344607156893
2216	16	-3.8491983664326312
2216	17	-3.328645843773991
2216	18	-3.0257931959756625
2216	19	-3.8819040653840524
2216	20	-4.3906276107520235
2216	21	-4.154242040238955
2216	22	-3.693402500608425
2216	23	-3.632960177916015
2216	24	-3.9894178505949576
2216	25	-4.045854297549166
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85525.0
3	5.0
4	782.0
5	4809.0
6	5747.0
7	5363.0
8	4689.0
9	4249.0
10	3766.0
11	3637.0
12	3381.0
13	3358.0
14	3242.0
15	3328.0
16	3484.0
17	3819.0
18	4304.0
19	5211.0
20	6705.0
21	8736.0
22	10694.0
23	13120.0
24	15441.0
25	17211.0
26	19419.0
27	21258.0
28	24331.0
29	28423.0
30	34523.0
31	42677.0
32	53540.0
33	67863.0
34	88245.0
35	113855.0
36	154204.0
37	235063.0
38	644887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.228582574623225	18.68979800239804	12.616273399537603	24.465346023441132
2	14.059821288674945	19.461018263414502	43.824360597503805	22.654799850406746
3	19.409943571057696	25.39073668704696	28.790540432355034	26.408779309540307
4	12.0074180014056	16.159085593511595	38.41532001425043	33.41817639083238
5	12.470542352391524	39.3663968085952	35.398470975818	12.764589863195283
6	31.72021965998027	36.359380758463296	18.63178339822871	13.288616183327726
7	28.776390914881233	31.485113272127226	21.587829891801043	18.150665921190505
8	25.117685847475478	38.306113711088116	20.298690802187032	16.277509639249374
9	28.065803961606345	14.35595854803671	20.272271731491635	37.30596575886531
10	16.597920266385387	28.430962379016545	33.88784502212915	21.083272332468926
11	34.38475242957647	21.48111308683407	25.894898533240312	18.239235950349133
12	25.499220433123238	23.957314993097228	31.456782689723223	19.08668188405631
13	30.129153804508935	22.36573280820248	25.52647572872207	21.97863765856651
14	20.498024099971097	23.639376067715713	27.249964946016863	28.612634886296323
15	22.495538938570697	33.99689356748708	22.600097748465956	20.907469745476273
16	20.829596630700845	27.715450118382172	30.283916907561174	21.171036343355805
17	20.82736870422785	29.690316266051553	28.599998282873667	20.882316746846925
18	20.701483857525865	26.59936533666692	32.58562345598454	20.113527349822675
19	23.874856411227942	26.2457667425796	27.706981032831816	22.172395813360637
20	24.62900810893723	27.000963823425717	28.69343546961781	19.67659259801924
21	22.845941049156988	26.610114786239386	26.804916042032207	23.739028122571423
22	23.772462801686267	29.773647652764225	26.73945582709188	19.714433718457634
23	21.267518409855526	29.066222823069644	28.868588030188384	20.79767073688645
24	23.774892617276812	26.23870013249354	29.581715319172076	20.404691931057574
25	22.141225609682813	27.95981825268234	29.34455699520564	20.554399142429208
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	249.0
1	249.0
2	554.0
3	859.0
4	859.0
5	859.0
6	1485.0
7	2111.0
8	2111.0
9	2111.0
10	2321.0
11	2531.0
12	2531.0
13	2531.0
14	3694.0
15	4857.0
16	4857.0
17	4857.0
18	10236.0
19	15615.0
20	15615.0
21	15615.0
22	27511.5
23	39408.0
24	39408.0
25	39408.0
26	63314.5
27	87221.0
28	87221.0
29	87221.0
30	118124.0
31	149027.0
32	149027.0
33	149027.0
34	166268.5
35	183510.0
36	183510.0
37	183510.0
38	192181.0
39	200852.0
40	200852.0
41	200852.0
42	209934.0
43	219016.0
44	219016.0
45	219016.0
46	230757.0
47	242498.0
48	242498.0
49	242498.0
50	227578.0
51	212658.0
52	212658.0
53	212658.0
54	181492.0
55	150326.0
56	150326.0
57	150326.0
58	128189.5
59	106053.0
60	106053.0
61	106053.0
62	86863.0
63	67673.0
64	67673.0
65	67673.0
66	51544.5
67	35416.0
68	35416.0
69	35416.0
70	26399.0
71	17382.0
72	17382.0
73	17382.0
74	12453.5
75	7525.0
76	7525.0
77	7525.0
78	5165.5
79	2806.0
80	2806.0
81	2806.0
82	1873.0
83	940.0
84	940.0
85	940.0
86	605.0
87	270.0
88	270.0
89	270.0
90	168.5
91	67.0
92	67.0
93	67.0
94	41.5
95	16.0
96	16.0
97	16.0
98	12.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33186688272702636
2	0.008691207128619573
3	0.008348133163016169
4	0.010006323996765955
5	0.011264261870645104
6	0.0838244055957651
7	0.08376722660149787
8	0.10692471927972763
9	0.0870264292747302
10	0.0944025195352034
11	0.08994255798235914
12	0.10120681985300423
13	0.08691207128619573
14	0.09085742189063488
15	0.08874179910274722
16	0.09074306390210042
17	0.1022932207440817
18	0.10440884353196936
19	0.09817633315684085
20	0.09640378433455658
21	0.0841674795613685
22	0.10515217045744339
23	0.09903401807084934
24	0.09371637160399658
25	0.09337329763839318
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1748894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.194993321455996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54904111640833	42.80318806505474
2	13.140794919831231	14.506121756774155
3	4.286703937866525	7.0981378566480595
4	1.820882171283352	4.020143171325716
5	0.9513233456461212	2.625414285474142
6	0.5524899450531336	1.8296807296427582
7	0.35291537935956546	1.3635413404753227
8	0.2321236740715596	1.0249651712105248
9	0.1762763796011139	0.8756616235332529
>10	0.8196491706194606	7.9628555764499485
>50	0.05840252316541048	2.2298895573248068
>100	0.04856063177235472	5.455278578151696
>500	0.006564603223765629	2.5083236837606067
>1k	0.0038554018933226707	3.9260243419290184
>5k	4.16800204683532E-4	1.770774262245324
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	8948	0.5116376407032102	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7891	0.4511994437627438	No Hit
CTGTAGGACGTGGAATATGGCAAGA	7856	0.4491981789633906	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6094	0.3484487910645242	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4858	0.277775554150223	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4139	0.2366638572720817	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3261	0.1864607003054502	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2849	0.16290295466734978	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2816	0.16101604785653104	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2778	0.15884324607437617	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2536	0.1450059294617055	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2512	0.14363363359929188	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2408	0.13768701819549956	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2319	0.1325980877057157	No Hit
GAATATGGCAAGAAAACTGAAAATC	2191	0.12527917643950975	No Hit
GCCATATTCCACGTCCTACAGTGGA	2010	0.11492977847714042	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1914	0.10944059502748593	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1898	0.10852573111921018	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.7178994267234036E-5	0.0
5	0.0	0.0	0.0	1.1435798853446807E-4	0.0
6	0.0	0.0	0.0	1.1435798853446807E-4	0.0
7	0.0	0.0	0.0	1.1435798853446807E-4	0.0
8	0.0	0.0	0.0	1.1435798853446807E-4	0.0
9	0.0	0.0	0.0	1.1435798853446807E-4	0.0
10	0.0	0.0	0.0	1.1435798853446807E-4	0.0
11	0.0	0.0	0.0	1.1435798853446807E-4	0.0
12	0.0	0.0	0.0	1.1435798853446807E-4	5.7178994267234036E-5
13	0.0	0.0	0.0	1.1435798853446807E-4	1.715369828017021E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1050	0.0	13.207753	4
GGTATCA	1495	0.0	12.037708	1
TATGTCG	160	3.6379788E-12	11.873748	16
GGCGAGG	1620	0.0	11.668523	19
AGGACTG	265	0.0	11.47016	5
TAGGACT	415	0.0	11.444249	4
GTACTTA	160	4.3655746E-11	11.307245	1
GTCCTAC	3835	0.0	11.297158	1
ATAGGAC	320	0.0	11.279737	3
TCCCGTT	135	4.7384674E-9	11.2577505	11
ACTATAC	85	5.3307776E-5	11.174973	3
GTCCTAA	2120	0.0	11.138815	1
TAGAAAT	785	0.0	11.132265	4
AGCTTCG	60	0.0058807684	11.082165	19
ACTGTTC	395	0.0	11.061809	8
GTCCTAG	330	0.0	10.9646015	1
GCCGGTT	130	3.272544E-8	10.960069	11
CTCTAGG	305	0.0	10.92676	1
CACCTTT	820	0.0	10.888784	14
CCACCTT	790	0.0	10.821335	13
>>END_MODULE