FastQCFastQC Report
Thu 26 May 2016
SRR1512841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748894
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79620.4552591523557174No Hit
CTGTAGGACGTGGAATATGGCAAGA78540.4490838209748561No Hit
GTCCTAAAGTGTGTATTTCTCATTT71930.4112885057642144No Hit
CTTTAGGACGTGAAATATGGCGAGG65940.3770382881981412No Hit
GTATCAACGCAGAGTACTTTTTTTT62730.3586838310383591No Hit
TATCAACGCAGAGTACTTTTTTTTT45500.2601644239159148No Hit
GTCCTACAGTGTGCATTTCTCATTT38920.22254064568807486No Hit
GGTATCAACGCAGAGTACTTTTTTT36900.21099048884609362No Hit
CTGAAGGACCTGGAATATGGCGAGA34630.19801085714743144No Hit
ATTTAGAAATGTCCACTGTAGGACG27620.15792838216610042No Hit
TTTCTAAATTTTCCACCTTTTTCAG25690.14689283627252425No Hit
GTCCTTCAGTGTGCATTTCTCATTT25330.1448343924789038No Hit
CTGTAGGACCTGGAATATGGCGAGA25000.14294748566808507No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22960.13128297083756935No Hit
GAATATGGCAAGAAAACTGAAAATC22130.12653711431338893No Hit
GCCATATTCCACGTCCTACAGTGGA20570.11761719120770041No Hit
GTACTGGTTCACTATCGGTCAGTCA20060.11470106250007148No Hit
GGAATATGGCGAGAAAACTGAAAAT19010.10869726810201191No Hit
ATTCCAGGTCCTTCAGTGTGCATTT18820.10761086721093446No Hit
ACAGTGGACATTTCTAAATTTTCCA17930.10252193672115062No Hit
ACCTGGAATATGGCGAGAAAACTGA17580.10052067192179742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGA250.006058182618.98596211
GGACCGT653.3376582E-614.6301036
TAGGACC9750.014.2399664
GGAAACG500.001508379713.28979217
GAAACGG655.4926688E-513.14375118
GGTATCA16250.012.4719931
ATAAACG853.891233E-612.3089673
AGGACCG853.899968E-612.3064995
TCCAACG2250.012.23504618
GGCGAGG16950.011.92854919
AAATGTC8800.011.7731637
TAGAAAT9500.011.7117824
GCCGGTT1302.6466296E-911.68366811
CCGGTTT1302.6484486E-911.68312
CCCGTAC1604.5474735E-1111.27291416
GAAATGT9550.011.2521576
AATGTCC9150.011.1080688
TTAGAAA10050.011.0730593
TGTAGGA37550.011.0439472
ATTTAGA10500.010.9649261
Produced by FastQC (version 0.11.5)