FastQCFastQC Report
Thu 26 May 2016
SRR1512840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838181
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88530.48161742505226635No Hit
TATCAACGCAGAGTACTTTTTTTTT52290.28446600198783467No Hit
GGTATCAACGCAGAGTACTTTTTTT50780.27625135936015005No Hit
GTACTGGTTCACTATCGGTCAGTCA26390.1435658403606609No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26130.14215139858370857No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19000.10336305293113138No Hit
GGTATCAACGCAGAGTACATGGGGG18830.10243822561543177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG402.7130722E-416.6635681
GGTATCA18050.013.3994341
GTAGGAC6550.011.8915113
CTAACCT1157.0889655E-811.5636434
TGGACCG752.0768304E-411.3984485
TGTAGGA7600.011.3734512
TAGGACC3650.011.1902584
GGACCGT1408.365532E-910.855966
CCGCGAT700.00149332110.85566511
GTATCAA39550.010.8341791
TAGGACG6300.010.7048924
CTGTAGG7300.010.695991
GACGTGA2750.010.3625087
CTAATAC1106.055816E-610.36222653
AGGACCG1856.002665E-1110.2688725
GACGTGG3150.010.2528527
GTGTAGG2900.010.1787331
CTTAGGC1409.7077645E-810.1771863
GTCCTAA3900.010.0103491
TTATGCT1054.1114574E-59.9510264
Produced by FastQC (version 0.11.5)