##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512840_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1838181 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.503572825526973 31.0 30.0 34.0 16.0 34.0 2 28.622752601620842 31.0 28.0 34.0 16.0 34.0 3 28.792784279676486 31.0 29.0 34.0 16.0 34.0 4 32.15376777368496 35.0 33.0 37.0 19.0 37.0 5 32.336247627409925 35.0 33.0 37.0 22.0 37.0 6 32.21960623028962 35.0 33.0 37.0 21.0 37.0 7 32.127185516551414 35.0 33.0 37.0 19.0 37.0 8 32.02345307671008 35.0 33.0 37.0 18.0 37.0 9 33.26069739595829 38.0 33.0 39.0 17.0 39.0 10 33.13345802181613 38.0 33.0 39.0 17.0 39.0 11 33.17432450884869 38.0 33.0 39.0 17.0 39.0 12 33.06867441236744 38.0 33.0 39.0 17.0 39.0 13 33.017774636991675 38.0 33.0 39.0 15.0 39.0 14 33.83139690813908 39.0 33.0 41.0 11.0 41.0 15 33.99121468451692 39.0 33.0 41.0 16.0 41.0 16 33.93208993020818 39.0 33.0 41.0 11.0 41.0 17 33.68097048114413 39.0 33.0 41.0 10.0 41.0 18 33.68807805107332 39.0 33.0 41.0 10.0 41.0 19 33.80263423460475 39.0 33.0 41.0 10.0 41.0 20 33.765208105186595 39.0 33.0 41.0 9.0 41.0 21 33.67135064501265 39.0 33.0 41.0 2.0 41.0 22 33.672977797072214 39.0 33.0 41.0 2.0 41.0 23 33.33356671622653 39.0 33.0 41.0 2.0 41.0 24 33.3857710421335 39.0 33.0 41.0 2.0 41.0 25 33.09962185443109 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5946331650734074 1101 2 0.1681938994194745 1101 3 0.5942796838548752 1101 4 0.6488198240589895 1101 5 0.54064932964706 1101 6 0.6318413458055048 1101 7 0.3302629721462722 1101 8 0.6461352087334191 1101 9 0.7260278360345609 1101 10 0.6670268214209258 1101 11 0.7769148640463825 1101 12 0.6597701328051926 1101 13 0.9994634757238074 1101 14 1.112692742556142 1101 15 1.0041209157805042 1101 16 0.775174785111254 1101 17 -0.10008811742024193 1101 18 0.603295513107021 1101 19 -0.01794696418578212 1101 20 -0.7089030178755493 1101 21 0.3460869111240754 1101 22 0.5298185561064557 1101 23 0.015918753217640358 1101 24 0.44941603385792916 1101 25 0.18805457053110075 1102 1 1.1092768642881232 1102 2 1.1584557907267872 1102 3 1.007011810357728 1102 4 1.3267851000776218 1102 5 1.14791466996936 1102 6 0.9331868513687454 1102 7 1.016733869864467 1102 8 0.990232911338687 1102 9 1.3506454240623853 1102 10 1.303706218915032 1102 11 1.1017458233402095 1102 12 1.1679785936091776 1102 13 1.1853692805914022 1102 14 1.3942064119573345 1102 15 1.3050669591562496 1102 16 1.190045495764224 1102 17 1.4755709951891234 1102 18 1.516977580177695 1102 19 1.2354923078187383 1102 20 1.3749742985179907 1102 21 1.4987740250584807 1102 22 1.3676758799046596 1102 23 1.3114769285523096 1102 24 0.6872324371393645 1102 25 0.9310347533094117 1103 1 0.4526404514430453 1103 2 0.5633167532263172 1103 3 0.7264337567568511 1103 4 0.8194332955483574 1103 5 0.638657126445402 1103 6 0.28855826843089716 1103 7 0.1268364643079991 1103 8 0.4726535107923624 1103 9 0.5389729458086308 1103 10 0.6007805778571154 1103 11 0.3800695686038722 1103 12 0.48438300454083816 1103 13 0.6286122720691409 1103 14 0.635230594034148 1103 15 0.49097023084899405 1103 16 0.6018113790205604 1103 17 0.3990774592684758 1103 18 0.5977246943155876 1103 19 0.7195028064403175 1103 20 0.7700817394475052 1103 21 0.649851833187995 1103 22 0.6761324314725314 1103 23 -0.06206364975305689 1103 24 0.12058255483973568 1103 25 0.5310380262609797 1104 1 0.879960079181128 1104 2 0.6676495163508953 1104 3 0.6695491962395899 1104 4 0.9740147479132801 1104 5 0.7157326348472282 1104 6 0.4996131134555668 1104 7 0.8129159188837747 1104 8 0.5458253330107397 1104 9 0.6538115479120208 1104 10 0.7157974062201617 1104 11 0.35592713580925306 1104 12 0.711204018689017 1104 13 0.8585057438993857 1104 14 0.9598363162951387 1104 15 0.7398606704861237 1104 16 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1107 16 0.8204691911619548 1107 17 1.0473823919504426 1107 18 0.9380300919793498 1107 19 0.8242105087446632 1107 20 0.9819133802669526 1107 21 0.7481401636350569 1107 22 0.8128190784470206 1107 23 0.983871544898463 1107 24 0.8460429911476623 1107 25 1.189745317235264 1108 1 0.9227248589074577 1108 2 0.7663846399135039 1108 3 0.748871232709142 1108 4 0.9077453867372753 1108 5 0.7883531633826806 1108 6 0.9627123902085017 1108 7 0.9321879215992652 1108 8 0.9584248411117144 1108 9 1.112542708217255 1108 10 1.1873555826666546 1108 11 0.7940254170071057 1108 12 0.8693844474034762 1108 13 0.9632829386716821 1108 14 1.0818610288167605 1108 15 1.0547881656316065 1108 16 0.7974215896785068 1108 17 1.1208615589221935 1108 18 1.3711424751928476 1108 19 1.2620525852013813 1108 20 1.2691675657268462 1108 21 1.1939536511949171 1108 22 1.184185288340558 1108 23 1.471534368715588 1108 24 1.3608125711921772 1108 25 1.5040965343410093 1109 1 0.8683695674597729 1109 2 0.8435578064209075 1109 3 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0.038932989985724475 2211 11 0.14007634987400763 2211 12 0.03655039094685719 2211 13 0.024838926776112658 2211 14 0.46905852855174146 2211 15 0.14367660493468293 2211 16 0.07928480106524205 2211 17 0.4938556963231875 2211 18 0.08781929303393099 2211 19 0.1734782644072297 2211 20 0.1718162650514259 2211 21 0.3058070521459584 2211 22 0.27475718988978315 2211 23 0.5718558935592242 2211 24 0.27568006642184173 2211 25 0.7088271472542544 2212 1 0.15911329682457875 2212 2 0.2275433556250661 2212 3 -0.24588895446184722 2212 4 0.23401067499259653 2212 5 -0.07130608943312922 2212 6 -0.15784161923253492 2212 7 -0.04298260665635212 2212 8 -0.03497676757644186 2212 9 0.07318095772608046 2212 10 -0.11939855013025635 2212 11 -0.3308166387242579 2212 12 0.19966840963932952 2212 13 0.16122424995592866 2212 14 0.3104565340134542 2212 15 -0.1183847191337577 2212 16 -0.09480710706099416 2212 17 0.17546223644217918 2212 18 0.2878169140923177 2212 19 0.10458988254529089 2212 20 0.2690102073049019 2212 21 0.4688472145675959 2212 22 0.4631971196317082 2212 23 0.5180220068816723 2212 24 0.4715513051259208 2212 25 0.5940433347744474 2213 1 -0.29836364716982544 2213 2 -0.0993846388250823 2213 3 -0.3661964942062603 2213 4 -0.2135007204768513 2213 5 -0.3266162990349244 2213 6 -0.1454706803302912 2213 7 -0.28356170423976224 2213 8 -0.22840289744425135 2213 9 -0.5771224177124807 2213 10 -0.24719128571938853 2213 11 -0.37857050426035244 2213 12 -0.26510374573916806 2213 13 -0.23916979988428722 2213 14 -0.3795399205694636 2213 15 -0.34403672325392165 2213 16 -0.25003496607525477 2213 17 -0.2157746583481739 2213 18 -0.24596565732816345 2213 19 -0.20480676806704423 2213 20 -0.08815758015813913 2213 21 -0.23448617313839293 2213 22 -0.40388332295532337 2213 23 -0.18978381172826886 2213 24 -0.048813853643039806 2213 25 0.21164863342185924 2214 1 -2.867414722680909 2214 2 -1.4989742127858037 2214 3 -1.6694052463893136 2214 4 -3.726362157235922 2214 5 -1.883610592042583 2214 6 -1.7126506028243185 2214 7 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2215 19 -2.3880434192860207 2215 20 -2.322957932352125 2215 21 -2.948384609149368 2215 22 -2.954377983634952 2215 23 -2.816226301993243 2215 24 -2.6904474701174195 2215 25 -3.8683920830067677 2216 1 -3.1096460533609545 2216 2 -3.0777225009474165 2216 3 -4.3772466519549695 2216 4 -5.041618397153261 2216 5 -4.660358288227815 2216 6 -3.650208982442077 2216 7 -5.280774579891247 2216 8 -3.1960224534210866 2216 9 -4.001912495349547 2216 10 -2.8096166965883853 2216 11 -3.1060603806408658 2216 12 -3.412241907100107 2216 13 -2.899624460420597 2216 14 -3.946586341173255 2216 15 -3.408296138708767 2216 16 -3.64890771958056 2216 17 -3.389329590039665 2216 18 -2.8770359550362414 2216 19 -3.7128842077365682 2216 20 -4.426092533429522 2216 21 -4.02293018009393 2216 22 -3.4368934983074304 2216 23 -3.4309474283958004 2216 24 -4.064000951672288 2216 25 -3.9640373481842133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104717.0 3 1.0 4 894.0 5 5735.0 6 7191.0 7 6790.0 8 6195.0 9 5561.0 10 4700.0 11 4678.0 12 4468.0 13 4089.0 14 4020.0 15 4058.0 16 4178.0 17 4529.0 18 5105.0 19 6072.0 20 7843.0 21 10106.0 22 12599.0 23 15249.0 24 17528.0 25 19837.0 26 21577.0 27 23791.0 28 26661.0 29 31256.0 30 37103.0 31 45742.0 32 56612.0 33 71681.0 34 91100.0 35 119052.0 36 158447.0 37 240239.0 38 648777.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.78591902025613 17.74170041414004 12.537848207603867 23.934532357999966 2 16.010533388829494 19.704673375173083 39.56772843878964 24.71706479720778 3 19.48100632215802 24.901631138532522 28.5176117259165 27.099750813392966 4 13.018166465083054 15.821566127858963 36.46166075517956 34.69860665187843 5 14.15910425169226 38.024469959905915 33.52641318180755 14.290012606594276 6 33.18296948367032 34.93719019783781 17.62142720248103 14.258413116010837 7 29.56827254571866 31.61801697421635 20.578079435151047 18.23563104491394 8 27.196378594189174 34.341052383926964 19.945452357132393 18.517116664751473 9 27.887782848791943 14.72180387262861 19.251032624734425 38.13938065384502 10 17.179057282582715 28.017481487451107 31.748470015905628 23.054991214060554 11 35.41274053003593 22.661211412690434 22.76042094900292 19.165627108270712 12 24.1800017970773 24.641850662876465 29.478275786017104 21.69987175402913 13 29.48416527096868 21.338171269021604 25.52570080736319 23.651962652646528 14 22.750902122131027 21.29079015934088 26.521328851627253 29.436978866900844 15 24.632469045726296 28.792701651983577 23.110237506670007 23.46459179562012 16 23.698045277853254 27.52935295805616 25.3834002984982 23.389201465592393 17 22.732362090147866 27.642854653341114 26.216323071963192 23.40846018454782 18 22.71132868001161 26.66334831131474 27.826642436137544 22.798680572536107 19 24.036524687327613 26.48715084894086 27.16578075930094 22.31054370443059 20 24.692603088236257 25.789157846816686 27.284266723590004 22.233972341357056 21 24.827512234217476 25.594070079688475 26.745839712694618 22.832577973399424 22 24.666014970850682 25.98044367959439 26.585431603253397 22.768109746301533 23 24.108767745413555 25.873262215542447 27.01038450438088 23.007585534663118 24 23.97006172251279 25.729657027572294 27.11102156612368 23.18925968379124 25 23.846092273412626 25.84731526722088 27.138537536816415 23.168054922550077 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 308.0 1 308.0 2 786.0 3 1264.0 4 1264.0 5 1264.0 6 2270.5 7 3277.0 8 3277.0 9 3277.0 10 3833.0 11 4389.0 12 4389.0 13 4389.0 14 5632.5 15 6876.0 16 6876.0 17 6876.0 18 12335.5 19 17795.0 20 17795.0 21 17795.0 22 27012.5 23 36230.0 24 36230.0 25 36230.0 26 52822.0 27 69414.0 28 69414.0 29 69414.0 30 88124.5 31 106835.0 32 106835.0 33 106835.0 34 123379.0 35 139923.0 36 139923.0 37 139923.0 38 155168.5 39 170414.0 40 170414.0 41 170414.0 42 186891.5 43 203369.0 44 203369.0 45 203369.0 46 223764.5 47 244160.0 48 244160.0 49 244160.0 50 241999.0 51 239838.0 52 239838.0 53 239838.0 54 222416.5 55 204995.0 56 204995.0 57 204995.0 58 181720.5 59 158446.0 60 158446.0 61 158446.0 62 135425.5 63 112405.0 64 112405.0 65 112405.0 66 88159.5 67 63914.0 68 63914.0 69 63914.0 70 48262.0 71 32610.0 72 32610.0 73 32610.0 74 23221.0 75 13832.0 76 13832.0 77 13832.0 78 9625.5 79 5419.0 80 5419.0 81 5419.0 82 3600.5 83 1782.0 84 1782.0 85 1782.0 86 1162.0 87 542.0 88 542.0 89 542.0 90 332.5 91 123.0 92 123.0 93 123.0 94 70.5 95 18.0 96 18.0 97 18.0 98 10.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33685474934187654 2 0.011750747070065461 3 0.010934722967977581 4 0.012729975992570915 5 0.013818008128688088 6 0.08296245037893439 7 0.0823096310972641 8 0.10815039433004693 9 0.08698816928256793 10 0.09378837013330026 11 0.09052427372494874 12 0.10151339829973219 13 0.08622654678728592 14 0.09128589622023077 15 0.08709697249617965 16 0.08998025765689016 17 0.10385266739238411 18 0.10499510113530713 19 0.09977254688194472 20 0.0975420810029045 21 0.08318005680615782 22 0.10586552684420088 23 0.09743327778929278 24 0.09444118941497055 25 0.09270033799718308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1838181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.60153220071636 #Duplication Level Percentage of deduplicated Percentage of total 1 75.37717639870255 42.66463677130239 2 13.572324507622493 15.364287253135325 3 4.82144470288382 8.187034728127557 4 2.2025516492118946 4.98671192386432 5 1.171148613594721 3.3144402982102963 6 0.7130255917214182 2.4215004593852814 7 0.4713703316940905 1.867619810549179 8 0.3241610036398221 1.467840758858874 9 0.22644300352556707 1.1535318860111383 >10 1.0309289286089283 9.99018536057051 >50 0.054641063892334425 2.124092478184501 >100 0.029940668128776344 2.973541741408945 >500 0.003487346476981262 1.4513585844578467 >1k 0.0010655780901887189 0.9826690045581236 >5k 2.9061220641510516E-4 1.050548941375608 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8853 0.48161742505226635 No Hit TATCAACGCAGAGTACTTTTTTTTT 5229 0.28446600198783467 No Hit GGTATCAACGCAGAGTACTTTTTTT 5078 0.27625135936015005 No Hit GTACTGGTTCACTATCGGTCAGTCA 2639 0.1435658403606609 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2613 0.14215139858370857 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1900 0.10336305293113138 No Hit GGTATCAACGCAGAGTACATGGGGG 1883 0.10243822561543177 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.4401606805858615E-5 5.4401606805858615E-5 5 0.0 0.0 0.0 5.4401606805858615E-5 5.4401606805858615E-5 6 0.0 0.0 0.0 5.4401606805858615E-5 5.4401606805858615E-5 7 0.0 0.0 0.0 5.4401606805858615E-5 5.4401606805858615E-5 8 0.0 0.0 0.0 5.4401606805858615E-5 5.4401606805858615E-5 9 0.0 0.0 0.0 5.4401606805858615E-5 5.4401606805858615E-5 10 0.0 0.0 0.0 5.4401606805858615E-5 5.4401606805858615E-5 11 0.0 0.0 0.0 5.4401606805858615E-5 5.4401606805858615E-5 12 0.0 0.0 0.0 5.4401606805858615E-5 1.6320482041757585E-4 13 0.0 0.0 0.0 5.4401606805858615E-5 2.7200803402929307E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 40 2.7130722E-4 16.663568 1 GGTATCA 1805 0.0 13.399434 1 GTAGGAC 655 0.0 11.891511 3 CTAACCT 115 7.0889655E-8 11.563643 4 TGGACCG 75 2.0768304E-4 11.398448 5 TGTAGGA 760 0.0 11.373451 2 TAGGACC 365 0.0 11.190258 4 GGACCGT 140 8.365532E-9 10.85596 6 CCGCGAT 70 0.001493321 10.855665 11 GTATCAA 3955 0.0 10.834179 1 TAGGACG 630 0.0 10.704892 4 CTGTAGG 730 0.0 10.69599 1 GACGTGA 275 0.0 10.362508 7 CTAATAC 110 6.055816E-6 10.3622265 3 AGGACCG 185 6.002665E-11 10.268872 5 GACGTGG 315 0.0 10.252852 7 GTGTAGG 290 0.0 10.178733 1 CTTAGGC 140 9.7077645E-8 10.177186 3 GTCCTAA 390 0.0 10.010349 1 TTATGCT 105 4.1114574E-5 9.951026 4 >>END_MODULE