FastQCFastQC Report
Thu 26 May 2016
SRR1512840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838181
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT111020.6039666387586424No Hit
TATCAACGCAGAGTACTTTTTTTTT84690.46072720803881667No Hit
GGTATCAACGCAGAGTACTTTTTTT64480.3507815606841764No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40100.21815044329149302No Hit
GTACTGGTTCACTATCGGTCAGTCA24400.13273992060629503No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23240.12642933421681543No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19730.10733437022795904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT250.006051081718.9898811
GTCGTAT456.70832E-414.79365351
GGTATCA18400.013.9035081
AGCGTAC2400.011.4914991
CCGTACA2650.011.46371617
CCCGTAC2700.011.25234216
AAGTCCG855.2776453E-511.1860385
TAGGACG5950.011.1857344
AGTTCGC855.318803E-511.1775118
AGGGTCG600.005839035411.0928215
ATAGACG1201.2738928E-711.0843668
TTAGGAC4100.010.8998633
CTAATTG700.001480678910.8667343
GTATCAA41100.010.6903041
CGCCCTA3650.010.66467916
CGTGCGC909.538872E-510.55309110
CCCTTAG1303.785317E-710.241761
GTCCTAA4550.010.2417591
GTCTTAT1502.4041583E-810.144221
TCTATAC1606.0827006E-910.1026663
Produced by FastQC (version 0.11.5)