##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512840_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1838181 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.139273553583678 31.0 30.0 34.0 26.0 34.0 2 30.150333944263377 31.0 30.0 34.0 26.0 34.0 3 30.308390196612848 31.0 30.0 34.0 26.0 34.0 4 33.90241983787233 37.0 35.0 37.0 30.0 37.0 5 33.466945311696726 37.0 35.0 37.0 28.0 37.0 6 33.292577281562586 37.0 35.0 37.0 27.0 37.0 7 33.12143961884058 37.0 35.0 37.0 26.0 37.0 8 33.0590692646698 37.0 35.0 37.0 26.0 37.0 9 34.345889768200195 39.0 35.0 39.0 25.0 39.0 10 34.293065263975635 39.0 35.0 39.0 25.0 39.0 11 34.249110397724706 39.0 35.0 39.0 25.0 39.0 12 34.1055130044321 39.0 35.0 39.0 25.0 39.0 13 34.0943231379282 39.0 35.0 39.0 25.0 39.0 14 35.15634368976722 39.0 35.0 41.0 25.0 41.0 15 35.139843682423006 39.0 35.0 41.0 25.0 41.0 16 35.139857826840775 39.0 35.0 41.0 25.0 41.0 17 35.13377844728022 40.0 35.0 41.0 25.0 41.0 18 35.057473121526115 40.0 35.0 41.0 24.0 41.0 19 35.08216981896777 40.0 35.0 41.0 24.0 41.0 20 35.04876288026043 40.0 35.0 41.0 24.0 41.0 21 35.01644778180168 40.0 35.0 41.0 24.0 41.0 22 34.98162096115671 40.0 35.0 41.0 24.0 41.0 23 34.814903973003744 40.0 35.0 41.0 22.0 41.0 24 34.75757066360712 39.0 35.0 41.0 21.0 41.0 25 34.69448764838718 39.0 35.0 41.0 20.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7896225854342234 1101 2 0.5955292489798829 1101 3 0.6165245339475973 1101 4 0.6648872909279007 1101 5 0.7599180650933235 1101 6 0.9090002986319732 1101 7 0.9349988587077718 1101 8 0.6787753678962858 1101 9 1.0388370348001672 1101 10 0.7122808733441204 1101 11 0.9580693821939477 1101 12 1.0647165405290977 1101 13 1.1970454357808435 1101 14 1.1737979727863532 1101 15 0.9346272844052592 1101 16 1.198711248769662 1101 17 1.1059688994839547 1101 18 1.087601636986136 1101 19 0.9031854026190942 1101 20 1.0662976617794797 1101 21 1.1136933755219616 1101 22 1.0621314943278577 1101 23 1.2109944491837368 1101 24 1.3162493746479669 1101 25 1.416713812058802 1102 1 0.6955200113388607 1102 2 0.920116633769144 1102 3 0.5818590104572721 1102 4 0.6705284764537325 1102 5 0.9066911900567831 1102 6 0.9456290326291281 1102 7 0.8252100865181333 1102 8 0.9724872484863241 1102 9 1.0848287727529708 1102 10 0.9837738876273292 1102 11 1.2827493252949438 1102 12 1.1364638465950705 1102 13 1.125366330576142 1102 14 1.0916597783554423 1102 15 1.3076080517623794 1102 16 1.2061792430797595 1102 17 1.2782247891154768 1102 18 0.5996147315515046 1102 19 0.9638987836280748 1102 20 1.1747662601543496 1102 21 1.1638648519731802 1102 22 1.0604849306769637 1102 23 1.1248226609571503 1102 24 1.2222247443940688 1102 25 1.2314510438832755 1103 1 0.7054452369836923 1103 2 0.9190944658163751 1103 3 0.6676335040938994 1103 4 0.5195031614321408 1103 5 0.5838307562648168 1103 6 0.5186509739929406 1103 7 0.7011374018166663 1103 8 0.8519827104130826 1103 9 0.9341678883007489 1103 10 0.7566158166546373 1103 11 0.9857731549000519 1103 12 1.0977340035708636 1103 13 1.0035881108100355 1103 14 1.0777234246752627 1103 15 1.1840908876490346 1103 16 1.1404997909215027 1103 17 1.0829932277916967 1103 18 1.0453549850793706 1103 19 1.0319048008236607 1103 20 1.0111853993680882 1103 21 1.0501180777187216 1103 22 0.9419439117819621 1103 23 0.9236440207426497 1103 24 0.9956973460129177 1103 25 0.9375437691189887 1104 1 0.5693751074824931 1104 2 0.7073856304626069 1104 3 0.557941972620231 1104 4 0.44311659695017624 1104 5 0.6083295180678334 1104 6 0.5222702145242906 1104 7 0.4557246591948996 1104 8 0.6037619469920799 1104 9 0.49608761855289885 1104 10 0.6503967947376239 1104 11 0.7440538828734233 1104 12 0.6772272382601443 1104 13 0.5142152375137101 1104 14 0.7591408360098555 1104 15 0.7159621398645157 1104 16 0.7344845620248819 1104 17 0.7461357161662292 1104 18 0.3433389163084897 1104 19 0.7148869720221498 1104 20 0.6817644803233165 1104 21 0.5927909775698907 1104 22 0.6108517664590138 1104 23 0.6495206703853924 1104 24 0.816519805238876 1104 25 0.7837739070189329 1105 1 0.5281549033053068 1105 2 0.5363223826225472 1105 3 0.48049445146652303 1105 4 0.35238014306032284 1105 5 0.3659182197500286 1105 6 0.48292382664673283 1105 7 0.4012689318593914 1105 8 0.4569518552220728 1105 9 0.720089689052088 1105 10 0.3007635283943486 1105 11 0.62848718303065 1105 12 0.7730105944792598 1105 13 0.7494386482033306 1105 14 0.7380020275448373 1105 15 0.6941035737554486 1105 16 0.7608059161914227 1105 17 0.42527543683292635 1105 18 0.42793072543501154 1105 19 0.7442921540349658 1105 20 0.7504210998531065 1105 21 0.5816393968280522 1105 22 0.6197830347511868 1105 23 0.7515349017973492 1105 24 0.6739147791190447 1105 25 0.4111729477780699 1106 1 -0.09073568163607959 1106 2 0.2448493560257532 1106 3 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0.6367800026038637 1107 16 0.656201809121363 1107 17 0.7356935500002351 1107 18 0.8484927776676798 1107 19 0.7935842197156333 1107 20 0.8441394943376395 1107 21 0.730319808285607 1107 22 0.7952076965550035 1107 23 0.9015473701019587 1107 24 0.8819505166821031 1107 25 0.9965565173700952 1108 1 0.596573435329212 1108 2 0.44558782864823243 1108 3 0.5398520256406485 1108 4 0.47363936766530657 1108 5 0.6826460457992596 1108 6 0.5456207219729379 1108 7 0.47331564344806054 1108 8 0.5885474949456011 1108 9 0.7665427199667718 1108 10 0.5952589245807616 1108 11 0.5923120881436077 1108 12 0.5723867546289654 1108 13 0.6472074115026061 1108 14 0.6503484727706805 1108 15 0.8251783251249876 1108 16 0.7383994638726321 1108 17 0.7173748409121217 1108 18 0.8183004689193041 1108 19 0.9037880972838366 1108 20 0.7516552916343429 1108 21 0.8171578045367269 1108 22 0.7782434500659221 1108 23 0.9111020574875539 1108 24 0.784932027621231 1108 25 0.8042190528819759 1109 1 0.32418855119161094 1109 2 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2216 18 -2.4579601556428585 2216 19 -2.7776617013106133 2216 20 -2.0470942612117895 2216 21 -2.8624830004892843 2216 22 -2.2515118449811027 2216 23 -3.3299547487532273 2216 24 -2.7128162499048187 2216 25 -2.767948902767813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54772.0 3 2.0 4 848.0 5 7639.0 6 10210.0 7 8126.0 8 6427.0 9 5336.0 10 4525.0 11 4016.0 12 3556.0 13 3348.0 14 3294.0 15 3234.0 16 3248.0 17 3456.0 18 3948.0 19 4696.0 20 6199.0 21 8202.0 22 10464.0 23 12640.0 24 14650.0 25 16582.0 26 18096.0 27 19928.0 28 22344.0 29 26454.0 30 33260.0 31 41683.0 32 52570.0 33 67534.0 34 87892.0 35 114849.0 36 156757.0 37 240374.0 38 757022.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.216646648031 18.802671426129056 13.704561962123943 26.276119963715992 2 14.384371309302871 20.194406195745053 38.405564414740475 27.0156580802116 3 17.415361377440835 24.8051656207848 29.321329406265935 28.458143595508428 4 12.152567149904057 16.332125375865914 35.64707693697095 35.86823053725908 5 13.678071861213706 38.07242708430819 33.73372305857414 14.515777995903967 6 33.22771460189395 35.482777034158524 17.156720524780482 14.13278783916704 7 28.94747730796704 31.75681414310354 20.735607327653792 18.560101221275634 8 27.164099002399293 34.60054604595669 19.608394912279937 18.62696003936408 9 27.321437413652284 14.466041235778304 19.21684560606778 38.99567574450163 10 16.51298000095793 28.44366261576846 31.867731134149903 23.17562624912371 11 35.20538346116642 22.660241100195094 22.724765161794355 19.40961027684413 12 23.676338024031416 24.870238284996493 29.65546843421499 21.797955256757103 13 29.164268328112296 21.237292432638878 25.65962924315508 23.938809996093745 14 22.383910545960582 21.076906254199145 26.773649705441215 29.76553349439906 15 24.432896466471085 28.81716229509624 23.13959789677116 23.610343341661515 16 23.48380322303348 27.40639383684617 25.518547132240126 23.591255807880216 17 22.470631310534756 27.558774101693217 26.483477204957083 23.487117382814944 18 22.605632883294597 26.38678522619452 28.228995290697167 22.778586599813718 19 23.80978027884588 26.249627342303235 27.63390107869442 22.306691300156462 20 24.417990411047587 25.459522771993903 27.70693689385618 22.415549923102322 21 24.369742413271407 25.350605518402197 27.244329572179392 23.035322496147 22 24.24771934367583 25.723474942837388 27.140531080521622 22.88827463296516 23 23.697339924632015 25.594922371627167 27.544349549908304 23.16338815383251 24 23.734061009225968 25.559670130416972 27.52552659395348 23.18074226640358 25 23.495455561775472 25.66477403476589 27.684977703501453 23.154792699957184 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 136.0 1 136.0 2 465.5 3 795.0 4 795.0 5 795.0 6 1864.0 7 2933.0 8 2933.0 9 2933.0 10 3469.5 11 4006.0 12 4006.0 13 4006.0 14 5299.5 15 6593.0 16 6593.0 17 6593.0 18 12301.0 19 18009.0 20 18009.0 21 18009.0 22 28022.0 23 38035.0 24 38035.0 25 38035.0 26 55914.0 27 73793.0 28 73793.0 29 73793.0 30 91520.5 31 109248.0 32 109248.0 33 109248.0 34 125745.0 35 142242.0 36 142242.0 37 142242.0 38 157031.0 39 171820.0 40 171820.0 41 171820.0 42 187720.5 43 203621.0 44 203621.0 45 203621.0 46 223247.5 47 242874.0 48 242874.0 49 242874.0 50 241105.5 51 239337.0 52 239337.0 53 239337.0 54 221460.0 55 203583.0 56 203583.0 57 203583.0 58 180359.0 59 157135.0 60 157135.0 61 157135.0 62 133657.0 63 110179.0 64 110179.0 65 110179.0 66 86178.5 67 62178.0 68 62178.0 69 62178.0 70 46689.0 71 31200.0 72 31200.0 73 31200.0 74 22219.0 75 13238.0 76 13238.0 77 13238.0 78 9159.0 79 5080.0 80 5080.0 81 5080.0 82 3358.0 83 1636.0 84 1636.0 85 1636.0 86 1032.0 87 428.0 88 428.0 89 428.0 90 251.5 91 75.0 92 75.0 93 75.0 94 41.0 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08481210501033358 2 0.04303167098343417 3 0.018387743100380213 4 0.005004947826138993 5 0.016592490075786878 6 0.05820971928226872 7 0.07686947041667823 8 0.05293276342210044 9 0.022195855576790314 10 0.04858063487763175 11 0.005820971928226872 12 2.1760642722343446E-4 13 0.005603365501003437 14 4.352128544468689E-4 15 0.01332839366743536 16 0.008758658695743238 17 0.004569734971692124 18 0.006364987996285458 19 0.0017952530245933342 20 0.0013056385633406068 21 2.1760642722343446E-4 22 2.1760642722343446E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1838181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.64407847775972 #Duplication Level Percentage of deduplicated Percentage of total 1 73.5745786547928 40.204150499806026 2 14.546720863404017 15.897843129078272 3 5.236160319757772 8.583754663049254 4 2.3383154795529495 5.111003782818068 5 1.2764527968720918 3.4875293402717236 6 0.7542613375200563 2.4729549432111577 7 0.507245583983809 1.9402577249095825 8 0.3269993324169917 1.4294861746215302 9 0.25173587941115866 1.238028763519009 >10 1.089693478263041 10.249865653863278 >50 0.06064333404820663 2.24694938584981 >100 0.03239475737425524 3.2206957341113798 >500 0.003298750539500103 1.3574458374710319 >1k 0.0011995456507273104 1.1387884117176803 >5k 1.999242751212184E-4 0.8148170921715578 >10k+ 9.99621375606092E-5 0.6064288635307793 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 11102 0.6039666387586424 No Hit TATCAACGCAGAGTACTTTTTTTTT 8469 0.46072720803881667 No Hit GGTATCAACGCAGAGTACTTTTTTT 6448 0.3507815606841764 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4010 0.21815044329149302 No Hit GTACTGGTTCACTATCGGTCAGTCA 2440 0.13273992060629503 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2324 0.12642933421681543 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1973 0.10733437022795904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.6320482041757585E-4 7 0.0 0.0 0.0 0.0 1.6320482041757585E-4 8 0.0 0.0 0.0 0.0 1.6320482041757585E-4 9 0.0 0.0 0.0 0.0 1.6320482041757585E-4 10 0.0 0.0 0.0 0.0 1.6320482041757585E-4 11 0.0 0.0 0.0 0.0 1.6320482041757585E-4 12 0.0 0.0 0.0 0.0 2.1760642722343446E-4 13 0.0 0.0 0.0 0.0 2.1760642722343446E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGTT 25 0.0060510817 18.98988 11 GTCGTAT 45 6.70832E-4 14.7936535 1 GGTATCA 1840 0.0 13.903508 1 AGCGTAC 240 0.0 11.491499 1 CCGTACA 265 0.0 11.463716 17 CCCGTAC 270 0.0 11.252342 16 AAGTCCG 85 5.2776453E-5 11.186038 5 TAGGACG 595 0.0 11.185734 4 AGTTCGC 85 5.318803E-5 11.177511 8 AGGGTCG 60 0.0058390354 11.092821 5 ATAGACG 120 1.2738928E-7 11.084366 8 TTAGGAC 410 0.0 10.899863 3 CTAATTG 70 0.0014806789 10.866734 3 GTATCAA 4110 0.0 10.690304 1 CGCCCTA 365 0.0 10.664679 16 CGTGCGC 90 9.538872E-5 10.553091 10 CCCTTAG 130 3.785317E-7 10.24176 1 GTCCTAA 455 0.0 10.241759 1 GTCTTAT 150 2.4041583E-8 10.14422 1 TCTATAC 160 6.0827006E-9 10.102666 3 >>END_MODULE