FastQCFastQC Report
Thu 26 May 2016
SRR1512839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978455
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69380.35067767525670285No Hit
GTATCAACGCAGAGTACTTTTTTTT57720.2917427993055187No Hit
GTCCTAAAGTGTGTATTTCTCATTT57470.29047918704241443No Hit
CTGTAGGACGTGGAATATGGCAAGA55160.2788034097313308No Hit
CTTTAGGACGTGAAATATGGCGAGG49110.24822399296420694No Hit
GTCCTACAGTGTGCATTTCTCATTT34410.17392359189367462No Hit
TATCAACGCAGAGTACTTTTTTTTT33690.17028438857593425No Hit
GGTATCAACGCAGAGTACTTTTTTT33090.16725171914448395No Hit
GTACTGGTTCACTATCGGTCAGTCA28310.1430914526739299No Hit
CTGTAGGACCTGGAATATGGCGAGA24350.12307583442635794No Hit
CTGAAGGACCTGGAATATGGCGAGA24340.12302528993583377No Hit
GTCCTTCAGTGTGCATTTCTCATTT21510.10872119911749319No Hit
ATTTAGAAATGTCCACTGTAGGACG19820.10017918021890818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG601.4693833E-615.83118254
TAGGACC9350.013.1051714
ATAGGAC2550.012.2924483
GGTATCA17700.011.9511311
GGCGAGG14850.011.8328119
GTCCTAT2750.011.7808211
CGAATAT855.3344454E-511.17438712
TGGACCG600.005882560711.0818285
ATTTCCG1053.4749155E-610.85511914
TTAGGAC19000.010.8485263
GTCCTAA17150.010.8342731
AGGACGT32650.010.7351435
AGGACCT18850.010.733295
CACCTTT7000.010.7194314
TGTAGGA29650.010.6680282
CTAATAG1252.2449058E-710.6385563
GGACGTG32350.010.6291596
TAGAAAT6350.010.62060454
ATTTAGA6550.010.6196671
GGACCTG18800.010.6102616
Produced by FastQC (version 0.11.5)