##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512839_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1978455 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.72233181952584 31.0 30.0 34.0 20.0 34.0 2 28.87133899937072 31.0 30.0 34.0 16.0 34.0 3 29.017709273144956 31.0 30.0 34.0 22.0 34.0 4 32.39170817632951 35.0 33.0 37.0 22.0 37.0 5 32.56242724752395 35.0 33.0 37.0 25.0 37.0 6 32.468991207785876 36.0 33.0 37.0 23.0 37.0 7 32.39364605209621 36.0 33.0 37.0 23.0 37.0 8 32.30824052101261 36.0 33.0 37.0 22.0 37.0 9 33.5824014192893 38.0 34.0 39.0 19.0 39.0 10 33.441639056738715 38.0 33.0 39.0 18.0 39.0 11 33.50601605798464 38.0 34.0 39.0 18.0 39.0 12 33.40205918254396 38.0 34.0 39.0 17.0 39.0 13 33.37566737681676 38.0 34.0 39.0 17.0 39.0 14 34.1942626948806 39.0 33.0 41.0 17.0 41.0 15 34.38773032492526 39.0 34.0 41.0 17.0 41.0 16 34.33176392690256 39.0 34.0 41.0 17.0 41.0 17 34.093473442661065 39.0 33.0 41.0 16.0 41.0 18 34.102252009775306 39.0 33.0 41.0 16.0 41.0 19 34.20538652635516 39.0 34.0 41.0 17.0 41.0 20 34.1798084869254 39.0 34.0 41.0 15.0 41.0 21 34.06344192817122 39.0 34.0 41.0 10.0 41.0 22 34.08841949905356 39.0 34.0 41.0 10.0 41.0 23 33.74650118400469 39.0 33.0 41.0 2.0 41.0 24 33.81712245160997 39.0 34.0 41.0 2.0 41.0 25 33.52326133270658 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6777668933932119 1101 2 0.3735566383120883 1101 3 0.7383768216307978 1101 4 0.8345448809762104 1101 5 0.6657041312182272 1101 6 0.6950661085790131 1101 7 0.40660408515705626 1101 8 0.6406720035517282 1101 9 0.6912405038053961 1101 10 0.775786642307466 1101 11 0.8254444658203184 1101 12 0.6548259701392283 1101 13 1.0873553368516795 1101 14 0.8693399151269503 1101 15 0.8462995799291377 1101 16 0.7987399013706309 1101 17 0.279335462051705 1101 18 0.6986924101024883 1101 19 -0.021037448234444867 1101 20 -0.6567390127077815 1101 21 0.6355126401158628 1101 22 0.6676276317272212 1101 23 0.09218568069139366 1101 24 0.6616437369762025 1101 25 0.28929951890525274 1102 1 0.6936595760472919 1102 2 0.658353791041737 1102 3 0.5881647553622287 1102 4 0.7469881837989973 1102 5 0.6370286277806656 1102 6 0.665927738833382 1102 7 0.6043288255754078 1102 8 0.6132502748531579 1102 9 0.8954778385612201 1102 10 0.8586584245073325 1102 11 0.5996673892685891 1102 12 0.7907639203516723 1102 13 0.6908939982504378 1102 14 0.8882329995480873 1102 15 0.7919168469842859 1102 16 0.8056869542447131 1102 17 0.9082219721028721 1102 18 0.9021222652273622 1102 19 0.7342607138729207 1102 20 0.9454606506375782 1102 21 1.1660618164483125 1102 22 0.9166702221913212 1102 23 0.876466307989098 1102 24 0.10025781198985584 1102 25 0.5586472403990683 1103 1 0.6447169588544668 1103 2 0.7655424563733533 1103 3 0.9699300132336255 1103 4 0.9453090578042591 1103 5 0.7465387822204121 1103 6 0.37193870933290896 1103 7 0.2808043734887775 1103 8 0.47725006865390185 1103 9 0.5530865911955019 1103 10 0.7480342698369213 1103 11 0.5523997041872803 1103 12 0.7526205524537559 1103 13 0.8377533039530647 1103 14 0.9870622945213654 1103 15 0.9322463638838911 1103 16 0.8858722539850419 1103 17 0.596899099013271 1103 18 0.998173986292187 1103 19 1.034762494525836 1103 20 1.0819693651774216 1103 21 0.918399032618531 1103 22 0.8469530297165448 1103 23 0.26176010698326024 1103 24 0.4692365315274074 1103 25 0.8000777390168352 1104 1 0.9166702384962733 1104 2 0.8515896765060482 1104 3 0.9123347458585442 1104 4 1.0814086087774086 1104 5 0.8355315911157177 1104 6 0.7018901139645521 1104 7 0.8625137606060633 1104 8 0.7511604061985224 1104 9 1.0524691471826557 1104 10 1.0184644781247911 1104 11 0.6985163536100174 1104 12 0.9447162421554012 1104 13 1.0249672065843285 1104 14 1.220491325258351 1104 15 0.9641212157272747 1104 16 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13 1.223172798929987 2205 14 1.451311582657432 2205 15 1.2752533885363135 2205 16 1.3416531460950125 2205 17 1.326996497315939 2205 18 1.2509074393718294 2205 19 1.1095256126639228 2205 20 1.3697503686793056 2205 21 1.4523944516261622 2205 22 1.43585595862762 2205 23 1.8421380736631576 2205 24 1.6552958337963233 2205 25 1.9731529215951369 2206 1 0.8557621064381316 2206 2 0.8822138357314238 2206 3 0.9193975427898415 2206 4 0.6891144160070155 2206 5 0.7763955928237181 2206 6 0.8448281028901903 2206 7 0.8158452507796312 2206 8 0.8121709633689775 2206 9 0.9379402557588676 2206 10 0.8816072286683792 2206 11 0.6915304546260757 2206 12 0.6699828718112002 2206 13 0.7547271446946695 2206 14 1.1038675185610174 2206 15 0.9115017119395006 2206 16 1.0204837031928378 2206 17 0.8361332526945588 2206 18 1.214492571873393 2206 19 1.1334170635863003 2206 20 1.095984421165042 2206 21 0.8488341881076238 2206 22 0.7435920316109659 2206 23 1.0493484911754507 2206 24 1.0608567718152315 2206 25 1.2506016968964104 2207 1 1.0742409364859178 2207 2 1.1407554231735553 2207 3 1.1150115673668424 2207 4 1.1144469068012057 2207 5 0.8711109300001851 2207 6 1.1531847122082794 2207 7 1.009406006617965 2207 8 0.9728468361632281 2207 9 1.2451660169397982 2207 10 1.2556140972123018 2207 11 0.994778434623754 2207 12 1.0282904065874874 2207 13 1.0853089823002904 2207 14 1.341561202187151 2207 15 1.2471091441942406 2207 16 1.0118565059535456 2207 17 1.4118437404360193 2207 18 1.299005260306913 2207 19 1.2478821447742376 2207 20 1.2605103699202473 2207 21 1.366215131629545 2207 22 0.8465475613942388 2207 23 1.313614005362922 2207 24 1.2747652264054636 2207 25 1.33634476996545 2208 1 0.5353391269274468 2208 2 0.7946605397587092 2208 3 0.8239888569011846 2208 4 0.7676503188550683 2208 5 -0.07715425199430115 2208 6 0.5907581439879763 2208 7 0.7439423332259025 2208 8 0.7576821661762381 2208 9 0.6038926205569197 2208 10 0.8378302704007865 2208 11 0.6567404819225473 2208 12 0.7641395729342477 2208 13 0.6350477438104889 2208 14 0.8125291639120533 2208 15 0.8026833952524086 2208 16 0.7299937608555069 2208 17 0.9706413038719361 2208 18 1.1694942432549524 2208 19 1.0159920626968244 2208 20 0.9381494270686588 2208 21 0.6900341159568981 2208 22 0.738152491356729 2208 23 0.9848899443341566 2208 24 0.8893493387180698 2208 25 1.1271279702698962 2209 1 0.552385237490796 2209 2 0.6769646807550984 2209 3 0.4803488909242546 2209 4 0.23867048765800547 2209 5 0.05251935163526866 2209 6 0.4927022808643855 2209 7 0.3830963582232272 2209 8 0.3472279008743726 2209 9 0.1987814558232941 2209 10 0.532750649272927 2209 11 0.5996866339349012 2209 12 0.4750506286877325 2209 13 0.3625261492116536 2209 14 0.6729947051704244 2209 15 0.670089568080634 2209 16 0.7717352544649643 2209 17 0.7263933914813379 2209 18 0.7428499471445988 2209 19 0.6287048003329332 2209 20 0.6695954947709168 2209 21 0.5531583325582616 2209 22 0.53575146019854 2209 23 0.6445445096636817 2209 24 0.5602177701100004 2209 25 0.7251565260586759 2210 1 0.10960355956784085 2210 2 0.16390501312476502 2210 3 0.23813770398825795 2210 4 0.20996347600198817 2210 5 0.05716119702211131 2210 6 0.3239653236096771 2210 7 0.25518729086078906 2210 8 0.2532890659981675 2210 9 0.3034994463933387 2210 10 0.20600069267312193 2210 11 0.19976053704175456 2210 12 0.26179137355673987 2210 13 0.21388897930134476 2210 14 0.06101708493587665 2210 15 0.24518866251667504 2210 16 0.1466362561179082 2210 17 0.4710345621099705 2210 18 0.37279866338506906 2210 19 0.21208121356170295 2210 20 0.4174362151052051 2210 21 0.559005347361726 2210 22 0.4477582914848597 2210 23 0.6842054522026828 2210 24 0.5582294754567556 2210 25 0.6872815574744422 2211 1 -0.17209125170990092 2211 2 0.12582640972496506 2211 3 0.07042840813600648 2211 4 0.03338194782006809 2211 5 -0.28622888467282337 2211 6 -0.059030319029332645 2211 7 -0.22341362193395753 2211 8 -0.3631410481936399 2211 9 -0.5677246895476529 2211 10 -0.36690395745304727 2211 11 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0.26318136586135665 2212 22 0.31020510883883645 2212 23 0.3099394293955484 2212 24 0.2642456324012912 2212 25 0.38684778356586236 2213 1 -0.07881626949198761 2213 2 0.06377951674080506 2213 3 -0.2481691993647992 2213 4 -0.08114763510912582 2213 5 0.035321144425140005 2213 6 0.17513860434603146 2213 7 -0.03320140078943723 2213 8 0.08446508177726031 2213 9 -0.29129766077300445 2213 10 0.00257789188416524 2213 11 -0.09319588636390108 2213 12 -0.151865746758844 2213 13 -0.17103409762173527 2213 14 -0.31420311230277775 2213 15 -0.13472570960692565 2213 16 -0.044996827379790716 2213 17 0.011979308987022819 2213 18 -0.03248396646371532 2213 19 0.012998610011401013 2213 20 0.09280978512492766 2213 21 -0.06766920885128513 2213 22 -0.31320802015221716 2213 23 -0.1363232525967888 2213 24 0.08298729268226168 2213 25 0.35270178101158933 2214 1 -2.6573873039747475 2214 2 -1.3934363781688823 2214 3 -1.6626007264583968 2214 4 -3.4885039569482075 2214 5 -1.7408317619788036 2214 6 -1.5402016912204708 2214 7 -1.218034241511937 2214 8 -1.0930765930995854 2214 9 -0.8678073487861369 2214 10 -0.8701703511371832 2214 11 -0.9175064282358179 2214 12 -0.9152123477440668 2214 13 -1.2927549514805179 2214 14 -1.3924171024505654 2214 15 -1.6542937884246598 2214 16 -1.3845310145719054 2214 17 -3.4342798699949135 2214 18 -3.2828427185162603 2214 19 -2.0710825722546033 2214 20 -1.9591652559259742 2214 21 -2.0031568935803534 2214 22 -2.9169797114494287 2214 23 -1.8209855842880849 2214 24 -2.912747617005749 2214 25 -2.8488756245713382 2215 1 -1.8122725441264436 2215 2 -3.592491659355826 2215 3 -1.980536395804723 2215 4 -3.502546236507726 2215 5 -2.1375209661915164 2215 6 -2.0472372888516794 2215 7 -2.285159697038644 2215 8 -1.5723177281400922 2215 9 -3.947023534898875 2215 10 -2.3671976707350346 2215 11 -1.9884257910047118 2215 12 -2.0155490016857343 2215 13 -1.9479037734005011 2215 14 -1.5999850830592948 2215 15 -1.7509018825249143 2215 16 -1.7687114109780069 2215 17 -2.245851892538724 2215 18 -1.9105784102017935 2215 19 -2.104437931628773 2215 20 -1.7992573477422056 2215 21 -2.356393938666926 2215 22 -2.3845399464077843 2215 23 -2.551108961433407 2215 24 -2.3025488293774927 2215 25 -3.1905798973100907 2216 1 -3.300194632252822 2216 2 -3.4552101461805407 2216 3 -4.82806261830541 2216 4 -5.201468662396447 2216 5 -5.279260942252229 2216 6 -4.121980625188478 2216 7 -5.759478126311109 2216 8 -3.622140632508451 2216 9 -4.434855927225652 2216 10 -3.3067527020163148 2216 11 -3.663983430505148 2216 12 -3.979650273243596 2216 13 -3.495297339997336 2216 14 -4.511761763988172 2216 15 -4.038862338820408 2216 16 -4.26112369736374 2216 17 -3.9835206589102476 2216 18 -3.6184527626113905 2216 19 -4.298679849057617 2216 20 -5.07200819240316 2216 21 -4.614802435175527 2216 22 -4.346012386123327 2216 23 -4.346226815999678 2216 24 -4.622952225776803 2216 25 -4.721246983033357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 105314.0 3 6.0 4 939.0 5 5860.0 6 6800.0 7 6453.0 8 5601.0 9 5189.0 10 4580.0 11 4254.0 12 4005.0 13 3898.0 14 3964.0 15 4001.0 16 4075.0 17 4430.0 18 5024.0 19 6145.0 20 7861.0 21 10152.0 22 12840.0 23 15416.0 24 17510.0 25 20262.0 26 22242.0 27 24492.0 28 27377.0 29 32187.0 30 38533.0 31 48386.0 32 60187.0 33 76337.0 34 98244.0 35 127781.0 36 172049.0 37 262134.0 38 723927.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.48851003748844 18.65987966374008 12.66507692432529 24.186533374446192 2 14.543549261941605 19.661101256627763 42.684448883876556 23.110900597554078 3 19.152454210861805 25.501389357175245 28.862941191040782 26.48321524092217 4 12.306436325493813 16.30982320009099 37.90165426076407 33.48208621365112 5 12.937044378155328 38.96283647330431 34.968807549073574 13.131311599466787 6 31.871556690699958 36.18201969708236 18.385697180501616 13.560726431716056 7 28.73351338581801 31.62040660026072 21.652280847477602 17.993799166443665 8 25.517982917746114 37.19686083225389 20.3801509876943 16.9050052623057 9 27.746787032282704 14.651891610240014 20.338736970438926 37.26258438703836 10 16.599783870419387 28.471834349223307 33.26933428851275 21.65904749184455 11 34.2634778887044 22.17721413965423 25.132064742836736 18.42724322880463 12 24.879685096737635 24.427517607059013 31.022221322755605 19.670575973447747 13 29.656106028957733 22.33416281226239 25.61708831142946 22.392642847350412 14 21.077407656987674 23.208435273381454 27.12374257077118 28.59041449885969 15 22.99645574845628 32.516408478278684 22.86011892415828 21.627016849106752 16 21.68994204973453 27.85265303954328 28.83530337254862 21.62210153817357 17 21.24621855499849 29.436705732111534 27.92858021065286 21.388495502237117 18 21.204608230109944 26.797114113349828 31.18448555108666 20.813792105453565 19 23.81270882704719 26.60271912800099 27.582018056013712 22.00255398893811 20 24.621233968278062 26.73011054615365 28.321266853861527 20.32738863170676 21 23.23334058407232 26.582018504560377 26.892316408759566 23.292324502607737 22 23.907866036087135 28.80782722781971 26.683643807905653 20.600662928187504 23 21.86365584238505 28.36783770205407 28.35675758267211 21.41174887288877 24 23.66598670843553 26.384504237596833 28.932425103410313 21.01708395055732 25 22.501253388956314 27.565096545098477 28.687202177836035 21.24644788810917 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 322.0 1 322.0 2 641.5 3 961.0 4 961.0 5 961.0 6 1612.5 7 2264.0 8 2264.0 9 2264.0 10 2650.0 11 3036.0 12 3036.0 13 3036.0 14 4408.0 15 5780.0 16 5780.0 17 5780.0 18 12131.0 19 18482.0 20 18482.0 21 18482.0 22 31287.0 23 44092.0 24 44092.0 25 44092.0 26 69459.5 27 94827.0 28 94827.0 29 94827.0 30 125020.5 31 155214.0 32 155214.0 33 155214.0 34 176100.0 35 196986.0 36 196986.0 37 196986.0 38 209743.5 39 222501.0 40 222501.0 41 222501.0 42 233072.0 43 243643.0 44 243643.0 45 243643.0 46 256614.5 47 269586.0 48 269586.0 49 269586.0 50 256340.5 51 243095.0 52 243095.0 53 243095.0 54 211892.5 55 180690.0 56 180690.0 57 180690.0 58 154997.0 59 129304.0 60 129304.0 61 129304.0 62 107406.5 63 85509.0 64 85509.0 65 85509.0 66 65560.0 67 45611.0 68 45611.0 69 45611.0 70 33962.0 71 22313.0 72 22313.0 73 22313.0 74 15767.5 75 9222.0 76 9222.0 77 9222.0 78 6335.5 79 3449.0 80 3449.0 81 3449.0 82 2283.5 83 1118.0 84 1118.0 85 1118.0 86 728.0 87 338.0 88 338.0 89 338.0 90 216.0 91 94.0 92 94.0 93 94.0 94 52.0 95 10.0 96 10.0 97 10.0 98 9.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3359692285141689 2 0.011321965877414448 3 0.010209987085882671 4 0.011625232820559477 5 0.012737211612091253 6 0.08157880770601302 7 0.08208425261125474 8 0.10791248726910645 9 0.08592563389109178 10 0.09416438584653176 11 0.08961538169935632 12 0.09982536878523898 13 0.08653216777738185 14 0.09113171641508146 15 0.08759360207838945 16 0.08895830332254209 17 0.10250422678302008 18 0.10503145130922867 19 0.09800576712636883 20 0.09628725444854698 21 0.08314568691226235 22 0.10472818436608365 23 0.09815740059794131 24 0.09416438584653176 25 0.09168770581084736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1978455.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.863282842838615 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1446712223077 40.47538680179274 2 13.738864772975958 14.800407184126316 3 4.936373498545307 7.9766784591011595 4 2.216060409248105 4.7745715448058945 5 1.1605434028398494 3.1255338779276594 6 0.7025580720236009 2.2705250488155957 7 0.46322771358956055 1.7465675750401155 8 0.3230680168751056 1.3921203176335017 9 0.22653022777207266 1.0981495557845824 >10 0.9737496496354365 9.04898965156479 >50 0.06031884009412542 2.271979361614087 >100 0.04485688572696509 4.714078800456391 >500 0.0057712844365315746 2.2061400609224084 >1k 0.0030275590486723 2.8771906793471675 >5k 3.784448810840375E-4 1.221681081067494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6938 0.35067767525670285 No Hit GTATCAACGCAGAGTACTTTTTTTT 5772 0.2917427993055187 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5747 0.29047918704241443 No Hit CTGTAGGACGTGGAATATGGCAAGA 5516 0.2788034097313308 No Hit CTTTAGGACGTGAAATATGGCGAGG 4911 0.24822399296420694 No Hit GTCCTACAGTGTGCATTTCTCATTT 3441 0.17392359189367462 No Hit TATCAACGCAGAGTACTTTTTTTTT 3369 0.17028438857593425 No Hit GGTATCAACGCAGAGTACTTTTTTT 3309 0.16725171914448395 No Hit GTACTGGTTCACTATCGGTCAGTCA 2831 0.1430914526739299 No Hit CTGTAGGACCTGGAATATGGCGAGA 2435 0.12307583442635794 No Hit CTGAAGGACCTGGAATATGGCGAGA 2434 0.12302528993583377 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2151 0.10872119911749319 No Hit ATTTAGAAATGTCCACTGTAGGACG 1982 0.10017918021890818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 5.054449052417164E-5 9 0.0 0.0 0.0 0.0 5.054449052417164E-5 10 0.0 0.0 0.0 0.0 5.054449052417164E-5 11 0.0 0.0 0.0 0.0 5.054449052417164E-5 12 0.0 0.0 0.0 0.0 5.054449052417164E-5 13 0.0 0.0 0.0 0.0 5.054449052417164E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACACG 60 1.4693833E-6 15.8311825 4 TAGGACC 935 0.0 13.105171 4 ATAGGAC 255 0.0 12.292448 3 GGTATCA 1770 0.0 11.951131 1 GGCGAGG 1485 0.0 11.83281 19 GTCCTAT 275 0.0 11.780821 1 CGAATAT 85 5.3344454E-5 11.174387 12 TGGACCG 60 0.0058825607 11.081828 5 ATTTCCG 105 3.4749155E-6 10.855119 14 TTAGGAC 1900 0.0 10.848526 3 GTCCTAA 1715 0.0 10.834273 1 AGGACGT 3265 0.0 10.735143 5 AGGACCT 1885 0.0 10.73329 5 CACCTTT 700 0.0 10.71943 14 TGTAGGA 2965 0.0 10.668028 2 CTAATAG 125 2.2449058E-7 10.638556 3 GGACGTG 3235 0.0 10.629159 6 TAGAAAT 635 0.0 10.6206045 4 ATTTAGA 655 0.0 10.619667 1 GGACCTG 1880 0.0 10.610261 6 >>END_MODULE