FastQCFastQC Report
Thu 26 May 2016
SRR1512839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978455
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74110.374585219274636No Hit
GTCCTACAGTGGACATTTCTAAATT65390.33051042353755833No Hit
CTGTAGGACGTGGAATATGGCAAGA55630.28117900078596686No Hit
GTCCTAAAGTGTGTATTTCTCATTT55020.27809578686399233No Hit
TATCAACGCAGAGTACTTTTTTTTT54830.2771354415440331No Hit
CTTTAGGACGTGAAATATGGCGAGG53170.2687450561170206No Hit
GGTATCAACGCAGAGTACTTTTTTT44710.2259844171335714No Hit
GTCCTACAGTGTGCATTTCTCATTT32330.1634103378646469No Hit
CTGAAGGACCTGGAATATGGCGAGA26360.13323527702171642No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25920.13101131943865288No Hit
GTACTGGTTCACTATCGGTCAGTCA25020.12646231529147745No Hit
CTGTAGGACCTGGAATATGGCGAGA23660.1195882645801901No Hit
ATTTAGAAATGTCCACTGTAGGACG21290.10760922032596143No Hit
TTTCTAAATTTTCCACCTTTTTCAG21260.10745758685438891No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG402.7423844E-416.6402195
CCCCGTA1950.014.1196915
CCCGTAC2100.013.11047716
CCGTACA2100.013.10981517
TAGGACC11400.012.4277194
GTCCTAT3100.012.2717711
GGTATCA17650.011.962371
TCCTATA3150.011.4719712
GGCGAGG15650.011.40266819
ACCTTTT7900.011.17680415
AGGACCT21600.011.0934795
CTGCGTT2800.010.85142211
CCACCTT7850.010.76420813
TGGCGAG32600.010.7163818
TTAGGAC20650.010.6839763
GGACCTG21100.010.6803856
TGTCGAG1252.26255E-710.63251218
TGTAGGA32650.010.5727612
TAGAAAT7400.010.5363974
CACCTTT8150.010.48447514
Produced by FastQC (version 0.11.5)