##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512839_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1978455 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.20771308925399 31.0 30.0 34.0 26.0 34.0 2 30.23990436982393 31.0 30.0 34.0 26.0 34.0 3 30.409922894379704 31.0 30.0 34.0 26.0 34.0 4 33.99467159980894 37.0 35.0 37.0 30.0 37.0 5 33.565786939809094 37.0 35.0 37.0 28.0 37.0 6 33.42915305124453 37.0 35.0 37.0 28.0 37.0 7 33.2674223068 37.0 35.0 37.0 27.0 37.0 8 33.21229090376076 37.0 35.0 37.0 26.0 37.0 9 34.528564460652376 39.0 35.0 39.0 27.0 39.0 10 34.449819177085146 39.0 35.0 39.0 27.0 39.0 11 34.428340801281806 39.0 35.0 39.0 26.0 39.0 12 34.29558266425064 39.0 35.0 39.0 25.0 39.0 13 34.28385027711017 39.0 35.0 39.0 25.0 39.0 14 35.35215458526982 40.0 36.0 41.0 25.0 41.0 15 35.35648372088321 40.0 36.0 41.0 25.0 41.0 16 35.35937436029629 40.0 36.0 41.0 25.0 41.0 17 35.35471921271902 40.0 36.0 41.0 25.0 41.0 18 35.280967724815575 40.0 36.0 41.0 25.0 41.0 19 35.28839169958377 40.0 35.0 41.0 25.0 41.0 20 35.267271684218244 40.0 35.0 41.0 25.0 41.0 21 35.228862925868924 40.0 35.0 41.0 25.0 41.0 22 35.194629647881804 40.0 35.0 41.0 25.0 41.0 23 35.0275593834583 40.0 35.0 41.0 24.0 41.0 24 34.99098336833539 40.0 35.0 41.0 24.0 41.0 25 34.92634303029384 40.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6917727589966702 1101 2 0.5144155479717973 1101 3 0.505930822590674 1101 4 0.4918870346647424 1101 5 0.6679443698450314 1101 6 0.6459648406328427 1101 7 0.669919469672628 1101 8 0.4409563805415786 1101 9 0.8211724945107619 1101 10 0.5709234040323352 1101 11 0.8458318632951887 1101 12 0.7722744302730504 1101 13 0.8906660573172616 1101 14 0.8942572888850577 1101 15 0.6386585951122044 1101 16 0.8587698006325937 1101 17 0.9032012519165349 1101 18 0.9130862348086239 1101 19 0.6624460995007908 1101 20 0.8424403651266985 1101 21 0.9725270114864912 1101 22 0.851023528652874 1101 23 0.9398081424585882 1101 24 1.015403819219209 1101 25 1.0060629520882003 1102 1 0.63430068090506 1102 2 0.6998308177351511 1102 3 0.4043218605249699 1102 4 0.47622138872387154 1102 5 0.7192715764186772 1102 6 0.5443508057508026 1102 7 0.6228227664621429 1102 8 0.6570112354973574 1102 9 0.8453431115861818 1102 10 0.8179440826353712 1102 11 0.9326908791327497 1102 12 0.9545524449576135 1102 13 0.8846229803137078 1102 14 0.9150955165467352 1102 15 1.0287015810305178 1102 16 0.9801239675341122 1102 17 1.0431219265965979 1102 18 0.401096577041109 1102 19 0.6923618501671314 1102 20 0.9218382827017706 1102 21 0.9086755850330874 1102 22 0.5974928484851532 1102 23 0.7786792627122878 1102 24 0.8208641310824305 1102 25 0.855094359810515 1103 1 0.6323806687427975 1103 2 0.773665005572191 1103 3 0.5249200305939219 1103 4 0.470666677769799 1103 5 0.5997013446017831 1103 6 0.5895626143279955 1103 7 0.547116621374748 1103 8 0.6672425631060435 1103 9 0.7190084956235623 1103 10 0.52769197783023 1103 11 0.7906418701124025 1103 12 0.8994391402125856 1103 13 0.9106626264897599 1103 14 0.857851631752709 1103 15 0.955069660686334 1103 16 0.934165123508123 1103 17 0.9453668773359425 1103 18 0.9417414215354114 1103 19 0.8370984393518057 1103 20 0.8015944083924538 1103 21 0.9347078045409347 1103 22 0.9153306570413733 1103 23 0.893111113857394 1103 24 0.9416702478611114 1103 25 0.7761956211208982 1104 1 0.7983810050636535 1104 2 0.9813313667260211 1104 3 0.8462160939283585 1104 4 0.7595702503872914 1104 5 0.8168176371649878 1104 6 0.7550301217112221 1104 7 0.8236972010275991 1104 8 0.8570190937296402 1104 9 0.9415262466040701 1104 10 1.0006191117347143 1104 11 1.0664178042585561 1104 12 1.088321917418419 1104 13 0.9074705735889737 1104 14 1.1935864928213888 1104 15 1.2471702069295958 1104 16 1.277147998529415 1104 17 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-2.4722236906969854 2216 18 -2.8592471487135285 2216 19 -2.9699434121583295 2216 20 -2.1951460271429895 2216 21 -2.7764810707260636 2216 22 -2.373728294859305 2216 23 -3.603609520782374 2216 24 -2.9352420131062686 2216 25 -2.982937542008994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56524.0 3 1.0 4 914.0 5 8312.0 6 10171.0 7 7666.0 8 6133.0 9 5189.0 10 4385.0 11 4057.0 12 3628.0 13 3374.0 14 3189.0 15 3325.0 16 3341.0 17 3616.0 18 3864.0 19 4848.0 20 6204.0 21 8327.0 22 10567.0 23 12777.0 24 14690.0 25 17014.0 26 19050.0 27 20709.0 28 23617.0 29 28185.0 30 34947.0 31 44479.0 32 56439.0 33 72260.0 34 94427.0 35 123032.0 36 169237.0 37 260139.0 38 829818.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.81127742107808 19.78187583343282 13.873431527486336 26.533415218002755 2 13.066041933826595 20.12310931678526 41.65019882766516 25.16064992172298 3 17.24072211072297 25.358552947540304 29.75739223190047 27.643332709836255 4 11.496840721831877 16.891068088763078 37.048374867310315 34.56371632209473 5 12.44489494651271 39.14748943398516 35.0278558573133 13.379759762188833 6 31.881257320647922 36.71005277946921 17.92524493193694 13.483444967945928 7 28.24565530254169 31.876813188758803 21.659369648959068 18.218161859740444 8 25.427299853335356 37.597177969959034 20.02816972639458 16.94735245031103 9 27.102841065646082 14.478438589351123 20.314263974660697 38.10445637034209 10 16.017797215316367 28.89296384927996 33.36113094648091 21.72810798892276 11 34.05582818240455 22.115328233964384 25.176162775415463 18.652680808215607 12 24.38732217103727 24.658696775145746 31.094050583134443 19.859930470682546 13 29.333870488352765 22.274410056188977 25.78657489875268 22.605144556705575 14 20.67491866029192 23.06403407548709 27.254273714878103 29.00677354934289 15 22.773567574644954 32.51498510462654 22.917036333791845 21.794410986936658 16 21.595022618716033 27.80709412307675 28.688892944143475 21.908990314063743 17 20.92963068925672 29.44315772766378 28.104101734085347 21.523109848994153 18 21.051524838397256 26.71939859718507 31.379997452368386 20.84907911204929 19 23.626165333268634 26.451068639165708 27.949953598961585 21.972812428604065 20 24.319609952163944 26.670521586879254 28.478981249722 20.530887211234802 21 22.735761938530572 26.39787671845645 27.352564915815286 23.513796427197693 22 23.652666614437095 28.47706153064654 27.136766500964143 20.733505353952218 23 21.506124728639268 28.16339012006844 28.69981879800147 21.630666353290824 24 23.6137794390067 26.196046915396103 29.051608452049706 21.13856519354749 25 22.19536061995882 27.54352641001509 28.890335585260008 21.370777384766086 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 114.0 1 114.0 2 316.5 3 519.0 4 519.0 5 519.0 6 1311.0 7 2103.0 8 2103.0 9 2103.0 10 2536.0 11 2969.0 12 2969.0 13 2969.0 14 4393.5 15 5818.0 16 5818.0 17 5818.0 18 12329.5 19 18841.0 20 18841.0 21 18841.0 22 32508.5 23 46176.0 24 46176.0 25 46176.0 26 72273.0 27 98370.0 28 98370.0 29 98370.0 30 128243.0 31 158116.0 32 158116.0 33 158116.0 34 178464.5 35 198813.0 36 198813.0 37 198813.0 38 210784.5 39 222756.0 40 222756.0 41 222756.0 42 233034.5 43 243313.0 44 243313.0 45 243313.0 46 255948.5 47 268584.0 48 268584.0 49 268584.0 50 255401.5 51 242219.0 52 242219.0 53 242219.0 54 210866.5 55 179514.0 56 179514.0 57 179514.0 58 153521.0 59 127528.0 60 127528.0 61 127528.0 62 105577.0 63 83626.0 64 83626.0 65 83626.0 66 63925.5 67 44225.0 68 44225.0 69 44225.0 70 32895.0 71 21565.0 72 21565.0 73 21565.0 74 15105.0 75 8645.0 76 8645.0 77 8645.0 78 5942.0 79 3239.0 80 3239.0 81 3239.0 82 2156.0 83 1073.0 84 1073.0 85 1073.0 86 668.5 87 264.0 88 264.0 89 264.0 90 162.0 91 60.0 92 60.0 93 60.0 94 32.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08547073347637424 2 0.043619895322360124 3 0.01844873904132265 4 0.007834396031246605 5 0.02097596356753123 6 0.05918759840380499 7 0.08188207464915805 8 0.05837888655541824 9 0.02527224526208582 10 0.053930971389291135 11 0.00864310787963335 12 4.5490041471754475E-4 13 0.006621328258666484 14 0.0012130677725801194 15 0.01637641492983161 16 0.01142305485846279 17 0.0061158833534247685 18 0.0074300401070532315 19 0.0025777690167327534 20 0.001566879206249321 21 4.0435592419337316E-4 22 5.054449052417164E-4 23 0.0 24 0.0 25 5.0544490524171645E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1978455.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.09622184078787 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48588446477673 38.283369392435155 2 14.679367676141943 15.294791898775628 3 5.183201497965587 8.100756452505577 4 2.395816196524023 4.992518882554724 5 1.2944082385931284 3.3716889375145547 6 0.7465666003957402 2.3335979539883587 7 0.4858712674691367 1.771844013529585 8 0.3236797227897967 1.3489992515057572 9 0.23798309633181222 1.1158218162773712 >10 1.0426891579702346 9.332890458041016 >50 0.06719897462125526 2.4199331836541256 >100 0.04818487968988247 5.009923687106192 >500 0.005060721170211201 1.8600116142055332 >1k 0.0035035761947615996 2.9480012895377175 >5k 5.839293657936E-4 1.815851168368619 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7411 0.374585219274636 No Hit GTCCTACAGTGGACATTTCTAAATT 6539 0.33051042353755833 No Hit CTGTAGGACGTGGAATATGGCAAGA 5563 0.28117900078596686 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5502 0.27809578686399233 No Hit TATCAACGCAGAGTACTTTTTTTTT 5483 0.2771354415440331 No Hit CTTTAGGACGTGAAATATGGCGAGG 5317 0.2687450561170206 No Hit GGTATCAACGCAGAGTACTTTTTTT 4471 0.2259844171335714 No Hit GTCCTACAGTGTGCATTTCTCATTT 3233 0.1634103378646469 No Hit CTGAAGGACCTGGAATATGGCGAGA 2636 0.13323527702171642 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2592 0.13101131943865288 No Hit GTACTGGTTCACTATCGGTCAGTCA 2502 0.12646231529147745 No Hit CTGTAGGACCTGGAATATGGCGAGA 2366 0.1195882645801901 No Hit ATTTAGAAATGTCCACTGTAGGACG 2129 0.10760922032596143 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2126 0.10745758685438891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.054449052417164E-5 0.0 6 0.0 0.0 0.0 5.054449052417164E-5 1.0108898104834328E-4 7 0.0 0.0 0.0 5.054449052417164E-5 1.0108898104834328E-4 8 0.0 0.0 0.0 5.054449052417164E-5 1.0108898104834328E-4 9 0.0 0.0 0.0 5.054449052417164E-5 1.0108898104834328E-4 10 0.0 0.0 0.0 5.054449052417164E-5 1.0108898104834328E-4 11 0.0 0.0 0.0 5.054449052417164E-5 1.0108898104834328E-4 12 0.0 0.0 0.0 5.054449052417164E-5 1.0108898104834328E-4 13 0.0 0.0 0.0 5.054449052417164E-5 1.0108898104834328E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTCCG 40 2.7423844E-4 16.640219 5 CCCCGTA 195 0.0 14.11969 15 CCCGTAC 210 0.0 13.110477 16 CCGTACA 210 0.0 13.109815 17 TAGGACC 1140 0.0 12.427719 4 GTCCTAT 310 0.0 12.271771 1 GGTATCA 1765 0.0 11.96237 1 TCCTATA 315 0.0 11.471971 2 GGCGAGG 1565 0.0 11.402668 19 ACCTTTT 790 0.0 11.176804 15 AGGACCT 2160 0.0 11.093479 5 CTGCGTT 280 0.0 10.851422 11 CCACCTT 785 0.0 10.764208 13 TGGCGAG 3260 0.0 10.71638 18 TTAGGAC 2065 0.0 10.683976 3 GGACCTG 2110 0.0 10.680385 6 TGTCGAG 125 2.26255E-7 10.632512 18 TGTAGGA 3265 0.0 10.572761 2 TAGAAAT 740 0.0 10.536397 4 CACCTTT 815 0.0 10.484475 14 >>END_MODULE