FastQCFastQC Report
Thu 26 May 2016
SRR1512838_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512838_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092792
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61570.294200283640228No Hit
GTCCTAAAGTGTGTATTTCTCATTT54210.25903195348606073No Hit
CTGTAGGACGTGGAATATGGCAAGA47460.22677838982564918No Hit
CTTTAGGACGTGAAATATGGCGAGG43540.20804743137397314No Hit
GTATCAACGCAGAGTACTTTTTTTT37880.18100222095650212No Hit
GTCCTACAGTGTGCATTTCTCATTT27860.133123597567269No Hit
TATCAACGCAGAGTACTTTTTTTTT22660.10827640778443343No Hit
CTGAAGGACCTGGAATATGGCGAGA22180.10598282103524861No Hit
GGTATCAACGCAGAGTACTTTTTTT22040.10531385823340303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.012.4383954
GGTATCA15800.012.2984711
TGCGTAT550.00307400612.08830610
CAAGTCG1950.011.691196
GCGCCAC1552.5465852E-1111.6434913
AAGTCGT2150.011.4872737
CGTCTAG600.005761660611.1128341
TCCAACG600.005882040611.08200818
GTCCTAA15000.010.9223291
TGTAGGA30750.010.8111982
TTAGGAC14850.010.8096383
TGCGCCA1855.456968E-1210.78223612
GACGTGA14050.010.7498617
AGGACCT16800.010.74224955
CTGTAGG30100.010.60123351
AGGACGT29400.010.532255
GTCCTAC28700.010.4877721
CCGTACA1451.4466423E-810.48150617
GGACGTG28550.010.4806096
CACCTTT7750.010.4178614
Produced by FastQC (version 0.11.5)