##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512838_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2092792
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.33911826880072	31.0	28.0	33.0	16.0	34.0
2	28.485515044017752	31.0	28.0	34.0	16.0	34.0
3	28.654858198999232	31.0	28.0	34.0	16.0	34.0
4	32.039654681401686	35.0	33.0	37.0	19.0	37.0
5	32.196064874101204	35.0	33.0	37.0	22.0	37.0
6	32.103765687177706	35.0	33.0	37.0	19.0	37.0
7	32.00931387352398	35.0	33.0	37.0	18.0	37.0
8	31.942891123437015	35.0	33.0	37.0	18.0	37.0
9	33.19245343063238	38.0	33.0	39.0	17.0	39.0
10	33.05510246598802	38.0	33.0	39.0	17.0	39.0
11	33.10793141411091	38.0	33.0	39.0	17.0	39.0
12	32.9981660862618	38.0	33.0	39.0	17.0	39.0
13	32.95800347096128	38.0	33.0	39.0	16.0	39.0
14	33.768675052274666	39.0	33.0	41.0	11.0	41.0
15	33.92797994258388	39.0	33.0	41.0	16.0	41.0
16	33.86061252145459	39.0	33.0	41.0	11.0	41.0
17	33.624301889533214	39.0	33.0	41.0	10.0	41.0
18	33.60010502715989	38.0	33.0	41.0	10.0	41.0
19	33.70027742843054	39.0	33.0	41.0	10.0	41.0
20	33.676541672559914	39.0	33.0	41.0	10.0	41.0
21	33.57471311052412	39.0	33.0	41.0	2.0	41.0
22	33.560761891291634	39.0	33.0	41.0	2.0	41.0
23	33.209525839166055	39.0	33.0	41.0	2.0	41.0
24	33.26023369737652	39.0	33.0	41.0	2.0	41.0
25	32.961763997568795	39.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8887459963853388
1101	2	0.5055731353502466
1101	3	0.8368286859875447
1101	4	0.8928197242861131
1101	5	0.7364066616716016
1101	6	0.7374329476978048
1101	7	0.42074324066936697
1101	8	0.7324959535837365
1101	9	0.798482667291978
1101	10	0.8251371129720795
1101	11	0.9462407542520737
1101	12	0.7561883962918685
1101	13	1.3246273505226256
1101	14	1.3444470846573822
1101	15	1.1816247001238551
1101	16	1.0038671839792457
1101	17	0.2667274124328074
1101	18	0.7581140040972301
1101	19	9.520042648958338E-4
1101	20	-0.5767048712539591
1101	21	0.7901106662352646
1101	22	0.8517395176828018
1101	23	0.3434496140895362
1101	24	0.9915348062586915
1101	25	0.47568702246172023
1102	1	1.0524671735269706
1102	2	1.1378185934381548
1102	3	1.031857854748452
1102	4	1.1598169514184278
1102	5	1.072646517550254
1102	6	0.912638653819009
1102	7	1.0016889523250114
1102	8	0.9591956762969716
1102	9	1.3066245070981637
1102	10	1.3599323669068966
1102	11	1.162090714437987
1102	12	1.2836793181150767
1102	13	1.1665851871210862
1102	14	1.537666830186069
1102	15	1.3782757988232675
1102	16	1.3863410789265274
1102	17	1.6376343265303817
1102	18	1.6128778585527357
1102	19	1.2991092614413162
1102	20	1.6286804782911446
1102	21	1.667593964084169
1102	22	1.3597288780031178
1102	23	1.2161755519795605
1102	24	0.6688814373091603
1102	25	1.1245437086871775
1103	1	0.586033152905383
1103	2	0.6557754282484041
1103	3	0.9190519242895228
1103	4	0.9425333263049112
1103	5	0.6401142204882362
1103	6	0.34595218480911427
1103	7	0.23423627957545534
1103	8	0.5728692852593298
1103	9	0.5635625554323909
1103	10	0.714144031424695
1103	11	0.5566687628897711
1103	12	0.6738246365262555
1103	13	0.807213381823324
1103	14	0.8298570519149351
1103	15	0.8485705626498827
1103	16	0.8845335518249584
1103	17	0.45699304128973495
1103	18	0.8237020292788841
1103	19	0.7512846712973413
1103	20	1.0477808249511043
1103	21	0.8295620455058099
1103	22	0.782478453652196
1103	23	0.18858751516987127
1103	24	0.3224277011107972
1103	25	0.636998822792755
1104	1	1.0574952978845964
1104	2	0.9324499452968098
1104	3	0.9434337946667668
1104	4	1.1186754492517395
1104	5	0.7906911635404796
1104	6	0.7352481872391365
1104	7	0.9982031515438834
1104	8	0.6268037666437465
1104	9	1.0062368498395031
1104	10	1.0676454382445542
1104	11	0.6882427333910428
1104	12	1.040918666884103
1104	13	1.1407049642856464
1104	14	1.483329200854513
1104	15	1.1127166649994962
1104	16	1.0354724318233721
1104	17	1.3837991774162646
1104	18	0.9968778087199865
1104	19	0.6707477829867088
1104	20	0.888754272286711
1104	21	0.9384137750934727
1104	22	0.7114280709202276
1104	23	1.0713831780512209
1104	24	0.926284195967078
1104	25	0.5051795330350402
1105	1	0.6902940976435623
1105	2	0.7200285983869819
1105	3	0.8690676309699619
1105	4	1.051131967159023
1105	5	0.7722194212643672
1105	6	0.8503516301436491
1105	7	0.8723008186983492
1105	8	0.9615627961209725
1105	9	0.6229943134285065
1105	10	0.7401362253288468
1105	11	0.746248644414159
1105	12	0.9351109432018845
1105	13	0.8914258057661044
1105	14	1.0631341616868113
1105	15	1.0269244362322425
1105	16	0.9621126177623509
1105	17	1.3518066871210834
1105	18	1.1551620543266878
1105	19	0.8891920607310126
1105	20	1.0991280768622218
1105	21	1.0521107880181972
1105	22	0.9609236663471776
1105	23	1.2409192734146401
1105	24	1.3071795397041441
1105	25	1.6294405526660327
1106	1	0.6380115090621246
1106	2	0.6358735917496574
1106	3	0.5577692251950239
1106	4	0.6328846937004613
1106	5	0.5657720496190493
1106	6	0.7320600796970353
1106	7	0.639812663215146
1106	8	0.6610200650250917
1106	9	0.6998546017866119
1106	10	0.49490914099386174
1106	11	0.22600890649336947
1106	12	0.4972459221069414
1106	13	0.5047059447485296
1106	14	0.8041200344546837
1106	15	0.5578274079448207
1106	16	0.7448986711871015
1106	17	0.8292138469804442
1106	18	0.7828317919348393
1106	19	0.7820739434155826
1106	20	0.9102391504186329
1106	21	0.9055307206056398
1106	22	0.8233078300571108
1106	23	1.022183601118428
1106	24	0.6454687411044446
1106	25	0.9701621154747144
1107	1	0.9034941240877963
1107	2	0.9326007355164592
1107	3	0.8291815024219602
1107	4	1.045173952218029
1107	5	0.9337703110967226
1107	6	0.9502763285745743
1107	7	0.6410590522880071
1107	8	0.8101897115139352
1107	9	0.8104839332603859
1107	10	1.0634485913944474
1107	11	0.9458118308153729
1107	12	0.9343271296530844
1107	13	0.7537647015258386
1107	14	1.1217287796401862
1107	15	0.8867446799384311
1107	16	0.9128229840633679
1107	17	1.1434262835885178
1107	18	1.0060331822216497
1107	19	0.9121174380047705
1107	20	1.0302809970957725
1107	21	0.8513934075453307
1107	22	0.8209588500781635
1107	23	1.1698537803989808
1107	24	1.016933592202399
1107	25	1.3066096651556123
1108	1	0.8621117798415021
1108	2	0.8868414941636793
1108	3	0.8878401870819843
1108	4	1.0591881582766618
1108	5	0.9733241305483986
1108	6	0.9543427063627234
1108	7	0.9781453081168507
1108	8	0.9017684083833473
1108	9	1.0575067752472123
1108	10	1.1328210733293957
1108	11	0.9227121722104599
1108	12	1.0366562967855018
1108	13	1.062714127713683
1108	14	1.1461142348448519
1108	15	1.1860167611609356
1108	16	1.0263369321299294
1108	17	1.3173462480749762
1108	18	1.4365005562339164
1108	19	1.2099600734121765
1108	20	1.3550669085188147
1108	21	1.4051611326894502
1108	22	1.4434113985946055
1108	23	1.6608573679460505
1108	24	1.4745955082491164
1108	25	1.7002991567270698
1109	1	0.7960697192806983
1109	2	0.6367133772986868
1109	3	0.8548956149692444
1109	4	0.9919356199149334
1109	5	0.7232840064884272
1109	6	0.8202019593993768
1109	7	0.7529011939951289
1109	8	0.5865414347327444
1109	9	0.7633199688290802
1109	10	0.899128166900681
1109	11	0.760394777878453
1109	12	0.6517451184366152
1109	13	0.7600243161929541
1109	14	0.8421185375941462
1109	15	0.65197491547395
1109	16	0.296210370793176
1109	17	0.8359239350110741
1109	18	0.8598710168185804
1109	19	0.7281416279451349
1109	20	0.6467385048697807
1109	21	0.5877424131594324
1109	22	0.7143420691623987
1109	23	1.0321776126517364
1109	24	0.7811373943073363
1109	25	0.33456629035980257
1110	1	0.7699779711335744
1110	2	0.816424584616243
1110	3	0.7267788644802948
1110	4	0.7781882988444195
1110	5	0.6476402716915288
1110	6	0.8260905205996494
1110	7	0.609407413245151
1110	8	0.7804300919394436
1110	9	0.7607074604576809
1110	10	0.8848503769554199
1110	11	0.8441007195098749
1110	12	0.7918888413993841
1110	13	0.8134355614130513
1110	14	0.7859144385861825
1110	15	0.7480815212832042
1110	16	0.9737832681773
1110	17	0.9627439319508682
1110	18	0.23410592389068796
1110	19	0.7020535467103599
1110	20	0.7742635490477383
1110	21	0.9308060650907777
1110	22	0.9823280242213883
1110	23	1.180151193548319
1110	24	0.9643855052641044
1110	25	1.1983338294515846
1111	1	0.3940700524263079
1111	2	0.4139753288130059
1111	3	0.46063424215618554
1111	4	0.36743603449050966
1111	5	0.48146071441237837
1111	6	0.4258634601713993
1111	7	0.3120740309156247
1111	8	0.5468140740779788
1111	9	0.6358461221938541
1111	10	0.6342174116136832
1111	11	0.28662223127055597
1111	12	-0.11106111520039263
1111	13	0.21508602829472778
1111	14	0.3733458753810126
1111	15	0.4440164483015323
1111	16	0.5143037889396922
1111	17	0.03423096801189729
1111	18	0.3361007232470925
1111	19	0.46105462512993967
1111	20	0.5492668336382565
1111	21	0.4796766609498988
1111	22	0.26971056550277694
1111	23	0.47293271231948353
1111	24	0.46815443654476496
1111	25	0.5580469970267785
1112	1	0.18820207197289207
1112	2	0.2914389772472177
1112	3	0.3548172246293717
1112	4	0.4420961254056408
1112	5	0.2775345237292228
1112	6	0.13485790538284448
1112	7	-0.10413226496591932
1112	8	0.1457029459224124
1112	9	0.01742200156000706
1112	10	0.1544561703681424
1112	11	0.1419297047536361
1112	12	0.15289693157300377
1112	13	0.20334708460791262
1112	14	0.12449577967638703
1112	15	-0.21074040399631144
1112	16	0.034482295538452945
1112	17	0.4092942455587192
1112	18	0.10979601267049333
1112	19	0.32412729758149084
1112	20	0.07386207039428427
1112	21	-0.06479130484528639
1112	22	-0.15316420375788908
1112	23	0.10244007637715669
1112	24	0.10987227919374476
1112	25	0.12361940955036488
1113	1	-0.0751788560161799
1113	2	0.14725811755540974
1113	3	0.0933518214620861
1113	4	0.10812570665804344
1113	5	0.04363880511395024
1113	6	-0.4245340355400202
1113	7	-0.360228862908464
1113	8	-0.25744406393800645
1113	9	-0.334998282727355
1113	10	-0.2645614161773011
1113	11	-0.188447637629082
1113	12	-0.3310809571878295
1113	13	-0.18604336231231144
1113	14	0.06559792677161624
1113	15	-0.3252930569181558
1113	16	-0.3116887891074711
1113	17	-0.1369024505611378
1113	18	-0.2501241649023882
1113	19	-0.7657668416595556
1113	20	-0.3991807861387784
1113	21	-0.14047107604945097
1113	22	-0.7406337901325841
1113	23	-4.4720498284335335
1113	24	-1.9911596497770994
1113	25	-1.0412992740198739
1114	1	-0.237686629372309
1114	2	-0.4483083502552141
1114	3	-0.3727610748608505
1114	4	-0.16435111514471146
1114	5	-0.20867931017526686
1114	6	-0.8300842118149099
1114	7	-0.6602257299208247
1114	8	-0.8003373248224754
1114	9	-0.7730124178217821
1114	10	-0.6510405646667223
1114	11	-0.6370713693602141
1114	12	-1.7504473133931526
1114	13	-0.7409652267828122
1114	14	-2.6551949437307734
1114	15	-1.3472295861353345
1114	16	-1.45033945270972
1114	17	-1.2865439894846702
1114	18	-1.2830636876005883
1114	19	-1.1511390997042383
1114	20	-0.6814130220988517
1114	21	-0.6083662285491087
1114	22	-0.8961671192097853
1114	23	-5.241682133910469
1114	24	-2.347606451646964
1114	25	-2.1079237340228545
1115	1	-1.816549831259369
1115	2	-1.7126745930635359
1115	3	-1.4188699275233105
1115	4	-1.354820461837111
1115	5	-1.356810729074148
1115	6	-1.5334472471698959
1115	7	-1.9266633579566133
1115	8	-2.2950927051771686
1115	9	-3.7853084160332706
1115	10	-4.411138567650614
1115	11	-3.0303995926683207
1115	12	-3.383157406149234
1115	13	-2.669618780759734
1115	14	-3.46828664587105
1115	15	-3.0742607071014945
1115	16	-3.0135691711564547
1115	17	-2.9616330727370155
1115	18	-5.64280262006157
1115	19	-3.6130830993027345
1115	20	-4.117889020949097
1115	21	-3.4955418902289352
1115	22	-2.9502466987277067
1115	23	-3.5436846250477707
1115	24	-3.296613418618861
1115	25	-3.659662739484194
1116	1	-0.8713483191564464
1116	2	-0.634462898654661
1116	3	-1.1014731224504501
1116	4	-0.7525324915913956
1116	5	-0.6187383554383921
1116	6	-2.991803444962713
1116	7	-1.5940464443537827
1116	8	-2.013029111493857
1116	9	-3.0314900512994143
1116	10	-2.3199932106601864
1116	11	-1.722285758146036
1116	12	-1.4621083847481025
1116	13	-2.9609272375611475
1116	14	-6.505814845371081
1116	15	-4.1176193437699915
1116	16	-2.9054812496882505
1116	17	-3.8447499558538993
1116	18	-2.3935342827650636
1116	19	-2.1179684721458543
1116	20	-3.0175292867865444
1116	21	-3.709980034244545
1116	22	-3.2617139873076724
1116	23	-2.1600494808492456
1116	24	-3.0482977671341978
1116	25	-2.8988173642021984
1201	1	0.5708418349469042
1201	2	0.5597393413846063
1201	3	1.0412138702143992
1201	4	0.8670965138879225
1201	5	0.38677211431033953
1201	6	0.9145094626788008
1201	7	0.9696760009009893
1201	8	0.9007555464227792
1201	9	1.2971859597282318
1201	10	1.3377503964855464
1201	11	1.4093285310191845
1201	12	1.1047481791190918
1201	13	1.2488655530553814
1201	14	1.318350508023677
1201	15	1.291942713599859
1201	16	1.28372863992022
1201	17	1.5629778345133971
1201	18	1.2425523203277393
1201	19	1.472520275893821
1201	20	1.4437052271494721
1201	21	1.570812836893765
1201	22	1.392554721544741
1201	23	1.7884510885547797
1201	24	1.6954057171583017
1201	25	1.725165569014571
1202	1	1.0067567333774292
1202	2	0.9142079676787738
1202	3	1.1803657914183425
1202	4	1.2889820132359375
1202	5	1.0708448352432143
1202	6	1.3445388887434149
1202	7	1.3056237448594672
1202	8	1.2853878347701908
1202	9	1.506773896235181
1202	10	1.4483964986346223
1202	11	1.4169299616935902
1202	12	1.6837549054473513
1202	13	1.6911604228744892
1202	14	2.0599000489905634
1202	15	1.7891311260296874
1202	16	1.4538622480559482
1202	17	1.7749557631488315
1202	18	1.7901041350561329
1202	19	1.8702798788002113
1202	20	1.7079441267360735
1202	21	1.8731535866746682
1202	22	1.7369060950118822
1202	23	1.9220075950566908
1202	24	1.9764485029987142
1202	25	2.2527101504623985
1203	1	0.7937489094456041
1203	2	0.856861693152247
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2211	17	0.37287550217374843
2211	18	-0.09399606960710827
2211	19	0.2232720210599055
2211	20	-0.018949864989572518
2211	21	0.1095867487591704
2211	22	0.05740861769244532
2211	23	0.3343354760585626
2211	24	-0.056094129820870364
2211	25	0.3623345703958023
2212	1	0.16994349237455353
2212	2	0.23180491816513538
2212	3	-0.1037217554488521
2212	4	0.27745741270853586
2212	5	0.06305344215377318
2212	6	-0.09864462586093126
2212	7	-0.022551165636794934
2212	8	0.18156052916294385
2212	9	0.27475265570226526
2212	10	-0.06922508121220261
2212	11	-0.4031336973886397
2212	12	0.2713427317526964
2212	13	0.2540862831101478
2212	14	0.3208955494165906
2212	15	-0.019885699003225454
2212	16	-0.1114166053476211
2212	17	0.07788153715478074
2212	18	0.5107945224377062
2212	19	0.239328210012836
2212	20	0.27028578069733555
2212	21	0.5156479103346427
2212	22	0.45893331234147183
2212	23	0.5641367897951639
2212	24	0.5230944637566566
2212	25	0.6312760857394579
2213	1	-0.5231011192769728
2213	2	-0.4171604136435185
2213	3	-0.7513245670739899
2213	4	-0.5719457161863275
2213	5	-0.523485761302215
2213	6	-0.30476832629839734
2213	7	-0.6475613772188851
2213	8	-0.3902806070468614
2213	9	-0.778800422377877
2213	10	-0.5283985385219339
2213	11	-0.6367787566781473
2213	12	-0.6656844129699593
2213	13	-0.6548661648693397
2213	14	-0.8450483969544962
2213	15	-0.6737255796960611
2213	16	-0.5628882867534344
2213	17	-0.637275057279524
2213	18	-0.6727753937739607
2213	19	-0.7040664287011538
2213	20	-0.5022691132264043
2213	21	-0.6626309661070522
2213	22	-0.9227925786680586
2213	23	-0.7226493899228217
2213	24	-0.5236364747257554
2213	25	-0.3036041004102472
2214	1	-2.616707875667082
2214	2	-1.2211909188359158
2214	3	-1.3246543177539465
2214	4	-3.171095044146753
2214	5	-1.6104735387203384
2214	6	-1.3156534558630852
2214	7	-1.092995337187567
2214	8	-0.9370262773974964
2214	9	-0.6745145927052505
2214	10	-0.8011005325948446
2214	11	-0.6731990534837919
2214	12	-0.5969187182994418
2214	13	-1.107700727859818
2214	14	-0.9920180905671359
2214	15	-1.4472115141894974
2214	16	-1.1779515851082252
2214	17	-3.416770261687155
2214	18	-3.2258787967642526
2214	19	-1.8270336875060345
2214	20	-1.7176289473178628
2214	21	-1.6310613510093148
2214	22	-2.660500153180166
2214	23	-1.4889448061474475
2214	24	-2.8201569032019655
2214	25	-2.7207714892138775
2215	1	-1.7255441911730927
2215	2	-3.406614881091773
2215	3	-2.0259017996795343
2215	4	-3.666456059537367
2215	5	-2.087858932756525
2215	6	-1.9943955575344603
2215	7	-2.1714593573064107
2215	8	-1.3648698200046248
2215	9	-3.8912195105706253
2215	10	-2.1726075854232363
2215	11	-1.7965566162763622
2215	12	-1.7684945478043232
2215	13	-1.6818585465779954
2215	14	-1.3493182530292742
2215	15	-1.4271861347522616
2215	16	-1.5716652015137527
2215	17	-1.9739015239056705
2215	18	-1.623000166507932
2215	19	-1.8233173493379091
2215	20	-1.662480398132768
2215	21	-2.359643676779875
2215	22	-2.2178960435406445
2215	23	-2.2708534765819586
2215	24	-2.08673090561825
2215	25	-3.0951357267629227
2216	1	-3.841781219589347
2216	2	-4.070416944608706
2216	3	-5.61623669221402
2216	4	-6.008061962120866
2216	5	-5.883505255205293
2216	6	-4.7300318831911525
2216	7	-6.376034456341795
2216	8	-4.137416390881135
2216	9	-4.935307900117262
2216	10	-3.751143726810703
2216	11	-4.127795248655524
2216	12	-4.583971521611236
2216	13	-4.088816015449925
2216	14	-5.216577416133653
2216	15	-4.696175787326435
2216	16	-4.86531141951842
2216	17	-4.540387651491596
2216	18	-4.025745404976185
2216	19	-4.883797642975026
2216	20	-5.418095387352647
2216	21	-5.209793554058063
2216	22	-4.76440962500331
2216	23	-4.721514682314446
2216	24	-5.332951446787877
2216	25	-5.267693488078322
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	124020.0
3	1.0
4	1075.0
5	6610.0
6	7552.0
7	6836.0
8	5921.0
9	5598.0
10	4946.0
11	4804.0
12	4610.0
13	4482.0
14	4489.0
15	4483.0
16	4710.0
17	5142.0
18	5845.0
19	7093.0
20	9028.0
21	11541.0
22	14593.0
23	17170.0
24	20200.0
25	22769.0
26	25378.0
27	28009.0
28	31375.0
29	36544.0
30	44797.0
31	54738.0
32	67738.0
33	85128.0
34	108431.0
35	138954.0
36	184738.0
37	276055.0
38	707389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28175971817508	18.496486297567305	12.505261684295107	24.716492299962507
2	14.776924936944894	19.926417389924755	42.08463672707914	23.212020946051208
3	19.072306584390656	25.45828672135699	28.823524070894486	26.64588262335787
4	12.212224027525567	16.316161712701902	38.269712319602405	33.201901940170124
5	13.00042101480338	38.75787730166308	35.19425699489096	13.047444688642587
6	31.74023586838691	36.27178181863392	18.607857210827394	13.380125102151775
7	28.759012535062695	31.37309002158514	21.93822462503359	17.92967281831858
8	25.552798994054342	36.74203128131324	20.734141361384257	16.971028363248163
9	27.91082682540419	14.50501083844571	19.95053479707742	37.63362753907268
10	16.148626029401917	28.49126263737315	33.75209228032252	21.60801905290241
11	34.60119830120313	21.981256996825156	24.964767802242523	18.45277689972919
12	24.55870424432107	24.30158860732856	31.26403709811334	19.87567005023703
13	29.463326318084853	22.01066489922574	25.73333735024453	22.792671432444877
14	21.122131939738633	22.483909117298097	27.397420772218904	28.996538170744362
15	23.21521736739391	31.719729506005585	22.853213347571202	22.2118397790293
16	21.937572897851908	27.75706311142737	28.28281234233859	22.022551648382127
17	21.711950114474043	28.782175049871654	27.671774436154955	21.834100399499345
18	21.563869972919793	26.833844614520768	30.403103845083233	21.199181567476202
19	23.843147119997436	26.62283657260589	27.36369492762391	22.170321379772766
20	24.552101257492875	26.688653104324946	27.941507021089574	20.81773861709261
21	23.55558297193917	26.44941414216766	26.592250312857598	23.402752573035574
22	23.99519040146469	28.38509694048122	26.689493447304617	20.93021921074947
23	22.34937341848127	27.89981965126839	27.835016540336472	21.915790389913862
24	23.535803168178195	26.471561574027135	28.47697131193796	21.515663945856712
25	22.7703543799057	27.263527941078713	28.10845785943117	21.857659819584413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	208.0
1	208.0
2	513.0
3	818.0
4	818.0
5	818.0
6	1349.5
7	1881.0
8	1881.0
9	1881.0
10	2254.5
11	2628.0
12	2628.0
13	2628.0
14	4308.0
15	5988.0
16	5988.0
17	5988.0
18	12423.5
19	18859.0
20	18859.0
21	18859.0
22	32484.0
23	46109.0
24	46109.0
25	46109.0
26	70557.0
27	95005.0
28	95005.0
29	95005.0
30	125212.0
31	155419.0
32	155419.0
33	155419.0
34	179474.5
35	203530.0
36	203530.0
37	203530.0
38	218403.5
39	233277.0
40	233277.0
41	233277.0
42	247290.5
43	261304.0
44	261304.0
45	261304.0
46	273813.0
47	286322.0
48	286322.0
49	286322.0
50	272736.0
51	259150.0
52	259150.0
53	259150.0
54	227483.0
55	195816.0
56	195816.0
57	195816.0
58	168736.5
59	141657.0
60	141657.0
61	141657.0
62	117200.5
63	92744.0
64	92744.0
65	92744.0
66	71801.0
67	50858.0
68	50858.0
69	50858.0
70	37708.5
71	24559.0
72	24559.0
73	24559.0
74	17582.0
75	10605.0
76	10605.0
77	10605.0
78	7377.0
79	4149.0
80	4149.0
81	4149.0
82	2773.5
83	1398.0
84	1398.0
85	1398.0
86	901.0
87	404.0
88	404.0
89	404.0
90	241.0
91	78.0
92	78.0
93	78.0
94	50.0
95	22.0
96	22.0
97	22.0
98	13.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3324745125172497
2	0.008505384194893712
3	0.007693072221224086
4	0.009174346996739285
5	0.010942320115902585
6	0.0736336912602877
7	0.07349034208846364
8	0.09666512486668527
9	0.07764746807136114
10	0.08467157749073964
11	0.07955879036234847
12	0.09097894105099791
13	0.07802973252955861
14	0.0812789804242371
15	0.07908095978960164
16	0.08032331927874342
17	0.09198238525376626
18	0.09394149060202829
19	0.08787304232814346
20	0.08629620143807888
21	0.07573614578037377
22	0.09427597200295108
23	0.08806417455724219
24	0.08352478411614724
25	0.08271247214247761
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2092792.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.07087889548129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.89939569994769	44.656154948273574
2	13.234811892961806	15.371143172813213
3	4.488472609018941	7.819486479120716
4	1.9410625396137033	4.508768306658509
5	1.0341444421577646	3.0026838330489305
6	0.6307664301512469	2.197749658598887
7	0.3975724707842789	1.616116796216379
8	0.26549666991581194	1.233409997266775
9	0.17733375295975506	0.9268134202986419
>10	0.84651264324627	8.413491213801692
>50	0.045637515387758155	1.8328440835541935
>100	0.032592255285950666	3.853707247330736
>500	0.0037206471414610986	1.560442357966596
>1k	0.0023150693324646638	2.4512868967269568
>5k	1.6536209517604742E-4	0.5559015883242195
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	6157	0.294200283640228	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5421	0.25903195348606073	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4746	0.22677838982564918	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4354	0.20804743137397314	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3788	0.18100222095650212	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2786	0.133123597567269	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2266	0.10827640778443343	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2218	0.10598282103524861	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2204	0.10531385823340303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7783057274683775E-5
2	0.0	0.0	0.0	0.0	4.7783057274683775E-5
3	0.0	0.0	0.0	0.0	4.7783057274683775E-5
4	0.0	0.0	0.0	0.0	4.7783057274683775E-5
5	0.0	0.0	0.0	0.0	4.7783057274683775E-5
6	0.0	0.0	0.0	0.0	4.7783057274683775E-5
7	0.0	0.0	0.0	0.0	4.7783057274683775E-5
8	0.0	0.0	0.0	4.7783057274683775E-5	4.7783057274683775E-5
9	0.0	0.0	0.0	4.7783057274683775E-5	4.7783057274683775E-5
10	0.0	0.0	0.0	9.556611454936755E-5	4.7783057274683775E-5
11	4.7783057274683775E-5	0.0	0.0	9.556611454936755E-5	4.7783057274683775E-5
12	4.7783057274683775E-5	0.0	0.0	9.556611454936755E-5	4.7783057274683775E-5
13	4.7783057274683775E-5	0.0	0.0	9.556611454936755E-5	4.7783057274683775E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	840	0.0	12.438395	4
GGTATCA	1580	0.0	12.298471	1
TGCGTAT	55	0.003074006	12.088306	10
CAAGTCG	195	0.0	11.69119	6
GCGCCAC	155	2.5465852E-11	11.64349	13
AAGTCGT	215	0.0	11.487273	7
CGTCTAG	60	0.0057616606	11.112834	1
TCCAACG	60	0.0058820406	11.082008	18
GTCCTAA	1500	0.0	10.922329	1
TGTAGGA	3075	0.0	10.811198	2
TTAGGAC	1485	0.0	10.809638	3
TGCGCCA	185	5.456968E-12	10.782236	12
GACGTGA	1405	0.0	10.749861	7
AGGACCT	1680	0.0	10.7422495	5
CTGTAGG	3010	0.0	10.6012335	1
AGGACGT	2940	0.0	10.53225	5
GTCCTAC	2870	0.0	10.487772	1
CCGTACA	145	1.4466423E-8	10.481506	17
GGACGTG	2855	0.0	10.480609	6
CACCTTT	775	0.0	10.41786	14
>>END_MODULE