FastQCFastQC Report
Thu 26 May 2016
SRR1512838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092792
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT59680.28516928581531276No Hit
CTGTAGGACGTGGAATATGGCAAGA49970.23877193720159479No Hit
GTCCTAAAGTGTGTATTTCTCATTT48120.2299320716057783No Hit
CTTTAGGACGTGAAATATGGCGAGG48030.22950202409030615No Hit
GTATCAACGCAGAGTACTTTTTTTT45740.21855970397440358No Hit
TATCAACGCAGAGTACTTTTTTTTT34270.16375253728034128No Hit
GGTATCAACGCAGAGTACTTTTTTT27070.12934873604256897No Hit
GTCCTACAGTGTGCATTTCTCATTT26770.12791524432432844No Hit
CTGAAGGACCTGGAATATGGCGAGA24600.11754632089572208No Hit
CTGTAGGACCTGGAATATGGCGAGA21310.10182569505235112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9900.013.0652064
GGTATCA13600.012.2397931
CGTGCGC1251.4224497E-912.15420210
AACAGCG550.003056954812.0972887
GCGTGCG1501.7644197E-1011.3967459
GTATCAA30800.011.2724181
CCCCGTA1701.2732926E-1111.16793915
TACGGAG600.005827767311.0958185
GACCGTC600.00585378111.0891817
AGGACCT18850.011.0495685
CCCGTAC1752.0008883E-1110.84885416
GGACCTG17250.010.8063616
TAGAAAT6900.010.7512274
CCCGCCC3200.010.67934114
AATCCCG909.596387E-510.54699319
ATAGGAC3550.010.4501273
AATGTCC6650.010.4288348
GGCGTGC2100.010.4050248
GGCGAGG13150.010.39460419
GTATAAT3400.010.3513681
Produced by FastQC (version 0.11.5)