##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512838_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2092792 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.53807210654475 31.0 31.0 34.0 27.0 34.0 2 30.530498969797286 31.0 31.0 34.0 27.0 34.0 3 30.722288215933546 31.0 31.0 34.0 27.0 34.0 4 34.35612330322363 37.0 35.0 37.0 31.0 37.0 5 34.0003454715041 37.0 35.0 37.0 30.0 37.0 6 33.917351079323694 37.0 35.0 37.0 30.0 37.0 7 33.76409552406546 37.0 35.0 37.0 29.0 37.0 8 33.71724089159362 37.0 35.0 37.0 28.0 37.0 9 35.09969695985076 39.0 35.0 39.0 28.0 39.0 10 35.0045690159366 39.0 35.0 39.0 28.0 39.0 11 35.01832002415912 39.0 35.0 39.0 28.0 39.0 12 34.90483382964002 39.0 35.0 39.0 27.0 39.0 13 34.89905112404864 39.0 35.0 39.0 27.0 39.0 14 35.99997180799621 40.0 36.0 41.0 27.0 41.0 15 36.01171640564375 40.0 36.0 41.0 27.0 41.0 16 36.007801061930664 40.0 36.0 41.0 27.0 41.0 17 35.988216698076066 40.0 36.0 41.0 27.0 41.0 18 35.914131934755105 40.0 36.0 41.0 27.0 41.0 19 35.92035424447341 40.0 36.0 41.0 27.0 41.0 20 35.9024145734502 40.0 36.0 41.0 27.0 41.0 21 35.86704794360835 40.0 36.0 41.0 27.0 41.0 22 35.82807942690913 40.0 36.0 41.0 27.0 41.0 23 35.68209788645981 40.0 36.0 41.0 27.0 41.0 24 35.657181411243926 40.0 36.0 41.0 27.0 41.0 25 35.58971746833895 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6936058403625083 1101 2 0.6012322958008092 1101 3 0.5412661341779206 1101 4 0.6384938832887457 1101 5 0.6227487144565416 1101 6 0.7448682667977309 1101 7 0.8024007262993678 1101 8 0.5602431855461987 1101 9 0.9626130499009804 1101 10 0.5145911613812686 1101 11 0.8750553882338892 1101 12 1.0480305206796885 1101 13 1.121433512701742 1101 14 1.0537521807558363 1101 15 0.8099783657432411 1101 16 1.028340107204464 1101 17 1.1489417007078444 1101 18 1.0498186679007517 1101 19 0.8560963268025645 1101 20 1.0098373947772004 1101 21 1.0822691515834535 1101 22 1.0858460154688103 1101 23 1.1019307769610407 1101 24 1.1561038348989143 1101 25 1.2339658743920197 1102 1 0.8777242548017519 1102 2 0.9931226461083114 1102 3 0.5512157029998193 1102 4 0.7309789035911081 1102 5 0.9118757976369238 1102 6 0.92157053083573 1102 7 0.9241414772890764 1102 8 0.9340155845003011 1102 9 1.1192128703140298 1102 10 1.0604349659138634 1102 11 1.119002235014598 1102 12 1.1194112506611091 1102 13 1.1467789916849895 1102 14 1.1604528070512075 1102 15 1.2901351372442562 1102 16 1.3301399298764736 1102 17 1.4416729471429335 1102 18 0.8064263946575139 1102 19 1.1116835704817234 1102 20 1.2702825219281522 1102 21 1.1310275732513588 1102 22 1.0102082841376614 1102 23 1.1944090203973659 1102 24 1.1502825069720117 1102 25 1.2807962776720245 1103 1 0.7891349807304628 1103 2 0.9359578854346715 1103 3 0.6337319412711295 1103 4 0.5546140848119379 1103 5 0.7171195700798734 1103 6 0.725970597505949 1103 7 0.8041976347752211 1103 8 0.7992313201839423 1103 9 0.8199716429270083 1103 10 0.6682095198048543 1103 11 0.8092525548259388 1103 12 1.0219217539499112 1103 13 1.0231284024218326 1103 14 1.1303740413067729 1103 15 1.0412155684847164 1103 16 1.0582049442580086 1103 17 1.0109086704581571 1103 18 0.8782703594700791 1103 19 1.038117683467597 1103 20 0.8462759523083605 1103 21 0.9988732560396514 1103 22 0.9445542379311718 1103 23 1.1587612759542338 1103 24 1.0587364001896589 1103 25 1.021204603388803 1104 1 0.7793776638808616 1104 2 0.8837061253062508 1104 3 0.8149002949215145 1104 4 0.5650595205205349 1104 5 0.7622903438163107 1104 6 0.6871535428516466 1104 7 0.6260419737478315 1104 8 0.6304519200491185 1104 9 0.6802561189109255 1104 10 0.8470785434069015 1104 11 0.925196030422093 1104 12 0.7901060645254105 1104 13 0.8086529937322879 1104 14 0.9990528390272218 1104 15 0.9895689126356046 1104 16 0.9834074009267582 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2216 17 -2.9399401567343233 2216 18 -3.470737196468342 2216 19 -3.739554248303264 2216 20 -2.9002254606553066 2216 21 -3.624311427370664 2216 22 -3.0683900986116655 2216 23 -4.466101035584387 2216 24 -3.453980399932405 2216 25 -3.7133484455293058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44121.0 3 1.0 4 751.0 5 6200.0 6 7454.0 7 5821.0 8 4742.0 9 4230.0 10 3514.0 11 3374.0 12 3123.0 13 2962.0 14 2946.0 15 3017.0 16 3091.0 17 3324.0 18 3673.0 19 4428.0 20 5743.0 21 7563.0 22 9945.0 23 12056.0 24 14628.0 25 16966.0 26 19288.0 27 21330.0 28 24639.0 29 29514.0 30 37257.0 31 47432.0 32 61056.0 33 78135.0 34 101529.0 35 133678.0 36 182512.0 37 280562.0 38 902187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.267099366492644 19.541743720998518 13.419576797763044 26.771580114745795 2 13.483910938420236 20.492758583764846 40.8625641535555 25.160766324259416 3 17.308986358353216 25.5761282651251 29.626900195754573 27.48798518076711 4 11.666042265581993 16.999176172854607 37.45111512936571 33.88366643219769 5 12.589474026306094 39.06719041162473 35.228380107313875 13.114955454755309 6 31.782521367909577 36.87376617862841 18.15888631081613 13.184826142645885 7 28.516064364579584 31.55888226607393 21.97492405867185 17.950129310674633 8 25.605681587815226 37.20131884440466 20.280675181015983 16.912324386764126 9 27.356313598409354 14.434407307036034 19.88648475550011 38.3227943390545 10 15.768468799241209 28.902963268927746 33.72420334419508 21.604364587635963 11 34.4376769265763 21.9878758106845 24.976059361315368 18.598387901423823 12 24.15139273942532 24.589422118500636 31.269047612221435 19.990137529852607 13 29.3419443010876 21.90893973278284 25.792094347917505 22.957021618212053 14 20.918108926881946 22.485019925649137 27.371486730568904 29.225384416900006 15 23.054182813411728 31.80757046384587 22.808642373551212 22.329604349191193 16 21.999991398338636 27.80338895900305 28.02574190523932 22.170877737418994 17 21.472670260744245 29.105864093406513 27.647701392744885 21.77376425310436 18 21.43490300738047 27.012187691888652 30.416140030630505 21.136769270100373 19 23.784416854098485 26.62071779816233 27.44087834205626 22.153987005682925 20 24.474851152078138 26.7290251058421 27.931897894626186 20.864225847453575 21 23.329512272390836 26.314383642482486 26.820025162618077 23.536078922508597 22 23.930884311354124 28.262680137099867 26.69476643757756 21.11166911396845 23 21.987373804945737 27.928384665078994 27.985533201579514 22.098708328395748 24 23.563020118578436 26.627682063004826 28.32770767472353 21.48159014369321 25 22.55603996957175 27.417918264213547 28.174658542272713 21.851383223941987 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 68.0 1 68.0 2 209.0 3 350.0 4 350.0 5 350.0 6 898.5 7 1447.0 8 1447.0 9 1447.0 10 1877.5 11 2308.0 12 2308.0 13 2308.0 14 4008.5 15 5709.0 16 5709.0 17 5709.0 18 12286.5 19 18864.0 20 18864.0 21 18864.0 22 33128.5 23 47393.0 24 47393.0 25 47393.0 26 72914.5 27 98436.0 28 98436.0 29 98436.0 30 127777.5 31 157119.0 32 157119.0 33 157119.0 34 180874.5 35 204630.0 36 204630.0 37 204630.0 38 219929.0 39 235228.0 40 235228.0 41 235228.0 42 249015.0 43 262802.0 44 262802.0 45 262802.0 46 275326.5 47 287851.0 48 287851.0 49 287851.0 50 273532.5 51 259214.0 52 259214.0 53 259214.0 54 226507.0 55 193800.0 56 193800.0 57 193800.0 58 166601.0 59 139402.0 60 139402.0 61 139402.0 62 114892.0 63 90382.0 64 90382.0 65 90382.0 66 69529.0 67 48676.0 68 48676.0 69 48676.0 70 36101.5 71 23527.0 72 23527.0 73 23527.0 74 16751.0 75 9975.0 76 9975.0 77 9975.0 78 6958.0 79 3941.0 80 3941.0 81 3941.0 82 2602.5 83 1264.0 84 1264.0 85 1264.0 86 802.5 87 341.0 88 341.0 89 341.0 90 199.0 91 57.0 92 57.0 93 57.0 94 32.0 95 7.0 96 7.0 97 7.0 98 4.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08290360437157634 2 0.04472494160910401 3 0.0179664295352811 4 0.0057339668729620525 5 0.020307799341740602 6 0.058343112932388884 7 0.08142232959606115 8 0.06039778439520029 9 0.02685407818837228 10 0.05007664402386859 11 0.005542834643863318 12 4.778305727468377E-4 13 0.005351702414764583 14 5.733966872962053E-4 15 0.0126625101777912 16 0.008266468908520293 17 0.004300475154721539 18 0.005112787128391164 19 0.001481274775515197 20 0.002484718978283556 21 2.3891528637341885E-4 22 5.256136300215215E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2092792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.17493106251078 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29265562454161 40.99092152534938 2 14.593178661032269 16.10355253210718 3 5.01613535975138 8.302947680235153 4 2.231297829333374 4.924468156535955 5 1.1773760890613953 3.2480822274305527 6 0.6912951550938994 2.2885297515692162 7 0.4296693025064077 1.6594881901827698 8 0.3094934462544742 1.36610236491116 9 0.20907098546693778 1.0381929489278539 >10 0.9543244535295412 9.075277118758057 >50 0.052608822376912334 2.0296414598499806 >100 0.035964410742736076 4.026036067339397 >500 0.0038980464238908196 1.5199549838388138 >1k 0.0029451906313841627 3.1415684876016843 >5k 8.662325386424007E-5 0.2852365053628707 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5968 0.28516928581531276 No Hit CTGTAGGACGTGGAATATGGCAAGA 4997 0.23877193720159479 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4812 0.2299320716057783 No Hit CTTTAGGACGTGAAATATGGCGAGG 4803 0.22950202409030615 No Hit GTATCAACGCAGAGTACTTTTTTTT 4574 0.21855970397440358 No Hit TATCAACGCAGAGTACTTTTTTTTT 3427 0.16375253728034128 No Hit GGTATCAACGCAGAGTACTTTTTTT 2707 0.12934873604256897 No Hit GTCCTACAGTGTGCATTTCTCATTT 2677 0.12791524432432844 No Hit CTGAAGGACCTGGAATATGGCGAGA 2460 0.11754632089572208 No Hit CTGTAGGACCTGGAATATGGCGAGA 2131 0.10182569505235112 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.7783057274683775E-5 0.0 5 0.0 0.0 0.0 4.7783057274683775E-5 0.0 6 0.0 0.0 0.0 4.7783057274683775E-5 0.0 7 0.0 0.0 0.0 4.7783057274683775E-5 0.0 8 0.0 0.0 0.0 4.7783057274683775E-5 0.0 9 0.0 0.0 0.0 4.7783057274683775E-5 0.0 10 0.0 0.0 0.0 1.4334917182405132E-4 0.0 11 0.0 0.0 0.0 1.4334917182405132E-4 0.0 12 0.0 0.0 0.0 1.911322290987351E-4 0.0 13 0.0 0.0 0.0 1.911322290987351E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 990 0.0 13.065206 4 GGTATCA 1360 0.0 12.239793 1 CGTGCGC 125 1.4224497E-9 12.154202 10 AACAGCG 55 0.0030569548 12.097288 7 GCGTGCG 150 1.7644197E-10 11.396745 9 GTATCAA 3080 0.0 11.272418 1 CCCCGTA 170 1.2732926E-11 11.167939 15 TACGGAG 60 0.0058277673 11.095818 5 GACCGTC 60 0.005853781 11.089181 7 AGGACCT 1885 0.0 11.049568 5 CCCGTAC 175 2.0008883E-11 10.848854 16 GGACCTG 1725 0.0 10.806361 6 TAGAAAT 690 0.0 10.751227 4 CCCGCCC 320 0.0 10.679341 14 AATCCCG 90 9.596387E-5 10.546993 19 ATAGGAC 355 0.0 10.450127 3 AATGTCC 665 0.0 10.428834 8 GGCGTGC 210 0.0 10.405024 8 GGCGAGG 1315 0.0 10.394604 19 GTATAAT 340 0.0 10.351368 1 >>END_MODULE