FastQCFastQC Report
Thu 26 May 2016
SRR1512837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494563
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51290.3431772364229544No Hit
TATCAACGCAGAGTACTTTTTTTTT29880.1999246602518596No Hit
GGTATCAACGCAGAGTACTTTTTTT29610.1981181121170536No Hit
GTACTGGTTCACTATCGGTCAGTCA24650.16493115378876635No Hit
GGATACCACGTGTCCCGCCCTACTC15250.10203651502144774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTT350.002171602816.28407912
GTCCGAC456.767261E-414.77629312
GACGGAA400.00528366814.2480917
GGTATCA13400.012.7214111
GTATTGG1405.0931703E-1112.244231
GACGGTG550.003071428512.0892897
AACCGTG658.032119E-411.6907427
TAGGACC2450.011.6310944
TAGGACA3200.011.2797384
GTATAAT2300.011.1795151
GTATCAA30750.011.0872771
AAGGTTA1053.473664E-610.85496119
GTCCTAC6000.010.7930631
CTTACAC1502.1354936E-910.7652243
TAAAGGT2150.010.603234
GTGTAGC3150.010.5814341
AAGTGTA1355.6954377E-810.5541417
GTCCTAT1105.8694695E-610.3890451
CAAGAGT1409.701398E-810.1772074
GTATAGT1605.9244485E-910.1184971
Produced by FastQC (version 0.11.5)