FastQCFastQC Report
Thu 26 May 2016
SRR1512837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494563
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68290.4569228597255519No Hit
TATCAACGCAGAGTACTTTTTTTTT50580.3384266839203165No Hit
GGTATCAACGCAGAGTACTTTTTTT40850.27332404187712395No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23340.15616604987544855No Hit
GTACTGGTTCACTATCGGTCAGTCA21960.14693258163088474No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16380.10959725351156159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1600.014.262923
TAGGACC2300.013.6437084
TAAGACC905.341062E-712.6790014
GGTATCA14000.012.2982241
GGTTATA1251.380613E-912.1759221
TCGTCTC550.003082960112.08324216
TAGACAG2000.011.8865645
TAAAACG1052.741872E-711.75444812
GTATACG657.935498E-411.7076191
GTTTATA1251.7958882E-811.41492751
GTAGAGC951.346391E-511.0099743
GTTCTAA1053.4113982E-610.8713591
GTATCAA30200.010.83536151
TAGGACT2200.010.8059664
GTCCTAT1252.1991036E-710.6539331
GTCCTAG1252.1991036E-710.6539331
CCCCGTA1909.094947E-1210.49369415
CCCGTAC1909.094947E-1210.49334216
GTCCTAC7650.010.445031
GGCGAGG3000.010.441627519
Produced by FastQC (version 0.11.5)