##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512837_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1494563
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.045136270602175	31.0	30.0	34.0	26.0	34.0
2	30.066076170760283	31.0	30.0	34.0	26.0	34.0
3	30.260713666804275	31.0	30.0	34.0	26.0	34.0
4	33.86775130924558	37.0	35.0	37.0	30.0	37.0
5	33.413013034579336	37.0	35.0	37.0	28.0	37.0
6	33.25939689394157	37.0	35.0	37.0	27.0	37.0
7	33.104997915778725	37.0	35.0	37.0	26.0	37.0
8	33.03453785487798	37.0	35.0	37.0	26.0	37.0
9	34.336380600884674	39.0	35.0	39.0	25.0	39.0
10	34.27687156714036	39.0	35.0	39.0	25.0	39.0
11	34.247640949227296	39.0	35.0	39.0	25.0	39.0
12	34.10347974625359	39.0	35.0	39.0	25.0	39.0
13	34.080185311693114	39.0	35.0	39.0	25.0	39.0
14	35.14770939732885	39.0	35.0	41.0	25.0	41.0
15	35.12567218645182	39.0	35.0	41.0	25.0	41.0
16	35.12658482780586	39.0	35.0	41.0	25.0	41.0
17	35.117754152886164	39.0	35.0	41.0	25.0	41.0
18	35.04126891940989	39.0	35.0	41.0	24.0	41.0
19	35.04864967217842	40.0	35.0	41.0	24.0	41.0
20	35.02201579993617	40.0	35.0	41.0	24.0	41.0
21	34.986339150641356	40.0	35.0	41.0	24.0	41.0
22	34.94503677663638	40.0	35.0	41.0	23.0	41.0
23	34.78211691310437	39.0	35.0	41.0	22.0	41.0
24	34.724060477878815	39.0	35.0	41.0	21.0	41.0
25	34.666252944840735	39.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4925126915578808
1101	2	0.33329550533866836
1101	3	0.4139354625930274
1101	4	0.25981900265750824
1101	5	0.38038987851840034
1101	6	0.48838917502759926
1101	7	0.6093842820099908
1101	8	0.3471808963490872
1101	9	0.5936082594009662
1101	10	0.24616118813730026
1101	11	0.6834341946411655
1101	12	0.6745957583517637
1101	13	0.8426396678788706
1101	14	0.9117941072482623
1101	15	0.6341585001932586
1101	16	0.7789437109122659
1101	17	0.9752941632294849
1101	18	0.8258336954087397
1101	19	0.6702999787965922
1101	20	0.6523755345317994
1101	21	0.6520974546071372
1101	22	0.6723759428584231
1101	23	0.8413396078697986
1101	24	0.788050864752897
1101	25	0.9077820892396176
1102	1	0.7823163546813028
1102	2	0.9747229692570087
1102	3	0.6104316203164188
1102	4	0.8085741804855715
1102	5	1.0899433749777288
1102	6	0.7976131347988229
1102	7	0.8141379097047903
1102	8	0.8868943922444501
1102	9	1.188418170879892
1102	10	0.9963481434305521
1102	11	1.1333032163824726
1102	12	1.1239534037801207
1102	13	1.1141271423029124
1102	14	1.0492327787672906
1102	15	1.1866634534368146
1102	16	1.0842988154633844
1102	17	1.1710638388808619
1102	18	0.7159116040853917
1102	19	0.999736258812085
1102	20	1.2083606665131867
1102	21	1.0093702108054359
1102	22	0.8962665525298803
1102	23	1.1747020919393023
1102	24	1.2784536993476152
1102	25	1.1276849544140362
1103	1	0.8011481235895843
1103	2	0.9329646100554534
1103	3	0.6872844447828079
1103	4	0.6229070269095942
1103	5	0.7326193882106011
1103	6	0.6926796933962862
1103	7	0.7979314526819223
1103	8	0.8625427607619329
1103	9	0.8500483342953302
1103	10	0.7821265425190589
1103	11	1.0095667796842278
1103	12	1.0004499586071205
1103	13	1.0384945805881856
1103	14	1.1542938330100299
1103	15	1.0605154516894686
1103	16	1.202266890206019
1103	17	1.0953709273163739
1103	18	1.1868207344010813
1103	19	1.038023100340105
1103	20	0.971044504673948
1103	21	1.0196556562527164
1103	22	0.971661264836726
1103	23	1.0012282237979377
1103	24	1.0576932015566456
1103	25	0.9718371660772149
1104	1	0.8240704909592793
1104	2	0.9320816841930153
1104	3	0.825206989836758
1104	4	0.7515372352104848
1104	5	0.8891894373870102
1104	6	0.8560629260846184
1104	7	0.7584919603972651
1104	8	0.7021900520296498
1104	9	0.8160923245193956
1104	10	0.7967767005481363
1104	11	0.9530509150451891
1104	12	0.9797118252589385
1104	13	0.9599323136155817
1104	14	1.1419658432710236
1104	15	1.134045957144373
1104	16	1.0722342585850342
1104	17	1.0633375710367403
1104	18	0.7196677746394187
1104	19	1.1213474884010424
1104	20	1.096287721440305
1104	21	1.0962300577773618
1104	22	0.9986088216050106
1104	23	0.9790990242673132
1104	24	1.1920030777601838
1104	25	1.013614396921298
1105	1	0.4977392369788234
1105	2	0.49284033221511336
1105	3	0.5003724684084752
1105	4	0.27458180790588926
1105	5	0.3512255780110394
1105	6	0.6649633403120703
1105	7	0.6664574276404167
1105	8	0.5335618889722014
1105	9	0.8519828956241469
1105	10	0.5911611392617786
1105	11	0.8157494539294987
1105	12	0.8193434748966126
1105	13	0.7094149550133437
1105	14	0.8590312293321247
1105	15	0.9546072623238402
1105	16	0.8512138239733389
1105	17	0.7467863439759554
1105	18	0.8001426923594579
1105	19	0.887867427477758
1105	20	0.952478431337866
1105	21	0.7783095444930979
1105	22	0.7646885241370356
1105	23	0.852244935615218
1105	24	0.8297846584137218
1105	25	0.5659155201518544
1106	1	0.2334245685013805
1106	2	0.4688559549154192
1106	3	-1.5047356982407294
1106	4	-0.158396701739548
1106	5	0.11254224503422705
1106	6	-0.1072108547709334
1106	7	0.24351644077823664
1106	8	0.3245302027616219
1106	9	0.5678184316360841
1106	10	0.3418500991019968
1106	11	0.5687660586249734
1106	12	0.7215196793125003
1106	13	0.6799680810252084
1106	14	0.6763514228350829
1106	15	0.7364244269265967
1106	16	0.7148942087706445
1106	17	0.524823073996032
1106	18	0.6528955241201544
1106	19	0.5890452549007463
1106	20	0.7581216400029689
1106	21	0.5547852477458974
1106	22	0.6407254834294847
1106	23	0.6575356425694636
1106	24	0.6991403573224133
1106	25	0.7465463190381101
1107	1	0.6167331040073947
1107	2	0.6213856942602938
1107	3	0.623642328935091
1107	4	0.4412782915105353
1107	5	0.5508153272272978
1107	6	0.47332667693924435
1107	7	0.4659569223830857
1107	8	0.48814550941003887
1107	9	0.534295884022093
1107	10	0.5511006271838212
1107	11	0.707227272224145
1107	12	0.8354021672095726
1107	13	0.6721240987034918
1107	14	0.6609072854457381
1107	15	0.7194546997676738
1107	16	0.8201813798835929
1107	17	0.7007548153593817
1107	18	0.6452439325111357
1107	19	0.6474423334495754
1107	20	0.6873187895883746
1107	21	0.6184543010680841
1107	22	0.586638426090559
1107	23	0.8417033668436176
1107	24	0.7638803735799655
1107	25	0.874882475064453
1108	1	0.45417465365824583
1108	2	0.3365938362126215
1108	3	0.3711302589811609
1108	4	0.2625013801754861
1108	5	0.46389594554047164
1108	6	0.4825891146900574
1108	7	0.3157693977769611
1108	8	0.3586822165746497
1108	9	0.5624553110187307
1108	10	0.6665009559562449
1108	11	0.6248568299699784
1108	12	0.7118623035534313
1108	13	0.8045085275995518
1108	14	0.9397057811046992
1108	15	0.7633668843775325
1108	16	0.7328135622832121
1108	17	0.7783250766505745
1108	18	0.8274259867862099
1108	19	0.838783911419064
1108	20	0.6736624741878714
1108	21	0.8100702589631084
1108	22	0.685460440028848
1108	23	0.81007801545082
1108	24	0.8900820160606813
1108	25	0.8984591184085389
1109	1	0.4598196376546042
1109	2	0.4378516238151242
1109	3	0.6192363936817991
1109	4	0.490854917854584
1109	5	0.2060683555729952
1109	6	0.5743059964985306
1109	7	0.5844406886797202
1109	8	0.6710270920335972
1109	9	0.8771130546523267
1109	10	0.5465525761410888
1109	11	0.7054779686260915
1109	12	0.9044168449006449
1109	13	0.7565408776335687
1109	14	0.9619150973190074
1109	15	1.0118017472170564
1109	16	1.066126757628048
1109	17	1.120035813198946
1109	18	1.269013991238495
1109	19	1.1192795482843891
1109	20	1.2224200064591457
1109	21	1.2032472891925963
1109	22	1.1096750152009633
1109	23	1.2388232918230742
1109	24	1.239329957398546
1109	25	1.1960330389633071
1110	1	0.2534399804465082
1110	2	0.14678961613317298
1110	3	0.21664826688448713
1110	4	0.2842606771641414
1110	5	0.14208428336183943
1110	6	0.2896682988334618
1110	7	0.2787161708154713
1110	8	0.17391251070402802
1110	9	0.07102568931387765
1110	10	0.21392769237380804
1110	11	0.292991918288557
1110	12	0.449923473724688
1110	13	0.42231113164069
1110	14	0.35580634307682146
1110	15	0.37737630664383204
1110	16	0.1653028681670179
1110	17	0.24517583065729553
1110	18	0.1478125181819152
1110	19	0.30809912385353755
1110	20	0.30666346773932673
1110	21	0.3282630010090628
1110	22	0.33582795002939037
1110	23	0.3752605393489432
1110	24	0.38733735184812446
1110	25	0.3297084790157001
1111	1	0.013501563891249901
1111	2	-0.1351370443320974
1111	3	-0.10908274454321187
1111	4	0.14434932874501527
1111	5	0.2230863677457222
1111	6	0.33116258360949047
1111	7	0.10105026856030008
1111	8	0.29912634210938194
1111	9	0.24345727356929103
1111	10	0.12115062526818576
1111	11	0.11442363032755765
1111	12	0.2640495370421476
1111	13	0.22076618685556326
1111	14	-0.1001041974202721
1111	15	0.07761284368115184
1111	16	-0.03369121394149488
1111	17	0.14431198613007012
1111	18	-2.7457526361018836E-4
1111	19	-0.08588137609631019
1111	20	0.21722938414687576
1111	21	0.21884864830163053
1111	22	0.00792633514713259
1111	23	-0.06683872513972489
1111	24	0.11886403594648698
1111	25	0.06996257599107736
1112	1	0.2371802013696751
1112	2	-0.1325891270879893
1112	3	0.25064152672772266
1112	4	0.29689941457351665
1112	5	0.24119591107572802
1112	6	0.1888961621735774
1112	7	0.3570336978647859
1112	8	0.34681300470543874
1112	9	0.25174567089732136
1112	10	-0.15620352557176886
1112	11	0.10203338949948204
1112	12	0.4474421450156214
1112	13	0.3634932630548775
1112	14	0.06228685662694744
1112	15	-0.05142182452882338
1112	16	-0.049453772925296846
1112	17	0.0954073584387416
1112	18	0.2304077427091471
1112	19	0.09635781605179261
1112	20	0.2802708102079734
1112	21	0.014555122271502796
1112	22	0.2514759825149824
1112	23	0.5126695415717606
1112	24	0.3243021679278897
1112	25	0.2450810179716143
1113	1	-0.566840621572883
1113	2	-0.4566467776013461
1113	3	-0.5161124513644175
1113	4	-1.9847342131815253
1113	5	-0.8402021076300912
1113	6	-0.4890198856710555
1113	7	-0.33733648408103534
1113	8	-0.37201917491932335
1113	9	-0.23312356660061795
1113	10	-0.21219891397465318
1113	11	-0.42523940714143293
1113	12	-0.12259128351398374
1113	13	-0.3191850842896784
1113	14	-0.41281759452154176
1113	15	-0.6383422446503175
1113	16	-0.7192095205999038
1113	17	-0.9583186497064702
1113	18	-0.635205440853646
1113	19	-0.33986038990651934
1113	20	-0.07285037670946082
1113	21	-0.09021494646543715
1113	22	-0.18064516486258952
1113	23	-0.11854437396414852
1113	24	0.09574352787733176
1113	25	-0.13716561645734515
1114	1	-0.719015019531426
1114	2	-2.1379350883163006
1114	3	-1.3510510798668456
1114	4	-0.7268854425446705
1114	5	-0.5426232109786824
1114	6	-0.3122901968559191
1114	7	-0.311400446059551
1114	8	-0.39343748554505
1114	9	-0.802523770627019
1114	10	-0.77719292107097
1114	11	-0.7322389104590954
1114	12	-0.7233679816768586
1114	13	-0.8387757823514264
1114	14	-0.7936116022126853
1114	15	-0.9429155885716085
1114	16	-1.1055599760903263
1114	17	-1.2254787271532308
1114	18	-0.8722902129299897
1114	19	-1.3108602382250893
1114	20	-0.8132810627556637
1114	21	-1.0390864068602212
1114	22	-0.8420252874568774
1114	23	-0.6553947833553124
1114	24	-0.6643834384047764
1114	25	-1.263821843872421
1115	1	-0.9558712247420615
1115	2	-1.0886182307715977
1115	3	-0.5306583845796347
1115	4	-0.42657289370891505
1115	5	-0.809741957211358
1115	6	-0.5592339157405704
1115	7	-0.4560737209016068
1115	8	-0.6997096709311634
1115	9	-1.7055789354965327
1115	10	-1.3243654703532215
1115	11	-1.7633542633044001
1115	12	-2.5078197933438595
1115	13	-2.437494332124828
1115	14	-1.8033128948253676
1115	15	-2.069977852764673
1115	16	-1.8213861096379773
1115	17	-1.5906451594082682
1115	18	-2.085470843832134
1115	19	-2.637549676306932
1115	20	-2.743349377001195
1115	21	-1.8388196241039907
1115	22	-1.7787140557912124
1115	23	-1.7601589206089017
1115	24	-2.3740144075294154
1115	25	-2.121929137422754
1116	1	-1.287029153289506
1116	2	-1.5505785770843588
1116	3	-1.4993373006732504
1116	4	-1.103562148085274
1116	5	-1.7568471358427473
1116	6	-2.364113976476702
1116	7	-1.9317212323261295
1116	8	-2.333002854176165
1116	9	-3.365293431868423
1116	10	-2.0154178406684338
1116	11	-3.069255961288139
1116	12	-5.027326166257879
1116	13	-4.088989375413924
1116	14	-3.553914416148718
1116	15	-4.666582615295152
1116	16	-4.153359814980128
1116	17	-3.8166295665739653
1116	18	-3.700028170858257
1116	19	-3.9504608497115115
1116	20	-6.102036761113439
1116	21	-4.792547377305045
1116	22	-4.020047476724741
1116	23	-4.620618018618913
1116	24	-4.458663568757569
1116	25	-3.831666628845234
1201	1	0.6578001833995515
1201	2	0.8128659273322612
1201	3	0.9112735567028949
1201	4	0.7909647835836537
1201	5	0.4036712148782655
1201	6	0.812705614139297
1201	7	1.04967403561443
1201	8	0.9566847944269838
1201	9	0.995909697455879
1201	10	1.049403713229303
1201	11	0.7289340810202773
1201	12	1.2594058564880584
1201	13	1.3081176127375826
1201	14	1.2947274944118732
1201	15	1.1627124075409938
1201	16	1.2219574599114225
1201	17	1.0682153204073686
1201	18	1.2850195809242138
1201	19	1.440724374605658
1201	20	1.5146426092908527
1201	21	1.123871139622139
1201	22	1.1114148012019456
1201	23	1.2052987911400024
1201	24	0.8289367550921725
1201	25	1.1034529493855771
1202	1	0.6042847610854274
1202	2	0.7461628598060379
1202	3	0.7617591752198329
1202	4	0.6267677170027994
1202	5	0.8328123290909986
1202	6	0.7781945968809012
1202	7	0.8545295664151737
1202	8	0.6363405503305444
1202	9	1.0350251604178453
1202	10	1.0662331123957145
1202	11	1.087119501378993
1202	12	1.0979315408421755
1202	13	1.1307579209221146
1202	14	1.204339746348417
1202	15	1.0419265415957284
1202	16	1.2790380365564715
1202	17	1.1055639880358257
1202	18	1.2873110259395446
1202	19	1.3413690816871622
1202	20	1.1487785578405436
1202	21	1.2802827590536552
1202	22	1.2522449409596916
1202	23	1.3305819018083653
1202	24	1.1500119674540414
1202	25	1.32067292964674
1203	1	0.7340764589837789
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2211	10	0.08828727774256606
2211	11	6.05405718559382E-4
2211	12	-0.04022704853592529
2211	13	0.2729445960356216
2211	14	0.1150223564212709
2211	15	-0.00815528714463909
2211	16	0.07563290510255882
2211	17	-0.08993279160729628
2211	18	-0.1507894914292649
2211	19	-0.08454951191436066
2211	20	-0.007292606767208554
2211	21	0.24745359634644615
2211	22	0.17947543404384447
2211	23	-0.0646139592411501
2211	24	0.2346545606332242
2211	25	0.015398935348358123
2212	1	-0.1979413656652902
2212	2	-0.103779638490483
2212	3	-0.10753342736826355
2212	4	-0.1505604696925147
2212	5	-0.5353949567393315
2212	6	-0.20298605517204749
2212	7	-0.44199437006366793
2212	8	-0.3380299064397789
2212	9	-0.23047128831264985
2212	10	-0.5548637642129179
2212	11	-0.3714320359175147
2212	12	-0.17847237849773734
2212	13	-0.1718297301221412
2212	14	0.013694819739335173
2212	15	-0.3619314284528059
2212	16	-0.12415304762959778
2212	17	-0.2923098623020408
2212	18	-0.44004535369964515
2212	19	-0.13612603103998566
2212	20	-0.36928054766206486
2212	21	-0.4394447885024064
2212	22	-0.3776345211873746
2212	23	-0.652018811718591
2212	24	-0.5348690270799423
2212	25	-0.46523699575725885
2213	1	-0.6040374045538144
2213	2	-0.1592975502864995
2213	3	-0.31955558198092504
2213	4	-0.539131957369456
2213	5	-0.3959563419361274
2213	6	-0.35594248958740593
2213	7	-0.7937193514908003
2213	8	-0.6244422593675054
2213	9	-0.5520069625595667
2213	10	-0.3908772041843491
2213	11	-0.349657590953278
2213	12	-0.6223447466757221
2213	13	-0.49204216674120005
2213	14	-0.7657719873564943
2213	15	-1.0086251671725321
2213	16	-1.4484937969246943
2213	17	-1.1560795362070735
2213	18	-0.8633080962130606
2213	19	-0.6492417257778484
2213	20	-0.6073956344945444
2213	21	-0.37616238251815304
2213	22	-0.517670697908919
2213	23	-0.6803597236057044
2213	24	-0.4482853355663394
2213	25	-0.4305998172802319
2214	1	-1.271945848640268
2214	2	-0.5552042647477826
2214	3	-0.546197298136974
2214	4	-0.6580792890962854
2214	5	-0.8931592369073797
2214	6	-1.4607869234503816
2214	7	-1.0041296998708304
2214	8	-0.7996190972240456
2214	9	-0.617403222504727
2214	10	-0.9859290818141062
2214	11	-0.883916703934446
2214	12	-0.5548176063201993
2214	13	-1.0031801518168777
2214	14	-0.6248396140689678
2214	15	-0.6863595436211511
2214	16	-0.7786949659792413
2214	17	-0.9886646879831673
2214	18	-0.9974044820233132
2214	19	-0.955455656380714
2214	20	-1.0804006505558803
2214	21	-1.1699372152852021
2214	22	-1.7587127303223298
2214	23	-1.7616765824817833
2214	24	-1.4086422821400575
2214	25	-1.9122885604019686
2215	1	-1.471537243086395
2215	2	-0.9827333770692519
2215	3	-1.018476301658147
2215	4	-1.051993506107813
2215	5	-1.516084887840865
2215	6	-1.3450080232345378
2215	7	-1.9212843193775164
2215	8	-1.4764976057588264
2215	9	-1.69928808321837
2215	10	-1.64075739714594
2215	11	-1.3586310756841726
2215	12	-1.4634989906995557
2215	13	-1.6307028899796236
2215	14	-1.8845349292821112
2215	15	-1.6320945720411046
2215	16	-1.783108521397054
2215	17	-2.0833945775124008
2215	18	-1.7058834319136338
2215	19	-1.9680644017264797
2215	20	-1.8816609755940163
2215	21	-1.4252420539310648
2215	22	-1.7158607998314324
2215	23	-2.3367927460295093
2215	24	-2.1357293795240153
2215	25	-2.3720742128300785
2216	1	-1.5427808085720365
2216	2	-0.9494692318700437
2216	3	-0.9349123113988611
2216	4	-1.268914405181505
2216	5	-2.1823913039849536
2216	6	-2.3173499414057943
2216	7	-2.1200764247752986
2216	8	-2.216003386402388
2216	9	-4.399928127120994
2216	10	-2.2624478414203324
2216	11	-2.3494629324196588
2216	12	-2.7493039395472003
2216	13	-2.646799684680115
2216	14	-2.9602923586889247
2216	15	-2.9153788404696
2216	16	-2.2379489540268054
2216	17	-2.3989547169905308
2216	18	-2.9232806701837575
2216	19	-3.050787151564869
2216	20	-2.3817693727224594
2216	21	-2.909735004003636
2216	22	-2.4743353624597546
2216	23	-3.572669190474908
2216	24	-2.975870803790734
2216	25	-3.0042807884216955
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45898.0
3	1.0
4	727.0
5	6501.0
6	7883.0
7	6307.0
8	4840.0
9	4146.0
10	3609.0
11	3170.0
12	2936.0
13	2778.0
14	2661.0
15	2679.0
16	2590.0
17	2830.0
18	3103.0
19	3720.0
20	5005.0
21	6671.0
22	8271.0
23	10007.0
24	11879.0
25	13225.0
26	14732.0
27	16168.0
28	18381.0
29	21672.0
30	27435.0
31	34420.0
32	43679.0
33	55991.0
34	71601.0
35	94651.0
36	129694.0
37	199665.0
38	605037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.7124039531716	19.150970814621754	13.651138900756857	26.48548633144979
2	14.172178958374332	20.177062708258866	38.66710309826271	26.98365523510409
3	17.232056215492722	24.796720762924544	29.39494729797557	28.576275723607157
4	12.002845138673745	16.435292834492824	36.10381945815231	35.45804256868112
5	13.54951959723396	38.098813665830804	34.06446103674912	14.287205700186112
6	32.95196331135139	35.65487229270579	17.400863656144345	13.99230073979848
7	28.94594639837956	31.601319115456082	20.94895960627417	18.503774879890187
8	26.71103609831081	34.769370229454815	20.004204406458985	18.515389265775394
9	27.264452621476444	14.292665166602191	19.53361023399132	38.90927197793005
10	16.225906584523848	28.45561817647496	32.50516628163616	22.813308957365038
11	34.97988592657226	22.3652303549873	23.209405838568166	19.445477879872275
12	23.574024660166632	24.937105066655246	29.933103878342465	21.555766394835658
13	29.126164218843364	21.201948266159548	25.528937935316975	24.142949579680113
14	22.001300727711655	21.470638614484113	27.053998934795004	29.474061723009232
15	24.351130172806656	29.131804308154365	23.199710371678524	23.317355147360455
16	23.38933306210068	27.417940969424766	25.82212937262281	23.370596595851747
17	22.35489662214472	27.651092127036424	26.63788944434445	23.356121806474405
18	22.34909087502568	26.372620459979164	28.410347824865053	22.867940840130107
19	23.712990898147982	26.249840250888074	27.603642881947582	22.433525969016365
20	24.49696358171565	25.64288027492366	27.527159148999946	22.332996994360744
21	24.26421439367733	25.54278553071508	26.968891826953634	23.224108248653952
22	24.28933600882672	26.336168740753923	26.797042326367816	22.57745292405154
23	23.415138739551296	26.01114840926746	27.31012342738312	23.263589423798127
24	23.616133946846002	25.67513045619355	27.541763043779355	23.166972553181097
25	23.372450676217728	26.012486593071017	27.611683147515357	23.00337958319589
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	103.0
1	103.0
2	293.0
3	483.0
4	483.0
5	483.0
6	1243.0
7	2003.0
8	2003.0
9	2003.0
10	2262.0
11	2521.0
12	2521.0
13	2521.0
14	3489.5
15	4458.0
16	4458.0
17	4458.0
18	8989.0
19	13520.0
20	13520.0
21	13520.0
22	21800.0
23	30080.0
24	30080.0
25	30080.0
26	45304.0
27	60528.0
28	60528.0
29	60528.0
30	75233.0
31	89938.0
32	89938.0
33	89938.0
34	104587.0
35	119236.0
36	119236.0
37	119236.0
38	132072.5
39	144909.0
40	144909.0
41	144909.0
42	158338.5
43	171768.0
44	171768.0
45	171768.0
46	187803.5
47	203839.0
48	203839.0
49	203839.0
50	201275.0
51	198711.0
52	198711.0
53	198711.0
54	180906.5
55	163102.0
56	163102.0
57	163102.0
58	142803.0
59	122504.0
60	122504.0
61	122504.0
62	102578.0
63	82652.0
64	82652.0
65	82652.0
66	64276.0
67	45900.0
68	45900.0
69	45900.0
70	34383.0
71	22866.0
72	22866.0
73	22866.0
74	16497.0
75	10128.0
76	10128.0
77	10128.0
78	6920.5
79	3713.0
80	3713.0
81	3713.0
82	2445.0
83	1177.0
84	1177.0
85	1177.0
86	761.5
87	346.0
88	346.0
89	346.0
90	206.0
91	66.0
92	66.0
93	66.0
94	38.5
95	11.0
96	11.0
97	11.0
98	6.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0857106726180161
2	0.04208588062196107
3	0.020942576525713536
4	0.005620371974951876
5	0.01980512029268756
6	0.061088090632512644
7	0.0761426584225623
8	0.05968299763877468
9	0.0270982220220894
10	0.0496466191120749
11	0.0058210995454858715
12	4.6836433124598965E-4
13	0.007092374158867843
14	0.0010036378526699777
15	0.015656750501651655
16	0.010103287716877777
17	0.00628946387673186
18	0.006958555778511846
19	0.0021410940856959526
20	0.002609458416941942
21	2.6763676071199407E-4
22	6.690919017799852E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1494563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.53366380571448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23555406694149	42.533415198571255
2	14.169642082996106	16.021235635348116
3	4.755075213025647	8.064654703922344
4	2.1059621702108386	4.762310292730097
5	1.1128722201954955	3.1457371977625606
6	0.6523372822690956	2.212740996224072
7	0.41400550723342167	1.638367371175399
8	0.29767547761631785	1.346294829981312
9	0.20104943097789887	1.022945484531126
>10	0.9570465741020067	9.301582640737111
>50	0.05890690539266004	2.300009490884481
>100	0.034177051814090496	3.8115288669878096
>500	0.003916011842212315	1.6243731062344053
>1k	0.0015426713317806089	1.4173431881804932
>5k	2.373340510431706E-4	0.797460996729376
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6829	0.4569228597255519	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5058	0.3384266839203165	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4085	0.27332404187712395	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2334	0.15616604987544855	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2196	0.14693258163088474	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1638	0.10959725351156159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3381838035599704E-4
2	0.0	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
3	0.0	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
4	0.0	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
5	6.690919017799852E-5	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
6	6.690919017799852E-5	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
7	6.690919017799852E-5	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
8	6.690919017799852E-5	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
9	6.690919017799852E-5	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
10	6.690919017799852E-5	1.3381838035599704E-4	0.0	0.0	1.3381838035599704E-4
11	6.690919017799852E-5	2.0072757053399555E-4	0.0	0.0	1.3381838035599704E-4
12	6.690919017799852E-5	2.0072757053399555E-4	0.0	0.0	4.014551410679911E-4
13	6.690919017799852E-5	2.0072757053399555E-4	0.0	0.0	4.014551410679911E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGAC	160	0.0	14.26292	3
TAGGACC	230	0.0	13.643708	4
TAAGACC	90	5.341062E-7	12.679001	4
GGTATCA	1400	0.0	12.298224	1
GGTTATA	125	1.380613E-9	12.175922	1
TCGTCTC	55	0.0030829601	12.083242	16
TAGACAG	200	0.0	11.886564	5
TAAAACG	105	2.741872E-7	11.754448	12
GTATACG	65	7.935498E-4	11.707619	1
GTTTATA	125	1.7958882E-8	11.4149275	1
GTAGAGC	95	1.346391E-5	11.009974	3
GTTCTAA	105	3.4113982E-6	10.871359	1
GTATCAA	3020	0.0	10.8353615	1
TAGGACT	220	0.0	10.805966	4
GTCCTAT	125	2.1991036E-7	10.653933	1
GTCCTAG	125	2.1991036E-7	10.653933	1
CCCCGTA	190	9.094947E-12	10.493694	15
CCCGTAC	190	9.094947E-12	10.493342	16
GTCCTAC	765	0.0	10.44503	1
GGCGAGG	300	0.0	10.4416275	19
>>END_MODULE