FastQCFastQC Report
Thu 26 May 2016
SRR1512836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107898
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62840.29811689180406264No Hit
GTCCTACAGTGGACATTTCTAAATT56600.26851394137667No Hit
GTCCTAAAGTGTGTATTTCTCATTT46350.2198873000496229No Hit
CTGTAGGACGTGGAATATGGCAAGA43530.20650904360647432No Hit
CTTTAGGACGTGAAATATGGCGAGG36970.17538799315716416No Hit
TATCAACGCAGAGTACTTTTTTTTT36340.17239923373901395No Hit
GGTATCAACGCAGAGTACTTTTTTT35100.16651659615408337No Hit
GTACTGGTTCACTATCGGTCAGTCA32040.1519997646944966No Hit
GTCCTACAGTGTGCATTTCTCATTT26290.12472140492566527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC402.7635443E-416.62354518
TAAGACG551.9641047E-413.8153334
GGTATCA18900.013.054581
TAGGACC7950.012.6640554
GCGCCAC2350.011.31788913
TCAAGAC1701.2732926E-1111.1741663
CTAAGGC1201.2795681E-711.0810483
TACACCG600.005885860411.0810485
GTCCTAA13700.011.05608751
GGCGTGC2150.011.0444978
GGCGAGG10500.010.94614219
GCGTGCG2200.010.7934859
TAATACC1502.1409505E-910.7644474
GTCCTAC24450.010.7146681
AATGTCC6250.010.6380598
AGGACCT15450.010.4509195
GCCGGTT1553.6743586E-910.41770111
AAATGTC6200.010.4174547
CTTACAC2100.010.4026163
GTATCAA39750.010.3531261
Produced by FastQC (version 0.11.5)