##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512836_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2107898
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.910133222765047	31.0	30.0	34.0	22.0	34.0
2	29.0474771549667	31.0	30.0	34.0	22.0	34.0
3	29.20954809008785	31.0	30.0	34.0	22.0	34.0
4	32.610575084752675	35.0	33.0	37.0	25.0	37.0
5	32.78724824445965	35.0	33.0	37.0	26.0	37.0
6	32.69607210595579	36.0	33.0	37.0	25.0	37.0
7	32.61841132730331	36.0	33.0	37.0	25.0	37.0
8	32.54268327974124	36.0	33.0	37.0	24.0	37.0
9	33.834370069139965	38.0	34.0	39.0	23.0	39.0
10	33.695744765638565	38.0	34.0	39.0	22.0	39.0
11	33.75684781711449	38.0	34.0	39.0	22.0	39.0
12	33.66273984794331	38.0	34.0	39.0	21.0	39.0
13	33.62802991416093	38.0	34.0	39.0	19.0	39.0
14	34.47157167946457	39.0	34.0	41.0	18.0	41.0
15	34.648365338360776	39.0	34.0	41.0	19.0	41.0
16	34.588917490315	39.0	34.0	41.0	19.0	41.0
17	34.35694611409091	39.0	34.0	41.0	17.0	41.0
18	34.36015642123101	39.0	34.0	41.0	17.0	41.0
19	34.48210729361667	39.0	34.0	41.0	18.0	41.0
20	34.44458223310615	39.0	34.0	41.0	18.0	41.0
21	34.34293737173241	39.0	34.0	41.0	18.0	41.0
22	34.355066516501275	39.0	34.0	41.0	18.0	41.0
23	34.01910433996332	39.0	34.0	41.0	10.0	41.0
24	34.08544483651486	39.0	34.0	41.0	8.0	41.0
25	33.794610080753436	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.766853388564197
1101	2	0.4638135087939048
1101	3	0.7604882749931612
1101	4	0.8862900142336869
1101	5	0.8046625483911853
1101	6	0.6795635646926428
1101	7	0.46206030920794205
1101	8	0.8330208681387319
1101	9	0.7713730929032039
1101	10	0.7148511257291261
1101	11	0.898559462607686
1101	12	0.7407996088773245
1101	13	1.2017138594821901
1101	14	1.187919206397389
1101	15	1.0164734779120437
1101	16	0.8983949625186654
1101	17	0.40532871206042387
1101	18	0.7361454232229363
1101	19	0.2268499226506009
1101	20	-0.5960907607678365
1101	21	0.61593409010041
1101	22	0.7888778869795061
1101	23	0.11607499582922287
1101	24	0.6625681671830961
1101	25	0.5079399621651746
1102	1	0.4983796176913664
1102	2	0.6386936566813652
1102	3	0.46960097496083364
1102	4	0.5277112011573593
1102	5	0.5017036906308974
1102	6	0.36267832616520224
1102	7	0.41125422708140746
1102	8	0.4312019395807525
1102	9	0.5938249534842015
1102	10	0.7091393408233699
1102	11	0.4285371719333
1102	12	0.560428374923454
1102	13	0.4326551771357998
1102	14	0.6518908308846818
1102	15	0.7048256454650641
1102	16	0.6299254975177533
1102	17	0.8820613834682547
1102	18	0.788307671774902
1102	19	0.6488355397557939
1102	20	0.8018274142900452
1102	21	0.9459233794730508
1102	22	0.8144797768724956
1102	23	0.7782062356903836
1102	24	0.10549516332348219
1102	25	0.5319383270332168
1103	1	0.615257944995971
1103	2	0.7448332147163192
1103	3	0.8891098822174612
1103	4	1.019899158772823
1103	5	0.8204701469023234
1103	6	0.4553261897015233
1103	7	0.2786813464175495
1103	8	0.613106499891181
1103	9	0.7831187893515832
1103	10	0.8406714683747367
1103	11	0.6436326170779267
1103	12	0.7252280263722142
1103	13	0.851470576883635
1103	14	1.0203340661548879
1103	15	0.9040081561625612
1103	16	0.9356312087018779
1103	17	0.5750227606892153
1103	18	0.8508069619469438
1103	19	0.7916531941408067
1103	20	1.0421006249490077
1103	21	0.8679436245266743
1103	22	0.9213244679450838
1103	23	0.2868813394504599
1103	24	0.42147229751794413
1103	25	0.7569050087147033
1104	1	0.9173680418809553
1104	2	0.8787672844514418
1104	3	0.8774292463703404
1104	4	1.0723610754547295
1104	5	0.8996812976247668
1104	6	0.7626445748357611
1104	7	0.927115200754578
1104	8	0.728316455541119
1104	9	1.0061265930840975
1104	10	1.0550452492464615
1104	11	0.906408495604424
1104	12	1.1320770299057514
1104	13	1.180682274079821
1104	14	1.3872231607510699
1104	15	1.140910470177218
1104	16	1.1359300118019675
1104	17	1.3283933014525218
1104	18	1.2437735213386247
1104	19	0.8288244256993949
1104	20	0.9358908732867874
1104	21	1.0774881513385566
1104	22	0.7896750082065154
1104	23	1.282859467560172
1104	24	1.2063656297775651
1104	25	0.6428506920224137
1105	1	0.6253986599336478
1105	2	0.5912251510527966
1105	3	0.62275949355638
1105	4	0.7636461041825555
1105	5	0.5745553826091125
1105	6	0.7422572137733567
1105	7	0.7499563030979033
1105	8	0.7694234188140854
1105	9	0.4867296867652797
1105	10	0.6464051245456872
1105	11	0.7111084068808324
1105	12	0.8993011014424539
1105	13	0.9051216890606071
1105	14	0.9150166913830304
1105	15	0.8284134971869008
1105	16	0.8119822690667036
1105	17	1.2365564037759285
1105	18	0.8907744717767301
1105	19	0.7005364062001291
1105	20	0.9195770514710944
1105	21	0.7311716228180316
1105	22	0.8767545630016542
1105	23	0.9816430647914984
1105	24	1.0912587132192968
1105	25	1.3577998090630103
1106	1	0.5332595402177667
1106	2	0.5822078131680541
1106	3	0.5330523181891635
1106	4	0.6541665855286709
1106	5	0.4207861741637231
1106	6	0.6098597354385333
1106	7	0.5636171425802416
1106	8	0.6153528662085179
1106	9	0.7490327175907439
1106	10	0.5621727871206801
1106	11	0.32024994597009027
1106	12	0.5041699466945033
1106	13	0.5949914968899535
1106	14	0.7551451739014823
1106	15	0.5426533915620055
1106	16	0.6711184205964855
1106	17	0.8189641543908266
1106	18	0.6422169010344874
1106	19	0.8189930356279831
1106	20	0.8719352221719632
1106	21	0.8831966986748654
1106	22	0.5709864981555555
1106	23	0.9014855181855168
1106	24	0.559008063838796
1106	25	0.798656976959478
1107	1	0.7258329636793697
1107	2	0.8027858066132723
1107	3	0.6414615824712442
1107	4	0.7674972347912572
1107	5	0.7379488641676772
1107	6	0.8528507791182491
1107	7	0.5177696825587645
1107	8	0.7053635318580689
1107	9	0.5788608664295296
1107	10	0.6994158579801493
1107	11	0.7711757737940204
1107	12	0.6825651196051652
1107	13	0.6542052760615604
1107	14	0.916503560916226
1107	15	0.7232336099606016
1107	16	0.7660893196189207
1107	17	1.0415942258718687
1107	18	0.9972799060635822
1107	19	0.840342346245599
1107	20	1.0254520611842253
1107	21	0.8613566622280118
1107	22	0.8393637301722805
1107	23	1.2116118814109242
1107	24	0.9854588747106945
1107	25	1.2935238682404844
1108	1	0.8391376605565455
1108	2	0.8623544790917848
1108	3	0.8625057659920685
1108	4	0.9123606894599519
1108	5	0.8964557647192635
1108	6	0.9378442737598363
1108	7	0.8852588493941198
1108	8	0.9172918981605633
1108	9	1.0038766363590668
1108	10	1.008715286734926
1108	11	0.8628498389584749
1108	12	0.9904758706233849
1108	13	0.8748906359393374
1108	14	0.8917502731694
1108	15	0.912497206747652
1108	16	0.7668173821980275
1108	17	0.9860018434827893
1108	18	1.1594802039073713
1108	19	1.0331196765204211
1108	20	1.154465539749303
1108	21	1.061567403922865
1108	22	1.041182458716989
1108	23	1.3316809751208396
1108	24	1.222805847598174
1108	25	1.3102027840756776
1109	1	0.7905589197597926
1109	2	0.7598224779150726
1109	3	0.8668612788363603
1109	4	0.8717125199863673
1109	5	0.7951541468772803
1109	6	0.8610338064655423
1109	7	0.7196143316656674
1109	8	0.7099941318056722
1109	9	0.9430329575468619
1109	10	0.9557800989265246
1109	11	0.9570827822514616
1109	12	0.9028806110301701
1109	13	1.0108226020592923
1109	14	1.1468971106901833
1109	15	0.9688690264173445
1109	16	0.6522133061031354
1109	17	1.1916224397329387
1109	18	1.0897007896948452
1109	19	1.0233628080124575
1109	20	0.9806439154394795
1109	21	0.9192736731709559
1109	22	1.016017263958382
1109	23	1.1669669730052092
1109	24	0.9695215699405395
1109	25	0.6915915546367017
1110	1	0.42423199515065946
1110	2	0.501010226771534
1110	3	0.4068864377265484
1110	4	0.2942000529033493
1110	5	0.21265127142962115
1110	6	0.29802779407302893
1110	7	0.27933886071534886
1110	8	0.3255792113478009
1110	9	0.510895054550069
1110	10	0.4198717332635553
1110	11	0.422809029419426
1110	12	0.3638370880909392
1110	13	0.34490288782669154
1110	14	0.31760140639447343
1110	15	0.3084233882109686
1110	16	0.47016810181866475
1110	17	0.6273927021903134
1110	18	-0.08975625632661632
1110	19	0.29396822509026777
1110	20	0.4461530995069438
1110	21	0.5625363409735016
1110	22	0.6114236469414465
1110	23	0.7197579958882585
1110	24	0.5547290516204129
1110	25	0.9031300161751616
1111	1	0.43540248711367013
1111	2	0.45337499689481575
1111	3	0.6011385170175458
1111	4	0.45027086990594256
1111	5	0.508914409400063
1111	6	0.3243205197522556
1111	7	0.31048084091756323
1111	8	0.4751880569141491
1111	9	0.6079348892431398
1111	10	0.5855926846907877
1111	11	0.3872823459955157
1111	12	0.09807647291724919
1111	13	0.4688255841488953
1111	14	0.48704608467828336
1111	15	0.5175887951577351
1111	16	0.5525816717779222
1111	17	0.09250573439938137
1111	18	0.38395549393776207
1111	19	0.47830712219366234
1111	20	0.7117917452172478
1111	21	0.5774858204815132
1111	22	0.2455363453484054
1111	23	0.6004030860965983
1111	24	0.6963034062538966
1111	25	0.7850785528084359
1112	1	0.45932082335018976
1112	2	0.43133617383509915
1112	3	0.43712833953877706
1112	4	0.6473622586967949
1112	5	0.5582391269807303
1112	6	0.23783561796392405
1112	7	0.2317377989342475
1112	8	0.3112874075712426
1112	9	0.13765268844628054
1112	10	0.3087655040227446
1112	11	0.2497893793775816
1112	12	0.2905458262019778
1112	13	0.40659097177225334
1112	14	0.33875146712237125
1112	15	0.13402705074423693
1112	16	0.3145816106218362
1112	17	0.6008368693959127
1112	18	0.2426402717900018
1112	19	0.3267905807674083
1112	20	0.12107834907971693
1112	21	-0.08002108074121139
1112	22	-0.13696501468353262
1112	23	0.06136995348997942
1112	24	0.15960819835170525
1112	25	0.12007519354250462
1113	1	0.16493549987655243
1113	2	0.2985083137308173
1113	3	0.33479503189095894
1113	4	0.3709850500746086
1113	5	0.1963728174312962
1113	6	0.06268909981267967
1113	7	0.023787600491502303
1113	8	0.09039100079770179
1113	9	0.1448664211628028
1113	10	0.28167640805546057
1113	11	0.13194264041947434
1113	12	0.024874251830453886
1113	13	0.16741648652326546
1113	14	0.47865736956524074
1113	15	0.18662460920313606
1113	16	0.1386795840346693
1113	17	0.2915981437871906
1113	18	0.13906839087513134
1113	19	-0.13855576183662066
1113	20	0.13732160331051801
1113	21	0.27896986821458825
1113	22	-0.17259374738677025
1113	23	-4.043511445856897
1113	24	-1.5198320522680717
1113	25	-0.5206363483766978
1114	1	-0.4110550105707844
1114	2	-0.6589856305372557
1114	3	-0.4857528055121598
1114	4	-0.4384085321931366
1114	5	-0.3583713830777313
1114	6	-1.1267595310736667
1114	7	-0.8627615728195153
1114	8	-0.7230513804965142
1114	9	-0.8773850826932872
1114	10	-0.602269856200941
1114	11	-0.7192059332685119
1114	12	-1.6958578487311655
1114	13	-0.8216588195263199
1114	14	-2.683820509258698
1114	15	-1.3468636843280706
1114	16	-1.38791688348703
1114	17	-1.2215069975388104
1114	18	-1.0995176581004813
1114	19	-1.347942242295133
1114	20	-0.8403579860897423
1114	21	-0.7527147577120061
1114	22	-0.9823733225834133
1114	23	-5.411501096409495
1114	24	-2.3115415537210637
1114	25	-2.2822787089413126
1115	1	-1.4925481141962713
1115	2	-1.2778594809616983
1115	3	-1.0967529730227206
1115	4	-1.0960161596904463
1115	5	-1.0121292098812233
1115	6	-1.1776862724675645
1115	7	-1.628028865931224
1115	8	-1.8611522657917945
1115	9	-3.1490788773096448
1115	10	-3.757161713814611
1115	11	-2.4555300885661033
1115	12	-2.695115133577186
1115	13	-2.0839094514177425
1115	14	-2.838560842388951
1115	15	-2.3514337412071242
1115	16	-2.4172924300049488
1115	17	-2.3333739439389625
1115	18	-5.056720376871429
1115	19	-2.992434518567517
1115	20	-3.380354957561554
1115	21	-2.8876144063321263
1115	22	-2.395849311886277
1115	23	-2.8902074441182677
1115	24	-2.6484028412456837
1115	25	-2.8607888888366695
1116	1	-1.1901927118231939
1116	2	-1.2035848590077052
1116	3	-1.6220175185341255
1116	4	-1.3271416741172324
1116	5	-1.4446023357533129
1116	6	-3.4724219301968446
1116	7	-1.9966140163916606
1116	8	-2.4543293712945555
1116	9	-3.190319240532176
1116	10	-2.738822804108686
1116	11	-2.2807576631305544
1116	12	-2.0131092779407176
1116	13	-3.2679521663235533
1116	14	-6.479932809191997
1116	15	-4.487758372312193
1116	16	-3.2801946461979306
1116	17	-4.325469138522536
1116	18	-2.94885111762191
1116	19	-2.632975608124056
1116	20	-3.442068467189767
1116	21	-4.089634283480464
1116	22	-3.698687568026738
1116	23	-2.5958886393159624
1116	24	-3.61551305102045
1116	25	-3.621018835764662
1201	1	0.4374764104664912
1201	2	0.48287775805943767
1201	3	0.9492707660450925
1201	4	0.7249697813364762
1201	5	0.19887673127740868
1201	6	0.7465288008062103
1201	7	0.8907645950367566
1201	8	0.8916839915068238
1201	9	1.2743182552688026
1201	10	1.0854644045832273
1201	11	1.3101481088111413
1201	12	1.1028799529680313
1201	13	1.202369501416733
1201	14	1.3289086896591016
1201	15	1.3018495653263642
1201	16	1.2260548242671092
1201	17	1.4098906834089604
1201	18	1.2314239765874149
1201	19	1.5020638957160557
1201	20	1.407935726514225
1201	21	1.5586051697797458
1201	22	1.342678212796244
1201	23	1.6323474206629456
1201	24	1.403172454814758
1201	25	1.5297506769865379
1202	1	0.7030955651185238
1202	2	0.7170049682263979
1202	3	0.685472473367799
1202	4	0.8093767984481914
1202	5	0.7035364598608211
1202	6	0.9393511945988848
1202	7	0.9288568274112308
1202	8	0.98884243074599
1202	9	1.1564691123940634
1202	10	0.9388317098922414
1202	11	0.9703112204798998
1202	12	1.0411523086576935
1202	13	0.9449321019663373
1202	14	1.4879052334467673
1202	15	1.0578829266275633
1202	16	0.8578981631683575
1202	17	1.174047683201124
1202	18	1.157512221616706
1202	19	1.1969334602640913
1202	20	1.0747245280783773
1202	21	1.1223668989635698
1202	22	1.1929457036168785
1202	23	1.290280928987876
1202	24	1.2606741290378807
1202	25	1.5852363556434668
1203	1	0.9515618227872338
1203	2	0.9387841093306903
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2211	12	0.030835249157867395
2211	13	0.030442325958716765
2211	14	0.44874310535657
2211	15	-0.03725367065484164
2211	16	-0.0638822213815402
2211	17	0.2932876127264521
2211	18	-0.08648777073132408
2211	19	0.1624518527376111
2211	20	-0.00814672173749642
2211	21	0.33717289275399764
2211	22	0.28695621742773625
2211	23	0.5701761352181123
2211	24	0.27763826615358056
2211	25	0.6228057255608803
2212	1	0.17175673920462486
2212	2	0.2517173692123542
2212	3	-0.11367694124369265
2212	4	0.21967266077519554
2212	5	-0.10076102692634237
2212	6	-0.11729762306110558
2212	7	0.08987222781997417
2212	8	0.11524376265290925
2212	9	0.15220791273775802
2212	10	-0.1303717969863314
2212	11	-0.13361154997673452
2212	12	0.3111391851253984
2212	13	0.3183140813635177
2212	14	0.39480176247929677
2212	15	-0.0656328263115995
2212	16	-0.08670398510498956
2212	17	0.17542847433578856
2212	18	0.5502664524517442
2212	19	0.30850313249541017
2212	20	0.32650870801742116
2212	21	0.36011171958669763
2212	22	0.5044516383489253
2212	23	0.5768971822382056
2212	24	0.5208860421217381
2212	25	0.6167882039369346
2213	1	-0.3273047724470217
2213	2	-0.12639164284309956
2213	3	-0.42244988655672344
2213	4	-0.22121413090010122
2213	5	-0.27332418056549557
2213	6	-0.036730484884529346
2213	7	-0.2734957010116048
2213	8	-0.20468550849221145
2213	9	-0.5130200826638287
2213	10	-0.2497024401727117
2213	11	-0.38873051058656216
2213	12	-0.3214627812807649
2213	13	-0.2931549633943149
2213	14	-0.4186464776122918
2213	15	-0.3953064040254759
2213	16	-0.2910023782640181
2213	17	-0.3058738043629958
2213	18	-0.38467493761751115
2213	19	-0.22710528391621665
2213	20	-0.0807926773340526
2213	21	-0.4141641409469159
2213	22	-0.46888805483411033
2213	23	-0.15286393024918965
2213	24	0.009279300617897945
2213	25	0.160420072846982
2214	1	-2.31262881512437
2214	2	-1.0364021308095381
2214	3	-1.3873971115827466
2214	4	-3.0963023520463615
2214	5	-1.487214526116393
2214	6	-1.2244677455538664
2214	7	-1.1067259946746617
2214	8	-0.979216211422532
2214	9	-0.7704938430335062
2214	10	-0.7627446798661168
2214	11	-0.7398928611527538
2214	12	-0.6779091349381474
2214	13	-1.1595353138343398
2214	14	-0.9366502942857977
2214	15	-1.3429508501346774
2214	16	-1.208389632624801
2214	17	-3.333997030082962
2214	18	-2.9271045775569533
2214	19	-1.8775168183135946
2214	20	-1.6392317446147544
2214	21	-1.6479782713131286
2214	22	-2.616582279355452
2214	23	-1.5930497959401322
2214	24	-2.9455187468854938
2214	25	-2.7868147117159907
2215	1	-1.8874164135792917
2215	2	-3.413108577876649
2215	3	-2.12926317829303
2215	4	-3.548692085683342
2215	5	-2.3807459907984416
2215	6	-2.336591934008535
2215	7	-2.5567876469557973
2215	8	-1.8779024044570818
2215	9	-4.254908948471492
2215	10	-2.6851945244772004
2215	11	-2.257124044624913
2215	12	-2.0529162123683804
2215	13	-2.1651713074426304
2215	14	-1.9193341816508536
2215	15	-2.0691710777448336
2215	16	-2.18106721321314
2215	17	-2.5704126891332635
2215	18	-2.1388207914777837
2215	19	-2.38925945464063
2215	20	-2.1245303492600627
2215	21	-2.703586820219744
2215	22	-2.5843972721836543
2215	23	-2.6517311291392573
2215	24	-2.5368128785948976
2215	25	-3.4044121128836125
2216	1	-2.803730573951295
2216	2	-2.9162910401007593
2216	3	-4.185518111267967
2216	4	-4.4700489747314265
2216	5	-4.299938314217751
2216	6	-3.3537000458004442
2216	7	-5.251006200330188
2216	8	-3.0774854194913637
2216	9	-3.8501894584261933
2216	10	-2.779566495353233
2216	11	-2.907709345072803
2216	12	-3.358498755104982
2216	13	-2.988349712344384
2216	14	-3.9295218645077163
2216	15	-3.4597133166894203
2216	16	-3.7118267883354186
2216	17	-3.3742403607056985
2216	18	-2.950718730801796
2216	19	-3.5620288782789693
2216	20	-4.190552176386433
2216	21	-3.8952723206809488
2216	22	-3.5760872895857645
2216	23	-3.708419529951989
2216	24	-4.118565150536931
2216	25	-4.150426110645743
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101207.0
3	1.0
4	879.0
5	5570.0
6	6818.0
7	6414.0
8	5511.0
9	5061.0
10	4617.0
11	4251.0
12	4181.0
13	4045.0
14	4044.0
15	4042.0
16	4302.0
17	4544.0
18	5079.0
19	6218.0
20	7964.0
21	10477.0
22	13149.0
23	15953.0
24	18500.0
25	20901.0
26	23486.0
27	25668.0
28	29616.0
29	34794.0
30	42010.0
31	51942.0
32	64920.0
33	82523.0
34	106251.0
35	138205.0
36	187064.0
37	283386.0
38	774305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.90059945908742	18.395073716236993	12.58369056220828	24.12063626246731
2	14.723113126980813	19.981971039796555	42.178515172793354	23.116400660429278
3	19.32991353723061	25.737571451344763	28.808650831606354	26.12386417981827
4	12.169777079809556	16.344365079553352	38.311243746159924	33.174614094477164
5	13.015887626098483	38.994381494706495	34.79824717058298	13.191483708612036
6	31.586818671270105	36.21936026648283	18.767602357172837	13.426218705074227
7	28.571442136498515	31.745234421364987	21.62212462908012	18.061198813056382
8	25.735709638662925	36.489678457965894	20.737490443016636	17.037121460354545
9	28.021289829405983	14.585764681863287	20.027775535688004	37.365169953042724
10	16.4224376369589	28.58616866451569	33.29520445011075	21.69618924841466
11	34.59570025112493	22.279752891925114	24.623395689615467	18.50115116733448
12	24.369433454074393	24.554676325951476	31.041120973424935	20.034769246549192
13	29.453524384795333	22.02563042187023	25.825657865062112	22.69518732827233
14	21.413119757505083	22.617095245918296	27.482424911186808	28.487360085389813
15	23.505638441775982	31.314753293542253	23.147547179307242	22.032061085374522
16	22.056255371276904	27.92408753578873	28.04003589556101	21.979621197373355
17	21.694113133037998	28.568559023713387	27.614743071348148	22.122584771900467
18	21.4827238894743	26.97021170305063	30.196355161178957	21.35070924629611
19	23.78433434871281	26.558670810491254	27.53856344776335	22.11843139303258
20	24.44739057618771	26.575823032028577	28.100446209168915	20.876340182614793
21	23.80544519481309	26.428385584819086	26.80726266531955	22.958906555048276
22	24.026325249511945	28.023882485180295	26.766314735747894	21.183477529559873
23	22.628229053001057	27.646562751963717	27.855351341289808	21.86985685374542
24	23.549454995071308	26.36004322813095	28.365042193010947	21.72545958378679
25	22.838316768310634	27.220323981835136	28.291859049478546	21.649500200375684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	262.0
1	262.0
2	627.5
3	993.0
4	993.0
5	993.0
6	1785.0
7	2577.0
8	2577.0
9	2577.0
10	2963.0
11	3349.0
12	3349.0
13	3349.0
14	4989.5
15	6630.0
16	6630.0
17	6630.0
18	13217.0
19	19804.0
20	19804.0
21	19804.0
22	33690.5
23	47577.0
24	47577.0
25	47577.0
26	72995.5
27	98414.0
28	98414.0
29	98414.0
30	129158.5
31	159903.0
32	159903.0
33	159903.0
34	180326.5
35	200750.0
36	200750.0
37	200750.0
38	213682.0
39	226614.0
40	226614.0
41	226614.0
42	240659.0
43	254704.0
44	254704.0
45	254704.0
46	269651.0
47	284598.0
48	284598.0
49	284598.0
50	274786.5
51	264975.0
52	264975.0
53	264975.0
54	233791.5
55	202608.0
56	202608.0
57	202608.0
58	173825.5
59	145043.0
60	145043.0
61	145043.0
62	120827.5
63	96612.0
64	96612.0
65	96612.0
66	74266.0
67	51920.0
68	51920.0
69	51920.0
70	38394.0
71	24868.0
72	24868.0
73	24868.0
74	17451.0
75	10034.0
76	10034.0
77	10034.0
78	6947.5
79	3861.0
80	3861.0
81	3861.0
82	2588.0
83	1315.0
84	1315.0
85	1315.0
86	846.5
87	378.0
88	378.0
89	378.0
90	230.5
91	83.0
92	83.0
93	83.0
94	51.5
95	20.0
96	20.0
97	20.0
98	13.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3322741422972079
2	0.008444431371916477
3	0.008064906366437086
4	0.009156040757190339
5	0.010436937650683287
6	0.07808726987738496
7	0.0781821511287548
8	0.10000483894381987
9	0.08197740118354874
10	0.08933069816471195
11	0.08420711059074015
12	0.09554542012943701
13	0.08202484180923365
14	0.08501360122738387
15	0.0821197230606035
16	0.08482383872464416
17	0.09682631702292996
18	0.09824953579347767
19	0.09265154196265664
20	0.091797610700328
21	0.07941560739656282
22	0.09900858580443647
23	0.09203481382875263
24	0.08833444502532856
25	0.08795492001984916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2107898.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.53048335599189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.37716018530276	39.814453353652915
2	14.062838532701807	15.05581088025591
3	5.07300508011828	8.146812420184016
4	2.3131869060297667	4.953040526900991
5	1.231300582491968	3.2956057668654704
6	0.7395069076296822	2.3751697326307037
7	0.49189691297999655	1.8432035659197648
8	0.3236325609625124	1.385936593444864
9	0.24051435461316686	1.1587364690847533
>10	1.032219475459287	9.41512688578281
>50	0.06178833572598001	2.302155773737219
>100	0.044306440304683184	4.6330628721281855
>500	0.005613966163320381	2.2095251884753346
>1k	0.0028515383686706602	2.8416153929763355
>5k	1.7822114804191626E-4	0.5697445779609097
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6284	0.29811689180406264	No Hit
GTCCTACAGTGGACATTTCTAAATT	5660	0.26851394137667	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4635	0.2198873000496229	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4353	0.20650904360647432	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3697	0.17538799315716416	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3634	0.17239923373901395	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3510	0.16651659615408337	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3204	0.1519997646944966	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2629	0.12472140492566527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.744062568492403E-5
3	0.0	0.0	0.0	0.0	4.744062568492403E-5
4	0.0	0.0	0.0	0.0	4.744062568492403E-5
5	4.744062568492403E-5	0.0	0.0	0.0	4.744062568492403E-5
6	4.744062568492403E-5	0.0	0.0	0.0	4.744062568492403E-5
7	4.744062568492403E-5	0.0	0.0	0.0	4.744062568492403E-5
8	4.744062568492403E-5	0.0	0.0	0.0	4.744062568492403E-5
9	4.744062568492403E-5	0.0	0.0	0.0	9.488125136984806E-5
10	9.488125136984806E-5	0.0	0.0	4.744062568492403E-5	9.488125136984806E-5
11	9.488125136984806E-5	0.0	0.0	4.744062568492403E-5	9.488125136984806E-5
12	9.488125136984806E-5	0.0	0.0	4.744062568492403E-5	9.488125136984806E-5
13	9.488125136984806E-5	0.0	0.0	4.744062568492403E-5	9.488125136984806E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCC	40	2.7635443E-4	16.623545	18
TAAGACG	55	1.9641047E-4	13.815333	4
GGTATCA	1890	0.0	13.05458	1
TAGGACC	795	0.0	12.664055	4
GCGCCAC	235	0.0	11.317889	13
TCAAGAC	170	1.2732926E-11	11.174166	3
CTAAGGC	120	1.2795681E-7	11.081048	3
TACACCG	60	0.0058858604	11.081048	5
GTCCTAA	1370	0.0	11.0560875	1
GGCGTGC	215	0.0	11.044497	8
GGCGAGG	1050	0.0	10.946142	19
GCGTGCG	220	0.0	10.793485	9
TAATACC	150	2.1409505E-9	10.764447	4
GTCCTAC	2445	0.0	10.714668	1
AATGTCC	625	0.0	10.638059	8
AGGACCT	1545	0.0	10.450919	5
GCCGGTT	155	3.6743586E-9	10.417701	11
AAATGTC	620	0.0	10.417454	7
CTTACAC	210	0.0	10.402616	3
GTATCAA	3975	0.0	10.353126	1
>>END_MODULE