FastQCFastQC Report
Thu 26 May 2016
SRR1512836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107898
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82720.3924288556656916No Hit
TATCAACGCAGAGTACTTTTTTTTT61000.2893878166780366No Hit
GTCCTACAGTGGACATTTCTAAATT50410.23914819407770205No Hit
GGTATCAACGCAGAGTACTTTTTTT47380.22477368449517007No Hit
CTGTAGGACGTGGAATATGGCAAGA44330.2103042936612682No Hit
GTCCTAAAGTGTGTATTTCTCATTT42520.20171754041229698No Hit
CTTTAGGACGTGAAATATGGCGAGG39790.1887662496003127No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29860.14165770829518315No Hit
GTACTGGTTCACTATCGGTCAGTCA28370.1345890550681295No Hit
GTCCTACAGTGTGCATTTCTCATTT24090.11428446727498198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA250.00599009919.0233652
TACACCG551.9426853E-413.8332025
GGTATCA17750.012.80728151
TAGGACC7800.012.5585094
GCGTGCG2200.012.0876769
CGGCAAA550.00308726712.081367517
CGTGCGC2050.012.0441310
AATGTCC5450.011.3312818
TCCAACG2100.011.30033218
GTGCGCC2450.011.23629111
GGTACGA600.005912509311.07432518
TATACTG2450.010.8689465
GCGCCAC2450.010.84857713
TGGTACG700.001501640110.84857617
GGCGAGG11100.010.77476319
GTATCAA39100.010.728011
AGGACCT15850.010.5603635
CCAACGT1355.760012E-810.54672619
TCGAACT2250.010.54672519
GGCGTGC2550.010.4322998
Produced by FastQC (version 0.11.5)