##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512836_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2107898 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.455787708892935 31.0 31.0 34.0 27.0 34.0 2 30.457438168260513 31.0 30.0 34.0 27.0 34.0 3 30.629166117146085 31.0 31.0 34.0 27.0 34.0 4 34.2417109366772 37.0 35.0 37.0 30.0 37.0 5 33.87658653312447 37.0 35.0 37.0 30.0 37.0 6 33.77512194612832 37.0 35.0 37.0 29.0 37.0 7 33.624986123616985 37.0 35.0 37.0 28.0 37.0 8 33.573291497026894 37.0 35.0 37.0 28.0 37.0 9 34.917791088563106 39.0 35.0 39.0 27.0 39.0 10 34.8381714864761 39.0 35.0 39.0 27.0 39.0 11 34.833504752127475 39.0 35.0 39.0 27.0 39.0 12 34.70866284801257 39.0 35.0 39.0 27.0 39.0 13 34.70885688017162 39.0 35.0 39.0 27.0 39.0 14 35.79941202088526 40.0 36.0 41.0 27.0 41.0 15 35.80359913050821 40.0 36.0 41.0 27.0 41.0 16 35.80356402444521 40.0 36.0 41.0 27.0 41.0 17 35.80624394539015 40.0 36.0 41.0 27.0 41.0 18 35.7267424704611 40.0 36.0 41.0 27.0 41.0 19 35.7462196937423 40.0 36.0 41.0 27.0 41.0 20 35.7199238293314 40.0 36.0 41.0 27.0 41.0 21 35.691977979959184 40.0 36.0 41.0 27.0 41.0 22 35.65789378802959 40.0 36.0 41.0 27.0 41.0 23 35.50393614871308 40.0 36.0 41.0 27.0 41.0 24 35.46439296398592 40.0 36.0 41.0 27.0 41.0 25 35.40329228454128 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7524415289265995 1101 2 0.5944496377377781 1101 3 0.5974840877612451 1101 4 0.5992345661303133 1101 5 0.6917542887802597 1101 6 0.7160043596652628 1101 7 0.7544806559822987 1101 8 0.532025187378018 1101 9 0.8831615964175157 1101 10 0.64136940400779 1101 11 0.8463128480147972 1101 12 0.9305806894468986 1101 13 1.0154416571034375 1101 14 1.1930646829753258 1101 15 0.8629941848511535 1101 16 1.0692097185459843 1101 17 1.2203675219132109 1101 18 0.9869758138014291 1101 19 1.0151686145945362 1101 20 0.9969626970095149 1101 21 1.033150096949754 1101 22 1.0177240442457034 1101 23 1.1193112594573122 1101 24 1.1197858435106198 1101 25 1.179298074042677 1102 1 0.49877702579261296 1102 2 0.6329748234697306 1102 3 0.32619684185464237 1102 4 0.41435508427878887 1102 5 0.52759383806724 1102 6 0.5195472127561516 1102 7 0.4780569629495588 1102 8 0.5401487186290765 1102 9 0.5239390096823655 1102 10 0.4867444440775941 1102 11 0.6956760416231944 1102 12 0.6384469769158869 1102 13 0.7424493775317842 1102 14 0.5331078168182231 1102 15 0.6598451632964171 1102 16 0.6435437719371464 1102 17 0.7037841891372025 1102 18 -0.029850067823552706 1102 19 0.44508015091405895 1102 20 0.608933277460288 1102 21 0.5462570336452828 1102 22 0.38478725728549534 1102 23 0.5878974044257603 1102 24 0.6652051424468723 1102 25 0.6209770086270439 1103 1 0.824558057883813 1103 2 0.9215283188325323 1103 3 0.6675181654801214 1103 4 0.5635445121659473 1103 5 0.6715781589847793 1103 6 0.7338271717663218 1103 7 0.8964099349310501 1103 8 0.9715532247019354 1103 9 0.9764215832382064 1103 10 0.9531140695397156 1103 11 1.0514076553798475 1103 12 1.260242500497064 1103 13 1.2071708607131768 1103 14 1.1934531468924732 1103 15 1.2736781833217492 1103 16 1.2765481832887815 1103 17 1.1123984556105952 1103 18 1.0503859339889559 1103 19 1.1546348243006364 1103 20 1.0123704276604215 1103 21 1.1586441955916698 1103 22 1.0970906528252442 1103 23 1.2409925181533907 1103 24 1.207951004719952 1103 25 1.1068484587159233 1104 1 0.6490137285019664 1104 2 0.7915627851502087 1104 3 0.6933717071939469 1104 4 0.5555163319576124 1104 5 0.669432834216309 1104 6 0.5393446854629218 1104 7 0.5738767589471792 1104 8 0.6027324109946619 1104 9 0.660150384319401 1104 10 0.6945455142335604 1104 11 0.8935393471585087 1104 12 0.8456881297257368 1104 13 0.7181182162305433 1104 14 0.9542309862541956 1104 15 0.9054300740990442 1104 16 0.9724339704509859 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0.7011457699698624 1107 17 0.5778564848280752 1107 18 0.6705167908797094 1107 19 0.6601574563679762 1107 20 0.6787372999519761 1107 21 0.5688907820551705 1107 22 0.6021712472884246 1107 23 0.8220346949047936 1107 24 0.8620475495166744 1107 25 0.9274538610346426 1108 1 0.4648650139371604 1108 2 0.4041498186462711 1108 3 0.419217806464804 1108 4 0.25350599088426407 1108 5 0.3699179664575212 1108 6 0.4648963697793178 1108 7 0.47648882488772415 1108 8 0.3445337580993595 1108 9 0.5305030221453677 1108 10 0.4007144195703063 1108 11 0.5040060566888158 1108 12 0.43025817637730057 1108 13 0.6161321620852149 1108 14 0.6983411286683392 1108 15 0.7299146621900974 1108 16 0.609163781707224 1108 17 0.6570889785264029 1108 18 0.743890598265665 1108 19 0.683983111082533 1108 20 0.7615393833465518 1108 21 0.7154763718253392 1108 22 0.719244012389133 1108 23 0.8408232955345767 1108 24 0.7597983501923267 1108 25 0.7469001370214272 1109 1 0.3690268314259981 1109 2 0.25588446552081834 1109 3 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-1.529560154439892 2215 6 -1.2670665195365984 2215 7 -1.6957094464045923 2215 8 -1.465998773137379 2215 9 -1.6289372161116802 2215 10 -1.506514081285566 2215 11 -1.4220416637221334 2215 12 -1.6241966366874436 2215 13 -1.5747559506146516 2215 14 -1.8686228441895665 2215 15 -1.750709792220455 2215 16 -1.6395809807239488 2215 17 -1.8141781115182667 2215 18 -1.6212052799262437 2215 19 -1.7540071108045865 2215 20 -1.787146601873431 2215 21 -1.4912885958786362 2215 22 -1.511182601270832 2215 23 -2.088990047736914 2215 24 -2.0137835125726795 2215 25 -2.25757760472613 2216 1 -1.3987567988254987 2216 2 -0.8628505911962421 2216 3 -0.8792462581389664 2216 4 -1.292679870073485 2216 5 -2.0226856873596724 2216 6 -2.0330081511507494 2216 7 -2.1428731244940984 2216 8 -1.940598905000357 2216 9 -4.029690911286316 2216 10 -2.0635685920453994 2216 11 -2.4085688969135646 2216 12 -2.750009686050479 2216 13 -2.5541761338915094 2216 14 -2.723889755774806 2216 15 -2.6222368986205637 2216 16 -2.099627845966964 2216 17 -2.253407822529148 2216 18 -2.7012129824991575 2216 19 -2.8625420687574135 2216 20 -2.1463349313660416 2216 21 -2.8392004220223797 2216 22 -2.2134947995470995 2216 23 -3.376213603029022 2216 24 -2.6113926244311543 2216 25 -2.7808529257459114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48716.0 3 3.0 4 778.0 5 6695.0 6 8881.0 7 6909.0 8 5487.0 9 4688.0 10 3986.0 11 3739.0 12 3300.0 13 3162.0 14 3051.0 15 3082.0 16 3109.0 17 3323.0 18 3837.0 19 4534.0 20 6074.0 21 8041.0 22 10481.0 23 12691.0 24 15260.0 25 17308.0 26 19652.0 27 21614.0 28 24522.0 29 29409.0 30 37354.0 31 47531.0 32 60449.0 33 78151.0 34 101953.0 35 133738.0 36 182981.0 37 284217.0 38 899192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.581810308643966 19.453296969397694 13.728303664185637 26.236589057772697 2 13.360699931844543 20.754048035357304 40.79246379122788 25.09278824157027 3 17.63567086913817 25.650418270074827 29.635632909290198 27.078277951496805 4 11.608874533633427 16.948605739487537 37.463468742527695 33.97905098435134 5 12.640241648608342 39.141020050552235 34.864862684772596 13.353875616066823 6 31.653404864628037 36.8196928147979 18.18190448749381 13.34499783308025 7 28.10000854611579 31.930330164940035 21.69331788702035 18.276343401923825 8 25.708840540842022 36.870055987639525 20.31342635335107 17.107677118167384 9 27.408878449237196 14.449379552518565 19.92122808266306 38.22051391558118 10 15.835299556537757 29.172819272167533 33.315201836312454 21.676679334982254 11 34.50651469584248 22.21415683757217 24.57356650834791 18.70576195823744 12 23.97315610721807 24.862528470400036 31.104739286803728 20.05957613557817 13 29.2983325940036 21.912831838463966 25.928602097482408 22.86023347005003 14 21.180883197816936 22.53462392954381 27.603501745606806 28.680991127032453 15 23.347044206772434 31.31772897071655 23.22866159321646 22.106565229294546 16 22.03992222794727 27.885968483150812 27.97772735942246 22.09638192947946 17 21.425216485337067 28.689881789422344 27.684978014616433 22.19992371062415 18 21.31802879232407 26.88562964706061 30.463130947004057 21.33321061361126 19 23.60514858568554 26.528139456439074 27.822070070750115 22.044641887125263 20 24.2618136618853 26.5559685649019 28.20268189872547 20.97953587448733 21 23.424859906370205 26.269524393275507 27.18727287563405 23.11834282472024 22 23.6956200996357 27.84917919861624 27.2333943267142 21.22180637503386 23 22.304779453275252 27.459108552690882 28.217019988633226 22.01909200540064 24 23.425279591327474 26.348476064781124 28.57690457507906 21.649339768812343 25 22.571063685244734 27.117488607133744 28.515563846068453 21.795883861553072 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 93.0 1 93.0 2 341.0 3 589.0 4 589.0 5 589.0 6 1485.5 7 2382.0 8 2382.0 9 2382.0 10 2788.0 11 3194.0 12 3194.0 13 3194.0 14 4791.0 15 6388.0 16 6388.0 17 6388.0 18 13355.0 19 20322.0 20 20322.0 21 20322.0 22 34795.0 23 49268.0 24 49268.0 25 49268.0 26 75594.5 27 101921.0 28 101921.0 29 101921.0 30 132240.0 31 162559.0 32 162559.0 33 162559.0 34 181928.0 35 201297.0 36 201297.0 37 201297.0 38 215326.0 39 229355.0 40 229355.0 41 229355.0 42 242910.5 43 256466.0 44 256466.0 45 256466.0 46 271087.5 47 285709.0 48 285709.0 49 285709.0 50 274077.0 51 262445.0 52 262445.0 53 262445.0 54 230971.0 55 199497.0 56 199497.0 57 199497.0 58 171483.0 59 143469.0 60 143469.0 61 143469.0 62 118934.0 63 94399.0 64 94399.0 65 94399.0 66 72330.0 67 50261.0 68 50261.0 69 50261.0 70 36984.0 71 23707.0 72 23707.0 73 23707.0 74 16600.0 75 9493.0 76 9493.0 77 9493.0 78 6495.5 79 3498.0 80 3498.0 81 3498.0 82 2339.0 83 1180.0 84 1180.0 85 1180.0 86 759.5 87 339.0 88 339.0 89 339.0 90 198.0 91 57.0 92 57.0 93 57.0 94 33.0 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08453919497053462 2 0.045068594400677835 3 0.019355775279449008 4 0.005408231328081339 5 0.017790234631846515 6 0.05792500396129224 7 0.07960536989930253 8 0.05612226018526513 9 0.025997462875338374 10 0.050619147605813944 11 0.005930078210615503 12 3.3208437979446824E-4 13 0.006309603216094896 14 0.001660421898972341 15 0.015418203347600311 16 0.009488125136984805 17 0.00635704384177982 18 0.005408231328081339 19 0.0027041156640406697 20 0.0029887594181502142 21 0.0010436937650683288 22 9.013718880135567E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2107898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.37931414320478 #Duplication Level Percentage of deduplicated Percentage of total 1 72.35396733964244 37.17497217450668 2 14.97965990440152 15.392893039732295 3 5.446593030138386 8.395266429170093 4 2.6202707278583977 5.385108514675218 5 1.4145260414973353 3.6338688924917752 6 0.8021694991355901 2.4728951215310833 7 0.5388041833154046 1.9378372577364547 8 0.360030463607427 1.4798494632647718 9 0.2593023690804667 1.1990500091156275 >10 1.101177434523289 9.726523314969286 >50 0.06538834712234409 2.303817397445776 >100 0.04924457235882901 4.966919854901773 >500 0.005448949497731634 2.064041203589983 >1k 0.003140072591913135 2.9457832065581373 >5k 2.7706522869821776E-4 0.9211741203111005 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8272 0.3924288556656916 No Hit TATCAACGCAGAGTACTTTTTTTTT 6100 0.2893878166780366 No Hit GTCCTACAGTGGACATTTCTAAATT 5041 0.23914819407770205 No Hit GGTATCAACGCAGAGTACTTTTTTT 4738 0.22477368449517007 No Hit CTGTAGGACGTGGAATATGGCAAGA 4433 0.2103042936612682 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4252 0.20171754041229698 No Hit CTTTAGGACGTGAAATATGGCGAGG 3979 0.1887662496003127 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2986 0.14165770829518315 No Hit GTACTGGTTCACTATCGGTCAGTCA 2837 0.1345890550681295 No Hit GTCCTACAGTGTGCATTTCTCATTT 2409 0.11428446727498198 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.744062568492403E-5 0.0 6 0.0 0.0 0.0 4.744062568492403E-5 4.744062568492403E-5 7 0.0 0.0 0.0 4.744062568492403E-5 4.744062568492403E-5 8 0.0 0.0 0.0 4.744062568492403E-5 4.744062568492403E-5 9 0.0 0.0 0.0 4.744062568492403E-5 4.744062568492403E-5 10 0.0 0.0 0.0 9.488125136984806E-5 4.744062568492403E-5 11 0.0 0.0 0.0 9.488125136984806E-5 4.744062568492403E-5 12 0.0 0.0 0.0 9.488125136984806E-5 4.744062568492403E-5 13 0.0 0.0 0.0 9.488125136984806E-5 4.744062568492403E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTACA 25 0.005990099 19.023365 2 TACACCG 55 1.9426853E-4 13.833202 5 GGTATCA 1775 0.0 12.8072815 1 TAGGACC 780 0.0 12.558509 4 GCGTGCG 220 0.0 12.087676 9 CGGCAAA 55 0.003087267 12.0813675 17 CGTGCGC 205 0.0 12.04413 10 AATGTCC 545 0.0 11.331281 8 TCCAACG 210 0.0 11.300332 18 GTGCGCC 245 0.0 11.236291 11 GGTACGA 60 0.0059125093 11.074325 18 TATACTG 245 0.0 10.868946 5 GCGCCAC 245 0.0 10.848577 13 TGGTACG 70 0.0015016401 10.848576 17 GGCGAGG 1110 0.0 10.774763 19 GTATCAA 3910 0.0 10.72801 1 AGGACCT 1585 0.0 10.560363 5 CCAACGT 135 5.760012E-8 10.546726 19 TCGAACT 225 0.0 10.546725 19 GGCGTGC 255 0.0 10.432299 8 >>END_MODULE