FastQCFastQC Report
Thu 26 May 2016
SRR1512835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064377
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74940.3630150888137196No Hit
GTCCTACAGTGGACATTTCTAAATT55010.2664726452581093No Hit
GTCCTAAAGTGTGTATTTCTCATTT49090.23779571270170127No Hit
CTGTAGGACGTGGAATATGGCAAGA44120.21372065276836547No Hit
TATCAACGCAGAGTACTTTTTTTTT43090.20873125402966608No Hit
GGTATCAACGCAGAGTACTTTTTTT42880.20771399797614487No Hit
CTTTAGGACGTGAAATATGGCGAGG40650.19691170750303846No Hit
GTCCTACAGTGTGCATTTCTCATTT28550.13829838251443413No Hit
GTACTGGTTCACTATCGGTCAGTCA27760.13447156212261616No Hit
CTGTAGGACCTGGAATATGGCGAGA21220.10279130217009781No Hit
CTGAAGGACCTGGAATATGGCGAGA20820.10085367159196212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.013.005021
TAGGACC8700.012.9920974
ACTAGAC1001.446424E-712.3479513
CCAACGA853.9534734E-612.2917819
GTATAGG1950.012.2094371
CGCGCCT2100.011.76080912
GTCCTAT2200.011.687761
TCCTATA2700.011.6091852
GCGCCAC2050.011.58372413
GTTCCGA752.0276527E-411.4280331
GTCCTAC28700.011.282031
ACTGTTC3850.011.1028628
TGTAGGA25600.011.0938632
GTGCGCC2000.010.923717511
ATTATAC2350.010.9130833
CGTGCGC1752.0008883E-1110.85586810
GTATCAA40800.010.8304891
GCGTGCG1855.456968E-1210.7825179
ACCGTGC803.775049E-410.6865048
GTAGGAC25350.010.6787023
Produced by FastQC (version 0.11.5)