##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512835_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2064377
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.882864418659963	31.0	30.0	34.0	22.0	34.0
2	29.022639275674937	31.0	30.0	34.0	22.0	34.0
3	29.18290845131485	31.0	30.0	34.0	22.0	34.0
4	32.580343609718575	35.0	33.0	37.0	25.0	37.0
5	32.796845731181854	35.0	33.0	37.0	26.0	37.0
6	32.679438397153234	37.0	33.0	37.0	25.0	37.0
7	32.630321884035716	37.0	33.0	37.0	25.0	37.0
8	32.50623408418133	37.0	33.0	37.0	23.0	37.0
9	33.81415410072869	39.0	34.0	39.0	22.0	39.0
10	33.690554099372356	38.0	34.0	39.0	20.0	39.0
11	33.745212235943335	38.0	34.0	39.0	22.0	39.0
12	33.64523146692682	38.0	34.0	39.0	19.0	39.0
13	33.61108654087892	38.0	34.0	39.0	19.0	39.0
14	34.468408628850256	39.0	34.0	41.0	17.0	41.0
15	34.649418686606175	39.0	34.0	41.0	18.0	41.0
16	34.58835910301268	39.0	34.0	41.0	18.0	41.0
17	34.356376766453025	39.0	34.0	41.0	17.0	41.0
18	34.361867042696176	39.0	34.0	41.0	17.0	41.0
19	34.47821546161384	39.0	34.0	41.0	18.0	41.0
20	34.45000404480383	39.0	34.0	41.0	18.0	41.0
21	34.342358978035506	39.0	34.0	41.0	17.0	41.0
22	34.351637322058906	39.0	34.0	41.0	16.0	41.0
23	34.02241499493552	39.0	34.0	41.0	10.0	41.0
24	34.09389224933236	39.0	34.0	41.0	2.0	41.0
25	33.80809609872615	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.47956223474080417
1101	2	0.24980123367947726
1101	3	0.49471296811837107
1101	4	0.7195290884425845
1101	5	0.6733446499415479
1101	6	0.6034129803408348
1101	7	0.33145159641735233
1101	8	0.5415848643444434
1101	9	0.6631599681134759
1101	10	0.6211609400529383
1101	11	0.6600331431165216
1101	12	0.5933904121451476
1101	13	0.9491330195091408
1101	14	0.9325633625685015
1101	15	0.77263782916976
1101	16	0.6338452363689697
1101	17	-0.020786538433412716
1101	18	0.47617738973274726
1101	19	0.13265193141918274
1101	20	-0.5117151774319453
1101	21	0.5710559753023219
1101	22	0.5083040302635027
1101	23	0.2056146585941363
1101	24	0.5856334466998021
1101	25	0.2446635103587127
1102	1	1.0777943838138135
1102	2	0.9834699411646817
1102	3	1.0146346359832847
1102	4	1.1355922592044436
1102	5	1.0813176071982227
1102	6	1.0140406215037743
1102	7	0.9440302010049635
1102	8	1.0221021703663737
1102	9	1.4046055686964465
1102	10	1.371760742260534
1102	11	1.1578488464669547
1102	12	1.2588244005573799
1102	13	1.2277509044317014
1102	14	1.4561027526977526
1102	15	1.445604215034109
1102	16	1.3165687775686763
1102	17	1.632413619833045
1102	18	1.529233656466218
1102	19	1.3334991571204853
1102	20	1.519374502554264
1102	21	1.6588302170709994
1102	22	1.5020802182378574
1102	23	1.3385785069901601
1102	24	0.7358462849882415
1102	25	1.1312101095005218
1103	1	0.9366078482218185
1103	2	1.080732210821175
1103	3	1.2204446521497836
1103	4	1.3326913645082357
1103	5	1.117532971517555
1103	6	0.8511899515592916
1103	7	0.8101341260738479
1103	8	1.0532213736595324
1103	9	1.0764425096938055
1103	10	1.3394230097074384
1103	11	1.1057489031961865
1103	12	1.1986383472279556
1103	13	1.3068376259744383
1103	14	1.4068180628131017
1103	15	1.3243722427912843
1103	16	1.3740396995227258
1103	17	0.9582173505073968
1103	18	1.3754625239402856
1103	19	1.3246641541498718
1103	20	1.5738149334596727
1103	21	1.2625214542051992
1103	22	1.2634019708248303
1103	23	0.7043976022008636
1103	24	0.9140886179356542
1103	25	1.1797359435933217
1104	1	1.2721419333926676
1104	2	1.1968186801505318
1104	3	1.2167401291017157
1104	4	1.4174396752784304
1104	5	1.17928775065527
1104	6	1.0753975164233083
1104	7	1.2419377185426441
1104	8	0.9108055225522165
1104	9	1.4194847103815178
1104	10	1.4656343738356483
1104	11	1.218155664924609
1104	12	1.4269336318120835
1104	13	1.4331342090412633
1104	14	1.6681991674852341
1104	15	1.3620297800024375
1104	16	1.5007821911667492
1104	17	1.7932334853572343
1104	18	1.5314898401689092
1104	19	1.1087266736872294
1104	20	1.2941801437417269
1104	21	1.5165417009169104
1104	22	1.1590231024284563
1104	23	1.510267513471213
1104	24	1.3435485934087623
1104	25	0.8576815515958245
1105	1	0.6675786579584226
1105	2	0.7369626007460646
1105	3	0.905052784188566
1105	4	0.9217647373131683
1105	5	0.7171934578513017
1105	6	0.8771220821801364
1105	7	0.8007987607262734
1105	8	0.8659841826018422
1105	9	0.5706658518207277
1105	10	0.6897956641582326
1105	11	0.8024236549990533
1105	12	0.9242562124572444
1105	13	0.9210399162372909
1105	14	1.0759187704629056
1105	15	0.9858734524590105
1105	16	0.8197400075439205
1105	17	1.2135089196004074
1105	18	1.0545618004666792
1105	19	0.7533246885755602
1105	20	0.9846714588782035
1105	21	0.9101893435968123
1105	22	0.7992861297237397
1105	23	1.1761409626027302
1105	24	1.1848314718206723
1105	25	1.2745401123898716
1106	1	0.7415011096494801
1106	2	0.6905655002595559
1106	3	0.6177455803433887
1106	4	0.7518769859992034
1106	5	0.6006154308490892
1106	6	0.7245313746910256
1106	7	0.7731745447086098
1106	8	0.8397116400567555
1106	9	0.8801461277043572
1106	10	0.5319844646896783
1106	11	0.22766505317415664
1106	12	0.5677040739683505
1106	13	0.6683807263035249
1106	14	0.8134154000715483
1106	15	0.6265288714621562
1106	16	0.6726537841407065
1106	17	0.7977834550725547
1106	18	0.8125776657291794
1106	19	0.8343329728998228
1106	20	0.8337779632450619
1106	21	0.7448275882579125
1106	22	0.594015228412708
1106	23	0.9183741155305754
1106	24	0.7292445352621826
1106	25	0.7993823389973969
1107	1	0.9684794501805136
1107	2	1.011683126961998
1107	3	0.999694790334523
1107	4	0.9746808182414242
1107	5	0.9394302042281879
1107	6	1.0200934827086812
1107	7	0.7813567895378739
1107	8	0.9616586694053595
1107	9	0.9441758602912103
1107	10	1.1091518100418298
1107	11	0.9974017168858964
1107	12	1.1043001857879062
1107	13	1.010259018007126
1107	14	1.218873737947888
1107	15	1.023151625044811
1107	16	1.1635080745548834
1107	17	1.4879991964673138
1107	18	1.384061877403198
1107	19	1.315816378684218
1107	20	1.3375232641974293
1107	21	1.1004503750242591
1107	22	1.16667632337969
1107	23	1.546646525187974
1107	24	1.339851654585992
1107	25	1.5955069579775198
1108	1	1.0484502829619267
1108	2	1.0037663104878476
1108	3	0.9785621520589238
1108	4	1.1425108238430184
1108	5	0.8505793165809479
1108	6	0.8439297105032608
1108	7	0.8062820297090525
1108	8	0.8363777861140989
1108	9	0.9145667976564695
1108	10	1.0720310311911092
1108	11	0.7101821431762971
1108	12	0.8457860531167469
1108	13	0.9351034585893956
1108	14	0.975302568813845
1108	15	0.9930085833169073
1108	16	0.809171196119685
1108	17	1.054106047034189
1108	18	1.1520930184980571
1108	19	0.97941083603083
1108	20	1.1288214901889972
1108	21	1.0157877280054777
1108	22	1.054135705418993
1108	23	1.340201646472785
1108	24	1.2819890995001657
1108	25	1.408611060292806
1109	1	0.6666636222301463
1109	2	0.6823386338810131
1109	3	0.7234492094950085
1109	4	0.7907213409811504
1109	5	0.6768169582897414
1109	6	0.7809614261977487
1109	7	0.6360198469405631
1109	8	0.561549305085471
1109	9	0.7257704246672319
1109	10	0.6206233848246612
1109	11	0.5365940686261723
1109	12	0.6501077208809107
1109	13	0.6848912940615008
1109	14	0.8411129905043211
1109	15	0.6667562114657102
1109	16	0.3775664595299659
1109	17	0.8968587022456092
1109	18	0.761729284934674
1109	19	0.7704409594015118
1109	20	0.6625341055693781
1109	21	0.647125853606326
1109	22	0.7325268618269476
1109	23	1.0300454197754405
1109	24	0.8937487090825584
1109	25	0.5190650102424428
1110	1	0.7040405976027095
1110	2	0.6528739542177817
1110	3	0.6281081622661588
1110	4	0.6381563956837724
1110	5	0.4747193489970485
1110	6	0.6037208697167245
1110	7	0.4800178659401624
1110	8	0.5259754903090084
1110	9	0.7366344109803222
1110	10	0.4627020845680292
1110	11	0.48688896755012934
1110	12	0.5791086663583727
1110	13	0.6395745591103505
1110	14	0.6457219680379751
1110	15	0.6150412954331088
1110	16	0.6262220806186178
1110	17	0.7888216935562156
1110	18	0.13509923676034674
1110	19	0.6121518233878263
1110	20	0.6159562837693642
1110	21	0.8250539072734284
1110	22	0.7372506473386338
1110	23	0.9385051919603882
1110	24	0.785733761534047
1110	25	0.8538851444101923
1111	1	0.34445975927935635
1111	2	0.40437096020580654
1111	3	0.646970437715261
1111	4	0.54653962921671
1111	5	0.5220247382186045
1111	6	0.4974334445670223
1111	7	0.4006765485748929
1111	8	0.5493287837221814
1111	9	0.6288499798425988
1111	10	0.6980314258853966
1111	11	0.4856913627597095
1111	12	0.20545597174759678
1111	13	0.5455625830624484
1111	14	0.6789555009721084
1111	15	0.6018676427384975
1111	16	0.7780184878042462
1111	17	0.25229474744843117
1111	18	0.5764166020298092
1111	19	0.5588584102141709
1111	20	0.8629622717933145
1111	21	0.6657724701476795
1111	22	0.44443627608114866
1111	23	0.7783255512418279
1111	24	0.8102846597376399
1111	25	0.9484959228533114
1112	1	0.4325944166172597
1112	2	0.347394361034695
1112	3	0.2962687924094318
1112	4	0.41476406172807856
1112	5	0.33803181250950587
1112	6	0.3141901514972645
1112	7	0.1993272098240766
1112	8	0.3456281560008634
1112	9	0.18280979338786096
1112	10	0.2999369116238455
1112	11	0.15633811633978212
1112	12	0.2508651835081963
1112	13	0.3641808551208072
1112	14	0.4199493358197728
1112	15	0.05334910480681998
1112	16	0.42483243612252153
1112	17	0.764071164430348
1112	18	0.4386249953849628
1112	19	0.48065785388644144
1112	20	0.32062524346542887
1112	21	0.3258992434649173
1112	22	0.22421960107016758
1112	23	0.5164913867907757
1112	24	0.42915949684278587
1112	25	0.4323643164615021
1113	1	-0.18499531234032318
1113	2	0.014343113164326127
1113	3	-0.02021100487069205
1113	4	0.11655400775446623
1113	5	0.010965094587930935
1113	6	-0.3709246720265398
1113	7	-0.35928650404327556
1113	8	-0.10078952070240632
1113	9	-0.370278207551614
1113	10	-0.14642828690786303
1113	11	-0.2787954690478003
1113	12	-0.41747506587321226
1113	13	-0.3287353256793395
1113	14	0.030093953550235142
1113	15	-0.31013388611867754
1113	16	-0.3492388516202496
1113	17	-0.1250353946988838
1113	18	-0.4463142281032404
1113	19	-0.6853037214495501
1113	20	-0.5366564239458995
1113	21	-0.1880269219495858
1113	22	-0.7511045571460144
1113	23	-4.431432743718403
1113	24	-2.0398491152655467
1113	25	-1.082464345060714
1114	1	-0.6133586732562044
1114	2	-0.9758345593544426
1114	3	-0.7548250249870954
1114	4	-0.6015421252554205
1114	5	-0.594667114668681
1114	6	-1.1846024835766897
1114	7	-1.2523665765313652
1114	8	-0.9843694349823657
1114	9	-1.2664332706910386
1114	10	-0.8856677373024269
1114	11	-0.9806019181862808
1114	12	-1.9805713427086644
1114	13	-1.1068968513809665
1114	14	-3.03787896888943
1114	15	-1.7657819161878123
1114	16	-1.7632119850040837
1114	17	-1.3827257478385917
1114	18	-1.4622165417772166
1114	19	-1.632055660697631
1114	20	-1.162632280427161
1114	21	-1.015300085057369
1114	22	-1.2183775284863714
1114	23	-5.4716291135610575
1114	24	-2.658420831488108
1114	25	-2.633435167647079
1115	1	-1.5854390167649157
1115	2	-1.546546325102181
1115	3	-1.2832645399773064
1115	4	-1.3552167972323659
1115	5	-1.2236346259059552
1115	6	-1.3765599949575709
1115	7	-1.842555803110205
1115	8	-2.340838226718244
1115	9	-3.6865426037307714
1115	10	-4.545108243183144
1115	11	-3.0287989282818764
1115	12	-3.271812253562029
1115	13	-2.5771069488903358
1115	14	-3.21132645408591
1115	15	-2.829311879855158
1115	16	-2.917128395969936
1115	17	-2.8604441147361506
1115	18	-5.4622802076891475
1115	19	-3.560857544825673
1115	20	-3.9119302799771916
1115	21	-3.6817237362256137
1115	22	-3.1307674893773125
1115	23	-3.3889261365813113
1115	24	-3.1121572546127183
1115	25	-3.377626490892794
1116	1	-1.9956619683560461
1116	2	-1.7834787018367315
1116	3	-2.373870403598172
1116	4	-2.1369110777792883
1116	5	-2.259698805495546
1116	6	-4.712854783182472
1116	7	-3.092690200187768
1116	8	-3.5188394789640114
1116	9	-4.687989665439769
1116	10	-3.93046165365854
1116	11	-3.365437784989467
1116	12	-3.184157442685038
1116	13	-4.561613996760823
1116	14	-7.49157885738083
1116	15	-5.538707711152927
1116	16	-4.275702191730247
1116	17	-5.594598719231872
1116	18	-4.194767311352365
1116	19	-3.8783613683627074
1116	20	-4.705925183257158
1116	21	-5.376605979260994
1116	22	-4.961657580882978
1116	23	-4.04717390975733
1116	24	-4.936182327444367
1116	25	-4.991099000309564
1201	1	0.5279126333951574
1201	2	0.604260271902902
1201	3	1.0097689908194738
1201	4	0.8721671698621876
1201	5	0.4570785218782163
1201	6	0.990696966083398
1201	7	1.0703567737509516
1201	8	1.0114835612503228
1201	9	1.4380952639587292
1201	10	1.3833704596986394
1201	11	1.3964742644215846
1201	12	1.253567195076272
1201	13	1.3244394360253509
1201	14	1.4359553245686811
1201	15	1.4257022654968665
1201	16	1.3160512600713048
1201	17	1.6192519628331397
1201	18	1.3433386336544828
1201	19	1.6088963078730458
1201	20	1.4254671422724954
1201	21	1.6855199612451202
1201	22	1.4791228110720311
1201	23	1.7738820265075503
1201	24	1.6572256111142636
1201	25	1.6938654298735827
1202	1	1.0515119336346395
1202	2	1.1030192754030317
1202	3	1.1568582117111497
1202	4	1.3016318982803412
1202	5	1.1940210726965503
1202	6	1.4497164846523134
1202	7	1.402734872945338
1202	8	1.3904098490215944
1202	9	1.6773544807495568
1202	10	1.4747859696446852
1202	11	1.4463391769143499
1202	12	1.5762685460328072
1202	13	1.5598258150131414
1202	14	2.010610278865208
1202	15	1.7743426989174296
1202	16	1.5238508871766214
1202	17	1.8038769871841254
1202	18	1.6792898740450681
1202	19	1.8858465226963474
1202	20	1.6663971447338142
1202	21	1.7843632219564256
1202	22	1.6771319416860848
1202	23	1.851135420650543
1202	24	1.8603295840444218
1202	25	2.256640972797456
1203	1	0.9114059601901303
1203	2	1.0367258460794702
1203	3	0.9581469959518181
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2211	20	0.0910389095066293
2211	21	0.3610165252165132
2211	22	0.3712984466086766
2211	23	0.5054526487364512
2211	24	0.179424977350223
2211	25	0.5141921898936985
2212	1	0.21702930188770964
2212	2	0.28762455657687624
2212	3	-0.05674284847507849
2212	4	0.24810403011336035
2212	5	0.06932894187342953
2212	6	-0.029409062924848683
2212	7	0.17099716229718354
2212	8	0.17479521303231138
2212	9	0.16000740304067307
2212	10	-0.22327548815736975
2212	11	-0.20472725173229378
2212	12	0.21379433613973475
2212	13	0.21495590120836994
2212	14	0.30986012486474124
2212	15	0.12403245110532168
2212	16	-0.045043156193777634
2212	17	0.21570186559107185
2212	18	0.36040251384917354
2212	19	0.12295999686724457
2212	20	0.31480208896068973
2212	21	0.45011605832922186
2212	22	0.5861434192118864
2212	23	0.5393613535330744
2212	24	0.47777423131030616
2212	25	0.5121086000271333
2213	1	-0.30495290750940285
2213	2	-0.1325875601954678
2213	3	-0.32324751377749195
2213	4	-0.19918930216982744
2213	5	-0.2224410105433705
2213	6	0.005233784572830302
2213	7	-0.26674810166124274
2213	8	-0.07954109468597181
2213	9	-0.3649749745119024
2213	10	-0.01077958971084314
2213	11	-0.27108128286655386
2213	12	-0.26547168174985813
2213	13	-0.22534462938938304
2213	14	-0.35875665580793736
2213	15	-0.253312821171491
2213	16	-0.22762065097103346
2213	17	-0.2781338300582803
2213	18	-0.26378603255274413
2213	19	-0.2556255042553133
2213	20	-0.042992547021320604
2213	21	-0.21652595431942245
2213	22	-0.5615193751742638
2213	23	-0.2033908121389203
2213	24	-0.01028273189244544
2213	25	0.05863181418464336
2214	1	-3.1597274723651765
2214	2	-1.9033517060229705
2214	3	-2.106738069234691
2214	4	-3.848402739085021
2214	5	-2.253011695613637
2214	6	-1.9878122062317232
2214	7	-1.7808409088754331
2214	8	-1.558327732475714
2214	9	-1.374679203610377
2214	10	-1.3546123361387004
2214	11	-1.4185166748888918
2214	12	-1.4003779198335309
2214	13	-1.7573956948348552
2214	14	-1.6541753081481971
2214	15	-2.0395193246791123
2214	16	-1.8296452125295843
2214	17	-3.7903572809970143
2214	18	-3.7792378727538782
2214	19	-2.649470895649017
2214	20	-2.3863469315513157
2214	21	-2.397175488718009
2214	22	-3.302856610877871
2214	23	-2.1065216132636877
2214	24	-3.4268431413756204
2214	25	-3.2517818326360235
2215	1	-2.0120941457389527
2215	2	-3.66115528723822
2215	3	-2.311464372566437
2215	4	-3.7135769122879445
2215	5	-2.3592794219400055
2215	6	-2.3244925263378384
2215	7	-2.4764546502370166
2215	8	-1.7192154351095503
2215	9	-4.248808508922085
2215	10	-2.5680894173395785
2215	11	-2.2897189650269887
2215	12	-2.2225052103475917
2215	13	-2.1807724682765333
2215	14	-1.8141526016985452
2215	15	-2.082762851922759
2215	16	-2.0414113147775197
2215	17	-2.3862603354218663
2215	18	-1.996483657317107
2215	19	-2.172890919470987
2215	20	-2.053917408065047
2215	21	-2.7682531865413864
2215	22	-2.4567960790009913
2215	23	-2.5107209739118943
2215	24	-2.460491442303848
2215	25	-3.4644467318495025
2216	1	-3.8916100467812953
2216	2	-3.8661871024433765
2216	3	-5.253665624336254
2216	4	-5.825826567007397
2216	5	-5.534723231413832
2216	6	-4.524463506950031
2216	7	-6.290103294140184
2216	8	-4.090486279770541
2216	9	-4.852002339523725
2216	10	-3.811705827340308
2216	11	-4.18432842079384
2216	12	-4.541664064869
2216	13	-4.102476462031191
2216	14	-5.175683042534686
2216	15	-4.596315523462263
2216	16	-4.7912281105971815
2216	17	-4.54447349748936
2216	18	-4.064357188355313
2216	19	-4.780390193021553
2216	20	-5.3312621032090455
2216	21	-5.0486937332635655
2216	22	-4.718212526124926
2216	23	-4.876811742080559
2216	24	-5.209043077712288
2216	25	-5.060585030842585
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101965.0
3	1.0
4	897.0
5	5649.0
6	6970.0
7	6464.0
8	5829.0
9	5168.0
10	4679.0
11	4357.0
12	4211.0
13	3925.0
14	3914.0
15	4094.0
16	4311.0
17	4351.0
18	4996.0
19	6056.0
20	7862.0
21	10300.0
22	12934.0
23	15391.0
24	17877.0
25	19997.0
26	22448.0
27	24479.0
28	27703.0
29	32703.0
30	39639.0
31	48906.0
32	61126.0
33	77523.0
34	100285.0
35	132117.0
36	179128.0
37	275912.0
38	780210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.64432130621355	18.457595642066615	12.725219916121059	24.17286313559878
2	15.104929931251384	19.830567676281266	41.75789305910753	23.306609333359816
3	19.53376723726568	25.18233411894652	28.72268016015851	26.56121848362929
4	12.458052248476994	16.021122240120146	37.62820497293107	33.89262053847178
5	13.22004994804144	38.76098820577043	34.460176005077166	13.558785841110968
6	32.04198091186171	35.85286998357045	18.219054493837586	13.886094610730254
7	29.007733876687496	31.632685215753973	21.111112727082336	18.24846818047619
8	26.081436966627926	36.159929552092315	20.30769559016852	17.450937891111238
9	28.152088755124442	14.422077468749151	19.78950522237262	37.636328553753785
10	16.780371772301468	28.27031673712327	32.88020988131388	22.069101609261384
11	34.66743075342542	22.17912113538638	24.417711104420505	18.735737006767692
12	24.766626130503173	24.45067679876801	30.551029987063338	20.231667083665478
13	29.727227067080115	21.884714360236355	25.390465164534476	22.997593408149058
14	21.586262969856556	22.575077415516848	26.976331966269285	28.862327648357315
15	23.611371025372264	31.25252709383966	22.906111858263607	22.229990022524465
16	22.27325286835273	27.643866100712938	27.79663482166882	22.28624620926551
17	21.72558306652333	28.62894329663211	27.353831517667647	22.29164211917691
18	21.546391502629366	26.70712340922811	30.17196944166301	21.57451564647951
19	23.996006621367	26.279204854117555	27.52783873654853	22.196949787966915
20	24.739082433223285	26.33021290873449	27.878384412972444	21.05232024506978
21	23.895450616395156	25.952104964369845	26.76292796061897	23.38951645861603
22	24.318544832461967	27.7741895127621	26.607082071379317	21.300183583396613
23	22.699891002044197	27.378948266699766	27.863038831976354	22.05812189927968
24	23.845070026462952	26.052578756134526	28.38255985679557	21.71979136060695
25	23.009186375248422	26.904244389484468	28.169886124243703	21.916683111023406
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	289.0
1	289.0
2	659.0
3	1029.0
4	1029.0
5	1029.0
6	1800.0
7	2571.0
8	2571.0
9	2571.0
10	3011.5
11	3452.0
12	3452.0
13	3452.0
14	4961.5
15	6471.0
16	6471.0
17	6471.0
18	12678.5
19	18886.0
20	18886.0
21	18886.0
22	31626.5
23	44367.0
24	44367.0
25	44367.0
26	67531.5
27	90696.0
28	90696.0
29	90696.0
30	119351.5
31	148007.0
32	148007.0
33	148007.0
34	168740.0
35	189473.0
36	189473.0
37	189473.0
38	202641.0
39	215809.0
40	215809.0
41	215809.0
42	230436.5
43	245064.0
44	245064.0
45	245064.0
46	261888.5
47	278713.0
48	278713.0
49	278713.0
50	269889.0
51	261065.0
52	261065.0
53	261065.0
54	232294.5
55	203524.0
56	203524.0
57	203524.0
58	177973.5
59	152423.0
60	152423.0
61	152423.0
62	127133.5
63	101844.0
64	101844.0
65	101844.0
66	78390.5
67	54937.0
68	54937.0
69	54937.0
70	41188.5
71	27440.0
72	27440.0
73	27440.0
74	19587.0
75	11734.0
76	11734.0
77	11734.0
78	8105.5
79	4477.0
80	4477.0
81	4477.0
82	2999.5
83	1522.0
84	1522.0
85	1522.0
86	972.0
87	422.0
88	422.0
89	422.0
90	275.0
91	128.0
92	128.0
93	128.0
94	76.5
95	25.0
96	25.0
97	25.0
98	17.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.333659985554964
2	0.009203745246144479
3	0.008331811485983423
4	0.009785034419585182
5	0.011238257353186943
6	0.07895844605902895
7	0.07915220911684251
8	0.10385699898807244
9	0.08297902950866048
10	0.09097175564347015
11	0.08641832378485131
12	0.09789878496030521
13	0.08278526645084691
14	0.08748402060282594
15	0.08385096326882154
16	0.08632144225594453
17	0.0992066856005468
18	0.10133807923649604
19	0.09508922062200847
20	0.09416884609739404
21	0.08031478746372392
22	0.10167716458766979
23	0.09460481297747457
24	0.09063267029229641
25	0.09039046647002946
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2064377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.19131629679703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65273881725608	37.703806489195365
2	14.1664177881738	14.503951475739532
3	5.134470936335112	7.8852097715592695
4	2.408881593940166	4.932552783876944
5	1.3269999578009477	3.3965437282812307
6	0.7729685005905471	2.374156500071498
7	0.5229508375106702	1.8739379211478537
8	0.36016254611377035	1.474975585309581
9	0.2569764714051555	1.1839467445682519
>10	1.2590158038176826	11.265690314095064
>50	0.08024072876847349	2.8703529208082634
>100	0.04938990724905118	4.864172293851936
>500	0.0053480675886282076	1.928742194420581
>1k	0.003247041035952838	3.1066574948456394
>5k	1.910024138795787E-4	0.635303782228922
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7494	0.3630150888137196	No Hit
GTCCTACAGTGGACATTTCTAAATT	5501	0.2664726452581093	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4909	0.23779571270170127	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4412	0.21372065276836547	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4309	0.20873125402966608	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4288	0.20771399797614487	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4065	0.19691170750303846	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2855	0.13829838251443413	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2776	0.13447156212261616	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2122	0.10279130217009781	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2082	0.10085367159196212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	4.844076445339199E-5
5	0.0	0.0	0.0	0.0	4.844076445339199E-5
6	0.0	0.0	0.0	0.0	4.844076445339199E-5
7	0.0	0.0	0.0	0.0	4.844076445339199E-5
8	0.0	0.0	0.0	0.0	9.688152890678398E-5
9	0.0	0.0	0.0	0.0	9.688152890678398E-5
10	0.0	0.0	0.0	0.0	9.688152890678398E-5
11	0.0	0.0	0.0	0.0	9.688152890678398E-5
12	0.0	0.0	0.0	0.0	1.4532229336017597E-4
13	0.0	0.0	0.0	0.0	1.4532229336017597E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1860	0.0	13.00502	1
TAGGACC	870	0.0	12.992097	4
ACTAGAC	100	1.446424E-7	12.347951	3
CCAACGA	85	3.9534734E-6	12.29178	19
GTATAGG	195	0.0	12.209437	1
CGCGCCT	210	0.0	11.760809	12
GTCCTAT	220	0.0	11.68776	1
TCCTATA	270	0.0	11.609185	2
GCGCCAC	205	0.0	11.583724	13
GTTCCGA	75	2.0276527E-4	11.428033	1
GTCCTAC	2870	0.0	11.28203	1
ACTGTTC	385	0.0	11.102862	8
TGTAGGA	2560	0.0	11.093863	2
GTGCGCC	200	0.0	10.9237175	11
ATTATAC	235	0.0	10.913083	3
CGTGCGC	175	2.0008883E-11	10.855868	10
GTATCAA	4080	0.0	10.830489	1
GCGTGCG	185	5.456968E-12	10.782517	9
ACCGTGC	80	3.775049E-4	10.686504	8
GTAGGAC	2535	0.0	10.678702	3
>>END_MODULE