FastQCFastQC Report
Thu 26 May 2016
SRR1512835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064377
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92600.4485614788384098No Hit
TATCAACGCAGAGTACTTTTTTTTT67130.32518285177562045No Hit
GGTATCAACGCAGAGTACTTTTTTT55020.26652108602256275No Hit
GTCCTACAGTGGACATTTCTAAATT51400.24898552929043483No Hit
CTTTAGGACGTGAAATATGGCGAGG45790.22181026043208196No Hit
GTCCTAAAGTGTGTATTTCTCATTT44520.21565828334650117No Hit
CTGTAGGACGTGGAATATGGCAAGA43210.2093125432031068No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31640.15326657873053226No Hit
GTCCTACAGTGTGCATTTCTCATTT27330.1323886092511203No Hit
GTACTGGTTCACTATCGGTCAGTCA25670.12434744235185725No Hit
CTGAAGGACCTGGAATATGGCGAGA22530.10913704231349215No Hit
CTGTAGGACCTGGAATATGGCGAGA21520.10424452510369955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCCT400.00530886314.23824515
CCGTAAA500.001485661813.31677152
GGTATCA20650.013.2706111
TAGGACC9750.013.1691534
GGCGAGG12400.012.32360719
ATAGGAC2100.012.2296883
AACGAAG1251.4297257E-912.14997115
CTGCGCG658.0434297E-411.6891779
GACGTGA15650.011.4797867
ACGAAGG1251.8559149E-811.39032216
TTAGGAC19250.011.2661353
TAGGACT4100.011.1348944
TGGCGAG28100.011.01172818
GTGCACG951.3728153E-510.99119311
TGTAGGA27550.010.9448182
TAGGACA7100.010.850644
TAAAACG1408.463758E-910.84818712
AGGACCT18050.010.6966445
ACCGTGC803.771848E-410.6874868
TAGAAAT7050.010.6577784
Produced by FastQC (version 0.11.5)