##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512835_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2064377
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18680793285335	31.0	30.0	34.0	26.0	34.0
2	30.217032547834044	31.0	30.0	34.0	26.0	34.0
3	30.391654237573853	31.0	30.0	34.0	26.0	34.0
4	33.975341713262644	37.0	35.0	37.0	30.0	37.0
5	33.5478393723627	37.0	35.0	37.0	28.0	37.0
6	33.401272151356075	37.0	35.0	37.0	27.0	37.0
7	33.23710349417766	37.0	35.0	37.0	27.0	37.0
8	33.177567857033864	37.0	35.0	37.0	26.0	37.0
9	34.49410548557749	39.0	35.0	39.0	26.0	39.0
10	34.42322308376813	39.0	35.0	39.0	26.0	39.0
11	34.393172371131826	39.0	35.0	39.0	26.0	39.0
12	34.25517141491113	39.0	35.0	39.0	25.0	39.0
13	34.243033612562044	39.0	35.0	39.0	25.0	39.0
14	35.30441096757036	40.0	35.0	41.0	25.0	41.0
15	35.299955870463585	40.0	36.0	41.0	25.0	41.0
16	35.300014968196216	40.0	36.0	41.0	25.0	41.0
17	35.296845004570386	40.0	36.0	41.0	25.0	41.0
18	35.222382345860275	40.0	35.0	41.0	25.0	41.0
19	35.2365023442908	40.0	35.0	41.0	25.0	41.0
20	35.20918029991615	40.0	35.0	41.0	25.0	41.0
21	35.17608992931039	40.0	35.0	41.0	25.0	41.0
22	35.13681076663807	40.0	35.0	41.0	25.0	41.0
23	34.973267479728754	40.0	35.0	41.0	24.0	41.0
24	34.9279249865698	40.0	35.0	41.0	23.0	41.0
25	34.86944729572166	40.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6708735848124441
1101	2	0.5526093330499542
1101	3	0.4642036814708632
1101	4	0.545714428603759
1101	5	0.6501687712546769
1101	6	0.6718522514704262
1101	7	0.7350432814645416
1101	8	0.4755642394014288
1101	9	0.907618741939757
1101	10	0.49618999513921125
1101	11	0.7352014063498302
1101	12	0.8322385954349087
1101	13	0.8986795246288324
1101	14	1.0815728732137941
1101	15	0.7243045936568677
1101	16	0.8891515797457075
1101	17	1.0067017172736925
1101	18	0.9775082333830767
1101	19	0.9010469052472629
1101	20	0.865558268024671
1101	21	0.8431861756300094
1101	22	0.979256659250666
1101	23	0.9913387941470759
1101	24	0.9946357496631109
1101	25	1.0174516719581845
1102	1	0.7914920805166403
1102	2	0.9322305549060133
1102	3	0.5788662429150087
1102	4	0.7124598489614087
1102	5	0.8645165280350824
1102	6	0.8171523030330832
1102	7	0.742134163495038
1102	8	0.839701516437664
1102	9	0.9606759236257432
1102	10	0.7046243121210551
1102	11	0.9317473385929205
1102	12	0.9463431375032059
1102	13	1.036892048986907
1102	14	0.9701422290546873
1102	15	1.0387496810531331
1102	16	1.092793477088847
1102	17	1.0658848110656791
1102	18	0.38635303872997895
1102	19	0.7198793933000118
1102	20	0.8951232304668864
1102	21	0.8958379316882201
1102	22	0.683503950177915
1102	23	0.8878624422132262
1102	24	0.8759052076533678
1102	25	1.0926878110374716
1103	1	0.8168461301955041
1103	2	0.9894375098642278
1103	3	0.7640316923183263
1103	4	0.5845945715608067
1103	5	0.7065588158240175
1103	6	0.7523019449957857
1103	7	0.853083753197005
1103	8	0.8539774355355263
1103	9	0.9229991725189066
1103	10	0.9079061242130493
1103	11	1.1175378847274189
1103	12	1.1079821831540073
1103	13	1.1852814508106633
1103	14	1.147763016396425
1103	15	1.173893483387232
1103	16	1.2900336409890727
1103	17	1.2070882145038198
1103	18	1.2664676335500076
1103	19	1.1850713104254282
1103	20	1.105059457184943
1103	21	1.107264250124942
1103	22	1.1323918609728594
1103	23	1.177324447685713
1103	24	1.2224741447156902
1103	25	1.1905596905722504
1104	1	0.849027629221645
1104	2	0.8972193647708089
1104	3	0.8230599463400168
1104	4	0.7213488457330186
1104	5	0.8792155595179594
1104	6	0.7788655345790687
1104	7	0.7105972069205251
1104	8	0.78311856136812
1104	9	0.9140527229072433
1104	10	0.9704723107617355
1104	11	1.1840330875552283
1104	12	1.139889269503108
1104	13	0.9099947506399388
1104	14	1.1836696218260059
1104	15	1.1747120084070701
1104	16	1.203631159444356
1104	17	1.0850282438399077
1104	18	0.7422649740650726
1104	19	1.10781851508073
1104	20	1.1152715907843813
1104	21	1.0953925594523781
1104	22	1.0564822738422635
1104	23	1.0775455744801405
1104	24	1.2956036322959434
1104	25	1.1316566679930773
1105	1	0.5489457433655645
1105	2	0.5484451712224221
1105	3	0.5864544872829427
1105	4	0.3949220467255756
1105	5	0.44746866125493057
1105	6	0.5147836487229895
1105	7	0.4901803334713932
1105	8	0.5386446159586953
1105	9	0.7941743109965742
1105	10	0.47640283185354804
1105	11	0.6024350726668857
1105	12	0.7444674332748207
1105	13	0.7905456191262914
1105	14	0.9399673885753899
1105	15	0.8970817354542149
1105	16	0.8802564696180539
1105	17	0.632924025477628
1105	18	0.5652897879608574
1105	19	0.8209773240137324
1105	20	0.909569357533762
1105	21	0.6449012443158963
1105	22	0.7107949289291113
1105	23	0.846034408227041
1105	24	0.7243033841569826
1105	25	0.6807261469012644
1106	1	-0.07457988137799276
1106	2	0.24284504617601854
1106	3	-1.574434905265072
1106	4	-0.4204561209538369
1106	5	-0.1740539740980367
1106	6	-0.12561659746211262
1106	7	0.16077528081513748
1106	8	0.1914916685758712
1106	9	0.24171353453656508
1106	10	0.10628091160970143
1106	11	0.19948554061397772
1106	12	0.3166790938519881
1106	13	0.35731395112448894
1106	14	0.32303727779208913
1106	15	0.394990627425841
1106	16	0.28768930602218745
1106	17	0.4120082559537792
1106	18	0.39847817818379383
1106	19	0.37607775190689097
1106	20	0.38693967073592717
1106	21	0.2733719658727267
1106	22	0.2369522920130649
1106	23	0.2607751323238716
1106	24	0.35412089456123397
1106	25	0.3214810168725464
1107	1	0.822692798489463
1107	2	0.9038216895982991
1107	3	0.8039883953744251
1107	4	0.7202234810314962
1107	5	0.7589282441842826
1107	6	0.7933933908287827
1107	7	0.711950978976283
1107	8	0.7672482330209718
1107	9	0.9390045128391691
1107	10	0.9334390129337322
1107	11	1.0084343286981863
1107	12	1.2132982446339398
1107	13	1.0936826124470045
1107	14	1.200505077760802
1107	15	1.1090861111255421
1107	16	1.264302673360902
1107	17	1.1771188287516878
1107	18	1.4097370251927543
1107	19	1.2183621171739958
1107	20	1.1575219496969922
1107	21	1.1293115146693466
1107	22	1.130692805032929
1107	23	1.3175565039480261
1107	24	1.3969881550579473
1107	25	1.3042079577629124
1108	1	0.5406490465592633
1108	2	0.49268773405791677
1108	3	0.48604945362857777
1108	4	0.49544804528233044
1108	5	0.608702003498145
1108	6	0.49940965326172915
1108	7	0.5087926483420873
1108	8	0.5076316948626385
1108	9	0.7318994401204577
1108	10	0.6795021457421342
1108	11	0.6184236417675706
1108	12	0.6636760350719939
1108	13	0.772049725875263
1108	14	0.8172438571725991
1108	15	0.8459422612180347
1108	16	0.7645348940984746
1108	17	0.8434114742805079
1108	18	0.9902443172729818
1108	19	0.9941351743140814
1108	20	0.9522820834958239
1108	21	0.9449896111647789
1108	22	1.0736695699151255
1108	23	1.164017535232233
1108	24	1.0748757620581557
1108	25	1.0171710925371045
1109	1	0.2924723949821981
1109	2	0.23996777425606197
1109	3	0.3051790903538958
1109	4	0.3223205056520513
1109	5	-0.020594735450231383
1109	6	0.2329199012650136
1109	7	0.3273444596677777
1109	8	0.24369461689154548
1109	9	0.24087285014842763
1109	10	0.03236936107983723
1109	11	0.3142401436570168
1109	12	0.5999471064030502
1109	13	0.31533656671859234
1109	14	0.4938974992117835
1109	15	0.4139948308477841
1109	16	0.4908839066971069
1109	17	0.6311450743777911
1109	18	0.8019711626133699
1109	19	0.6254957851790479
1109	20	0.6910471836126035
1109	21	0.715517487156248
1109	22	0.6784394745363045
1109	23	0.6180707335316171
1109	24	0.6809292540426171
1109	25	0.5266364250780953
1110	1	0.20805296316913413
1110	2	0.03409545873672215
1110	3	0.23894479203324437
1110	4	0.1300545113249143
1110	5	0.2165679316966731
1110	6	0.1956550325062949
1110	7	0.16287910466350297
1110	8	0.24093247200838164
1110	9	0.193277964731287
1110	10	0.4066906433273729
1110	11	0.2955079839163517
1110	12	0.3595990806500424
1110	13	0.2817054830213266
1110	14	0.426905918463504
1110	15	0.3156516675503198
1110	16	0.31049896230018703
1110	17	0.3905390683447436
1110	18	0.2609558999057029
1110	19	0.33342699043770807
1110	20	0.3814226732348658
1110	21	0.45530227847586957
1110	22	0.42905988784502824
1110	23	0.4571549710715743
1110	24	0.551366875041218
1110	25	0.42470134786410085
1111	1	0.1900888913128469
1111	2	0.041281087479237755
1111	3	0.0862501812548011
1111	4	0.2968209783907838
1111	5	0.3854302071457809
1111	6	0.5130203019673729
1111	7	0.33084317651979234
1111	8	0.4032079211101731
1111	9	0.5181282641324856
1111	10	0.39681040380641264
1111	11	0.42275349289838715
1111	12	0.6458266255602254
1111	13	0.5703282375123493
1111	14	0.3622352597808671
1111	15	0.44469358371798506
1111	16	0.38445105810856717
1111	17	0.577964218045345
1111	18	0.3585606903248646
1111	19	0.17264854732393076
1111	20	0.4371112959893537
1111	21	0.4400328173980199
1111	22	0.18504791179712043
1111	23	0.33200527047277006
1111	24	0.41873214450230023
1111	25	0.4336833837922498
1112	1	0.10067303543028672
1112	2	-0.2456069836468444
1112	3	0.0679856237413965
1112	4	0.10056823787121516
1112	5	-0.021107457036876554
1112	6	-0.09591446960026673
1112	7	0.02690722108384591
1112	8	0.14528847476290707
1112	9	0.005670173974138493
1112	10	-0.23644635463565322
1112	11	-0.09884397018932134
1112	12	0.16324620836864767
1112	13	0.17789686120670467
1112	14	-0.008848802098391673
1112	15	-0.27831638788725144
1112	16	-0.24494809709747045
1112	17	-0.09541718094357776
1112	18	0.06155244301025675
1112	19	0.023626729805933167
1112	20	-0.09116932959918955
1112	21	-0.10051178009579331
1112	22	0.047754975785657905
1112	23	0.28748423105447785
1112	24	0.31328381990187637
1112	25	0.2677684885600726
1113	1	-0.6275148070654737
1113	2	-0.5298098613222457
1113	3	-0.44289001858867394
1113	4	-2.0908325898526705
1113	5	-0.716528186098131
1113	6	-0.4512490317057072
1113	7	-0.3986737774142881
1113	8	-0.33122139654630445
1113	9	-0.2407108964521214
1113	10	-0.3810684888641731
1113	11	-0.6189961385378311
1113	12	-0.3186611162813193
1113	13	-0.3583924366408695
1113	14	-0.4752754331190445
1113	15	-0.5895246060423105
1113	16	-0.7365325066767596
1113	17	-0.8135997664476733
1113	18	-0.7305580327153791
1113	19	-0.4483068064063005
1113	20	-0.21999424975805226
1113	21	-0.3143881279453353
1113	22	-0.33528952882315366
1113	23	-0.20207004394399064
1113	24	-0.05329886945762041
1113	25	-0.3688810921191532
1114	1	-0.721114569273869
1114	2	-1.8823224940013326
1114	3	-1.3239587384452314
1114	4	-0.635069499328857
1114	5	-0.5434993155123777
1114	6	-0.3031048609559903
1114	7	-0.3285185416841827
1114	8	-0.4287562263047704
1114	9	-0.6615483447947668
1114	10	-0.787212717144115
1114	11	-0.6636223358856839
1114	12	-0.6713846931273224
1114	13	-0.7212394158245132
1114	14	-0.7494980559221105
1114	15	-0.9610365963005307
1114	16	-1.2978587701843765
1114	17	-1.2465262332287637
1114	18	-1.0425377437218657
1114	19	-1.2819907545140339
1114	20	-0.7642081471433073
1114	21	-0.9347787581889762
1114	22	-0.8353604827702128
1114	23	-0.6956813121978485
1114	24	-0.6069899129600671
1114	25	-1.3192775712692395
1115	1	-0.8583859344529223
1115	2	-1.0161089882115633
1115	3	-0.5626676128989239
1115	4	-0.3552707410534879
1115	5	-0.6431278512442731
1115	6	-0.6475264346050054
1115	7	-0.5738304807180867
1115	8	-0.8927390317216393
1115	9	-1.808009142389821
1115	10	-1.4840088378317091
1115	11	-1.6019568739248697
1115	12	-2.2102122829656636
1115	13	-2.2911412362866415
1115	14	-1.8482864888311639
1115	15	-2.181169863893622
1115	16	-1.8930397872114142
1115	17	-1.78729252188905
1115	18	-2.1100221742949117
1115	19	-2.4303404670600486
1115	20	-2.715541062419774
1115	21	-1.9020128333338988
1115	22	-1.9124996334765214
1115	23	-1.8981910894628982
1115	24	-2.2548896559029643
1115	25	-2.1550247840905
1116	1	-1.6305233664318486
1116	2	-1.6651263342082672
1116	3	-1.8181774942745612
1116	4	-1.494010499258387
1116	5	-1.8666667815312685
1116	6	-2.5297921021337544
1116	7	-2.0764813334615617
1116	8	-2.4959159074482145
1116	9	-3.5237635107933514
1116	10	-2.335972650321864
1116	11	-3.2826963170743113
1116	12	-5.408567214879788
1116	13	-4.4280905622075295
1116	14	-3.749153788666259
1116	15	-4.769623380251581
1116	16	-4.268588591894975
1116	17	-4.122723499817223
1116	18	-3.9001211575431327
1116	19	-4.315913618751274
1116	20	-6.156762979113527
1116	21	-5.003352816065668
1116	22	-4.133831281196652
1116	23	-4.834143902928126
1116	24	-4.71765867438555
1116	25	-3.914446637374354
1201	1	0.4907625197762151
1201	2	0.7498586555544833
1201	3	0.7062263183299535
1201	4	0.5539757849964104
1201	5	0.18041744212870725
1201	6	0.5151829885721497
1201	7	0.7795333326271319
1201	8	0.7784789717573446
1201	9	0.551182388967959
1201	10	0.7547604796986533
1201	11	0.597901254998014
1201	12	0.9718458399640468
1201	13	0.9326963468747138
1201	14	0.9856088105104206
1201	15	0.8276133705606128
1201	16	0.7167526575642356
1201	17	0.5425571429110576
1201	18	0.8841524656605628
1201	19	0.9630873847672063
1201	20	0.9548310200246135
1201	21	0.6554340964197749
1201	22	0.7262427639187123
1201	23	0.8583046949323645
1201	24	0.5969160809350313
1201	25	0.876572871892769
1202	1	0.8642725780444458
1202	2	0.888716352212434
1202	3	0.8885384954725453
1202	4	0.7641489522009479
1202	5	0.8726630393737409
1202	6	0.9797342160705895
1202	7	1.1099571411039904
1202	8	1.0636365762252353
1202	9	1.2313555999609846
1202	10	1.2352207802852888
1202	11	1.1754659038738566
1202	12	1.2706676952296334
1202	13	1.2483524344785906
1202	14	1.3672491703052074
1202	15	1.2865555973440834
1202	16	1.4898755033357602
1202	17	1.4466869173463337
1202	18	1.4348469664671342
1202	19	1.5052572839243368
1202	20	1.2487549793681154
1202	21	1.4524440754104688
1202	22	1.3252143860207397
1202	23	1.5470832617064119
1202	24	1.5176871128292504
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2211	9	0.06512675775849885
2211	10	-0.048608270734369796
2211	11	-0.038744037697490796
2211	12	-0.04624917278051299
2211	13	0.16972306487888744
2211	14	0.005495172995736652
2211	15	0.060743586341025946
2211	16	0.037122061533509054
2211	17	-0.0828202205867754
2211	18	-0.06507439245076085
2211	19	-0.08677453392283496
2211	20	0.0052068945294507785
2211	21	0.15038033263038386
2211	22	0.07567953965529739
2211	23	-0.1435862248844515
2211	24	0.18347924604389476
2211	25	-0.06567172326522552
2212	1	-0.13198982667001147
2212	2	-0.020916205415932865
2212	3	0.04402257433036283
2212	4	-0.031114895216859395
2212	5	-0.28740718234572427
2212	6	-0.02553241288924113
2212	7	-0.17485474837364734
2212	8	0.04126744020783235
2212	9	-0.08026410738399647
2212	10	-0.24026317976053946
2212	11	0.0716520102103928
2212	12	0.3251176019382669
2212	13	0.2599962970679215
2212	14	0.34400571217489073
2212	15	0.17589104259249666
2212	16	0.1966895722469033
2212	17	0.06981317319626612
2212	18	-0.06553577103204589
2212	19	0.2618123762302389
2212	20	0.16585937400329698
2212	21	0.21650214496281706
2212	22	0.2935469660796599
2212	23	-0.21297276006377786
2212	24	-0.1462204966667784
2212	25	-0.05901790263884976
2213	1	-0.566706692681354
2213	2	-0.12679175663790332
2213	3	-0.26480165390712784
2213	4	-0.5598374378629032
2213	5	-0.2760133561446949
2213	6	-0.15956511108048943
2213	7	-0.6322548165580031
2213	8	-0.7697365513906078
2213	9	-0.5369031003859277
2213	10	-0.40701919371772277
2213	11	-0.3292619387036524
2213	12	-0.6917202454798996
2213	13	-0.4128510942781958
2213	14	-0.6880782929098572
2213	15	-0.7842129220841301
2213	16	-1.377394481581284
2213	17	-1.0027975207569426
2213	18	-0.5387927954746203
2213	19	-0.6128759551077181
2213	20	-0.5504967132007863
2213	21	-0.40404889868787564
2213	22	-0.609179758904105
2213	23	-0.7577018768636989
2213	24	-0.5556277547908692
2213	25	-0.57961920472858
2214	1	-1.3373714886439743
2214	2	-0.7677777347329915
2214	3	-0.8116627806058325
2214	4	-0.9963969173851339
2214	5	-1.2655579380969257
2214	6	-1.7654617180996937
2214	7	-1.2590384811682611
2214	8	-1.1270924317521889
2214	9	-0.9009748198211369
2214	10	-1.2548444665712495
2214	11	-1.2617318523973111
2214	12	-0.9912975368348356
2214	13	-1.3722914156711568
2214	14	-1.046806395145488
2214	15	-1.1880810975209712
2214	16	-1.2072080667703773
2214	17	-1.2933300273684694
2214	18	-1.2694123756964117
2214	19	-1.3337618478408615
2214	20	-1.5948171500167305
2214	21	-1.6514342018200097
2214	22	-2.1201763545346566
2214	23	-2.201370359072129
2214	24	-1.824656117925791
2214	25	-2.252033434363277
2215	1	-1.3997490260107242
2215	2	-0.8457115125375871
2215	3	-0.9536001730152783
2215	4	-0.9656536447514483
2215	5	-1.502030058639086
2215	6	-1.3311668887093049
2215	7	-1.691510588765766
2215	8	-1.5207626472217015
2215	9	-1.5122464171707577
2215	10	-1.542154915402449
2215	11	-1.1854781196248894
2215	12	-1.5101957828910457
2215	13	-1.605153785613055
2215	14	-1.7776491131958423
2215	15	-1.77886254166107
2215	16	-1.8221730790782686
2215	17	-1.8168323830103361
2215	18	-1.5624242792594885
2215	19	-1.8004256463778532
2215	20	-1.7745313320105964
2215	21	-1.4431251793756843
2215	22	-1.567878611454482
2215	23	-1.9598891325355083
2215	24	-1.8747487007711143
2215	25	-1.9844891898143544
2216	1	-1.6371594546988568
2216	2	-1.0347942413324986
2216	3	-1.0607845553364506
2216	4	-1.2900657030788736
2216	5	-2.128777637430389
2216	6	-2.526610922002881
2216	7	-2.2736879381302515
2216	8	-2.4552974938337826
2216	9	-4.153552505723884
2216	10	-2.35590312050315
2216	11	-2.753986835667476
2216	12	-3.1134826144562666
2216	13	-2.9202051618922553
2216	14	-3.0311909642082995
2216	15	-3.09549937212649
2216	16	-2.4251778205838264
2216	17	-2.6883700748344665
2216	18	-3.1484696583271656
2216	19	-3.368944009823302
2216	20	-2.596904122321213
2216	21	-3.3119432683332306
2216	22	-2.6717074341124487
2216	23	-3.8185490851243102
2216	24	-3.241172304338491
2216	25	-3.3871634884771105
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59922.0
3	0.0
4	1003.0
5	8639.0
6	10771.0
7	8405.0
8	6639.0
9	5677.0
10	4793.0
11	4253.0
12	3876.0
13	3676.0
14	3628.0
15	3584.0
16	3634.0
17	3815.0
18	4194.0
19	5042.0
20	6660.0
21	8767.0
22	10993.0
23	13420.0
24	15458.0
25	17832.0
26	19834.0
27	21548.0
28	24527.0
29	28963.0
30	35992.0
31	46067.0
32	58527.0
33	74949.0
34	97413.0
35	128621.0
36	175501.0
37	272357.0
38	865397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.39547556943246	19.431294786141628	13.818954901143229	26.354274743282684
2	13.482023822850802	20.329627459617996	40.616656998537906	25.571691718993293
3	17.480976241256048	25.16087709387315	29.633894994084297	27.724251670786504
4	11.592321327272737	16.669290686312742	36.8791670441218	34.85922094229272
5	12.830164180326559	38.956139284141415	34.50403122851574	13.709665307016289
6	32.207848985857204	36.342891597448784	17.749183174831366	13.700076241862646
7	28.564890921316266	31.842947638062668	21.147559818632843	18.44460162198822
8	26.0625696699462	36.550041196142104	19.873358212572093	17.5140309213396
9	27.48196161501445	14.371888221123562	19.724099437384567	38.42205072647742
10	16.18627007212249	28.897459283892772	32.82237280184392	22.09389784214082
11	34.459136391271386	22.131529929829068	24.384799284968597	19.024534393930953
12	24.285677608993353	24.76369292505361	30.556649513725503	20.393979952227532
13	29.445689723244524	21.868231471034633	25.603212028052923	23.082866777667917
14	21.327253667950675	22.509940088201326	27.144433550607257	29.01837269324074
15	23.51816906764983	31.333011964139075	22.83578437903478	22.31303458917632
16	22.23783243289332	27.617364023222823	27.681118276736093	22.463685267147763
17	21.46640229466885	28.72850988464164	27.456336716784634	22.348751103904878
18	21.42630841583295	26.592925669927897	30.333118243315255	21.6476476709239
19	23.798933504881965	26.193606454775686	27.762879226791036	22.24458081355131
20	24.45734813764894	26.28067970933266	28.099651561530415	21.162320591487987
21	23.513315168029063	25.825637299424763	27.12913109294581	23.531916439600366
22	24.016648194507955	27.6193004348062	27.01679157979585	21.347259790889996
23	22.33748971239265	27.275831885358148	28.158519495227857	22.228158907021346
24	23.707152327312308	25.98682314325339	28.482685090949957	21.823339438484346
25	22.638231910384498	26.930330003027553	28.42051468362095	22.010923402967002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	107.0
1	107.0
2	366.5
3	626.0
4	626.0
5	626.0
6	1435.0
7	2244.0
8	2244.0
9	2244.0
10	2776.0
11	3308.0
12	3308.0
13	3308.0
14	4671.0
15	6034.0
16	6034.0
17	6034.0
18	12606.5
19	19179.0
20	19179.0
21	19179.0
22	32681.5
23	46184.0
24	46184.0
25	46184.0
26	70800.0
27	95416.0
28	95416.0
29	95416.0
30	123368.0
31	151320.0
32	151320.0
33	151320.0
34	170784.0
35	190248.0
36	190248.0
37	190248.0
38	203403.0
39	216558.0
40	216558.0
41	216558.0
42	231144.0
43	245730.0
44	245730.0
45	245730.0
46	262871.0
47	280012.0
48	280012.0
49	280012.0
50	269558.0
51	259104.0
52	259104.0
53	259104.0
54	230733.5
55	202363.0
56	202363.0
57	202363.0
58	176341.5
59	150320.0
60	150320.0
61	150320.0
62	124915.5
63	99511.0
64	99511.0
65	99511.0
66	76327.0
67	53143.0
68	53143.0
69	53143.0
70	39574.5
71	26006.0
72	26006.0
73	26006.0
74	18469.5
75	10933.0
76	10933.0
77	10933.0
78	7564.5
79	4196.0
80	4196.0
81	4196.0
82	2784.0
83	1372.0
84	1372.0
85	1372.0
86	873.5
87	375.0
88	375.0
89	375.0
90	227.5
91	80.0
92	80.0
93	80.0
94	44.0
95	8.0
96	8.0
97	8.0
98	4.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0870480537227454
2	0.04340292495023923
3	0.018359049727835565
4	0.005522247147686688
5	0.017729319789941467
6	0.05841956193079074
7	0.07750522312542718
8	0.05217070331630317
9	0.021556140181759435
10	0.04567964087954865
11	0.0063941809078477425
12	3.875261156271359E-4
13	0.006587943965661312
14	4.3596688008052796E-4
15	0.014144703220390461
16	0.00973659365513179
17	0.005522247147686688
18	0.0060550955566739985
19	0.0021798344004026397
20	0.0011625783468814078
21	9.688152890678398E-5
22	4.3596688008052796E-4
23	0.0
24	0.0
25	9.688152890678398E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2064377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.07616008621729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.82248912795069	36.46670623448077
2	14.53379679495239	14.555934699291756
3	5.328048052078819	8.004245616088715
4	2.4833887725148114	4.974342949150654
5	1.3263311696656934	3.320878598975954
6	0.840536634382434	2.5254508236999036
7	0.5562886908630242	1.949976107646633
8	0.39948613850383385	1.6003785459154207
9	0.26597957829088026	1.1987312347942818
>10	1.3011112932409301	11.482097822041
>50	0.08245096875312725	2.8858355321409106
>100	0.05124633764026909	5.024504266531094
>500	0.005346750153583744	1.9655869860808854
>1k	0.0031108364529941757	2.7496895589516965
>5k	3.8885455662427196E-4	1.2956410242103855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9260	0.4485614788384098	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6713	0.32518285177562045	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5502	0.26652108602256275	No Hit
GTCCTACAGTGGACATTTCTAAATT	5140	0.24898552929043483	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4579	0.22181026043208196	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4452	0.21565828334650117	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4321	0.2093125432031068	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3164	0.15326657873053226	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2733	0.1323886092511203	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2567	0.12434744235185725	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2253	0.10913704231349215	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2152	0.10424452510369955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.844076445339199E-5
7	0.0	0.0	0.0	0.0	9.688152890678398E-5
8	0.0	0.0	0.0	0.0	9.688152890678398E-5
9	0.0	0.0	0.0	0.0	1.4532229336017597E-4
10	0.0	0.0	0.0	0.0	1.4532229336017597E-4
11	0.0	0.0	0.0	0.0	1.4532229336017597E-4
12	0.0	0.0	0.0	0.0	2.4220382226695995E-4
13	0.0	0.0	0.0	0.0	2.4220382226695995E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCCCT	40	0.005308863	14.238245	15
CCGTAAA	50	0.0014856618	13.3167715	2
GGTATCA	2065	0.0	13.270611	1
TAGGACC	975	0.0	13.169153	4
GGCGAGG	1240	0.0	12.323607	19
ATAGGAC	210	0.0	12.229688	3
AACGAAG	125	1.4297257E-9	12.149971	15
CTGCGCG	65	8.0434297E-4	11.689177	9
GACGTGA	1565	0.0	11.479786	7
ACGAAGG	125	1.8559149E-8	11.390322	16
TTAGGAC	1925	0.0	11.266135	3
TAGGACT	410	0.0	11.134894	4
TGGCGAG	2810	0.0	11.011728	18
GTGCACG	95	1.3728153E-5	10.991193	11
TGTAGGA	2755	0.0	10.944818	2
TAGGACA	710	0.0	10.85064	4
TAAAACG	140	8.463758E-9	10.848187	12
AGGACCT	1805	0.0	10.696644	5
ACCGTGC	80	3.771848E-4	10.687486	8
TAGAAAT	705	0.0	10.657778	4
>>END_MODULE