FastQCFastQC Report
Thu 26 May 2016
SRR1512834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210408
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58502.7803125356450327No Hit
GGTATCAACGCAGAGTACTTTTTTT37161.7660925440097335No Hit
TATCAACGCAGAGTACTTTTTTTTT36091.7152389642979355No Hit
GTACTGGTTCACTATCGGTCAGTCA22881.0874111250522793No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17060.8108056727881069No Hit
GGATACCACGTGTCCCGCCCTACTC14760.7014942397627466No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12170.5784000608341888No Hit
ATCATTAACTGAATCCATAGGTTAA11390.5413292270255884No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10750.5109121326185316No Hit
ATACAGGGTGACAGCCCCGTACACA10270.488099311813239No Hit
GTACATGGGAAGCAGTGGTATCAAC9990.47479183301015165No Hit
GTATTTAGCCTTGGAGGATGGTCCC8640.41063077449526636No Hit
GTACATGGGGGTTAAGCGACTAAGC8590.40825443899471503No Hit
ACTTAGATGTTTCAGTTCCCCCGGT8200.3897190220904148No Hit
GTTAATGATAGTGTGTCGAAACACA8190.3892437549903045No Hit
GGTTAATGAGGCGAACCGGGGGAAC7990.3797384129880993No Hit
GTTCACTATCGGTCAGTCAGGAGTA7830.3721341393863351No Hit
TCTAAGTACCCCGAGGAAAAGAAAT7500.3564503250826965No Hit
CCCATATTCAGACAGGATACCACGT7290.34646971598038095No Hit
CCCATGTACTCTGCGTTGATACCAC7250.34456864757993994No Hit
ACCCTGTATCGCGCGCCTTTCCAGA7110.33791490817839626No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6900.32793429907608074No Hit
GCCTTGGAGGATGGTCCCCCCATAT6780.3222310938747576No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6460.30702254667122925No Hit
CTATCGGTCAGTCAGGAGTATTTAG6440.30607201247100874No Hit
GCCCAGAGCCTGAATCAGTGTGTGT6340.30131934146990613No Hit
TATTCAGACAGGATACCACGTGTCC6260.297517204669024No Hit
AGCGTACACGGTGGATGCCCTGGCA5960.2832591916657161No Hit
GTACTTAGATGTTTCAGTTCCCCCG5950.28278392456560586No Hit
GTCAGGAGTATTTAGCCTTGGAGGA5860.2785065206646135No Hit
CATCTAAGTACCCCGAGGAAAAGAA5780.2747043838637314No Hit
GGTATCAACGCAGAGTACATGGGGG5530.2628227063609749No Hit
CGGTACTGGTTCACTATCGGTCAGT5510.2618721721607543No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5460.25949583666020304No Hit
TCACGGTACTGGTTCACTATCGGTC5430.2580700353598722No Hit
GTATCAACGCAGAGTACATGGGGGT5410.2571195011596517No Hit
GAGTACATGGGGGTTAAGCGACTAA5270.25046576175810803No Hit
TCATTAACCTATGGATTCAGTTAAT5170.24571309075700543No Hit
GTGGTATCAACGCAGAGTACTTTTT5110.24286148815634384No Hit
GGTTCACTATCGGTCAGTCAGGAGT5050.2400098855556823No Hit
GTGCTAATCTGCGATAAGCGTCGGT4950.23525721455457965No Hit
ATCAACGCAGAGTACTTTTTTTTTT4750.22575187255237444No Hit
GTTATAACCGGCGATTTCCGAATGG4650.22099920155127184No Hit
ACGCAGAGTACATGGGGGTTAAGCG4580.2176723318505No Hit
GTGGTATCAACGCAGAGTACATGGG4550.21624653055016918No Hit
GAGTACATGGGAAGCAGTGGTATCA4550.21624653055016918No Hit
GGGTACTTAGATGTTTCAGTTCCCC4480.2129196608493974No Hit
GGAGTATTTAGCCTTGGAGGATGGT4200.19961218204631004No Hit
CACACACACTGATTCAGGCTCTGGG4100.1948595110452074No Hit
GTCCCGCCCTACTCATCGAGCTCAC4050.19248317554465608No Hit
GATATGAACCGTTATAACCGGCGAT3980.18915630584388426No Hit
AGCTCACAGCATGTGCATTTTTGTG3870.18392836774267138No Hit
CACTATCATTAACTGAATCCATAGG3730.1772746283411277No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3520.1672940192388122No Hit
CCTATGGATTCAGTTAATGATAGTG3500.1663434850385917No Hit
GCCCTACTCATCGAGCTCACAGCAT3470.1649176837382609No Hit
CTATCATTAACTGAATCCATAGGTT3390.1611155469373788No Hit
GGTGATATGAACCGTTATAACCGGC3370.1601650127371583No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3350.15921447853693776No Hit
GTATCAACGCAGAGTACATGGGAAG3330.15826394433671725No Hit
GGTATCAACGCAGAGTACATGGGAA3320.15778867723660697No Hit
ATCCATAGGTTAATGAGGCGAACCG3290.1563628759362762No Hit
GTCCCCCCATATTCAGACAGGATAC3190.15161020493517358No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3190.15161020493517358No Hit
TAAGTACCCCGAGGAAAAGAAATCA3110.14780806813429148No Hit
GGGTTTCCCCATTCGGAAATCGCCG3090.14685753393407094No Hit
GGGTGACAGCCCCGTACACAAAAAT3050.1449564655336299No Hit
TTTGTGTACGGGGCTGTCACCCTGT2980.14162959583285806No Hit
TATCAACGCAGAGTACATGGGGGTT2900.13782745903197596No Hit
TTCCAGACGCTTCCACTAACACACA2890.1373521919318657No Hit
CATGTACTCTGCGTTGATACCACTG2860.13592639063153492No Hit
GGTATCAACGCAGAGTACATGGGGA2840.13497585643131438No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2810.1335500551309836No Hit
ATGATAGTGTGTCGAAACACACTGG2780.13212425383065282No Hit
CCGTTATAACCGGCGATTTCCGAAT2770.13164898673054257No Hit
GGGGTTAAGCGACTAAGCGTACACG2690.12784684992966047No Hit
GCGTACACGGTGGATGCCCTGGCAG2690.12784684992966047No Hit
TAATGATAGTGTGTCGAAACACACT2660.12642104862932968No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2620.12451998022888863No Hit
GTGGTATCCTGTCTGAATATGGGGG2610.12404471312877838No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2600.1235694460286681No Hit
AGTCAGGAGTATTTAGCCTTGGAGG2560.12166837762822705No Hit
ATTTAGCCTTGGAGGATGGTCCCCC2550.1211931105281168No Hit
GTACATGGGCATCTAAGTACCCCGA2520.119767309227786No Hit
TATCAACGCAGAGTACATGGGAAGC2480.11786624082734495No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2400.11406410402646286No Hit
GCGTTGATACCACTGCTTCCCATGT2390.1135888369263526No Hit
GGGAAACCCAGTGTGTTTCGACACA2390.1135888369263526No Hit
ACGCAGAGTACATGGGAAGCAGTGG2360.11216303562602181No Hit
CCACGTGTCCCGCCCTACTCATCGA2310.10978670012547051No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2260.10741036462491921No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2230.10598456332458842No Hit
GTGTTTCGACACACTATCATTAACT2230.10598456332458842No Hit
CATTAACCTATGGATTCAGTTAATG2210.1050340291243679No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2180.10360822782403711No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2170.10313296072392686No Hit
CCCTCACGGTACTGGTTCACTATCG2140.10170715942359607No Hit
TCGGTAAGGTGATATGAACCGTTAT2130.1012318923234858No Hit
CAACGCAGAGTACTTTTTTTTTTTT2130.1012318923234858No Hit
GGAGGATGGTCCCCCCATATTCAGA2110.10028135812326527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCCC602.5529116E-514.24679112
GCAACCG400.005266911414.246799
GGCAACC400.005266911414.246798
CTTGGTA400.005266911414.2467916
TTGGTAT400.005266911414.2467917
AAATCGC707.208151E-613.56837217
GACAACC500.001494576713.29700510
ATCGCCG751.467642E-512.66381419
GGTATCA10950.012.544021
CGAATGG1001.4236684E-712.347218519
GGGCAAC550.003058461512.0881857
GAAATCG802.8454067E-511.87232516
AATCGCC802.8454067E-511.87232518
ACAGGGT2050.011.5827563
CAGCCCA1251.7995262E-811.3974324
GCATCTA752.0619182E-411.3974329
GGAAATC855.284187E-511.17395315
CAGGGTG2050.011.1194464
TACAGGA600.005856890711.0808372
ACCCCGT600.005856890711.08083714
Produced by FastQC (version 0.11.5)