FastQCFastQC Report
Thu 26 May 2016
SRR1512834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210408
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79583.7821755826774646No Hit
TATCAACGCAGAGTACTTTTTTTTT62152.953785027185278No Hit
GGTATCAACGCAGAGTACTTTTTTT52982.517965096384168No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30481.4486141211360783No Hit
GTACTGGTTCACTATCGGTCAGTCA19830.9424546595186495No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17670.8397969658948328No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13620.6473137903501768No Hit
GGATACCACGTGTCCCGCCCTACTC12780.6073913539409148No Hit
ATCATTAACTGAATCCATAGGTTAA11840.5627162465305502No Hit
ACTTAGATGTTTCAGTTCCCCCGGT9620.45720695030607206No Hit
CCCATATTCAGACAGGATACCACGT9610.45673168320596175No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9600.45625641610585144No Hit
GTACATGGGAAGCAGTGGTATCAAC9370.4453252728033155No Hit
CTTATACACATCTCCGAGCCCACGA9230.43867153340177184No Hit
CCCATGTACTCTGCGTTGATACCAC9110.43296832820044867No Hit
TCTAAGTACCCCGAGGAAAAGAAAT9060.43059199269989734No Hit
ATACAGGGTGACAGCCCCGTACACA9050.43011672559978714No Hit
ACCCTGTATCGCGCGCCTTTCCAGA8460.4020759666932816No Hit
GTATTTAGCCTTGGAGGATGGTCCC8300.3944716930915174No Hit
GTTAATGATAGTGTGTCGAAACACA8290.39399642599140716No Hit
ATCAACGCAGAGTACTTTTTTTTTT8220.3906695562906353No Hit
GTTCACTATCGGTCAGTCAGGAGTA7680.36500513288468117No Hit
TATTCAGACAGGATACCACGTGTCC7620.3621535302840196No Hit
CTATCGGTCAGTCAGGAGTATTTAG7180.3412417778791681No Hit
GCCTTGGAGGATGGTCCCCCCATAT6980.33173643587696283No Hit
GTGGTATCAACGCAGAGTACTTTTT6960.3307859016767423No Hit
TCACGGTACTGGTTCACTATCGGTC6830.32460742937530895No Hit
GTACATGGGGGTTAAGCGACTAAGC6620.31462682027299343No Hit
GTCAGGAGTATTTAGCCTTGGAGGA6520.30987414927189083No Hit
CATCTAAGTACCCCGAGGAAAAGAA6420.3051214782707882No Hit
GCCCAGAGCCTGAATCAGTGTGTGT6360.30226987567012664No Hit
TCATTAACCTATGGATTCAGTTAAT6260.297517204669024No Hit
GGTTAATGAGGCGAACCGGGGGAAC6040.2870613284665982No Hit
ATACACATCTCCGAGCCCACGAGAC5420.25759476825976196No Hit
AGCGTACACGGTGGATGCCCTGGCA5240.24903996045777727No Hit
CGGTACTGGTTCACTATCGGTCAGT5170.24571309075700543No Hit
GTACTTAGATGTTTCAGTTCCCCCG5140.24428728945667466No Hit
GGTATCAACGCAGAGTACATGGGGG5120.2433367552564541No Hit
GTATCAACGCAGAGTACATGGGGGT5100.2423862210562336No Hit
GTGGATGCCCTGGCAGTCAGAGGCG4960.2357324816546899No Hit
ACGCAGAGTACATGGGGGTTAAGCG4620.21957340025094102No Hit
GGTTCACTATCGGTCAGTCAGGAGT4610.21909813315083077No Hit
CACACACACTGATTCAGGCTCTGGG4560.2167217976502795No Hit
CCTATGGATTCAGTTAATGATAGTG4260.2024637846469716No Hit
GAGTACATGGGAAGCAGTGGTATCA4160.197711113645869No Hit
GTGGTATCAACGCAGAGTACATGGG4040.19200790844454582No Hit
GAGTACATGGGGGTTAAGCGACTAA3970.18868103874377398No Hit
CACTATCATTAACTGAATCCATAGG3960.18820577164366373No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3920.18630470324322268No Hit
GCCCTACTCATCGAGCTCACAGCAT3900.18535416904300217No Hit
GTTATAACCGGCGATTTCCGAATGG3840.18250256644234059No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG3770.17917569674156875No Hit
GTCCCGCCCTACTCATCGAGCTCAC3730.1772746283411277No Hit
ACACATCTCCGAGCCCACGAGACAA3650.1734724915402456No Hit
GGAGTATTTAGCCTTGGAGGATGGT3620.17204669023991484No Hit
AGCTCACAGCATGTGCATTTTTGTG3490.16586821793848142No Hit
GATATGAACCGTTATAACCGGCGAT3440.16349188243793011No Hit
TATCAACGCAGAGTACATGGGGGTT3390.1611155469373788No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3370.1601650127371583No Hit
GGGTACTTAGATGTTTCAGTTCCCC3340.1587392114368275No Hit
CTATCATTAACTGAATCCATAGGTT3280.15588760883616593No Hit
CAACGCAGAGTACTTTTTTTTTTTT3240.15398654043572488No Hit
GTGCTAATCTGCGATAAGCGTCGGT3110.14780806813429148No Hit
TTCCAGACGCTTCCACTAACACACA3110.14780806813429148No Hit
TCAACGCAGAGTACTTTTTTTTTTT2970.1411543287327478No Hit
ATCCATAGGTTAATGAGGCGAACCG2960.14067906163263755No Hit
TAAGTACCCCGAGGAAAAGAAATCA2940.139728527432417No Hit
TAATGATAGTGTGTCGAAACACACT2920.13877799323219647No Hit
ACGCAGAGTACATGGGAAGCAGTGG2910.13830272613208622No Hit
GTCCCCCCATATTCAGACAGGATAC2910.13830272613208622No Hit
GGGTTTCCCCATTCGGAAATCGCCG2870.13640165773164517No Hit
ATGATAGTGTGTCGAAACACACTGG2870.13640165773164517No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2850.13545112353142466No Hit
CATTAACCTATGGATTCAGTTAATG2830.13450058933120412No Hit
GTATCAACGCAGAGTACATGGGAAG2830.13450058933120412No Hit
GGTATCAACGCAGAGTACATGGGAA2800.13307478803087333No Hit
CATGTACTCTGCGTTGATACCACTG2780.13212425383065282No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2780.13212425383065282No Hit
TTTGTGTACGGGGCTGTCACCCTGT2770.13164898673054257No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2670.12689631572943993No Hit
AACGCAGAGTACTTTTTTTTTTTTT2670.12689631572943993No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2650.12594578152921942No Hit
TATCAACGCAGAGTACATGGGAAGC2650.12594578152921942No Hit
AGTCAGGAGTATTTAGCCTTGGAGG2640.12547051442910914No Hit
CCGTTATAACCGGCGATTTCCGAAT2600.1235694460286681No Hit
ATTTAGCCTTGGAGGATGGTCCCCC2600.1235694460286681No Hit
GGTATCAACGCAGAGTACATGGGGA2520.119767309227786No Hit
TCGGTAAGGTGATATGAACCGTTAT2500.11881677502756549No Hit
CCACGTGTCCCGCCCTACTCATCGA2400.11406410402646286No Hit
CCCTCACGGTACTGGTTCACTATCG2390.1135888369263526No Hit
CTACTGGGGGAATCTCGGTTGATTT2350.11168776852591156No Hit
GGTGATATGAACCGTTATAACCGGC2330.11073723432569105No Hit
GCGTTGATACCACTGCTTCCCATGT2310.10978670012547051No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2270.10788563172502946No Hit
ATTCAGACAGGATACCACGTGTCCC2230.10598456332458842No Hit
CATTAACTGAATCCATAGGTTAATG2220.10550929622447816No Hit
AACACACACACTGATTCAGGCTCTG2210.1050340291243679No Hit
GTATCGCGCGCCTTTCCAGACGCTT2200.10455876202425764No Hit
GTACATGGGCATCTAAGTACCCCGA2200.10455876202425764No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2190.10408349492414737No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2170.10313296072392686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA359.7310076E-519.039561
ACCCCGT555.8977093E-717.25933314
GACAACC402.7582026E-416.61605610
AGACAAC402.7582026E-416.6160569
CGAGACG350.002173055716.27308513
CAACCCC601.4639445E-615.82105612
CTTATAC1003.8198777E-1115.2316491
TTATACA1058.367351E-1114.4994222
CGCAGAT400.005286191614.2389517
GGTATCA10900.014.1486651
ACAACCC707.2492985E-613.560904511
GTACACA2750.013.46227919
CCGTACA2800.013.22188217
GGTGACA1600.013.0616837
CCCCGTA2850.012.98991915
CCCGTAC2850.012.98991916
ACAGGGA604.0112526E-412.6869953
ACAGGGT1650.012.6869943
GGGTGAC1800.012.1525796
TACAGGG2350.012.1471212
Produced by FastQC (version 0.11.5)