FastQCFastQC Report
Thu 26 May 2016
SRR1512833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508271
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78580.31328353276021614No Hit
CTGTAGGACGTGGAATATGGCAAGA68310.27233899367333114No Hit
GTCCTAAAGTGTGTATTTCTCATTT68020.27118281876240646No Hit
GTATCAACGCAGAGTACTTTTTTTT62160.24782011194165224No Hit
CTTTAGGACGTGAAATATGGCGAGG55430.22098888038812395No Hit
GTCCTACAGTGTGCATTTCTCATTT42350.16884140509538242No Hit
TATCAACGCAGAGTACTTTTTTTTT35880.1430467441516487No Hit
GGTATCAACGCAGAGTACTTTTTTT33690.13431563016914838No Hit
CTGAAGGACCTGGAATATGGCGAGA28630.11414237137853127No Hit
CTGTAGGACCTGGAATATGGCGAGA27260.10868044162692149No Hit
GTCCTTCAGTGTGCATTTCTCATTT25650.1022616774662706No Hit
ATTTAGAAATGTCCACTGTAGGACG25520.10174339216137332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.013.372841
TAGGACC11900.013.1706784
TACGACT550.003072124212.0894494
AGGACCT23300.011.2924025
GTCCTAA18250.011.1129411
ACCGCTC600.005881774211.0822158
ATTTAGA9550.011.0670491
GGACCTG23000.011.0271496
ACGCCGC700.0014932110.8560488
TACGACC700.001493458510.8558324
GTCCTAC35350.010.7202741
TGTAGGA37400.010.7179572
TAGACAG3400.010.6161535
AATGTCC9450.010.5544918
GTCTAAT1451.3942554E-810.5066491
GTAGGAC36950.010.48858453
AGGACGT36150.010.4577265
TAGAAAT10750.010.4266474
CACCTTT8400.010.40350514
CTGTAGG36750.010.389611
Produced by FastQC (version 0.11.5)