FastQCFastQC Report
Thu 26 May 2016
SRR1512833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508271
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77960.31081171053685985No Hit
GTCCTACAGTGGACATTTCTAAATT72480.2889639915304208No Hit
CTGTAGGACGTGGAATATGGCAAGA65530.26125566176860476No Hit
GTCCTAAAGTGTGTATTTCTCATTT61610.245627366420933No Hit
CTTTAGGACGTGAAATATGGCGAGG60670.2418797649855219No Hit
TATCAACGCAGAGTACTTTTTTTTT54740.2182379814621307No Hit
GGTATCAACGCAGAGTACTTTTTTT44300.17661568466884162No Hit
GTCCTACAGTGTGCATTTCTCATTT38440.1532529778480874No Hit
CTGAAGGACCTGGAATATGGCGAGA29840.11896641152411364No Hit
CTGTAGGACCTGGAATATGGCGAGA27930.1113516043521613No Hit
ATTTAGAAATGTCCACTGTAGGACG26200.10445442298698984No Hit
TTTCTAAATTTTCCACCTTTTTCAG25790.10281983087154459No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25450.10146431545873631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT250.005989085419.0240961
ATCGGAC500.001486184513.3163353
AAGACCG655.3997057E-513.16815955
TGGTCGG655.4709635E-513.14977310
TCGCGCG1750.013.0263569
TAGGACC10350.012.9561124
TCCAACG1550.012.85928518
GGTATCA19650.012.8279521
GGCGAGG15250.012.26025919
ATCGCGC1950.012.1803318
TATCGCG2050.011.5938047
CCAACGA1553.1832315E-1011.02158519
ATCCCGT1408.400093E-910.85378110
CGCGCCT2300.010.73088112
GTATCAA42550.010.7303941
ACCTTTT9350.010.66046715
AGATCGT909.4151044E-510.5670416
AAATGTC9000.010.5632447
ACCGCCC909.512807E-510.5562878
GCGCCAC1803.6379788E-1110.54744713