FastQCFastQC Report
Thu 26 May 2016
SRR1512832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423519
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106450.7477947256060509No Hit
TATCAACGCAGAGTACTTTTTTTTT63590.44670987882845264No Hit
GGTATCAACGCAGAGTACTTTTTTT63210.4440404378164254No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29360.2062494424029465No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23910.16796403841466112No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20560.1444308084402105No Hit
GTACTGGTTCACTATCGGTCAGTCA19360.1360009947180192No Hit
GTCCTACAGTGGACATTTCTAAATT17800.12504223687917057No Hit
GTCCTAAAGTGTGTATTTCTCATTT17450.1225835412101981No Hit
CTGTAGGACGTGGAATATGGCAAGA14760.10368670878295268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.013.4422161
CCATCGC1153.783498E-1013.21504319
GCGTGCG1153.783498E-1013.2150439
TAGGACC3750.013.1709934
GGCGAGG5350.012.42769819
ACTGCGT550.0030723112.0887618
GTCTAGA1302.5047484E-911.7240371
GGCGTGC1604.5474735E-1111.2792468
ACCATCG1354.7330104E-911.25805118
GACTTAA600.00575789911.113411
AAGGCGT2150.011.0445496
TTAGGAC7800.010.9595913
CGTGCGC1303.272544E-810.95959110
ACGAAGG700.001492603210.85597816
CCCCGTA1752.0008883E-1110.85597715
TGGCGAG10250.010.84273918
AGGCGTG1951.8189894E-1210.7160447
CTACACT1951.8189894E-1210.7160444
TATACTG1701.382432E-1010.6157615
GTATCAA41400.010.5842011
Produced by FastQC (version 0.11.5)