##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512832_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1423519
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.74483375353613	31.0	30.0	34.0	22.0	34.0
2	28.866925555612536	31.0	30.0	34.0	18.0	34.0
3	29.01827794360314	31.0	30.0	34.0	22.0	34.0
4	32.40009792633607	35.0	33.0	37.0	22.0	37.0
5	32.61404730108976	35.0	33.0	37.0	25.0	37.0
6	32.473646646093236	36.0	33.0	37.0	23.0	37.0
7	32.391736956092615	37.0	33.0	37.0	22.0	37.0
8	32.246180065036015	36.0	33.0	37.0	20.0	37.0
9	33.47338180944546	38.0	34.0	39.0	18.0	39.0
10	33.36788831058806	38.0	33.0	39.0	17.0	39.0
11	33.38029980632503	38.0	34.0	39.0	17.0	39.0
12	33.285522708161956	38.0	33.0	39.0	17.0	39.0
13	33.222748695310706	38.0	33.0	39.0	17.0	39.0
14	34.07088489862095	39.0	33.0	41.0	16.0	41.0
15	34.23389782644278	39.0	34.0	41.0	17.0	41.0
16	34.180257516759525	39.0	34.0	41.0	17.0	41.0
17	33.97688896319614	39.0	33.0	41.0	11.0	41.0
18	33.98412736324559	39.0	33.0	41.0	11.0	41.0
19	34.12206721511971	39.0	34.0	41.0	11.0	41.0
20	34.09601628078023	39.0	34.0	41.0	10.0	41.0
21	34.004566851583995	39.0	34.0	41.0	10.0	41.0
22	34.01840509329345	39.0	34.0	41.0	8.0	41.0
23	33.701567032122504	39.0	33.0	41.0	2.0	41.0
24	33.76151354495444	39.0	34.0	41.0	2.0	41.0
25	33.49847385247404	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7005434565339179
1101	2	0.3228777405649019
1101	3	0.7447145889477511
1101	4	0.8244316303719827
1101	5	0.7009820552932453
1101	6	0.7134179020457694
1101	7	0.4429902564752055
1101	8	0.7504437388373759
1101	9	0.9211340800904537
1101	10	0.8822586581336012
1101	11	0.9559772707675478
1101	12	0.7914209596695017
1101	13	1.281032953299011
1101	14	1.3156973362992233
1101	15	1.0792008638411232
1101	16	0.9721061513594833
1101	17	0.5599291501123673
1101	18	0.7915601119086233
1101	19	0.3384716278062996
1101	20	-0.08110957991195988
1101	21	0.8340452756662771
1101	22	0.9268973961123805
1101	23	0.3596514000448039
1101	24	0.9133283135199264
1101	25	0.7574700992259764
1102	1	1.1583518914236883
1102	2	1.2472076392366809
1102	3	1.137583101887639
1102	4	1.3259994792012364
1102	5	1.3599449814879634
1102	6	1.1665732833958984
1102	7	1.3400727910500407
1102	8	1.1718869813404567
1102	9	1.6351822452280658
1102	10	1.5876006408804173
1102	11	1.366123785898317
1102	12	1.5444744993107449
1102	13	1.3821925794800336
1102	14	1.5787895586735914
1102	15	1.5042145568439977
1102	16	1.53088507782806
1102	17	1.7690991829755731
1102	18	1.6544922973118759
1102	19	1.614162268638836
1102	20	1.7714647046286345
1102	21	1.8717797683405166
1102	22	1.8222238030684252
1102	23	1.8422587189548452
1102	24	1.0051963814372158
1102	25	1.4026236320207204
1103	1	0.789458103318033
1103	2	0.8737105636003122
1103	3	0.9784530483639244
1103	4	1.1285209672762804
1103	5	0.8847048693115198
1103	6	0.5161559307929267
1103	7	0.5001157258371407
1103	8	0.7633335279441411
1103	9	0.988565324454747
1103	10	1.0643517432041918
1103	11	0.9594192583050614
1103	12	0.938958744146575
1103	13	1.1232966404987224
1103	14	1.0823294036450903
1103	15	1.0352574833585066
1103	16	1.0920617722630865
1103	17	0.8137778338801809
1103	18	1.035902113577464
1103	19	1.0941876655167562
1103	20	1.2427467919707311
1103	21	1.0720891635308334
1103	22	1.2040997802691393
1103	23	0.5152747429784696
1103	24	0.6605499936674306
1103	25	0.9457048441218774
1104	1	1.0873789684433177
1104	2	1.101943846802392
1104	3	0.9690540471473028
1104	4	1.23610526042156
1104	5	0.9923118879033765
1104	6	0.8726905923745036
1104	7	1.0426991546038238
1104	8	0.8565569621000009
1104	9	1.0634326849509392
1104	10	1.196419458541996
1104	11	0.9458176727181922
1104	12	1.1541975358345766
1104	13	1.2613707725287
1104	14	1.3391021954418534
1104	15	1.098769425174872
1104	16	1.018745423495652
1104	17	1.435332595307635
1104	18	1.2258909050625633
1104	19	0.9593141691499838
1104	20	1.015871760013077
1104	21	1.1913356688847045
1104	22	0.9715681411719572
1104	23	1.4344407353216155
1104	24	1.215724131459929
1104	25	0.5548486710383784
1105	1	0.7223868791722801
1105	2	0.7238477408297719
1105	3	0.8977249089096091
1105	4	0.9982950108961646
1105	5	0.7143078674480918
1105	6	0.8613112823050812
1105	7	0.7869761555071122
1105	8	0.9435829672737199
1105	9	0.6199750295137036
1105	10	0.7471779061702577
1105	11	0.91505168573795
1105	12	0.9879140929383823
1105	13	0.9020040461488747
1105	14	1.1013809965251582
1105	15	1.0313141880806214
1105	16	0.9331530133229577
1105	17	1.3527539292777604
1105	18	1.119485011589454
1105	19	0.8177013388341265
1105	20	1.1930365483641339
1105	21	1.0266994625843893
1105	22	0.9861904108523447
1105	23	1.2510695588073375
1105	24	1.2228270156613874
1105	25	1.5200873865692088
1106	1	0.6467576335825598
1106	2	0.7408622841006682
1106	3	0.5770966695913629
1106	4	0.7687767093846389
1106	5	0.7010373058997175
1106	6	0.7589473998734348
1106	7	0.6359266591724619
1106	8	0.6974819636910681
1106	9	0.8772004253613446
1106	10	0.6210346473336088
1106	11	0.4163647274236624
1106	12	0.5644840190283489
1106	13	0.6802262944445232
1106	14	0.9870624460123665
1106	15	0.6903376729087327
1106	16	0.8427914644633887
1106	17	0.8427278802906741
1106	18	0.8501578772143219
1106	19	0.873751067385264
1106	20	0.9719310023009413
1106	21	1.007049058405471
1106	22	0.9083481828124889
1106	23	1.1875114969432303
1106	24	0.8561563565006125
1106	25	1.108617684303347
1107	1	0.9196219198511351
1107	2	0.8822113541869321
1107	3	0.8629416753467396
1107	4	1.002071161589143
1107	5	0.8170938379293631
1107	6	0.906107622457796
1107	7	0.5755449127711927
1107	8	0.741732891664789
1107	9	0.7345143617805974
1107	10	1.0965415570194494
1107	11	0.9534743802903591
1107	12	0.9865018930308622
1107	13	0.7587762789244081
1107	14	1.0554427115357186
1107	15	0.7245918665739381
1107	16	0.7389497502663573
1107	17	1.056637947673856
1107	18	0.8858553610152029
1107	19	0.794759729551096
1107	20	0.9935961169558141
1107	21	0.9666919016910782
1107	22	0.8241475012206365
1107	23	1.0472827853716922
1107	24	0.9228997345836163
1107	25	1.0412098511704784
1108	1	0.8539857474903592
1108	2	0.7912318681078112
1108	3	0.8385222521878859
1108	4	0.9546164144742733
1108	5	0.7839240828799916
1108	6	0.9100468926691576
1108	7	0.7766483715334118
1108	8	0.8125417319930364
1108	9	0.9254770217826973
1108	10	0.8739342946376212
1108	11	0.6116516607640818
1108	12	0.839157943317673
1108	13	0.7914734759160922
1108	14	0.8122281358307148
1108	15	0.8135107370965002
1108	16	0.48078794515246415
1108	17	0.8765753824535096
1108	18	0.904136546373735
1108	19	0.8825040549952092
1108	20	0.884753781928552
1108	21	0.9026285430208674
1108	22	0.8096265195656969
1108	23	1.1953898902984932
1108	24	0.9669514462124411
1108	25	1.1722072284636127
1109	1	0.5342822301980661
1109	2	0.48760241265897974
1109	3	0.6107710759930427
1109	4	0.676660744541401
1109	5	0.25932144600204055
1109	6	0.523108491307255
1109	7	0.4305350810536126
1109	8	0.36085596342870474
1109	9	0.5388559415623178
1109	10	0.4918204272102926
1109	11	0.6082839193428526
1109	12	0.42640189912805226
1109	13	0.47845533812811425
1109	14	0.6172342943660283
1109	15	0.449474495681919
1109	16	0.07521964234611289
1109	17	0.46277248215346134
1109	18	0.5733770822517101
1109	19	0.45659246840848056
1109	20	0.47275744858193036
1109	21	0.41893414301782883
1109	22	0.2907829197926759
1109	23	0.5901220177355739
1109	24	0.5829437105606914
1109	25	0.1222868434270481
1110	1	0.62394021619113
1110	2	0.6071248622861383
1110	3	0.5461385880621243
1110	4	0.5639343784118935
1110	5	0.522969142110469
1110	6	0.5842494445948034
1110	7	0.5727496727446422
1110	8	0.6859494563232431
1110	9	0.66813451639063
1110	10	0.6827669152771989
1110	11	0.5363737178515038
1110	12	0.5791511568511325
1110	13	0.5516619744237587
1110	14	0.4835768523316233
1110	15	0.4719003246522533
1110	16	0.5410953024626082
1110	17	0.7357898661656108
1110	18	-0.024364356065646575
1110	19	0.6030250062022802
1110	20	0.612008598827849
1110	21	0.6115159068037812
1110	22	0.7823379616094996
1110	23	0.9745489346232219
1110	24	0.8883175688141307
1110	25	1.060266490307221
1111	1	0.4578028379637509
1111	2	0.40543942611962436
1111	3	0.5635441963810415
1111	4	0.35975773507821174
1111	5	0.3732045723578139
1111	6	0.37159807612069073
1111	7	0.2600244101053022
1111	8	0.3871350089637673
1111	9	0.4447801413596011
1111	10	0.27271597221962196
1111	11	0.03141898996151582
1111	12	-0.21875899395406861
1111	13	0.26286429168367675
1111	14	0.06633297103581981
1111	15	0.2192046196790045
1111	16	0.4363836424597807
1111	17	-5.171822302969531E-4
1111	18	0.2611090439442876
1111	19	0.19417049942196485
1111	20	0.500646845836755
1111	21	0.36024061189541356
1111	22	0.04852918519758731
1111	23	0.35629421169770126
1111	24	0.3137222682539118
1111	25	0.46043986473105747
1112	1	0.3782893881869853
1112	2	0.2536953875665411
1112	3	0.18837604447946887
1112	4	0.40538217508653673
1112	5	0.30054698844338645
1112	6	0.19773447322807414
1112	7	-0.07253601198708282
1112	8	0.15674538302632612
1112	9	0.32380179611303106
1112	10	0.15690812573264168
1112	11	0.03628297196196684
1112	12	0.16791560325573585
1112	13	0.10745440390529382
1112	14	0.06427438699882515
1112	15	-0.1443451598398653
1112	16	0.11971496465298515
1112	17	0.38618857973369103
1112	18	0.16065059900851253
1112	19	0.2667281280882179
1112	20	-0.06889967848552914
1112	21	0.11432618764999347
1112	22	-0.006739416027528478
1112	23	0.16137558686148168
1112	24	0.21859812706617276
1112	25	0.07792310179887352
1113	1	-0.17335402588539495
1113	2	-0.05920461054556014
1113	3	-0.028628241046025238
1113	4	0.0076117810824953835
1113	5	-0.13129994089263874
1113	6	-0.541084458599979
1113	7	-0.5399981124609461
1113	8	-0.5311286565062261
1113	9	-0.549945112456129
1113	10	-0.5021566102450024
1113	11	-0.493111579568172
1113	12	-0.5404798733437843
1113	13	-0.41121689440126374
1113	14	-0.2987964657641058
1113	15	-0.4717914019814913
1113	16	-0.3791806157808537
1113	17	-0.37306683381007844
1113	18	-0.4461760354380502
1113	19	-0.7634876585039407
1113	20	-0.5815863442711802
1113	21	-0.2918453466436475
1113	22	-0.7307572377723233
1113	23	-4.225860343588405
1113	24	-1.9745997811442884
1113	25	-1.0491229062435679
1114	1	-1.0405701901089017
1114	2	-1.2104692126853749
1114	3	-1.0578895563039517
1114	4	-0.9644647965841422
1114	5	-1.0900554397384816
1114	6	-1.8085457130742206
1114	7	-1.6827142958321275
1114	8	-1.5642260252896492
1114	9	-1.9483275819848451
1114	10	-1.414318888019931
1114	11	-1.5840944093991105
1114	12	-2.4134774134785353
1114	13	-1.6599940912304056
1114	14	-3.7186308505900243
1114	15	-2.364299609001275
1114	16	-2.3762167999994226
1114	17	-2.1223777211785624
1114	18	-2.1530560805450882
1114	19	-2.1239532485008716
1114	20	-1.727334378782622
1114	21	-1.8744936413190487
1114	22	-2.0838464912003687
1114	23	-6.324885197271644
1114	24	-3.808673762874964
1114	25	-3.5718230870465177
1115	1	-1.4783105068646378
1115	2	-1.3369585681740865
1115	3	-1.0880984878941895
1115	4	-0.8822557806295421
1115	5	-1.058677575000516
1115	6	-1.3471257797220666
1115	7	-1.7551252833169997
1115	8	-2.1617378444124533
1115	9	-3.0966441884607825
1115	10	-3.595000584522378
1115	11	-2.5861025246018947
1115	12	-2.8306981268765696
1115	13	-2.007353405542819
1115	14	-2.8135119617335818
1115	15	-2.5407492970153704
1115	16	-2.5795162577961506
1115	17	-2.432428518259556
1115	18	-5.10421114224464
1115	19	-3.1429688948454597
1115	20	-3.333594944607917
1115	21	-3.0199576777644523
1115	22	-2.5471727346824125
1115	23	-3.210454672947126
1115	24	-2.8589443777938257
1115	25	-2.953167854995847
1116	1	-1.2841191257832207
1116	2	-1.0802805487803049
1116	3	-1.7054319208789153
1116	4	-1.3411882534846775
1116	5	-1.64419541752466
1116	6	-3.9994249025001167
1116	7	-2.292649899239091
1116	8	-2.484865817659532
1116	9	-3.7428323488323194
1116	10	-3.125770704399528
1116	11	-2.607943778193256
1116	12	-2.4136248883163454
1116	13	-3.4923124905344487
1116	14	-6.885278998211206
1116	15	-4.736244474641083
1116	16	-3.6132811338083783
1116	17	-4.526423319585383
1116	18	-3.2937452690811675
1116	19	-3.018029728545244
1116	20	-3.8007210650343097
1116	21	-4.436723054351123
1116	22	-4.024776156317309
1116	23	-3.022177848327207
1116	24	-4.116187933332387
1116	25	-3.8725136667694287
1201	1	0.7456097915256272
1201	2	0.7350771601337271
1201	3	1.0068449095668761
1201	4	1.0823365502172066
1201	5	0.6525333267139217
1201	6	1.0151419208406836
1201	7	1.1357659612844913
1201	8	1.0151549052276465
1201	9	1.3037729689793451
1201	10	1.037226872616742
1201	11	1.295405131464065
1201	12	1.0869778972040507
1201	13	1.2623658720386501
1201	14	1.4233189504506853
1201	15	1.5309559771634014
1201	16	1.4777929949194615
1201	17	1.7082811401621143
1201	18	1.4547683761430008
1201	19	1.4451085819245932
1201	20	1.5428332061576882
1201	21	1.6093562209399082
1201	22	1.3823112988210582
1201	23	1.7990205266340809
1201	24	1.6745983522153338
1201	25	1.6001006962409008
1202	1	0.8609852895838301
1202	2	0.9357832679664781
1202	3	0.8915566242320629
1202	4	0.995658451328481
1202	5	0.8861186312649565
1202	6	1.102254402137234
1202	7	1.1218468106313395
1202	8	1.0708531534077395
1202	9	1.3781103624327145
1202	10	1.21950188480551
1202	11	1.227955441647147
1202	12	1.3873349439921014
1202	13	1.3795369014429966
1202	14	1.6732012658118975
1202	15	1.4579931823213528
1202	16	1.1326466456650834
1202	17	1.5250329180407647
1202	18	1.411943146237654
1202	19	1.464402558152301
1202	20	1.3236302253739112
1202	21	1.4670635140250923
1202	22	1.3723112567908302
1202	23	1.4350656502870152
1202	24	1.5322075047178174
1202	25	1.839739824702093
1203	1	1.1283766958901644
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2211	18	-0.44182733866190205
2211	19	-0.2903500033332733
2211	20	-0.366395904755187
2211	21	-0.3066822863452572
2211	22	-0.2388582092240057
2211	23	-0.28699064064381474
2211	24	-0.5599756062212435
2211	25	-0.0734274036698892
2212	1	-0.2386948967487328
2212	2	-0.06883799498571008
2212	3	-0.35799096779130224
2212	4	-0.058225778391509664
2212	5	-0.1796944295883236
2212	6	-0.38423652763454896
2212	7	-0.31356747116669226
2212	8	-0.2724136512860511
2212	9	-0.21833402874094787
2212	10	-0.7056184704962192
2212	11	-0.7780188947030453
2212	12	-0.3185954169665024
2212	13	-0.15689768232748946
2212	14	-0.09996548474276068
2212	15	-0.4372040651268847
2212	16	-0.5807542677758804
2212	17	-0.30193667131572965
2212	18	-0.12068550101693631
2212	19	-0.44333840654469725
2212	20	-0.23570901804689015
2212	21	0.05415625067526264
2212	22	0.2209990610703727
2212	23	0.14833496149713454
2212	24	0.06268273454592332
2212	25	0.32889195404643345
2213	1	-0.49889323522696216
2213	2	-0.34533682151048595
2213	3	-0.6284566265914258
2213	4	-0.3302846826149235
2213	5	-0.3439899760264282
2213	6	-0.3334968541381613
2213	7	-0.4915382761691305
2213	8	-0.2902403626236847
2213	9	-0.7279762011046245
2213	10	-0.44505523374269274
2213	11	-0.5167360518985618
2213	12	-0.5872808738693109
2213	13	-0.6097391855672853
2213	14	-0.5602617304059194
2213	15	-0.4641751821153832
2213	16	-0.41197137401889705
2213	17	-0.5324994690938851
2213	18	-0.48200259957788205
2213	19	-0.4479868421462214
2213	20	-0.28853029706434086
2213	21	-0.5190781668749693
2213	22	-0.6666984974426953
2213	23	-0.48028364572808613
2213	24	-0.3637665504729455
2213	25	-0.10460053398757196
2214	1	-2.6931166690601316
2214	2	-1.429269827328838
2214	3	-1.481499802207484
2214	4	-3.4621045103042576
2214	5	-1.765018870298075
2214	6	-1.463101780832666
2214	7	-1.310527374079193
2214	8	-1.0420720967857875
2214	9	-0.9013733433905884
2214	10	-1.0365138850436182
2214	11	-1.0685091368471902
2214	12	-0.9448417650804402
2214	13	-1.311744579207481
2214	14	-1.2382422224170213
2214	15	-1.6397747810045402
2214	16	-1.3923650342837917
2214	17	-3.4730821155101843
2214	18	-3.0692255412554346
2214	19	-1.9679436320070138
2214	20	-1.7704586318200768
2214	21	-1.7309037912422127
2214	22	-2.7392677665394167
2214	23	-1.8335395802978844
2214	24	-2.9880693634674564
2214	25	-2.865283040625247
2215	1	-2.3131715704815434
2215	2	-3.897808144525772
2215	3	-2.3968139912694433
2215	4	-3.952042183355079
2215	5	-2.610205621912126
2215	6	-2.570416621572697
2215	7	-2.629287802156366
2215	8	-2.149077472311184
2215	9	-4.4244080346048875
2215	10	-2.649989375889831
2215	11	-2.339093076421239
2215	12	-2.2647096774673976
2215	13	-2.4457882498251493
2215	14	-2.073395505941189
2215	15	-2.2855613466119884
2215	16	-2.2422488210900937
2215	17	-2.651799504999172
2215	18	-2.3885858651951075
2215	19	-2.5462295303812574
2215	20	-2.2796130304014497
2215	21	-2.7689143883790877
2215	22	-2.748139716710604
2215	23	-2.7674967971413054
2215	24	-2.696936892707008
2215	25	-3.4544691962322176
2216	1	-3.084482801066965
2216	2	-3.1681635806070148
2216	3	-4.595932868210902
2216	4	-5.214422709042054
2216	5	-4.709503710323116
2216	6	-3.5080062273074617
2216	7	-5.162382186863656
2216	8	-3.142715465142725
2216	9	-3.9878936976873227
2216	10	-2.86879164590178
2216	11	-3.061539312980379
2216	12	-3.364873954647802
2216	13	-2.801985979813203
2216	14	-3.927144012512276
2216	15	-3.3560810598736346
2216	16	-3.8217440421175723
2216	17	-3.3764410820601043
2216	18	-2.7831945868199597
2216	19	-3.5451691958531804
2216	20	-4.3289260531015685
2216	21	-3.9957363716526864
2216	22	-3.5977812937715363
2216	23	-3.564405399291843
2216	24	-4.174012758895778
2216	25	-4.2100844889442826
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75027.0
3	3.0
4	663.0
5	4124.0
6	5835.0
7	5557.0
8	5122.0
9	4458.0
10	3756.0
11	3590.0
12	3198.0
13	3137.0
14	2940.0
15	2931.0
16	2942.0
17	3286.0
18	3603.0
19	4317.0
20	5787.0
21	7483.0
22	9521.0
23	11518.0
24	13112.0
25	14605.0
26	16139.0
27	17463.0
28	19193.0
29	22722.0
30	27402.0
31	33553.0
32	41933.0
33	52902.0
34	68402.0
35	89797.0
36	121696.0
37	188176.0
38	527626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01232425513499	18.637995357875646	12.755833140087697	23.593847246901667
2	15.562189250463513	20.571206563564154	40.608118883806284	23.25848530216605
3	19.19917886115461	26.12216337206484	29.05333678987262	25.62532097690793
4	12.409790917266188	16.5572307778777	37.94514388489208	33.08783441996403
5	13.071675927766933	38.867815563004406	34.331433806610804	13.729074702617863
6	31.151716695199163	36.47668830917738	18.33578818227517	14.035806813348284
7	27.99540766144369	32.54380544007919	21.599528673642354	17.861258224834764
8	26.091059643398367	35.00813974339591	20.819248471380803	18.08155214182492
9	28.039133659798708	14.531552655724333	20.053785931990678	37.375527752486285
10	16.739640514442776	28.492144910803624	32.612665316175864	22.155549258577736
11	33.91576358455106	23.215532473339948	23.969192671658256	18.89951127045074
12	24.018878817092475	25.557564289446134	30.28633950790473	20.137217385556664
13	29.28918096317488	22.32491069149845	25.626354743703406	22.759553601623267
14	22.07088089604815	22.605106715087768	27.562550756023047	27.76146163284104
15	23.924431991956663	30.231210824758577	23.872122168748398	21.972235014536366
16	22.27118141172616	28.1018482386996	27.245050704049085	22.381919645525155
17	21.479230773558054	28.538770722816338	27.279369160061428	22.702629343564183
18	21.4520080558509	26.76995257624086	29.835409177594386	21.942630190313853
19	23.19765594016938	26.705378061917607	28.618610721247016	21.478355276666
20	24.07384750389193	26.147503751299066	28.689738386442166	21.08891035836684
21	23.715213550465883	26.125789798201154	28.03691337688077	22.122083274452194
22	23.842979187513492	26.964043488441924	27.670614642365155	21.52236268167943
23	22.657039954885335	26.976192451731666	28.53368903789604	21.83307855548696
24	23.099488190554272	26.204938936541676	28.788666082129982	21.90690679077407
25	22.643815148798538	26.665190107050574	28.875529539981365	21.815465204169524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	348.0
1	348.0
2	806.0
3	1264.0
4	1264.0
5	1264.0
6	2510.0
7	3756.0
8	3756.0
9	3756.0
10	4296.5
11	4837.0
12	4837.0
13	4837.0
14	5910.5
15	6984.0
16	6984.0
17	6984.0
18	12503.5
19	18023.0
20	18023.0
21	18023.0
22	27177.5
23	36332.0
24	36332.0
25	36332.0
26	52916.0
27	69500.0
28	69500.0
29	69500.0
30	86709.5
31	103919.0
32	103919.0
33	103919.0
34	115589.0
35	127259.0
36	127259.0
37	127259.0
38	135136.5
39	143014.0
40	143014.0
41	143014.0
42	152489.5
43	161965.0
44	161965.0
45	161965.0
46	173566.5
47	185168.0
48	185168.0
49	185168.0
50	181042.0
51	176916.0
52	176916.0
53	176916.0
54	158418.0
55	139920.0
56	139920.0
57	139920.0
58	121773.5
59	103627.0
60	103627.0
61	103627.0
62	87146.0
63	70665.0
64	70665.0
65	70665.0
66	54611.0
67	38557.0
68	38557.0
69	38557.0
70	28719.5
71	18882.0
72	18882.0
73	18882.0
74	13494.0
75	8106.0
76	8106.0
77	8106.0
78	5561.0
79	3016.0
80	3016.0
81	3016.0
82	2034.5
83	1053.0
84	1053.0
85	1053.0
86	685.5
87	318.0
88	318.0
89	318.0
90	191.0
91	64.0
92	64.0
93	64.0
94	42.5
95	21.0
96	21.0
97	21.0
98	13.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.335225592352473
2	0.010256273361999383
3	0.009132298199040547
4	0.011169503181903438
5	0.012785217478656765
6	0.08176919310525536
7	0.08078571483766638
8	0.10424869636443208
9	0.08528161548950172
10	0.09272795094410402
11	0.08851304408300838
12	0.09806683296815849
13	0.08443863411728258
14	0.08949652235059735
15	0.085984099966351
16	0.08752956581541939
17	0.0994015534741721
18	0.10164950380008979
19	0.0963106217760353
20	0.09448416213622719
21	0.08162869620988551
22	0.10115776466629528
23	0.09588913108992574
24	0.09202546646725474
25	0.09090149130429591
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1423519.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.199689746903445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63823247565935	41.752069656605535
2	13.7322606173443	15.160330512020526
3	4.73501371830406	7.841138645931475
4	2.0896579813491964	4.613938889904648
5	1.1498890498383898	3.1736759397220355
6	0.639126107620353	2.1167737709873706
7	0.42185208995697493	1.6300273139295476
8	0.2916243201136558	1.2878057594340364
9	0.21836416127390515	1.0848270558746118
>10	0.9626407630047759	9.37818206103384
>50	0.07188207434938679	2.766896684525893
>100	0.043033767274829625	4.40165324179543
>500	0.004367554259281193	1.6330142207938203
>1k	0.0016699472167839855	1.5057341284653398
>5k	2.5691495642830544E-4	0.8991150811839186
>10k+	1.2845747821415272E-4	0.7548170377920199
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10645	0.7477947256060509	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6359	0.44670987882845264	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6321	0.4440404378164254	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2936	0.2062494424029465	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2391	0.16796403841466112	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2056	0.1444308084402105	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1936	0.1360009947180192	No Hit
GTCCTACAGTGGACATTTCTAAATT	1780	0.12504223687917057	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1745	0.1225835412101981	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1476	0.10368670878295268	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	7.024844768492728E-5
5	0.0	0.0	0.0	0.0	7.024844768492728E-5
6	0.0	0.0	0.0	0.0	7.024844768492728E-5
7	0.0	0.0	0.0	0.0	7.024844768492728E-5
8	0.0	0.0	0.0	0.0	7.024844768492728E-5
9	0.0	0.0	0.0	7.024844768492728E-5	7.024844768492728E-5
10	0.0	0.0	0.0	7.024844768492728E-5	7.024844768492728E-5
11	0.0	0.0	0.0	7.024844768492728E-5	7.024844768492728E-5
12	0.0	0.0	0.0	7.024844768492728E-5	2.1074534305478185E-4
13	0.0	0.0	0.0	7.024844768492728E-5	2.1074534305478185E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1885	0.0	13.442216	1
CCATCGC	115	3.783498E-10	13.215043	19
GCGTGCG	115	3.783498E-10	13.215043	9
TAGGACC	375	0.0	13.170993	4
GGCGAGG	535	0.0	12.427698	19
ACTGCGT	55	0.00307231	12.088761	8
GTCTAGA	130	2.5047484E-9	11.724037	1
GGCGTGC	160	4.5474735E-11	11.279246	8
ACCATCG	135	4.7330104E-9	11.258051	18
GACTTAA	60	0.005757899	11.11341	1
AAGGCGT	215	0.0	11.044549	6
TTAGGAC	780	0.0	10.959591	3
CGTGCGC	130	3.272544E-8	10.959591	10
ACGAAGG	70	0.0014926032	10.855978	16
CCCCGTA	175	2.0008883E-11	10.855977	15
TGGCGAG	1025	0.0	10.842739	18
AGGCGTG	195	1.8189894E-12	10.716044	7
CTACACT	195	1.8189894E-12	10.716044	4
TATACTG	170	1.382432E-10	10.615761	5
GTATCAA	4140	0.0	10.584201	1
>>END_MODULE