FastQCFastQC Report
Thu 26 May 2016
SRR1512832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423519
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT131740.9254530498012321No Hit
TATCAACGCAGAGTACTTTTTTTTT98800.6940546631270815No Hit
GGTATCAACGCAGAGTACTTTTTTT78370.5505370845067751No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45410.3189982009372548No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30760.21608422507883632No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23350.1640301253443052No Hit
GTACTGGTTCACTATCGGTCAGTCA17650.12398851016389666No Hit
GTCCTACAGTGGACATTTCTAAATT17380.12209180207640362No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16340.11478596351717119No Hit
GTCCTAAAGTGTGTATTTCTCATTT15280.10733962806256889No Hit
CTGTAGGACGTGGAATATGGCAAGA14650.10291397585841847No Hit
CTTTAGGACGTGAAATATGGCGAGG14490.10179000069545964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.013.9212321
AATGCCG655.498602E-513.14188118
CGAAATC701.09827844E-412.20531913
AATCCCG550.003089208612.07991119
GTATAGA1459.458745E-1111.8068461
AACTAGC657.9470285E-411.705526
ATAGCCC1850.011.3104623
GTATCAA42500.011.2342521
TGCTAGA600.0058245311.0962482
GACACCG600.005826056511.0958585
TAGGACG10450.011.012434
ATGTCGA700.001502191410.84764917
TGGCGAG10200.010.79409418
TTACACC1502.0918378E-910.7788334
AGGACGT10700.010.7551315
GGCGAGG4900.010.65356619
GGACGTG10200.010.443166
GACGTGG4300.010.3903357
GTCCTAC8550.010.3453741
TTAGGAC7000.010.3263043
Produced by FastQC (version 0.11.5)