FastQCFastQC Report
Thu 26 May 2016
SRR1512831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355111
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74280.3153991467918073No Hit
GTATCAACGCAGAGTACTTTTTTTT64280.27293830312032No Hit
GTCCTAAAGTGTGTATTTCTCATTT60010.25480752287259495No Hit
CTGTAGGACGTGGAATATGGCAAGA56940.24177204386544837No Hit
CTTTAGGACGTGAAATATGGCGAGG47990.20376958877946727No Hit
GTCCTACAGTGTGCATTTCTCATTT37550.1594404679864346No Hit
GGTATCAACGCAGAGTACTTTTTTT36560.15523684446295738No Hit
TATCAACGCAGAGTACTTTTTTTTT36550.1551943836192859No Hit
CTGTAGGACCTGGAATATGGCGAGA25020.1062370308660611No Hit
CTGAAGGACCTGGAATATGGCGAGA24180.10267031999765616No Hit
GTACTGGTTCACTATCGGTCAGTCA24130.10245801577929872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11250.013.5939744
GGTATCA19950.012.9368031
CGTATAC550.00307192712.0895023
TCCAACG2050.012.04789218
GTATTAG1850.011.8401361
GGATCGT658.033947E-411.6909486
GGCGAGG13050.011.20966219
TATACCG600.00588320111.0818095
TAAGACG951.3626632E-510.998724
GCCGGTT2350.010.91269611
AGGACCT19500.010.9113195
CGGTTTG700.001493874510.85541814
TCCTATA4150.010.7577842
GTCCTAC35050.010.7327171
GGACCTG18600.010.673496
TGCCGGT2500.010.63763210
TATGTCG1803.6379788E-1110.55455216
TGTAGGA34400.010.5481922
GTAGGAC33450.010.3366163
GGCGTGC1752.3646862E-1010.31308658
Produced by FastQC (version 0.11.5)