##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512831_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2355111
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.92898551278475	31.0	30.0	34.0	22.0	34.0
2	29.061859080102806	31.0	30.0	34.0	22.0	34.0
3	29.22171948583315	31.0	30.0	34.0	22.0	34.0
4	32.61530942702913	35.0	33.0	37.0	25.0	37.0
5	32.82220328468595	37.0	35.0	37.0	26.0	37.0
6	32.71877928471312	37.0	33.0	37.0	25.0	37.0
7	32.677301834181065	37.0	33.0	37.0	25.0	37.0
8	32.56424346877918	37.0	33.0	37.0	23.0	37.0
9	33.88467889623886	39.0	34.0	39.0	23.0	39.0
10	33.76822663560231	39.0	34.0	39.0	22.0	39.0
11	33.821305237842296	39.0	34.0	39.0	22.0	39.0
12	33.71649276828141	39.0	34.0	39.0	20.0	39.0
13	33.69066511090136	39.0	34.0	39.0	19.0	39.0
14	34.55459126979578	39.0	34.0	41.0	17.0	41.0
15	34.73453650379961	39.0	34.0	41.0	19.0	41.0
16	34.67710821273392	39.0	34.0	41.0	19.0	41.0
17	34.460221620127456	39.0	34.0	41.0	17.0	41.0
18	34.464590840941256	39.0	34.0	41.0	17.0	41.0
19	34.577177041761516	39.0	34.0	41.0	18.0	41.0
20	34.55309792192385	39.0	34.0	41.0	18.0	41.0
21	34.444832111947164	39.0	34.0	41.0	18.0	41.0
22	34.46214169947828	39.0	34.0	41.0	18.0	41.0
23	34.13156577333298	39.0	34.0	41.0	10.0	41.0
24	34.202612106180986	39.0	34.0	41.0	2.0	41.0
25	33.93042196312616	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8356398094436805
1101	2	0.5280695937391968
1101	3	0.8349828565496509
1101	4	0.9355703505764552
1101	5	0.7671041709363209
1101	6	0.6898509131370147
1101	7	0.5023082064295536
1101	8	0.6919007324897777
1101	9	0.7094888444241292
1101	10	0.798289152905312
1101	11	0.8342385877226306
1101	12	0.8207748077059165
1101	13	1.2080156934506618
1101	14	1.2380585490376035
1101	15	1.1529841270079118
1101	16	0.9813444627483179
1101	17	0.3873222785769741
1101	18	0.8595968400519922
1101	19	0.21276597175527456
1101	20	-0.3677149121797214
1101	21	0.789877714780765
1101	22	0.8997727729008602
1101	23	0.43379599507035493
1101	24	0.9381016001358091
1101	25	0.5600230843348228
1102	1	0.9326689409377735
1102	2	0.8308759034120001
1102	3	0.8697416498984794
1102	4	1.103755543830296
1102	5	0.8772492323773804
1102	6	0.7560004060664696
1102	7	0.7480077077112313
1102	8	0.7694355204787939
1102	9	1.04174971566826
1102	10	0.9921254975355325
1102	11	0.8671613760021231
1102	12	0.9826012856849147
1102	13	1.0117187049351557
1102	14	1.148015742010351
1102	15	1.1201768707231636
1102	16	1.081560495071642
1102	17	1.2221995430294754
1102	18	1.3515313701498357
1102	19	1.11634178079386
1102	20	1.3204588404362667
1102	21	1.4184103810614417
1102	22	1.1738442900196446
1102	23	1.057125735030347
1102	24	0.6377671123423738
1102	25	0.9093253156422207
1103	1	0.5773964256049418
1103	2	0.694430071531233
1103	3	0.8890382311311704
1103	4	0.9613215540604401
1103	5	0.8193574552722325
1103	6	0.40937845628062774
1103	7	0.34937429282321375
1103	8	0.505821464755762
1103	9	0.5655007763348081
1103	10	0.7263518128806723
1103	11	0.565901198329037
1103	12	0.6714406749187134
1103	13	0.8816092707321772
1103	14	0.9271404385902429
1103	15	0.8264460228760129
1103	16	0.7267897794170324
1103	17	0.535536501852306
1103	18	0.8281973838669003
1103	19	0.8513658695130673
1103	20	1.0264750969986167
1103	21	0.7957310539504832
1103	22	0.8559833770213743
1103	23	0.22954901476984446
1103	24	0.5410452431924213
1103	25	0.8872175114786032
1104	1	0.8908206226630959
1104	2	0.8280988103657378
1104	3	0.8068379116796933
1104	4	0.9828274839480784
1104	5	0.7281996941039495
1104	6	0.6598236848220012
1104	7	0.8387514180938282
1104	8	0.7031481456326389
1104	9	1.0017523972449638
1104	10	0.9536034170947616
1104	11	0.7592055176024246
1104	12	1.0422923971183664
1104	13	0.9981144492966507
1104	14	1.211512081984445
1104	15	0.9240609892334319
1104	16	0.9765643824679628
1104	17	1.2165177299039343
1104	18	1.0774293646330264
1104	19	0.6741649100455618
1104	20	0.7674480883222259
1104	21	0.9761696597173071
1104	22	0.7135784194015642
1104	23	1.0836309256907484
1104	24	0.9811160901840665
1104	25	0.3284974322279268
1105	1	0.6801718151374807
1105	2	0.7466695398098011
1105	3	0.8138430522880711
1105	4	0.9883380231486143
1105	5	0.6903263470923733
1105	6	0.9406656017252288
1105	7	0.9345005215063225
1105	8	0.9004359648117273
1105	9	0.5706849106540517
1105	10	0.6325385652317621
1105	11	0.7173792120398517
1105	12	0.9224794454662018
1105	13	0.7980005088703876
1105	14	1.0042900096669385
1105	15	0.8325586512013032
1105	16	0.87995263678161
1105	17	1.231008877853725
1105	18	1.062114575315924
1105	19	0.7791533282207936
1105	20	0.9917014231886299
1105	21	0.9052580669722801
1105	22	0.9179808706229906
1105	23	1.0245041068762433
1105	24	1.110210968700578
1105	25	1.3197158725498923
1106	1	0.5900857026563102
1106	2	0.606955727093645
1106	3	0.517324654853418
1106	4	0.5607431377442254
1106	5	0.3826459601449912
1106	6	0.6175064255083029
1106	7	0.5935736239844971
1106	8	0.6429836366157318
1106	9	0.7409575529914747
1106	10	0.5899847840459742
1106	11	0.2761878229899537
1106	12	0.5193487598296542
1106	13	0.564749080988662
1106	14	0.8498551200306892
1106	15	0.6049728553649203
1106	16	0.7214821279595114
1106	17	0.9114439552417153
1106	18	0.758629545536408
1106	19	0.7602014299166697
1106	20	0.948252543068115
1106	21	0.8893727803007181
1106	22	0.711195582610344
1106	23	1.105282799736763
1106	24	0.7856923805340017
1106	25	0.9619193328441256
1107	1	0.878991431798525
1107	2	0.8409523349156096
1107	3	0.9002195384160245
1107	4	0.9846201390118878
1107	5	0.8604508160067752
1107	6	0.9300014370111924
1107	7	0.6454362407740177
1107	8	0.7860031152297537
1107	9	0.7523201756498139
1107	10	0.9763521017604191
1107	11	0.937636423863232
1107	12	0.9526983302320247
1107	13	0.7384582340005394
1107	14	0.9804689399283362
1107	15	0.8745684056254959
1107	16	0.9855919453218291
1107	17	1.2499896322688002
1107	18	1.2502268623118482
1107	19	1.094272571754317
1107	20	1.1998173172359117
1107	21	1.1050434103772488
1107	22	0.9883686599848645
1107	23	1.3704133516406074
1107	24	1.067597047419774
1107	25	1.3042121048052522
1108	1	0.8710774273607349
1108	2	0.8969877971409197
1108	3	0.9239247929206513
1108	4	1.1099281332730584
1108	5	0.942055649901512
1108	6	0.8914386618770749
1108	7	0.8860869719401379
1108	8	0.909979159625486
1108	9	0.9511352053241993
1108	10	0.9995870105985745
1108	11	0.7858799097567584
1108	12	0.8783931831076757
1108	13	0.9789193231306257
1108	14	1.089891559294209
1108	15	1.1102884438546567
1108	16	0.8483746539781905
1108	17	1.0927774982974228
1108	18	1.0851068503707992
1108	19	1.0566677534542208
1108	20	1.1431967773047091
1108	21	1.0370680187288528
1108	22	1.0348426547985312
1108	23	1.3526758340409373
1108	24	1.166185286383218
1108	25	1.2622508107169566
1109	1	0.8029835300262604
1109	2	0.7972299351872287
1109	3	0.8598126142892291
1109	4	0.9225564875015735
1109	5	0.7969442633312909
1109	6	0.9319709777786471
1109	7	0.8709977039369292
1109	8	0.798300483704061
1109	9	0.9071163833387672
1109	10	0.9206751587237818
1109	11	0.8512263535303219
1109	12	0.7615771296258274
1109	13	0.907155900287961
1109	14	1.040614625516831
1109	15	0.9953113728964098
1109	16	0.6044546792214831
1109	17	0.9774395498614723
1109	18	1.0434821372802574
1109	19	0.988544840845158
1109	20	0.8634140626523532
1109	21	0.8579339907586814
1109	22	0.9056022049390151
1109	23	1.2198079866199905
1109	24	0.932214342390786
1109	25	0.6731177006338456
1110	1	0.6403393983859935
1110	2	0.62495303929024
1110	3	0.5111481923318166
1110	4	0.4176176063545185
1110	5	0.4310264214598263
1110	6	0.46148218042451816
1110	7	0.33877534637279894
1110	8	0.40747171977308483
1110	9	0.5182894967121641
1110	10	0.5934323033131363
1110	11	0.5238469656355207
1110	12	0.4445400601231242
1110	13	0.4570641573716472
1110	14	0.4802931222796687
1110	15	0.6319578530336614
1110	16	0.6031529543219278
1110	17	0.6806439223298355
1110	18	0.038311945909413225
1110	19	0.39730408755085733
1110	20	0.49029746778219163
1110	21	0.6351943430664164
1110	22	0.6543293419696568
1110	23	0.7558135037766931
1110	24	0.6599799754984872
1110	25	0.780125086937538
1111	1	0.37204880030648013
1111	2	0.34213242747101447
1111	3	0.503373880353351
1111	4	0.3135223377605598
1111	5	0.3889935202400423
1111	6	0.30153869792460597
1111	7	0.2674137702052022
1111	8	0.38411215009199395
1111	9	0.4579775912387518
1111	10	0.45918595276139484
1111	11	0.2146976670688403
1111	12	-0.05195119538806381
1111	13	0.3253809341727987
1111	14	0.44503074096545703
1111	15	0.3851467039716425
1111	16	0.49925100762937547
1111	17	0.08886376651430083
1111	18	0.26470286886301864
1111	19	0.21639444008823006
1111	20	0.28959709093482644
1111	21	0.16995798406009754
1111	22	-0.07287301159071546
1111	23	0.2104380133715793
1111	24	0.2417737996801037
1111	25	0.3109481991814178
1112	1	0.1997658349439959
1112	2	0.20265200471704503
1112	3	0.14659614704711643
1112	4	0.263341812389541
1112	5	0.25466680901039496
1112	6	-0.020747613194153303
1112	7	-0.19556533274143106
1112	8	0.06267017485723869
1112	9	0.00467208794764673
1112	10	0.02149661101088185
1112	11	0.003934348361241291
1112	12	0.07301986693735074
1112	13	0.20121098305408935
1112	14	0.1080208163301748
1112	15	-0.1479446958553794
1112	16	0.09449783092730968
1112	17	0.30839649944024217
1112	18	0.12313706324023599
1112	19	0.28577087839975235
1112	20	0.0566443425726888
1112	21	0.1218767648914465
1112	22	0.019964451569592256
1112	23	0.2562249996681132
1112	24	0.29589920028766414
1112	25	0.19607778801083242
1113	1	-0.07033045606387134
1113	2	0.09030247511008582
1113	3	0.11692250811244875
1113	4	0.13237534238374593
1113	5	0.010146892808180041
1113	6	-0.33125849343078073
1113	7	-0.23202306014084684
1113	8	0.020187145191833622
1113	9	-0.005227634260855041
1113	10	0.07098224896461858
1113	11	-0.028948874589239892
1113	12	-0.11791003290761637
1113	13	-0.05565303637708752
1113	14	0.31692388447962117
1113	15	-0.15344549474655622
1113	16	-0.171203442639424
1113	17	0.0020361008957934246
1113	18	-0.13001414187601767
1113	19	-0.6081191594677122
1113	20	-0.3428889191715143
1113	21	-0.010523231425871415
1113	22	-0.4390585130294937
1113	23	-4.080560988726415
1113	24	-1.6625207249259688
1113	25	-0.8967945319652415
1114	1	-0.6973170183386657
1114	2	-0.9495455552950638
1114	3	-0.7721492609048752
1114	4	-0.7654354982696354
1114	5	-0.7232616723640035
1114	6	-1.2906275387669623
1114	7	-1.1700557020031148
1114	8	-1.118776481442751
1114	9	-1.2562444238089867
1114	10	-0.9465582386866132
1114	11	-1.0982699886212401
1114	12	-2.2218347888559613
1114	13	-1.3542209328038695
1114	14	-3.067301643209408
1114	15	-1.9850022841233113
1114	16	-1.932757318672202
1114	17	-1.8302203637878875
1114	18	-1.7537655922692394
1114	19	-1.7932894999714364
1114	20	-1.4125706555481585
1114	21	-1.2902302542099306
1114	22	-1.5378044011813188
1114	23	-5.725160946119498
1114	24	-3.1880307300169335
1114	25	-3.1464597633323272
1115	1	-1.6720505410029318
1115	2	-1.4772783648166765
1115	3	-1.1863216330776218
1115	4	-1.2376588297577733
1115	5	-1.127522894094298
1115	6	-1.4296002223158943
1115	7	-1.848738851296023
1115	8	-2.221983630473261
1115	9	-3.3702061595953303
1115	10	-3.9958561211730554
1115	11	-2.8481654177671203
1115	12	-3.148727664744303
1115	13	-2.4299890369608903
1115	14	-3.1894589710690973
1115	15	-2.8188626928090805
1115	16	-2.843027486277709
1115	17	-2.7259559941748144
1115	18	-5.276450918960972
1115	19	-3.207599337240996
1115	20	-3.4826856302680937
1115	21	-2.9959439030144637
1115	22	-2.6631959710002597
1115	23	-3.2671098811197936
1115	24	-2.997653573651178
1115	25	-3.0796776189387174
1116	1	-1.329619172139438
1116	2	-1.2308128254149011
1116	3	-1.7851433966045676
1116	4	-1.399199091718085
1116	5	-1.4587761659876257
1116	6	-3.6657504615388667
1116	7	-2.226236432226255
1116	8	-2.4968421676133765
1116	9	-3.5645274784670278
1116	10	-3.013414802735369
1116	11	-2.474038096012851
1116	12	-2.3257802396290046
1116	13	-3.80192183964483
1116	14	-6.8341077586870504
1116	15	-4.806486747381303
1116	16	-3.4886366411259964
1116	17	-4.5225734326391525
1116	18	-3.167525760953559
1116	19	-2.9655612604587738
1116	20	-3.7588551682059865
1116	21	-4.626694368982655
1116	22	-4.066295594579056
1116	23	-2.9984681146575767
1116	24	-4.001578499561688
1116	25	-3.9148973820758712
1201	1	0.5229673544700866
1201	2	0.6302981806466263
1201	3	0.9101201194318378
1201	4	0.7835454134954389
1201	5	0.2542650168942018
1201	6	0.7979762959259347
1201	7	0.8282536264275464
1201	8	0.7571890680815017
1201	9	1.2114483207985174
1201	10	1.103924572041457
1201	11	1.2833501461487913
1201	12	0.9643082986800167
1201	13	1.076664458544883
1201	14	1.1204950223703776
1201	15	1.1482212513375956
1201	16	1.1516249306527442
1201	17	1.3806637164563753
1201	18	1.0857745817686038
1201	19	1.3201746954836011
1201	20	1.311318204783987
1201	21	1.4386986597421867
1201	22	1.2125333093176351
1201	23	1.5710471857300234
1201	24	1.3891280412370932
1201	25	1.4674114110868004
1202	1	0.9951508895273555
1202	2	0.9723189585852445
1202	3	1.0104484109228906
1202	4	1.1633291531315564
1202	5	1.0003782700739237
1202	6	1.257199313512622
1202	7	1.2656344721165382
1202	8	1.2903428391703144
1202	9	1.634634872312688
1202	10	1.4253387819272305
1202	11	1.432892013477371
1202	12	1.6401763693824378
1202	13	1.5686104226881596
1202	14	2.01946823024614
1202	15	1.6806562709846844
1202	16	1.481395111382838
1202	17	1.7632957399278197
1202	18	1.7349788087366207
1202	19	1.9103952450600943
1202	20	1.6638331648784757
1202	21	1.824430275015196
1202	22	1.6773973951786303
1202	23	1.7831061158874206
1202	24	1.798198104314551
1202	25	2.0729195701071106
1203	1	1.1062257199712242
1203	2	1.1521828299384786
1203	3	1.1074745607736922
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2211	16	-0.23671435007769048
2211	17	0.22249655684140635
2211	18	-0.11243043095985428
2211	19	-0.02218635773427735
2211	20	-0.10504157740052733
2211	21	0.10846671729631652
2211	22	-0.004084243749815641
2211	23	0.18304748657347147
2211	24	-0.15049947470217262
2211	25	0.23279958371052345
2212	1	0.04295117984302976
2212	2	0.02646803136422804
2212	3	-0.2399916015217265
2212	4	0.012394969474726736
2212	5	-0.20224955387174504
2212	6	-0.34808771032869146
2212	7	-0.23260239339080613
2212	8	-0.20731850680505914
2212	9	-0.1585011098206479
2212	10	-0.4647770887509779
2212	11	-0.5612531990021523
2212	12	-0.05125658611747497
2212	13	-0.03564234055473747
2212	14	-0.027696168274253807
2212	15	-0.4116084115938179
2212	16	-0.48215149908914867
2212	17	-0.23596921674957372
2212	18	-0.011935375396141978
2212	19	-0.19760544591588314
2212	20	-0.11597773332860584
2212	21	-0.050777612687106455
2212	22	0.048154040313988844
2212	23	0.06422978031634585
2212	24	0.08198431775738868
2212	25	0.21520994394802528
2213	1	-0.37452410795560453
2213	2	-0.18705604873172987
2213	3	-0.5110349625660078
2213	4	-0.1969808466577163
2213	5	-0.370277933902301
2213	6	-0.21502793303318413
2213	7	-0.4235867733381582
2213	8	-0.28022383428650244
2213	9	-0.7827238339522822
2213	10	-0.38407199076885945
2213	11	-0.5861073929695308
2213	12	-0.6454821818797072
2213	13	-0.6393992076230859
2213	14	-0.603142484382424
2213	15	-0.5001690370892788
2213	16	-0.5286748037870694
2213	17	-0.6583710555176268
2213	18	-0.5143194932566431
2213	19	-0.5967304652719463
2213	20	-0.37101290935898135
2213	21	-0.5915790345198175
2213	22	-0.7125604063568787
2213	23	-0.4458158710989011
2213	24	-0.17839298676950932
2213	25	0.007977017149762844
2214	1	-2.8759188391162063
2214	2	-1.4931618751077202
2214	3	-1.6730413054344027
2214	4	-3.332091090621489
2214	5	-1.9049396574732853
2214	6	-1.6877879704711702
2214	7	-1.4780217693167756
2214	8	-1.2021076199362248
2214	9	-1.0987241554255078
2214	10	-1.1877374083163161
2214	11	-1.2066172957683676
2214	12	-1.1785129626444615
2214	13	-1.440456807084054
2214	14	-1.3931441036110286
2214	15	-1.8877044169377655
2214	16	-1.6115810878175623
2214	17	-3.721151454903623
2214	18	-3.4543414566395363
2214	19	-2.459114272274128
2214	20	-2.2587158467464334
2214	21	-1.973103695430808
2214	22	-2.9718536219669573
2214	23	-1.901275666779533
2214	24	-3.098424890911172
2214	25	-2.8268776793249444
2215	1	-1.9584003873687337
2215	2	-3.627440495699638
2215	3	-2.1330281874218002
2215	4	-3.748632504795939
2215	5	-2.2891177808253005
2215	6	-1.9763295136454673
2215	7	-2.1981039113319802
2215	8	-1.6640263200538215
2215	9	-3.984176152265917
2215	10	-2.3977841344228423
2215	11	-1.8331091403889594
2215	12	-1.904795727583572
2215	13	-2.0141070894074886
2215	14	-1.8141416903145995
2215	15	-1.960857095978426
2215	16	-1.9932512088639314
2215	17	-2.3364312142371446
2215	18	-1.83623119538305
2215	19	-2.103871859304739
2215	20	-1.9020719231161607
2215	21	-2.479979296427018
2215	22	-2.467253914396892
2215	23	-2.472328464509136
2215	24	-2.190656147664928
2215	25	-3.1863130415442527
2216	1	-3.2413169378173983
2216	2	-3.3466885840705523
2216	3	-4.551024922010036
2216	4	-5.188272879181241
2216	5	-4.982422084786261
2216	6	-3.913753422264193
2216	7	-5.577014097431551
2216	8	-3.393860533502945
2216	9	-4.040358840055362
2216	10	-3.085564785193398
2216	11	-3.2940441079358678
2216	12	-3.6634432107413843
2216	13	-3.2397284787968488
2216	14	-4.255259368266348
2216	15	-3.8037937270132822
2216	16	-4.186460418229718
2216	17	-3.724841750875342
2216	18	-3.3743066472512098
2216	19	-4.115405161102164
2216	20	-4.646034319368038
2216	21	-4.333331259192025
2216	22	-4.003426453527808
2216	23	-4.117091021707239
2216	24	-4.5514697773353205
2216	25	-4.51493538392123
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117748.0
3	2.0
4	1060.0
5	6538.0
6	7506.0
7	6881.0
8	6003.0
9	5669.0
10	5076.0
11	4602.0
12	4446.0
13	4438.0
14	4398.0
15	4349.0
16	4584.0
17	4964.0
18	5638.0
19	6893.0
20	8565.0
21	11164.0
22	14037.0
23	16722.0
24	19782.0
25	22023.0
26	24744.0
27	27215.0
28	31146.0
29	36269.0
30	43713.0
31	54535.0
32	68511.0
33	88118.0
34	113213.0
35	148844.0
36	204799.0
37	314943.0
38	905973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18381719509296	18.874656073430078	12.65734986737152	24.28417686410544
2	14.332747316211442	19.97273746432939	43.29460524527789	22.399909974181277
3	19.402749933861113	25.776797502052112	28.81294394267595	26.007508621410825
4	11.863283243886883	16.41112357708801	38.73267727001334	32.992915909011764
5	12.539159127150116	39.40649265441235	35.267186594275984	12.787161624161559
6	31.0551420154654	36.80197048689317	18.9888845636694	13.15400293397203
7	28.411318613837405	31.853989286541456	21.811938643032747	17.922753456588396
8	25.047353073699064	37.570455762707546	20.767383338859275	16.614807824734118
9	27.834060058988026	14.829410714741057	20.26026128124708	37.076267945023844
10	15.727795108931259	29.061780131414533	34.03296413726273	21.17746062239147
11	34.23677006775775	22.34611917595894	25.224006891800517	18.19310386448279
12	24.24443870160429	24.759551627755148	31.743563107390354	19.252446563250206
13	29.503039070607922	22.40336044456873	25.85734430022329	22.23625618460006
14	20.743882614192973	23.14948415270355	27.84381142620356	28.262821806899925
15	22.89799182996258	32.360683522397906	23.177933759118194	21.56339088852132
16	21.316745383354448	28.330485139312174	29.057783984635478	21.294985492697897
17	20.95339986245321	29.359098277944824	28.257703852755228	21.429798006846738
18	20.719128079200917	27.391408395565925	31.290585433386653	20.598878091846508
19	23.314942112495444	26.846762069796533	28.070519939832188	21.767775877875835
20	23.932704662076095	27.222539339414897	28.745240231503576	20.09951576700543
21	23.119953422748058	26.811795604059363	27.179866387883116	22.888384585309467
22	23.31408795124031	28.9018230476167	27.322633393961315	20.461455607181676
23	21.793403256676076	28.431544645092355	28.447143299782766	21.327908798448806
24	23.006861014620934	26.883803916851797	29.259976904693396	20.849358163833873
25	22.196769009830845	27.82599012878145	28.862242822796198	21.11499803859151
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	308.0
1	308.0
2	722.0
3	1136.0
4	1136.0
5	1136.0
6	1853.0
7	2570.0
8	2570.0
9	2570.0
10	3084.5
11	3599.0
12	3599.0
13	3599.0
14	5546.5
15	7494.0
16	7494.0
17	7494.0
18	15214.5
19	22935.0
20	22935.0
21	22935.0
22	39703.0
23	56471.0
24	56471.0
25	56471.0
26	86877.5
27	117284.0
28	117284.0
29	117284.0
30	154967.5
31	192651.0
32	192651.0
33	192651.0
34	218730.5
35	244810.0
36	244810.0
37	244810.0
38	259995.5
39	275181.0
40	275181.0
41	275181.0
42	288750.5
43	302320.0
44	302320.0
45	302320.0
46	312772.5
47	323225.0
48	323225.0
49	323225.0
50	304721.5
51	286218.0
52	286218.0
53	286218.0
54	247535.0
55	208852.0
56	208852.0
57	208852.0
58	175222.5
59	141593.0
60	141593.0
61	141593.0
62	115153.5
63	88714.0
64	88714.0
65	88714.0
66	66881.5
67	45049.0
68	45049.0
69	45049.0
70	33090.0
71	21131.0
72	21131.0
73	21131.0
74	15041.5
75	8952.0
76	8952.0
77	8952.0
78	6109.0
79	3266.0
80	3266.0
81	3266.0
82	2130.5
83	995.0
84	995.0
85	995.0
86	624.5
87	254.0
88	254.0
89	254.0
90	158.0
91	62.0
92	62.0
93	62.0
94	45.0
95	28.0
96	28.0
97	28.0
98	20.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3376910897193381
2	0.009808454888113553
3	0.00925646392038422
4	0.010912436823572222
5	0.012568409726760224
6	0.08436969637524515
7	0.08475184396828854
8	0.10916682907939372
9	0.08878562411707983
10	0.09557935910451779
11	0.09214003076712733
12	0.1028826242160136
13	0.08870070242973686
14	0.09281940426587112
15	0.09014437111456743
16	0.09163050064306948
17	0.10432629290084416
18	0.10666163930277597
19	0.10012266937736691
20	0.09842423563050744
21	0.0862379734967906
22	0.10666163930277597
23	0.0995282175659661
24	0.09562181994818927
25	0.09383846451398682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2355111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.62055819872712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.85583601631039	39.60191153428218
2	14.18189660609063	15.208824246704264
3	5.224334149972356	8.403951400145708
4	2.443114883616099	5.240047352124538
5	1.3238608083001544	3.549307775923618
6	0.7714417345777236	2.48190818555111
7	0.4873004937905338	1.8290527141294644
8	0.3355396592721624	1.4393459062387242
9	0.24999544294671533	1.2064405678146821
>10	1.0233614557345176	9.33827971956179
>50	0.05599535026004086	2.072749408687495
>100	0.03917625856684897	4.09131086450678
>500	0.005032057405818515	1.8367150415683524
>1k	0.002795587447676011	2.6058354500201624
>5k	3.1949570830582983E-4	1.0943198327410741
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7428	0.3153991467918073	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6428	0.27293830312032	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6001	0.25480752287259495	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5694	0.24177204386544837	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4799	0.20376958877946727	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3755	0.1594404679864346	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3656	0.15523684446295738	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3655	0.1551943836192859	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2502	0.1062370308660611	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2418	0.10267031999765616	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2413	0.10245801577929872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	4.246084367148725E-5	0.0
12	0.0	0.0	0.0	4.246084367148725E-5	4.246084367148725E-5
13	0.0	0.0	0.0	4.246084367148725E-5	4.246084367148725E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1125	0.0	13.593974	4
GGTATCA	1995	0.0	12.936803	1
CGTATAC	55	0.003071927	12.089502	3
TCCAACG	205	0.0	12.047892	18
GTATTAG	185	0.0	11.840136	1
GGATCGT	65	8.033947E-4	11.690948	6
GGCGAGG	1305	0.0	11.209662	19
TATACCG	60	0.005883201	11.081809	5
TAAGACG	95	1.3626632E-5	10.99872	4
GCCGGTT	235	0.0	10.912696	11
AGGACCT	1950	0.0	10.911319	5
CGGTTTG	70	0.0014938745	10.855418	14
TCCTATA	415	0.0	10.757784	2
GTCCTAC	3505	0.0	10.732717	1
GGACCTG	1860	0.0	10.67349	6
TGCCGGT	250	0.0	10.637632	10
TATGTCG	180	3.6379788E-11	10.554552	16
TGTAGGA	3440	0.0	10.548192	2
GTAGGAC	3345	0.0	10.336616	3
GGCGTGC	175	2.3646862E-10	10.3130865	8
>>END_MODULE