FastQCFastQC Report
Thu 26 May 2016
SRR1512831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355111
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82660.3509813337885136No Hit
GTCCTACAGTGGACATTTCTAAATT67490.28656823393886743No Hit
TATCAACGCAGAGTACTTTTTTTTT56220.23871486312110132No Hit
CTGTAGGACGTGGAATATGGCAAGA55600.2360822908134691No Hit
GTCCTAAAGTGTGTATTTCTCATTT53870.2287365648583018No Hit
CTTTAGGACGTGAAATATGGCGAGG50210.21319589607453746No Hit
GGTATCAACGCAGAGTACTTTTTTT46740.1984619833205314No Hit
GTCCTACAGTGTGCATTTCTCATTT35400.15031138659706483No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26690.11332799175919947No Hit
CTGAAGGACCTGGAATATGGCGAGA25920.11005850679649495No Hit
CTGTAGGACCTGGAATATGGCGAGA25340.10759577786354868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT456.7062076E-414.7945584
TCGCAAG400.00531123914.23740318
TGTCGAG1550.014.08431218
GTCGAGG1351.8189894E-1213.35798519
TAGGACC11700.012.6810494
TCGAATA604.117422E-412.65788911
GGTATCA19100.012.3506611
GGCGAGG13600.011.86419
CGAAATC2300.011.14397213
TATACCG600.005827744511.0959185
GCCGGTT1803.6379788E-1211.07565211
TGTAGGA34750.011.0576152
CTAGGAC4650.011.0452553
TCCAACG2250.010.96807518
GTATCAA41800.010.8773591
CGGTAGG700.001476265810.8708571
CCAACGT700.001503475210.84708519
AGGACGT31050.010.8432155
GGACGTG30800.010.8383616
TGGCGAG31450.010.77425218
Produced by FastQC (version 0.11.5)