##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512831_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2355111
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.191455944114736	31.0	30.0	34.0	26.0	34.0
2	30.221829884026697	31.0	30.0	34.0	26.0	34.0
3	30.39821053020431	31.0	30.0	34.0	26.0	34.0
4	33.970082514157504	37.0	35.0	37.0	30.0	37.0
5	33.54937707819292	37.0	35.0	37.0	28.0	37.0
6	33.41318434672506	37.0	35.0	37.0	28.0	37.0
7	33.24974619030695	37.0	35.0	37.0	27.0	37.0
8	33.19642683508336	37.0	35.0	37.0	26.0	37.0
9	34.52171171549876	39.0	35.0	39.0	27.0	39.0
10	34.44484187794121	39.0	35.0	39.0	27.0	39.0
11	34.42430017098982	39.0	35.0	39.0	26.0	39.0
12	34.29271444106031	39.0	35.0	39.0	25.0	39.0
13	34.28429105889276	39.0	35.0	39.0	25.0	39.0
14	35.351794034336386	40.0	36.0	41.0	25.0	41.0
15	35.360398724306414	40.0	36.0	41.0	25.0	41.0
16	35.356448167411216	40.0	36.0	41.0	25.0	41.0
17	35.35255620648029	40.0	36.0	41.0	25.0	41.0
18	35.27934139834598	40.0	36.0	41.0	25.0	41.0
19	35.29626798906718	40.0	36.0	41.0	25.0	41.0
20	35.269324885323876	40.0	36.0	41.0	25.0	41.0
21	35.23584493469735	40.0	36.0	41.0	25.0	41.0
22	35.19742466490964	40.0	36.0	41.0	25.0	41.0
23	35.04265404051019	40.0	35.0	41.0	24.0	41.0
24	34.99858860155636	40.0	35.0	41.0	24.0	41.0
25	34.94402684204694	40.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6404463619683369
1101	2	0.5569926408802317
1101	3	0.49588411559290435
1101	4	0.5421288192043363
1101	5	0.5135234248001694
1101	6	0.554242272488132
1101	7	0.6990043032138047
1101	8	0.6042866698022564
1101	9	0.8915037429308938
1101	10	0.6025911469025047
1101	11	0.8141528121485848
1101	12	0.9521890780612949
1101	13	1.048320769230557
1101	14	0.9758773121111162
1101	15	0.7575708100381604
1101	16	1.0337108382183757
1101	17	1.0818771982484137
1101	18	0.9307924931409843
1101	19	0.8880875651994558
1101	20	1.0082868081161465
1101	21	1.0672892613003668
1101	22	1.0464633636130358
1101	23	1.1035844304277234
1101	24	1.1087824878324284
1101	25	1.219380946951361
1102	1	0.7674186818937834
1102	2	0.8351617135905833
1102	3	0.4842267966214955
1102	4	0.663949334544256
1102	5	0.8648745902389408
1102	6	0.7898016134492352
1102	7	0.7971278619463078
1102	8	0.8441256191396036
1102	9	0.9887500245477128
1102	10	0.8992867677005449
1102	11	1.0465601151151347
1102	12	0.9576157987669163
1102	13	1.0897673642533192
1102	14	1.1814839281703868
1102	15	1.2431726547354884
1102	16	1.2433343407091542
1102	17	1.2801337751104498
1102	18	0.6997623503120138
1102	19	0.967121924086392
1102	20	1.3090293317773387
1102	21	1.1358188071228312
1102	22	0.8681153413793083
1102	23	1.0361087427602698
1102	24	1.0937066568677736
1102	25	1.1892929917715236
1103	1	0.5850900738219238
1103	2	0.7783822101531079
1103	3	0.5234013628863501
1103	4	0.4486384119174289
1103	5	0.5756329780242808
1103	6	0.5921562536502805
1103	7	0.6556660666903653
1103	8	0.7720272552565888
1103	9	0.8102113106987758
1103	10	0.7522889087275644
1103	11	0.938608265893329
1103	12	1.0176411470688365
1103	13	1.041907513898522
1103	14	1.0689035925874322
1103	15	1.0937597106535009
1103	16	1.1184725276646645
1103	17	1.0844643598046062
1103	18	1.0691237407467327
1103	19	1.0055993329714923
1103	20	0.8436937635037012
1103	21	0.9984675066046194
1103	22	0.9535618176994873
1103	23	1.0692854359689221
1103	24	0.959177273495456
1103	25	0.9316144512878566
1104	1	0.6609138857573882
1104	2	0.7777101933234718
1104	3	0.6578729044345231
1104	4	0.5326613857101634
1104	5	0.6522651580301755
1104	6	0.6167008683328064
1104	7	0.6121394114141268
1104	8	0.6566657575077741
1104	9	0.7073774310329171
1104	10	0.7726275050332845
1104	11	0.7713971218229361
1104	12	0.8401674851197427
1104	13	0.7578795312846935
1104	14	0.9429331335011497
1104	15	0.9337028327073398
1104	16	0.9691233961385848
1104	17	0.8730823704121704
1104	18	0.48554182147779557
1104	19	0.9672150673481426
1104	20	0.8374716946379763
1104	21	0.8392774329749528
1104	22	0.7631278311787852
1104	23	0.780877517579313
1104	24	0.9095843793429736
1104	25	0.8004028174660718
1105	1	0.6211203941131167
1105	2	0.6004491952122706
1105	3	0.638320594396383
1105	4	0.5493196840341668
1105	5	0.5390744158631477
1105	6	0.6780523277467267
1105	7	0.6861353980763951
1105	8	0.7069766380673528
1105	9	0.7781877658687009
1105	10	0.5341126942223511
1105	11	0.848338976960278
1105	12	0.8608009869562494
1105	13	0.9034312034413432
1105	14	0.9670528830402176
1105	15	1.0681250906113817
1105	16	1.0123884018096092
1105	17	0.7020973404045847
1105	18	0.6142469798243653
1105	19	0.8603170492297636
1105	20	0.9312350741156408
1105	21	0.8108736505225878
1105	22	0.7883344059804998
1105	23	0.9755801544450478
1105	24	0.9524726651462387
1105	25	0.835216471134558
1106	1	-0.015565847410115907
1106	2	0.29264885576905897
1106	3	-1.4590839614143114
1106	4	-0.3128301627097585
1106	5	-0.019485647555214314
1106	6	0.08595011927972251
1106	7	0.2199578296401441
1106	8	0.2968489221863848
1106	9	0.4179546258997888
1106	10	0.2501446060565158
1106	11	0.2922631967847735
1106	12	0.5009962487581561
1106	13	0.5182843026115123
1106	14	0.49248087503544014
1106	15	0.45859400801403183
1106	16	0.3708758976932245
1106	17	0.5795298801639959
1106	18	0.49745035220310285
1106	19	0.5282982263735221
1106	20	0.47642810822834747
1106	21	0.5006347736951753
1106	22	0.3394831932682152
1106	23	0.44020817505005994
1106	24	0.4707240706765745
1106	25	0.5099058984768661
1107	1	0.6930252249406976
1107	2	0.7497675878691545
1107	3	0.7109345840573091
1107	4	0.5060614225759394
1107	5	0.6228621445946558
1107	6	0.5314499080021946
1107	7	0.5402781791870694
1107	8	0.5378052170828624
1107	9	0.6981001139508649
1107	10	0.5887247624018883
1107	11	0.6947891369697601
1107	12	0.9084408470961094
1107	13	0.8449038035270462
1107	14	0.9112763583914614
1107	15	0.8864455209958564
1107	16	0.963002141883095
1107	17	0.9183490442092577
1107	18	0.9449635863925039
1107	19	0.9819235432898097
1107	20	0.8993609630572124
1107	21	0.9787316033585398
1107	22	0.9552474310258603
1107	23	1.0278306866703204
1107	24	1.0787294347781753
1107	25	1.0971572072237592
1108	1	0.5698770891692533
1108	2	0.5244903930341032
1108	3	0.5729464716332764
1108	4	0.46374952742542774
1108	5	0.697369186691283
1108	6	0.6463022489813639
1108	7	0.7353708225241462
1108	8	0.6123935741200555
1108	9	0.8622220685392605
1108	10	0.7197816115704683
1108	11	0.686154158624042
1108	12	0.7227810153400611
1108	13	0.7320782095938085
1108	14	0.8269070949934374
1108	15	0.9243023599165312
1108	16	0.8490417589677008
1108	17	0.8412644298871328
1108	18	0.9039582998074636
1108	19	0.8829067300334685
1108	20	0.9856937594891519
1108	21	0.9756435849479459
1108	22	0.804074045294513
1108	23	0.8772518468228796
1108	24	0.861008681423094
1108	25	0.7951025569002823
1109	1	0.30356220480767604
1109	2	0.31589616634836304
1109	3	0.4277631048626276
1109	4	0.4220175415168086
1109	5	0.09782733508711772
1109	6	0.3538233289968389
1109	7	0.3223070717809833
1109	8	0.4393882556760218
1109	9	0.5761632662953744
1109	10	0.32529014750156193
1109	11	0.45831045944436255
1109	12	0.6166691569926357
1109	13	0.4994002587752533
1109	14	0.7530739623955824
1109	15	0.6359143844043089
1109	16	0.628994798861747
1109	17	0.6967537850477683
1109	18	0.8073928852893957
1109	19	0.6980060557352274
1109	20	0.7277365296778555
1109	21	0.8085105116919067
1109	22	0.662335754143534
1109	23	0.6790512960348494
1109	24	0.7084859846430405
1109	25	0.6424620553154625
1110	1	0.387357824458455
1110	2	0.2065980069168596
1110	3	0.26762128528764606
1110	4	0.2690408775798687
1110	5	0.3203267547376498
1110	6	0.4033111351597043
1110	7	0.2991575224626146
1110	8	0.31584730401903016
1110	9	0.22869486889915436
1110	10	0.33155488004790357
1110	11	0.3085710325545037
1110	12	0.44214981009767484
1110	13	0.46708713303305416
1110	14	0.5401537309636879
1110	15	0.5608896115356004
1110	16	0.3004609337591617
1110	17	0.4790554005830714
1110	18	0.3352057620931248
1110	19	0.4579627994790343
1110	20	0.4938988303698082
1110	21	0.5681726909217986
1110	22	0.6415429283275955
1110	23	0.6698343509640452
1110	24	0.5448855034403479
1110	25	0.5272641717003168
1111	1	0.16374269666979657
1111	2	0.03197440836343901
1111	3	0.0434032785530718
1111	4	0.2787895700844416
1111	5	0.4399777819506241
1111	6	0.3685988088509973
1111	7	0.30958263581455725
1111	8	0.2416169076693464
1111	9	0.336998764891149
1111	10	0.23291611869110085
1111	11	0.2049952222421183
1111	12	0.46449008353634014
1111	13	0.3919008291694439
1111	14	0.35981417044241226
1111	15	0.30364031464282704
1111	16	0.16845692517671296
1111	17	0.3828881136124238
1111	18	0.34762240660117527
1111	19	0.14331939949423855
1111	20	0.4679216629349341
1111	21	0.4938671062101747
1111	22	0.22665533081254097
1111	23	0.35473543024633614
1111	24	0.43398582529592744
1111	25	0.3849933843821205
1112	1	-0.103826991692749
1112	2	-0.3368060697098052
1112	3	-0.13478899894270668
1112	4	-0.04788924105405812
1112	5	0.02641778505947201
1112	6	-0.047162321607316926
1112	7	0.11235799259478085
1112	8	0.11315524484094652
1112	9	0.1637482317521588
1112	10	-0.16776313217536654
1112	11	-0.03805547573251289
1112	12	0.2931586845073184
1112	13	0.03673570882404675
1112	14	-0.09166945426265727
1112	15	-0.21280293447303933
1112	16	-0.2770909954045919
1112	17	-0.012578407420335225
1112	18	0.16218459257285645
1112	19	0.013010297333124754
1112	20	-0.04515317851570444
1112	21	-0.04603321934646232
1112	22	0.11109374042704445
1112	23	0.29075916724411854
1112	24	0.2450085751917115
1112	25	0.2331985004382986
1113	1	-0.7416265131113171
1113	2	-0.6264650113903301
1113	3	-0.6675343305139307
1113	4	-2.2768519826220412
1113	5	-0.9233590638637494
1113	6	-0.6960875221252323
1113	7	-0.5606963858505836
1113	8	-0.5754251309768819
1113	9	-0.38837617431054383
1113	10	-0.47249854672693203
1113	11	-0.5755165948274197
1113	12	-0.319621188959033
1113	13	-0.5332893278423683
1113	14	-0.49964847580160665
1113	15	-0.5902249613270527
1113	16	-0.7739503919523045
1113	17	-0.944844794872651
1113	18	-0.7141416965127547
1113	19	-0.6193239965667559
1113	20	-0.33164229242026266
1113	21	-0.4547729102744782
1113	22	-0.48309283714100815
1113	23	-0.29341602190491045
1113	24	-0.22182314264332348
1113	25	-0.4719983794362008
1114	1	-1.0185022589908996
1114	2	-2.310411511048912
1114	3	-1.5425028259195024
1114	4	-1.20597056015378
1114	5	-1.0729985744810548
1114	6	-0.7245205478634205
1114	7	-0.8319580351637441
1114	8	-0.8861716029793811
1114	9	-1.1085494119073331
1114	10	-1.2089708506058443
1114	11	-1.094283878033707
1114	12	-1.078724797930363
1114	13	-1.2480761292713822
1114	14	-1.352486858783422
1114	15	-1.4011832121624224
1114	16	-1.6458573646835788
1114	17	-1.8202173468585556
1114	18	-1.4428978493268048
1114	19	-1.8753744299192903
1114	20	-1.3150009968626932
1114	21	-1.6631239103108229
1114	22	-1.2018607408363735
1114	23	-1.0498973258302087
1114	24	-1.1342106753039545
1114	25	-1.7858785429025659
1115	1	-0.8512067077494478
1115	2	-1.1028940197288044
1115	3	-0.5922829308323223
1115	4	-0.349444942540309
1115	5	-0.6911749629596855
1115	6	-0.509396126098153
1115	7	-0.45961785456888293
1115	8	-0.7500513390614927
1115	9	-1.5161390856138084
1115	10	-1.168686330315623
1115	11	-1.5001054531693327
1115	12	-2.086699358810151
1115	13	-2.1126471162549265
1115	14	-1.5170439029864156
1115	15	-1.92275862709225
1115	16	-1.6959467471920249
1115	17	-1.7167233404047124
1115	18	-1.9422848425620316
1115	19	-2.511099094803761
1115	20	-2.550941378529515
1115	21	-1.8474819675583518
1115	22	-1.8997144207528578
1115	23	-1.8942852509746118
1115	24	-2.3632353019382535
1115	25	-2.400788078262238
1116	1	-1.5329904291659382
1116	2	-1.6507112441908198
1116	3	-1.5512650035694513
1116	4	-1.264637222793283
1116	5	-1.7019354486614127
1116	6	-2.395032313505517
1116	7	-1.8742511035233633
1116	8	-2.2365637351165475
1116	9	-3.24971639399293
1116	10	-1.8995405586929408
1116	11	-3.0967582323645715
1116	12	-5.28146806588191
1116	13	-4.024468647848128
1116	14	-3.4130879845928312
1116	15	-4.252780765629058
1116	16	-3.76231822249051
1116	17	-3.587376510044873
1116	18	-3.4873634867016747
1116	19	-3.956807468019832
1116	20	-5.677697697418754
1116	21	-4.464392197288603
1116	22	-3.7096026626257377
1116	23	-4.52131686371526
1116	24	-4.321478825190489
1116	25	-3.6873931662343082
1201	1	0.4001333260490405
1201	2	0.5828106153246075
1201	3	0.5778159731025632
1201	4	0.5083760101765193
1201	5	-0.041236018458747026
1201	6	0.4594195575241855
1201	7	0.6699823761482406
1201	8	0.611120841831557
1201	9	0.5041710078140724
1201	10	0.6071922371058776
1201	11	0.40442550834684
1201	12	0.8738853070458745
1201	13	0.8616434864459137
1201	14	0.7144695210191259
1201	15	0.6461996063187385
1201	16	0.6212845294051519
1201	17	0.43435085300976084
1201	18	0.8571175402092592
1201	19	0.9997295699836641
1201	20	0.9160058155149073
1201	21	0.5867729160309025
1201	22	0.7104831368305753
1201	23	0.7379675234040519
1201	24	0.4120343362195342
1201	25	0.6715368844424532
1202	1	0.8785340115922011
1202	2	0.8730334031483586
1202	3	0.8927184060773357
1202	4	0.7998127806811084
1202	5	0.8986324791855367
1202	6	0.9451275874570157
1202	7	0.9649667685607923
1202	8	0.9138597713007357
1202	9	1.191639289794857
1202	10	1.2632490788415467
1202	11	1.2071149527575358
1202	12	1.2141214253830697
1202	13	1.1486480705894664
1202	14	1.3600671246632956
1202	15	1.3516585238993173
1202	16	1.387555787615291
1202	17	1.2332434992103174
1202	18	1.3111689885303335
1202	19	1.3586536825909477
1202	20	1.3120811783660002
1202	21	1.4074214017348226
1202	22	1.3829517362770432
1202	23	1.4836623613815902
1202	24	1.4291607343056683
1202	25	1.4719501684247192
1203	1	0.803467519431063
1203	2	0.8445799539318877
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2211	10	-0.19528130921011666
2211	11	-0.10928673066542416
2211	12	-0.26471932606406057
2211	13	-0.054146475290181684
2211	14	-0.23568508412082423
2211	15	-0.13358185628787567
2211	16	-0.09508745566624555
2211	17	-0.2768195760123291
2211	18	-0.2360165387083839
2211	19	-0.1019818697999213
2211	20	-0.013757433376866857
2211	21	0.04702692032969935
2211	22	0.05660299636447519
2211	23	-0.16260537797125352
2211	24	0.044723363166170316
2211	25	-0.1847955681082567
2212	1	-0.1609996853848692
2212	2	-0.039762608228066654
2212	3	-0.0740852218990824
2212	4	-0.08772294691923577
2212	5	-0.2842108693334069
2212	6	0.023856103524408923
2212	7	-0.0813101136216332
2212	8	0.028616867685713032
2212	9	0.01678006292999612
2212	10	-0.23491066881120304
2212	11	0.0012794667343740684
2212	12	0.17489884386509402
2212	13	0.17690713722546292
2212	14	0.20028245534025046
2212	15	0.09012984531155155
2212	16	0.006037456040246525
2212	17	-0.24858444953407144
2212	18	-0.24613750955229818
2212	19	0.10038378420143346
2212	20	3.743355004601767E-4
2212	21	0.09820524523581042
2212	22	0.08367981695906934
2212	23	-0.14153120443342715
2212	24	-0.1630872026815524
2212	25	-0.1440249493893333
2213	1	-0.594012401450513
2213	2	-0.23086341344022188
2213	3	-0.3110656705436945
2213	4	-0.6111039134973879
2213	5	-0.3506241160440311
2213	6	-0.4321376019999832
2213	7	-0.7844301662701199
2213	8	-0.6988520990588398
2213	9	-0.5659188538239164
2213	10	-0.6573084630291177
2213	11	-0.35996371819863526
2213	12	-0.7203145177107544
2213	13	-0.5251428430140166
2213	14	-0.654738044751042
2213	15	-0.9520772021437338
2213	16	-1.3434408547707193
2213	17	-1.0177040491929432
2213	18	-0.7244949772941212
2213	19	-0.7618716938528394
2213	20	-0.8758888641778242
2213	21	-0.6889583926405933
2213	22	-0.8567251811804439
2213	23	-0.978314972382563
2213	24	-0.7182694558888798
2213	25	-0.7628906668578566
2214	1	-1.352027583053232
2214	2	-0.6338421119837463
2214	3	-0.8118950062210537
2214	4	-0.9032530865638648
2214	5	-1.2616549181612484
2214	6	-1.6951150075331185
2214	7	-1.1635925624437533
2214	8	-1.0897908246013728
2214	9	-0.9829247874367297
2214	10	-1.1678826782667073
2214	11	-1.349968243734999
2214	12	-1.034448870980178
2214	13	-1.414081109478552
2214	14	-1.0759637592875606
2214	15	-1.141620217871008
2214	16	-1.212510800701537
2214	17	-1.149976787788944
2214	18	-1.2518944902206641
2214	19	-1.2407895449881963
2214	20	-1.397239105104802
2214	21	-1.5402212723289423
2214	22	-1.8803163929802906
2214	23	-1.9748868637602044
2214	24	-1.7058285310424424
2214	25	-2.054934412127693
2215	1	-1.6681973504855847
2215	2	-1.0123982948237682
2215	3	-1.032703680824845
2215	4	-1.1472301790980879
2215	5	-1.8281307195614396
2215	6	-1.641783336662602
2215	7	-1.9602358864972764
2215	8	-1.7729390386121189
2215	9	-1.7786576178523532
2215	10	-1.9689163122514444
2215	11	-1.4216962466493044
2215	12	-1.6039016618055584
2215	13	-1.917288845108807
2215	14	-2.0594206644813013
2215	15	-1.9924323491256288
2215	16	-1.9074525077601407
2215	17	-2.0892444320382566
2215	18	-1.7217812020100212
2215	19	-1.9977542876039749
2215	20	-2.0870835013105733
2215	21	-1.7430828432291179
2215	22	-1.9118736618718373
2215	23	-2.3295935185625183
2215	24	-2.182605833246839
2215	25	-2.4825373360636647
2216	1	-1.684399553476343
2216	2	-1.1833614939221668
2216	3	-1.1824431954482115
2216	4	-1.4494300445364843
2216	5	-2.2770635084919597
2216	6	-2.444044766231979
2216	7	-2.5741358893870228
2216	8	-2.4236485809267805
2216	9	-4.568980239026896
2216	10	-2.4529240869217013
2216	11	-2.6709539434220915
2216	12	-3.0646096116194883
2216	13	-2.9441453334656487
2216	14	-3.1126944470820206
2216	15	-2.924069465009424
2216	16	-2.3694281761078884
2216	17	-2.7068331786176785
2216	18	-3.1572294045886835
2216	19	-3.4418757297625895
2216	20	-2.769729672147271
2216	21	-3.3173950682321944
2216	22	-2.7411984949576578
2216	23	-3.690192851659642
2216	24	-3.0480684971142225
2216	25	-3.2596520953730383
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70342.0
3	0.0
4	1231.0
5	10354.0
6	11850.0
7	9235.0
8	7406.0
9	6274.0
10	5358.0
11	4786.0
12	4249.0
13	4071.0
14	3898.0
15	3887.0
16	4030.0
17	4204.0
18	4710.0
19	5580.0
20	7347.0
21	9698.0
22	12044.0
23	15177.0
24	17325.0
25	19876.0
26	21673.0
27	23920.0
28	26844.0
29	32280.0
30	40558.0
31	51558.0
32	65435.0
33	84226.0
34	110070.0
35	144191.0
36	199734.0
37	309643.0
38	1002047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.10700141475777	19.700576230114663	13.629144774894572	26.563277580232995
2	12.843422114608554	20.504906333630686	42.04328618155559	24.608385370205173
3	17.370987722742676	25.7595621313988	29.623825583020018	27.2456245628385
4	11.145266437020581	17.12719030051025	37.879586976756826	33.84795628571234
5	12.123387097459194	39.54648888179169	35.38256506608749	12.947558954661629
6	31.088421702917934	37.434181148265715	18.499935631043087	12.977461517773268
7	28.025455240847375	31.91849223381425	21.891294647600027	18.16475787773835
8	25.043899657682516	38.02821389468246	20.252856929815355	16.67502951781967
9	27.158186144218575	14.750256322697322	20.14152750489073	37.95003002819337
10	15.159073577238363	29.650669028819536	33.940185700903015	21.250071693039082
11	33.954433850013544	22.40288996673328	25.149326554860362	18.49334962839281
12	23.785409930384972	25.01631569002524	31.803812585054956	19.394461794534827
13	29.223093764809192	22.34211594611534	25.916620098209904	22.51817019086556
14	20.50281922362755	23.04014171911709	27.836834486796846	28.62020457045851
15	22.665681421979876	32.343841902201724	23.237123340319226	21.753353335499174
16	21.262718763150843	28.352740492431273	28.844144113921388	21.540396630496495
17	20.672861749496597	29.491979994785535	28.346325625714975	21.488832630002896
18	20.60181351704599	27.40929654664027	31.334167309983684	20.654722626330052
19	23.06263959304646	26.86963389467784	28.275925029510923	21.791801482764768
20	23.74782277124385	27.203576302579386	28.803621821628173	20.244979104548595
21	22.669535335881847	26.728770213056862	27.530265182058166	23.07142926900313
22	23.06734315972191	28.73919307439598	27.519832600664007	20.6736311652181
23	21.433257285962316	28.286097767790984	28.737711301080925	21.542933645165768
24	22.844868033820912	26.867693284944956	29.328086871489283	20.95935180974485
25	21.891310292351772	27.753886445971904	29.09365515296963	21.261148108706692
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	109.0
2	338.5
3	568.0
4	568.0
5	568.0
6	1368.5
7	2169.0
8	2169.0
9	2169.0
10	2881.5
11	3594.0
12	3594.0
13	3594.0
14	5604.5
15	7615.0
16	7615.0
17	7615.0
18	15566.5
19	23518.0
20	23518.0
21	23518.0
22	41057.0
23	58596.0
24	58596.0
25	58596.0
26	89380.5
27	120165.0
28	120165.0
29	120165.0
30	157788.5
31	195412.0
32	195412.0
33	195412.0
34	220431.5
35	245451.0
36	245451.0
37	245451.0
38	260822.0
39	276193.0
40	276193.0
41	276193.0
42	289471.5
43	302750.0
44	302750.0
45	302750.0
46	312903.0
47	323056.0
48	323056.0
49	323056.0
50	304330.5
51	285605.0
52	285605.0
53	285605.0
54	245810.0
55	206015.0
56	206015.0
57	206015.0
58	173147.5
59	140280.0
60	140280.0
61	140280.0
62	113580.0
63	86880.0
64	86880.0
65	86880.0
66	65253.0
67	43626.0
68	43626.0
69	43626.0
70	32099.5
71	20573.0
72	20573.0
73	20573.0
74	14651.0
75	8729.0
76	8729.0
77	8729.0
78	5883.0
79	3037.0
80	3037.0
81	3037.0
82	1972.0
83	907.0
84	907.0
85	907.0
86	557.5
87	208.0
88	208.0
89	208.0
90	127.5
91	47.0
92	47.0
93	47.0
94	27.0
95	7.0
96	7.0
97	7.0
98	4.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08738441627592075
2	0.042927912951873606
3	0.01834308446608249
4	0.007770334391882166
5	0.02144272605410106
6	0.06335157875785896
7	0.08326571443978649
8	0.061483301636313534
9	0.02789677429216712
10	0.05630307870839209
11	0.008704472952654887
12	6.793734987437959E-4
13	0.0065814307690805224
14	0.0012738253101446174
15	0.015540668783764332
16	0.009511228982413142
17	0.005307605458935906
18	0.005477448833621855
19	0.0021655030272458497
20	0.002250424714588824
21	5.09530124057847E-4
22	0.001019060248115694
23	0.0
24	0.0
25	1.2738253101446175E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2355111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.45339619630982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.77086289342466	38.17078902896146
2	14.78327286451728	15.50865737281362
3	5.33810063143251	8.400045220689032
4	2.554124816821596	5.358900838062818
5	1.4094758202871573	3.6965896815320525
6	0.8404075289087114	2.644933744812621
7	0.532514984966391	1.9552553640839854
8	0.35229735536503254	1.4783354207899366
9	0.2441717379834965	1.1526873221150904
>10	1.0644297356272459	9.470867713364175
>50	0.059934198391225026	2.144564882290629
>100	0.042271415001570424	4.288456942105239
>500	0.004881610363881801	1.7821143395753574
>1k	0.0027662458728656605	2.385640788730179
>5k	4.8816103638805775E-4	1.5621613400738485
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8266	0.3509813337885136	No Hit
GTCCTACAGTGGACATTTCTAAATT	6749	0.28656823393886743	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5622	0.23871486312110132	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5560	0.2360822908134691	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5387	0.2287365648583018	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5021	0.21319589607453746	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4674	0.1984619833205314	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3540	0.15031138659706483	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2669	0.11332799175919947	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2592	0.11005850679649495	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2534	0.10759577786354868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.246084367148725E-5
6	0.0	0.0	0.0	0.0	4.246084367148725E-5
7	0.0	0.0	0.0	0.0	4.246084367148725E-5
8	0.0	0.0	0.0	0.0	4.246084367148725E-5
9	0.0	0.0	0.0	0.0	4.246084367148725E-5
10	0.0	0.0	0.0	0.0	4.246084367148725E-5
11	0.0	0.0	0.0	0.0	4.246084367148725E-5
12	0.0	0.0	0.0	0.0	4.246084367148725E-5
13	0.0	0.0	0.0	0.0	4.246084367148725E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCT	45	6.7062076E-4	14.794558	4
TCGCAAG	40	0.005311239	14.237403	18
TGTCGAG	155	0.0	14.084312	18
GTCGAGG	135	1.8189894E-12	13.357985	19
TAGGACC	1170	0.0	12.681049	4
TCGAATA	60	4.117422E-4	12.657889	11
GGTATCA	1910	0.0	12.350661	1
GGCGAGG	1360	0.0	11.864	19
CGAAATC	230	0.0	11.143972	13
TATACCG	60	0.0058277445	11.095918	5
GCCGGTT	180	3.6379788E-12	11.075652	11
TGTAGGA	3475	0.0	11.057615	2
CTAGGAC	465	0.0	11.045255	3
TCCAACG	225	0.0	10.968075	18
GTATCAA	4180	0.0	10.877359	1
CGGTAGG	70	0.0014762658	10.870857	1
CCAACGT	70	0.0015034752	10.847085	19
AGGACGT	3105	0.0	10.843215	5
GGACGTG	3080	0.0	10.838361	6
TGGCGAG	3145	0.0	10.774252	18
>>END_MODULE