FastQCFastQC Report
Thu 26 May 2016
SRR1512830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363029
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80430.3403682307749926No Hit
GTCCTAAAGTGTGTATTTCTCATTT77390.3275033865432883No Hit
CTGTAGGACGTGGAATATGGCAAGA66880.2830265730974948No Hit
CTTTAGGACGTGAAATATGGCGAGG61120.2586510787637393No Hit
GTATCAACGCAGAGTACTTTTTTTT57030.24134278504411075No Hit
GTCCTACAGTGTGCATTTCTCATTT49480.20939226729760826No Hit
TATCAACGCAGAGTACTTTTTTTTT36050.15255843241873038No Hit
GGTATCAACGCAGAGTACTTTTTTT33040.13982054388668103No Hit
CTGTAGGACCTGGAATATGGCGAGA32650.13817011979116633No Hit
CTGAAGGACCTGGAATATGGCGAGA30550.12928322081531796No Hit
GTCCTTCAGTGTGCATTTCTCATTT28240.11950763194188475No Hit
TTTCTAAATTTTCCACCTTTTTCAG26620.112652024160516No Hit
ATTTAGAAATGTCCACTGTAGGACG25430.10761611474086862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12700.013.0884344
GCGCCAC1750.013.02756913
TAGTACG751.4818133E-512.6646194
CCGTGCC550.003072487212.08921059
ACTGCGT658.035594E-411.6906648
ACCGAGT658.035594E-411.6906648
GGTATCA19500.011.6686511
TGTCGAG1800.011.61021718
CACCTTT10100.011.28626114
GGCGAGG16000.011.22029919
GTCGAGG1451.2205419E-911.13636619
GTCGTAC600.005783877311.1071881
TGTAGGA37550.011.0794332
ACCTTTT10900.010.98080615
CCACCTT10250.010.93574313
AGGACCT26100.010.844995
CTTTTTC13000.010.81455217
GGACCTG25300.010.7751666
GGGCGAA803.7763984E-410.68622511
GGACGTC803.7771376E-410.6859996
Produced by FastQC (version 0.11.5)