##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512830_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2363029
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.544005173021574	31.0	30.0	34.0	16.0	34.0
2	28.689111729056226	31.0	30.0	34.0	16.0	34.0
3	28.85512196422473	31.0	30.0	34.0	18.0	34.0
4	32.2548153238915	35.0	33.0	37.0	22.0	37.0
5	32.45111507306935	35.0	33.0	37.0	24.0	37.0
6	32.342991135529864	36.0	33.0	37.0	22.0	37.0
7	32.28338712728451	36.0	33.0	37.0	22.0	37.0
8	32.17749041590264	36.0	33.0	37.0	19.0	37.0
9	33.448116379443505	38.0	33.0	39.0	18.0	39.0
10	33.33123292181349	38.0	33.0	39.0	17.0	39.0
11	33.36235187972725	38.0	33.0	39.0	17.0	39.0
12	33.26097817673841	38.0	33.0	39.0	17.0	39.0
13	33.221716280248785	38.0	33.0	39.0	17.0	39.0
14	34.064299253204254	39.0	33.0	41.0	17.0	41.0
15	34.22471962891695	39.0	33.0	41.0	17.0	41.0
16	34.15711825796467	39.0	33.0	41.0	17.0	41.0
17	33.94700699822135	39.0	33.0	41.0	12.0	41.0
18	33.9255840702759	39.0	33.0	41.0	11.0	41.0
19	34.0332882076352	39.0	34.0	41.0	11.0	41.0
20	34.01421311376204	39.0	34.0	41.0	10.0	41.0
21	33.90850641274398	39.0	34.0	41.0	10.0	41.0
22	33.89695936867469	39.0	34.0	41.0	8.0	41.0
23	33.5608509248088	39.0	33.0	41.0	2.0	41.0
24	33.62056919318383	39.0	33.0	41.0	2.0	41.0
25	33.32622917450442	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7453698175344101
1101	2	0.4512830255075002
1101	3	0.7365024981685337
1101	4	0.8282737415804249
1101	5	0.7662880546778368
1101	6	0.6642129605578617
1101	7	0.48499109322197853
1101	8	0.692734259809157
1101	9	0.8598297932787986
1101	10	0.7868991620966455
1101	11	0.7632161105796342
1101	12	0.5288660729210335
1101	13	1.1170534911472316
1101	14	1.0325073759428918
1101	15	0.8516042483442163
1101	16	0.829890672366794
1101	17	0.1330338942732041
1101	18	0.6200347079430699
1101	19	0.11647948601571301
1101	20	-0.64196328501572
1101	21	0.6064994451062873
1101	22	0.6115121829027927
1101	23	0.2374884946519984
1101	24	0.744939842424337
1101	25	0.4238499238237594
1102	1	1.0391766623647385
1102	2	1.106645980101451
1102	3	1.0201435745395422
1102	4	1.173912925325297
1102	5	1.0692287833158076
1102	6	0.9685850437383294
1102	7	0.9432688333226054
1102	8	1.0138586785845405
1102	9	1.2678556880553629
1102	10	1.315270488987295
1102	11	1.0165709581487974
1102	12	1.2306637010125243
1102	13	1.1281493412377728
1102	14	1.276389720718413
1102	15	1.1933817766212087
1102	16	1.1136482211728236
1102	17	1.435119110803953
1102	18	1.4159228438774747
1102	19	1.1934942520039158
1102	20	1.4578345079708654
1102	21	1.487230177543367
1102	22	1.1917884487272516
1102	23	1.198582694433398
1102	24	0.5051639829290764
1102	25	0.8769786994152611
1103	1	0.7496442134813464
1103	2	0.884593941135261
1103	3	1.0618460463654316
1103	4	1.1128003286382082
1103	5	0.9312463808990685
1103	6	0.36217094809045847
1103	7	0.4444596482168848
1103	8	0.5339123093395415
1103	9	0.7246253174196582
1103	10	0.9379367931500298
1103	11	0.6955918632733926
1103	12	0.7849494107863677
1103	13	0.9213567483195959
1103	14	1.1006712904727394
1103	15	1.007588268055997
1103	16	1.0691657117682922
1103	17	0.6886439834253153
1103	18	0.9799689194258505
1103	19	0.9697108663522798
1103	20	1.2967510335443677
1103	21	1.1228166719732897
1103	22	1.049715507005203
1103	23	0.4840889546008569
1103	24	0.5332866380475565
1103	25	0.9729098340201148
1104	1	1.1998005108004257
1104	2	1.04292004561065
1104	3	1.0609287941184995
1104	4	1.2316560988067238
1104	5	0.8389375435560709
1104	6	0.8856270950150673
1104	7	1.0702943888249266
1104	8	0.8128139677849902
1104	9	1.1958548050005646
1104	10	1.1632443831942254
1104	11	0.9033802947053218
1104	12	1.2102380090380933
1104	13	1.2851257978573756
1104	14	1.5056702382247806
1104	15	1.1779964889356762
1104	16	1.2094116298161453
1104	17	1.3820145646254929
1104	18	1.222826208937768
1104	19	0.9187186735188604
1104	20	0.9566465273566322
1104	21	1.1603652421992905
1104	22	1.011505560438863
1104	23	1.3376653144388584
1104	24	1.1084353643142322
1104	25	0.681264169538359
1105	1	0.4317637621717836
1105	2	0.5952011713410847
1105	3	0.6346549311650556
1105	4	0.7493725436262437
1105	5	0.4933827978171976
1105	6	0.7711283928528587
1105	7	0.6415712566244878
1105	8	0.7168973618545706
1105	9	0.4352841399705767
1105	10	0.553109101186223
1105	11	0.5356203755165083
1105	12	0.8059858321229854
1105	13	0.7845056487658653
1105	14	0.9496317761945292
1105	15	0.935113498922
1105	16	0.6996861576717066
1105	17	1.1946419509208397
1105	18	0.9788557203625743
1105	19	0.6547486964068412
1105	20	0.8431464570304499
1105	21	0.7048228994422487
1105	22	0.7474371266669522
1105	23	0.9324400788461418
1105	24	0.9278051138251442
1105	25	1.0666817152823498
1106	1	0.6676365934016708
1106	2	0.7151948787799256
1106	3	0.4974965524991184
1106	4	0.6342186744055169
1106	5	0.5949478879222454
1106	6	0.7759606504802434
1106	7	0.6074896586091256
1106	8	0.767799083185345
1106	9	0.9265693452737693
1106	10	0.650054190204493
1106	11	0.46107371832222555
1106	12	0.7924106041518115
1106	13	0.7134635053773124
1106	14	0.9750073005466717
1106	15	0.7173841072275096
1106	16	0.833802103142439
1106	17	0.9901136548565077
1106	18	0.7851760546599138
1106	19	0.8327963119085453
1106	20	0.9576096874757098
1106	21	0.874709438795243
1106	22	0.7115238432581847
1106	23	0.9840239862582791
1106	24	0.6492844157936162
1106	25	0.7957340273960654
1107	1	0.9358792699568248
1107	2	0.8154553575584593
1107	3	0.753985542798258
1107	4	0.8447038055209788
1107	5	0.7049610727317699
1107	6	0.880593190779642
1107	7	0.6063553399494026
1107	8	0.7202994235138434
1107	9	0.6974927050072068
1107	10	0.8833015570093963
1107	11	0.739147726824747
1107	12	0.7821731150648574
1107	13	0.6071912034980116
1107	14	0.9179150158940104
1107	15	0.7102665866164983
1107	16	0.8488374838178672
1107	17	1.1521373898822986
1107	18	1.0070623384247313
1107	19	0.7721294759713544
1107	20	0.9464069090003164
1107	21	0.7364550749417447
1107	22	0.8038454731293427
1107	23	1.1444656625733458
1107	24	0.9360753736947984
1107	25	1.1329803872628688
1108	1	0.8073328210547928
1108	2	0.7484057339364405
1108	3	0.7128990457797251
1108	4	0.8297856916209625
1108	5	0.7151940464178992
1108	6	0.8437544226631317
1108	7	0.8121733251838847
1108	8	0.8620631727622765
1108	9	0.9196806200491139
1108	10	1.0252212911538194
1108	11	0.5853310963084013
1108	12	0.8880904416873605
1108	13	0.8653729160977903
1108	14	0.9232925983254603
1108	15	0.9292089222936042
1108	16	0.5891497816683255
1108	17	1.0010960587240447
1108	18	1.1275996353749136
1108	19	0.8991084086462706
1108	20	1.0352186880847256
1108	21	0.9875798850433313
1108	22	0.9995936969157597
1108	23	1.4518389906770182
1108	24	1.292742128555986
1108	25	1.4579764263651782
1109	1	0.6519886990754706
1109	2	0.6611502264794424
1109	3	0.8624891607051133
1109	4	0.8179449580342961
1109	5	0.6498909903226604
1109	6	0.8130971012167194
1109	7	0.6496085574333321
1109	8	0.5706403010766365
1109	9	0.6270696654888823
1109	10	0.6006698293687265
1109	11	0.5912398566082899
1109	12	0.4755611968409994
1109	13	0.6525084194467894
1109	14	0.8499480008316169
1109	15	0.5946127086834778
1109	16	0.26554526906367215
1109	17	0.8478361815981188
1109	18	0.8767938633602057
1109	19	0.777596863350702
1109	20	0.6406773978084388
1109	21	0.6366790101247517
1109	22	0.7357963899036122
1109	23	0.9084602489897264
1109	24	0.7038603051688312
1109	25	0.30040596791572227
1110	1	0.6618459685449665
1110	2	0.6602596721876424
1110	3	0.6652242720172872
1110	4	0.5656726931100451
1110	5	0.39776312038342354
1110	6	0.5342803434318384
1110	7	0.3984800715036627
1110	8	0.4615459777200712
1110	9	0.6749469169481301
1110	10	0.7074609460185286
1110	11	0.5163280229057889
1110	12	0.5026256539087441
1110	13	0.6201440262542661
1110	14	0.7162368220990771
1110	15	0.5829259618669269
1110	16	0.5965839313543881
1110	17	0.7258096157660958
1110	18	0.0583616656969852
1110	19	0.5736649096652187
1110	20	0.6610726559624851
1110	21	0.7611652698394025
1110	22	0.628119698099411
1110	23	0.859843239971795
1110	24	0.7131591999425879
1110	25	1.009028980350763
1111	1	0.2949701852144315
1111	2	0.2596654995175136
1111	3	0.31634176370367584
1111	4	0.2687371161117298
1111	5	0.24431922805935358
1111	6	0.150811416831246
1111	7	-0.052921678568729646
1111	8	0.18387058402695544
1111	9	0.25489183587068
1111	10	0.2095384343456601
1111	11	-0.049675301681993744
1111	12	-0.5239643904821563
1111	13	0.006610909150317923
1111	14	0.14608418994038885
1111	15	0.024706156871530993
1111	16	0.14760024308699826
1111	17	-0.2465528778158088
1111	18	0.11646291433896039
1111	19	0.04961675699864543
1111	20	0.2838871831335794
1111	21	0.15966669380107845
1111	22	-0.07985507395235203
1111	23	0.1699168517006271
1111	24	0.11813458524414244
1111	25	0.18662705697580861
1112	1	0.46368188569138624
1112	2	0.5455657865671739
1112	3	0.5499182344851512
1112	4	0.5479596309114072
1112	5	0.5504355805556145
1112	6	0.33914306278271766
1112	7	0.22064343869123348
1112	8	0.5666489798325856
1112	9	0.35766858112907585
1112	10	0.4632977192580867
1112	11	0.4351701441330178
1112	12	0.46107505472564725
1112	13	0.449732296950728
1112	14	0.423303413126888
1112	15	0.1688729112847227
1112	16	0.3551956181094269
1112	17	0.6659219141147545
1112	18	0.3521384912676524
1112	19	0.46181175045888523
1112	20	0.1715617016288249
1112	21	0.2359546931942731
1112	22	0.1393504853471299
1112	23	0.32400979153157294
1112	24	0.37948865760554895
1112	25	0.37144301060985185
1113	1	-0.0891625580877431
1113	2	0.04635423985010334
1113	3	0.08118681059832866
1113	4	0.22870224152889307
1113	5	0.001610630843266847
1113	6	-0.29159224587230526
1113	7	-0.3071891105118212
1113	8	-0.20840290825071506
1113	9	-0.28355837483280766
1113	10	-0.19275149617588028
1113	11	-0.25562185699997997
1113	12	-0.30682732565892934
1113	13	-0.17391331318324887
1113	14	0.07626821339079726
1113	15	-0.2519888886072934
1113	16	-0.414564227002046
1113	17	-0.25538700594465524
1113	18	-0.49150258937312685
1113	19	-0.752085736334628
1113	20	-0.4333739145157409
1113	21	-0.16065010466071072
1113	22	-0.6966096344174701
1113	23	-4.440366123843752
1113	24	-1.9935348370536694
1113	25	-1.0536568248424771
1114	1	-0.4535410413292098
1114	2	-0.6368348408761122
1114	3	-0.4604085092631536
1114	4	-0.2889379580650697
1114	5	-0.2716988301631176
1114	6	-1.0832865166197898
1114	7	-0.9092830399146941
1114	8	-0.6965052656202957
1114	9	-0.923075869879213
1114	10	-0.5958892874225867
1114	11	-0.8199147316431663
1114	12	-1.6326093630799967
1114	13	-0.8414558938495986
1114	14	-2.8238662387270423
1114	15	-1.5064089849532891
1114	16	-1.5576659766656178
1114	17	-1.400945083644146
1114	18	-1.310612347739223
1114	19	-1.394746501820201
1114	20	-0.9826893711511815
1114	21	-0.7488113709172239
1114	22	-0.8586351828929608
1114	23	-5.289005248335396
1114	24	-2.351401027605757
1114	25	-2.289317800868286
1115	1	-1.7456439393517194
1115	2	-1.6250054205656923
1115	3	-1.3820153311984065
1115	4	-1.3977656642478742
1115	5	-1.282431780514674
1115	6	-1.4307941573683145
1115	7	-2.1800289355540308
1115	8	-2.5215262540699257
1115	9	-3.6482939302572035
1115	10	-4.337212048090279
1115	11	-3.14845313582677
1115	12	-3.2858595865767803
1115	13	-2.5456855045959585
1115	14	-3.394661546979048
1115	15	-3.0171250900959805
1115	16	-2.991433848326224
1115	17	-2.7557655868837045
1115	18	-5.428261158144188
1115	19	-3.3840649378633643
1115	20	-3.871556423658209
1115	21	-3.493621770434533
1115	22	-2.9930883652932216
1115	23	-3.3929781312925016
1115	24	-3.3381159430342997
1115	25	-3.5345392915433536
1116	1	-0.8566858042700751
1116	2	-0.6459880872868453
1116	3	-1.1183145097796334
1116	4	-0.6970700562403565
1116	5	-0.6283235190384531
1116	6	-3.032153555811881
1116	7	-1.4282810124533079
1116	8	-1.9757136216183397
1116	9	-2.956591894516439
1116	10	-2.3984719655397377
1116	11	-1.6631172415194868
1116	12	-1.490201079609026
1116	13	-2.723412036947714
1116	14	-5.980871694741019
1116	15	-3.652371369775352
1116	16	-2.5241274246796372
1116	17	-3.436031978771098
1116	18	-2.2873069812851945
1116	19	-1.9179013196508699
1116	20	-2.7135100809275023
1116	21	-3.2533270054467067
1116	22	-2.9749485547975105
1116	23	-2.0216311679731973
1116	24	-2.853444422971716
1116	25	-2.8573122074924235
1201	1	0.6293090476984879
1201	2	0.6508776400002745
1201	3	0.9996262265727225
1201	4	0.8189120788648268
1201	5	0.4365000536340773
1201	6	0.8780814763377762
1201	7	1.0249446786750198
1201	8	0.9850101670876583
1201	9	1.4392235424278397
1201	10	1.2401931723794988
1201	11	1.451066912962311
1201	12	1.1940435438134998
1201	13	1.327346633757024
1201	14	1.5135127120351797
1201	15	1.4002039227380436
1201	16	1.354433424745551
1201	17	1.6821584983921696
1201	18	1.5116577945354024
1201	19	1.6775216309678669
1201	20	1.535721544737065
1201	21	1.744173288215805
1201	22	1.4753328504924994
1201	23	1.8587738820004986
1201	24	1.745615346286165
1201	25	1.7795965732719168
1202	1	0.9602562323280743
1202	2	0.9070897811639043
1202	3	0.9785214168230141
1202	4	0.98087793930614
1202	5	0.8358284178082513
1202	6	1.0262967908928502
1202	7	1.0954086346593286
1202	8	1.1018100093440637
1202	9	1.299416816455718
1202	10	1.2103150328442354
1202	11	1.1864106418851819
1202	12	1.3680884182157484
1202	13	1.2895516287982574
1202	14	1.7932496054205842
1202	15	1.4199947926468326
1202	16	1.2770333261830373
1202	17	1.530822219259008
1202	18	1.568184374344142
1202	19	1.535249684774989
1202	20	1.341614338194411
1202	21	1.4640933052429617
1202	22	1.5323634090919143
1202	23	1.542684131017225
1202	24	1.5866823460518091
1202	25	2.0276832309338744
1203	1	1.1166075259390773
1203	2	1.2090695233245086
1203	3	1.2346378430356957
1203	4	1.4226902306411304
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2211	18	-0.27441058033547705
2211	19	-0.03519969421434155
2211	20	-0.2890222253387762
2211	21	0.007812053874019398
2211	22	-0.0361860858474472
2211	23	0.18607666092107422
2211	24	-0.13512998732964832
2211	25	0.21180880825521342
2212	1	0.07474411802355263
2212	2	0.2068996565712773
2212	3	-0.2081768629567904
2212	4	0.11042875198406676
2212	5	-0.18671648456683698
2212	6	-0.25326699982187506
2212	7	-0.13650530591925047
2212	8	-0.004529322317253559
2212	9	-0.0023679292772129656
2212	10	-0.24732395352559422
2212	11	-0.32928410052006285
2212	12	0.19343147839722974
2212	13	0.11980995111629511
2212	14	0.22822734897304997
2212	15	-0.2148026961499312
2212	16	-0.07324433851491108
2212	17	0.1363976283206796
2212	18	0.2890810139529165
2212	19	0.03150800250886476
2212	20	0.19572898013569073
2212	21	0.270162956286228
2212	22	0.4161977900333085
2212	23	0.42042678748696005
2212	24	0.2525842682112298
2212	25	0.4609519160250173
2213	1	-0.08574585815436464
2213	2	0.030777367248948195
2213	3	-0.2183243407402493
2213	4	0.009411735226372286
2213	5	0.04678741139554177
2213	6	0.2622584730326878
2213	7	0.01651495525862856
2213	8	0.24908962631848652
2213	9	-0.11443487547626319
2213	10	0.1655480471076629
2213	11	0.05531979516327823
2213	12	-0.0643421990631694
2213	13	-0.04615237736465616
2213	14	-0.14529504247538227
2213	15	-0.051947841363876535
2213	16	0.14216433697536246
2213	17	0.09293933076599359
2213	18	-0.04571298401661039
2213	19	0.024678962099144997
2213	20	0.2401494277370233
2213	21	0.09825391930127836
2213	22	-0.08766902073190153
2213	23	0.07910774828295786
2213	24	0.3185729432873643
2213	25	0.42108492080426885
2214	1	-2.621375473407987
2214	2	-1.3213011181455592
2214	3	-1.4633662034650143
2214	4	-3.2932203110525364
2214	5	-1.7318267646593988
2214	6	-1.415806201246074
2214	7	-1.2653350911357109
2214	8	-1.0542126484945555
2214	9	-0.8122596374317581
2214	10	-0.7299370512083811
2214	11	-0.8480017604138368
2214	12	-0.7832581507876881
2214	13	-1.1921443885322738
2214	14	-1.1646081525704446
2214	15	-1.4560934276227755
2214	16	-1.249503455459596
2214	17	-3.4883427258826636
2214	18	-3.142901036792626
2214	19	-1.8680267569940767
2214	20	-1.6918887329541903
2214	21	-1.6882244618248876
2214	22	-2.8462729031420757
2214	23	-1.627135223593129
2214	24	-2.9088241662470367
2214	25	-2.8388121681174745
2215	1	-1.8850185998586113
2215	2	-3.6567087630853976
2215	3	-2.106090592846538
2215	4	-3.8453277631010003
2215	5	-2.51900929031115
2215	6	-2.1633549754581445
2215	7	-2.3366595652354043
2215	8	-1.5746664858426662
2215	9	-4.161538657781627
2215	10	-2.5181284932727017
2215	11	-1.9816870359054342
2215	12	-1.8626103718391
2215	13	-1.9927165862686884
2215	14	-1.7349634437804085
2215	15	-1.8713400730282217
2215	16	-1.9206677702051138
2215	17	-2.4167215631432555
2215	18	-2.0411538120129116
2215	19	-2.059343844509346
2215	20	-1.876930724461367
2215	21	-2.5895353180035343
2215	22	-2.3118696236386214
2215	23	-2.3631719534716886
2215	24	-2.2859906934671237
2215	25	-3.485727131377093
2216	1	-3.7417743762518505
2216	2	-3.7466776171025913
2216	3	-5.055418919214141
2216	4	-5.822932166083049
2216	5	-5.66931577022488
2216	6	-4.527294247554515
2216	7	-6.423129691282568
2216	8	-4.22879200673556
2216	9	-4.845358485710214
2216	10	-3.759227050081229
2216	11	-3.889973736337147
2216	12	-4.390616180509564
2216	13	-3.981474398893571
2216	14	-5.202225978716662
2216	15	-4.434794879383325
2216	16	-4.777025030489863
2216	17	-4.548275775966353
2216	18	-3.993083621042416
2216	19	-4.758467010701999
2216	20	-5.261808384565612
2216	21	-4.886574789665364
2216	22	-4.421767234140461
2216	23	-4.5478623442043755
2216	24	-4.9134734266900395
2216	25	-4.953474746858845
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	131455.0
3	3.0
4	1176.0
5	7104.0
6	8125.0
7	7414.0
8	6578.0
9	6138.0
10	5329.0
11	5178.0
12	4892.0
13	4878.0
14	4705.0
15	4846.0
16	5054.0
17	5480.0
18	6138.0
19	7508.0
20	9600.0
21	12303.0
22	15467.0
23	18647.0
24	21662.0
25	24257.0
26	27536.0
27	30094.0
28	33966.0
29	39910.0
30	47862.0
31	59460.0
32	73919.0
33	93086.0
34	118676.0
35	153533.0
36	207041.0
37	314770.0
38	839239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.662350984997865	18.98071955202511	12.761322932217812	24.59560653075921
2	14.280677905272032	19.927391476582102	43.27033766603127	22.521592952114602
3	19.08622094315068	25.872927533778423	28.945520722017076	26.095330801053823
4	11.882759090618602	16.43161292588898	38.637536170117315	33.0480918133751
5	12.432632151868948	39.315228625336154	35.56099737004047	12.69114185275443
6	31.144334188661478	36.94954043934701	18.77001300567447	13.13611236631704
7	28.33314275792264	31.605364909561377	22.20957366163124	17.851918670884736
8	25.1036030521546	37.76238587567295	20.677953465359536	16.456057606812916
9	27.95494317286663	14.518659187970842	20.269134794400802	37.257262844761726
10	15.97089039935485	28.744798831685447	34.20094298706316	21.083367781896545
11	34.15954825001091	21.977494664628658	25.63697282105298	18.225984264307456
12	24.55818803937233	24.469787525319315	31.83646879127228	19.135555644036078
13	29.552074836587188	22.45368664550792	25.63190380476351	22.36233471314138
14	20.62524194211008	22.957754472276083	27.72379168710204	28.693211898511795
15	22.564177060202958	32.97722351231152	22.91942572367939	21.53917370380613
16	21.260606204348957	27.92253500248611	29.348977899452716	21.467880893712216
17	21.038499498053636	29.44730833908701	28.25797078121492	21.256221381644437
18	20.835952550793053	27.01545606576876	31.624318650466204	20.52427273297198
19	23.660480756727402	26.427854088033264	27.855612873548807	22.056052281690523
20	24.25385777892233	27.041047987584626	28.685940877886136	20.019153355606907
21	23.009410230131373	26.554509033312723	27.039165530267738	23.396915206288167
22	23.596010598304357	29.075359365965546	27.095992595589124	20.232637440140973
23	21.630104400687195	28.538220952652033	28.50984199272821	21.321832653932564
24	23.327407930721495	26.5397893538431	29.34200924091738	20.790793474518015
25	22.266694451221163	27.642963392155266	28.92757549266759	21.162766663955978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	306.0
1	306.0
2	649.5
3	993.0
4	993.0
5	993.0
6	1785.0
7	2577.0
8	2577.0
9	2577.0
10	3047.0
11	3517.0
12	3517.0
13	3517.0
14	5579.0
15	7641.0
16	7641.0
17	7641.0
18	15379.5
19	23118.0
20	23118.0
21	23118.0
22	40003.5
23	56889.0
24	56889.0
25	56889.0
26	88140.0
27	119391.0
28	119391.0
29	119391.0
30	158018.5
31	196646.0
32	196646.0
33	196646.0
34	221043.5
35	245441.0
36	245441.0
37	245441.0
38	260599.5
39	275758.0
40	275758.0
41	275758.0
42	286978.5
43	298199.0
44	298199.0
45	298199.0
46	307655.5
47	317112.0
48	317112.0
49	317112.0
50	300354.5
51	283597.0
52	283597.0
53	283597.0
54	245085.0
55	206573.0
56	206573.0
57	206573.0
58	175674.0
59	144775.0
60	144775.0
61	144775.0
62	118837.5
63	92900.0
64	92900.0
65	92900.0
66	71193.0
67	49486.0
68	49486.0
69	49486.0
70	36375.0
71	23264.0
72	23264.0
73	23264.0
74	16436.0
75	9608.0
76	9608.0
77	9608.0
78	6552.0
79	3496.0
80	3496.0
81	3496.0
82	2373.5
83	1251.0
84	1251.0
85	1251.0
86	793.0
87	335.0
88	335.0
89	335.0
90	222.5
91	110.0
92	110.0
93	110.0
94	71.0
95	32.0
96	32.0
97	32.0
98	23.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33418972005845043
2	0.00935240320791662
3	0.008632987576538418
4	0.009987181706191502
5	0.010791234470673022
6	0.07414212859850641
7	0.07443835856436802
8	0.09885617146467521
9	0.07756993248919078
10	0.08552582300090265
11	0.08040527644815193
12	0.0923814307822714
13	0.07748529535608746
14	0.08154787774504672
15	0.07896644518539553
16	0.08087078068022018
17	0.09390489917813112
18	0.0961901017719207
19	0.08996927248882684
20	0.08823421126020883
21	0.07486154422988461
22	0.09623242033847236
23	0.08992695392227518
24	0.08497568163573109
25	0.08484872593607612
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2363029.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.16297561273469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56831449018848	43.003043794206555
2	13.387452440855018	15.037583299047718
3	4.5019655025952545	7.585313361948905
4	2.0278161993748953	4.5555276701040235
5	1.0481538339053635	2.943371910601065
6	0.6153447065615835	2.0735753848826155
7	0.39807119638692834	1.5649804026367824
8	0.2773258517330075	1.2460356038129419
9	0.19657093617914287	0.9936007825321465
>10	0.8773245017199292	8.62921619161474
>50	0.05636452362226198	2.19018446609013
>100	0.03780325096228724	4.247407412674342
>500	0.004162536619898196	1.5865190950317145
>1k	0.002951616875927211	2.886338356278943
>5k	3.784124199906682E-4	1.4573022685372734
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	8043	0.3403682307749926	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7739	0.3275033865432883	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6688	0.2830265730974948	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6112	0.2586510787637393	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5703	0.24134278504411075	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4948	0.20939226729760826	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3605	0.15255843241873038	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3304	0.13982054388668103	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3265	0.13817011979116633	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3055	0.12928322081531796	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2824	0.11950763194188475	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2662	0.112652024160516	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2543	0.10761611474086862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1270	0.0	13.088434	4
GCGCCAC	175	0.0	13.027569	13
TAGTACG	75	1.4818133E-5	12.664619	4
CCGTGCC	55	0.0030724872	12.0892105	9
ACTGCGT	65	8.035594E-4	11.690664	8
ACCGAGT	65	8.035594E-4	11.690664	8
GGTATCA	1950	0.0	11.668651	1
TGTCGAG	180	0.0	11.610217	18
CACCTTT	1010	0.0	11.286261	14
GGCGAGG	1600	0.0	11.220299	19
GTCGAGG	145	1.2205419E-9	11.136366	19
GTCGTAC	60	0.0057838773	11.107188	1
TGTAGGA	3755	0.0	11.079433	2
ACCTTTT	1090	0.0	10.980806	15
CCACCTT	1025	0.0	10.935743	13
AGGACCT	2610	0.0	10.84499	5
CTTTTTC	1300	0.0	10.814552	17
GGACCTG	2530	0.0	10.775166	6
GGGCGAA	80	3.7763984E-4	10.686225	11
GGACGTC	80	3.7771376E-4	10.685999	6
>>END_MODULE