FastQCFastQC Report
Thu 26 May 2016
SRR1512830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363029
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74880.3168814263388219No Hit
GTCCTAAAGTGTGTATTTCTCATTT69450.29390244470127114No Hit
CTGTAGGACGTGGAATATGGCAAGA68780.29106710074230996No Hit
GTATCAACGCAGAGTACTTTTTTTT68480.28979754374576017No Hit
CTTTAGGACGTGAAATATGGCGAGG67140.2841268558278379No Hit
TATCAACGCAGAGTACTTTTTTTTT51170.21654410504483865No Hit
GTCCTACAGTGTGCATTTCTCATTT45810.19386135337314947No Hit
GGTATCAACGCAGAGTACTTTTTTT39980.16918962907353233No Hit
CTGAAGGACCTGGAATATGGCGAGA32990.13960895105392274No Hit
CTGTAGGACCTGGAATATGGCGAGA32980.13956663248737108No Hit
TTTCTAAATTTTCCACCTTTTTCAG28510.12065023323877956No Hit
ATTTAGAAATGTCCACTGTAGGACG27120.11476795248809896No Hit
GTCCTTCAGTGTGCATTTCTCATTT24470.10355353235190934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12800.013.6705584
GGTATCA18050.012.6983081
CACCTTT9550.012.42571714
ACCGTCT550.003068078312.0915298
GGCGAGG17000.012.00413619
TCCAACG1406.91216E-1011.52605318
TATGTCG2150.011.47996416
CCACCTT10400.011.22759213
ATGTCGA2200.011.21810717
ACGCTGT600.00583247611.0947116
TGTCGAG2400.011.0740518
GACGGGA951.3505918E-511.0075587
AGGACCT24550.010.9625955
GTCGAGG2000.010.91538819
TTCCACC10400.010.7718711
TGTAGGA39950.010.7125432
ACCTTTT10850.010.67443615
TTAGGAC26000.010.6442843
GACGTGA22700.010.63725957
CTTTTTC13150.010.61110217
Produced by FastQC (version 0.11.5)