##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512830_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2363029 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.541107197584118 31.0 31.0 34.0 27.0 34.0 2 30.542297195675552 31.0 31.0 34.0 27.0 34.0 3 30.726133703818277 31.0 31.0 34.0 27.0 34.0 4 34.33760906023583 37.0 35.0 37.0 31.0 37.0 5 33.991147802248726 37.0 35.0 37.0 30.0 37.0 6 33.89971261461455 37.0 35.0 37.0 30.0 37.0 7 33.74546186271942 37.0 35.0 37.0 29.0 37.0 8 33.69415821811751 37.0 35.0 37.0 28.0 37.0 9 35.071604284162404 39.0 35.0 39.0 28.0 39.0 10 34.974287238963214 39.0 35.0 39.0 28.0 39.0 11 34.984873651571775 39.0 35.0 39.0 27.0 39.0 12 34.876434863897146 39.0 35.0 39.0 27.0 39.0 13 34.871932168416045 39.0 35.0 39.0 27.0 39.0 14 35.981728535705656 40.0 36.0 41.0 27.0 41.0 15 35.997580647550244 40.0 36.0 41.0 27.0 41.0 16 35.99539489358785 40.0 36.0 41.0 27.0 41.0 17 35.98726422739628 40.0 36.0 41.0 27.0 41.0 18 35.91260454272884 40.0 36.0 41.0 27.0 41.0 19 35.92008181025286 40.0 36.0 41.0 27.0 41.0 20 35.90621824785053 40.0 36.0 41.0 27.0 41.0 21 35.87628420980022 40.0 36.0 41.0 27.0 41.0 22 35.83859571761498 40.0 36.0 41.0 27.0 41.0 23 35.69118872430258 40.0 36.0 41.0 27.0 41.0 24 35.66743954475379 40.0 36.0 41.0 27.0 41.0 25 35.607040370642935 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.603353807549972 1101 2 0.5000443542852331 1101 3 0.4118835100715579 1101 4 0.4837795475534463 1101 5 0.5165669763082406 1101 6 0.6010956535452934 1101 7 0.6048544814868961 1101 8 0.37901952942329586 1101 9 0.7068843641078715 1101 10 0.4020388002920825 1101 11 0.7496345882651383 1101 12 0.8560040306178465 1101 13 0.9304688272018495 1101 14 1.072372393860121 1101 15 0.828768897895948 1101 16 1.049572383618873 1101 17 1.1138440183855849 1101 18 0.9023059109636051 1101 19 0.7365736046811335 1101 20 0.8539026765952542 1101 21 0.9601971764145745 1101 22 0.9665661859552728 1101 23 1.0751146785757015 1101 24 1.101413143501226 1101 25 1.0614916623368131 1102 1 0.7170804133992412 1102 2 0.7761246378178477 1102 3 0.5417130582658132 1102 4 0.5981547316851632 1102 5 0.7759838915286679 1102 6 0.6914176838683304 1102 7 0.7630802130798031 1102 8 0.8245468996305618 1102 9 1.017006993622168 1102 10 0.8980167999734263 1102 11 1.0810604675844857 1102 12 0.972580509814378 1102 13 1.0208559190950055 1102 14 1.1044171866458115 1102 15 1.1530466974892022 1102 16 1.0794577163368118 1102 17 1.149703198525586 1102 18 0.46332748356016396 1102 19 1.0239371129096568 1102 20 1.0967447833024835 1102 21 0.9853643228480635 1102 22 0.822223561132482 1102 23 1.1153230005617445 1102 24 1.1162898314516738 1102 25 1.1023874093882995 1103 1 0.8356589417789024 1103 2 0.9357077828588132 1103 3 0.6958466032410833 1103 4 0.6048965415922041 1103 5 0.7263289896587111 1103 6 0.6488157513503836 1103 7 0.8206477371138945 1103 8 0.9349767878868818 1103 9 0.9859149338532305 1103 10 0.8851310221379833 1103 11 1.036468996445791 1103 12 1.2109283156698325 1103 13 1.1171320333932329 1103 14 1.254069153918337 1103 15 1.305221826368033 1103 16 1.2672416188987015 1103 17 1.2725876235510967 1103 18 1.2534078336273708 1103 19 1.192919655081667 1103 20 1.0310050634078323 1103 21 1.0916067511261076 1103 22 1.0691173621309815 1103 23 1.2302574958728414 1103 24 1.2414805473855353 1103 25 1.1403206342687326 1104 1 0.6857236165316252 1104 2 0.7754558079690419 1104 3 0.6490074534574362 1104 4 0.5761568266744064 1104 5 0.6973018313954853 1104 6 0.6769877656609538 1104 7 0.5811635439314315 1104 8 0.6237924186453725 1104 9 0.6928081845808265 1104 10 0.7880483154482505 1104 11 0.7687245027823764 1104 12 0.8211995389979165 1104 13 0.7448954675199388 1104 14 1.0378175691302474 1104 15 0.9934836153948794 1104 16 0.9640938576203979 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16 0.7672297015518126 1107 17 0.7449553747770565 1107 18 0.7966034364195167 1107 19 0.8169613920521783 1107 20 0.9079321366374415 1107 21 0.7965855000111688 1107 22 0.8364511750472445 1107 23 0.9513351146365281 1107 24 0.9114205119980241 1107 25 1.0021546744430694 1108 1 0.4591879540844097 1108 2 0.3935184834719685 1108 3 0.37725521454947497 1108 4 0.23887915146886485 1108 5 0.561713476627439 1108 6 0.453481035333418 1108 7 0.5072187760223841 1108 8 0.3304641555722654 1108 9 0.6270866026090189 1108 10 0.5704889117817231 1108 11 0.5231526495600818 1108 12 0.5560844900690611 1108 13 0.6490643856436762 1108 14 0.8429381734107793 1108 15 0.6713587629786844 1108 16 0.6069071433894635 1108 17 0.7437556988385055 1108 18 0.7720004955951723 1108 19 0.7279786077695505 1108 20 0.8153884723360463 1108 21 0.746195214111971 1108 22 0.7994891021902291 1108 23 0.8094807408030817 1108 24 0.7991132722814811 1108 25 0.8604849434881032 1109 1 0.23388481357831026 1109 2 0.17765458754493224 1109 3 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-2.8694577010784954 2216 18 -3.2680455361406118 2216 19 -3.5826393676683708 2216 20 -2.7924124678748683 2216 21 -3.338207712396219 2216 22 -2.7782257857739623 2216 23 -4.333980804320515 2216 24 -3.5142643319548483 2216 25 -3.576422152698772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51887.0 3 2.0 4 784.0 5 7345.0 6 8963.0 7 7021.0 8 5742.0 9 4939.0 10 4155.0 11 3786.0 12 3564.0 13 3274.0 14 3254.0 15 3244.0 16 3390.0 17 3695.0 18 4129.0 19 4961.0 20 6510.0 21 8676.0 22 11015.0 23 13683.0 24 16387.0 25 19046.0 26 21370.0 27 23664.0 28 26758.0 29 32044.0 30 40704.0 31 52348.0 32 66753.0 33 86507.0 34 112544.0 35 147023.0 36 203010.0 37 315328.0 38 1035524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.92257583586755 19.956459496319386 13.62051147385452 26.500453193958545 2 13.014186664137744 20.479871566849717 42.11600558338735 24.389936185625192 3 17.297752825634152 25.94990956870218 29.800763322267688 26.951574283395985 4 11.35292340168725 17.19782233511561 38.01632571528691 33.43292854791024 5 12.135249407523007 39.573230325509975 35.55086286406277 12.74065740290425 6 31.198268021401603 37.59190444816682 18.301497401015883 12.90833012941569 7 28.08323782853861 31.788485044076204 22.248805448897947 17.87947167848724 8 25.0862534742747 38.20732506677552 20.232177246020143 16.474244212929637 9 27.247386966069925 14.545917097445466 20.189909532219094 38.01678640426551 10 15.545343425221336 29.2982293470037 34.03734371143196 21.119083516343007 11 33.976502433679535 21.982975881581947 25.68967655190714 18.350845132831374 12 24.26594713270484 24.713215469585204 31.783093688518587 19.237743709191367 13 29.38029539972068 22.395276990139234 25.786872063989165 22.437555546150918 14 20.4754365941362 23.007930150016485 27.68100136817199 28.835631887675323 15 22.379795117144845 33.027583509825135 22.911429050296352 21.681192322733665 16 21.254874476155166 28.05979978144741 29.085820405972868 21.599505336424556 17 20.831587616370552 29.669866936163046 28.28941744261141 21.209128004854996 18 20.827701144268964 27.079706090720496 31.597956076119825 20.494636688890715 19 23.584578684031115 26.51853224877475 27.944014974255065 21.95287409293907 20 24.206656526902115 27.133688309731717 28.54170210929109 20.117953054075077 21 22.698509493982918 26.508544351874697 27.324067518598 23.468878635544385 22 23.49309714859317 28.84699127256815 27.291305280682383 20.36860629815629 23 21.390596560600823 28.53841404400877 28.62622506960346 21.444764325786945 24 23.311351659247517 26.692393533892307 29.18296813115709 20.81328667570309 25 22.05405272730812 27.824789062837223 28.8805116829015 21.240646526953153 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 82.0 1 82.0 2 298.0 3 514.0 4 514.0 5 514.0 6 1252.0 7 1990.0 8 1990.0 9 1990.0 10 2532.0 11 3074.0 12 3074.0 13 3074.0 14 5234.5 15 7395.0 16 7395.0 17 7395.0 18 15448.0 19 23501.0 20 23501.0 21 23501.0 22 41097.5 23 58694.0 24 58694.0 25 58694.0 26 90835.5 27 122977.0 28 122977.0 29 122977.0 30 160936.5 31 198896.0 32 198896.0 33 198896.0 34 222900.5 35 246905.0 36 246905.0 37 246905.0 38 261946.0 39 276987.0 40 276987.0 41 276987.0 42 288692.5 43 300398.0 44 300398.0 45 300398.0 46 309785.0 47 319172.0 48 319172.0 49 319172.0 50 301860.0 51 284548.0 52 284548.0 53 284548.0 54 244347.0 55 204146.0 56 204146.0 57 204146.0 58 172972.5 59 141799.0 60 141799.0 61 141799.0 62 115486.0 63 89173.0 64 89173.0 65 89173.0 66 68136.0 67 47099.0 68 47099.0 69 47099.0 70 34549.5 71 22000.0 72 22000.0 73 22000.0 74 15491.5 75 8983.0 76 8983.0 77 8983.0 78 6095.5 79 3208.0 80 3208.0 81 3208.0 82 2166.0 83 1124.0 84 1124.0 85 1124.0 86 715.0 87 306.0 88 306.0 89 306.0 90 178.5 91 51.0 92 51.0 93 51.0 94 29.0 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08501800020228276 2 0.043249575015795405 3 0.019551177746866415 4 0.005543732218267317 5 0.020736097610312865 6 0.05657992347956796 7 0.08002440934918699 8 0.05861121467404758 9 0.02712620115961336 10 0.047904617336477884 11 0.0061785107165422005 12 7.617341979298603E-4 13 0.005459095085163999 14 9.31008464136496E-4 15 0.013245711330669239 16 0.00892921754240003 17 0.004358812354820867 18 0.005205183685854046 19 0.0016927426620663563 20 0.00232752116034124 21 2.539113993099535E-4 22 8.463713310331781E-4 23 0.0 24 0.0 25 1.2695569965497674E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2363029.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.60838628522696 #Duplication Level Percentage of deduplicated Percentage of total 1 74.20025202992547 39.7775577328144 2 14.567064719086295 15.618336650053585 3 5.048068764550495 8.118564609732342 4 2.2418131173829092 4.807199343038074 5 1.1759164903491126 3.15194927269018 6 0.671441402299913 2.1596934037432933 7 0.4775310859078449 1.7919769641586158 8 0.3035429075942403 1.301795635556239 9 0.21905570860714235 1.0568900740496223 >10 0.9829829226781409 9.198913886528981 >50 0.06312774496036354 2.3541116032628944 >100 0.04091700061889731 4.355556874118552 >500 0.005129494214732162 1.9142687418343887 >1k 0.002683120050782218 2.7013996002079423 >5k 4.734917736674502E-4 1.6917856082110345 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7488 0.3168814263388219 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6945 0.29390244470127114 No Hit CTGTAGGACGTGGAATATGGCAAGA 6878 0.29106710074230996 No Hit GTATCAACGCAGAGTACTTTTTTTT 6848 0.28979754374576017 No Hit CTTTAGGACGTGAAATATGGCGAGG 6714 0.2841268558278379 No Hit TATCAACGCAGAGTACTTTTTTTTT 5117 0.21654410504483865 No Hit GTCCTACAGTGTGCATTTCTCATTT 4581 0.19386135337314947 No Hit GGTATCAACGCAGAGTACTTTTTTT 3998 0.16918962907353233 No Hit CTGAAGGACCTGGAATATGGCGAGA 3299 0.13960895105392274 No Hit CTGTAGGACCTGGAATATGGCGAGA 3298 0.13956663248737108 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2851 0.12065023323877956 No Hit ATTTAGAAATGTCCACTGTAGGACG 2712 0.11476795248809896 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2447 0.10355353235190934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 4.231856655165891E-5 0.0 4.231856655165891E-5 0.0 11 0.0 4.231856655165891E-5 0.0 4.231856655165891E-5 0.0 12 0.0 4.231856655165891E-5 0.0 4.231856655165891E-5 0.0 13 0.0 4.231856655165891E-5 0.0 4.231856655165891E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1280 0.0 13.670558 4 GGTATCA 1805 0.0 12.698308 1 CACCTTT 955 0.0 12.425717 14 ACCGTCT 55 0.0030680783 12.091529 8 GGCGAGG 1700 0.0 12.004136 19 TCCAACG 140 6.91216E-10 11.526053 18 TATGTCG 215 0.0 11.479964 16 CCACCTT 1040 0.0 11.227592 13 ATGTCGA 220 0.0 11.218107 17 ACGCTGT 60 0.005832476 11.094711 6 TGTCGAG 240 0.0 11.07405 18 GACGGGA 95 1.3505918E-5 11.007558 7 AGGACCT 2455 0.0 10.962595 5 GTCGAGG 200 0.0 10.915388 19 TTCCACC 1040 0.0 10.77187 11 TGTAGGA 3995 0.0 10.712543 2 ACCTTTT 1085 0.0 10.674436 15 TTAGGAC 2600 0.0 10.644284 3 GACGTGA 2270 0.0 10.6372595 7 CTTTTTC 1315 0.0 10.611102 17 >>END_MODULE