FastQCFastQC Report
Thu 26 May 2016
SRR1512829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972097
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69240.3510983486106413No Hit
GTCCTACAGTGGACATTTCTAAATT41240.2091175028408846No Hit
GGTATCAACGCAGAGTACTTTTTTT40540.20556798169664067No Hit
TATCAACGCAGAGTACTTTTTTTTT39620.2009028967642058No Hit
CTGTAGGACGTGGAATATGGCAAGA38290.19415880659014237No Hit
GTACTGGTTCACTATCGGTCAGTCA37010.18766825364066778No Hit
GTCCTAAAGTGTGTATTTCTCATTT34340.17412936584762312No Hit
CTTTAGGACGTGAAATATGGCGAGG27540.13964830330353933No Hit
GGATACCACGTGTCCCGCCCTACTC21930.11120142670467022No Hit
GTCCTACAGTGTGCATTTCTCATTT20680.10486299608994892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG707.272023E-613.5701425
TAGGACC6100.013.08105854
GGTATCA20450.012.6178321
GGCGTGT853.9490114E-612.2929528
TATGCGA550.003072880212.08884112
GTCTTAT1209.687938E-911.9019671
CCGTATG802.872294E-511.872979
AGACCCG1251.8349965E-811.3989195
AGATCGT600.005880756311.0822826
ATAGGCG600.005880756311.0822825
CGAGACG600.005881867411.08200118
GTCCTAC21450.010.9198471
GTATGCG700.001493837910.85528711
AGGACCT12300.010.8119835
TGTAGGA20350.010.6896752
CTACACG803.7740608E-410.6867584
GTCCTAT1252.171746E-710.6641641
CTTAGAC1252.2428321E-710.6392613
GGACCTG12500.010.5629996
CTAATAC1355.69471E-810.5548223
Produced by FastQC (version 0.11.5)