##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512829_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1972097
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.77143619203315	31.0	30.0	34.0	22.0	34.0
2	28.89811048848003	31.0	30.0	34.0	18.0	34.0
3	29.052808761435163	31.0	30.0	34.0	22.0	34.0
4	32.45057317160363	35.0	33.0	37.0	23.0	37.0
5	32.65443991852328	35.0	33.0	37.0	25.0	37.0
6	32.53351990292567	36.0	33.0	37.0	23.0	37.0
7	32.478740650181	37.0	33.0	37.0	23.0	37.0
8	32.354994201603674	36.0	33.0	37.0	22.0	37.0
9	33.65374370530456	38.0	34.0	39.0	19.0	39.0
10	33.533866741848904	38.0	34.0	39.0	18.0	39.0
11	33.573432746969345	38.0	34.0	39.0	19.0	39.0
12	33.47454004544401	38.0	34.0	39.0	17.0	39.0
13	33.43373424329533	38.0	34.0	39.0	17.0	39.0
14	34.28033712337679	39.0	34.0	41.0	17.0	41.0
15	34.45518146419776	39.0	34.0	41.0	17.0	41.0
16	34.397654882087444	39.0	34.0	41.0	17.0	41.0
17	34.15900637747535	39.0	33.0	41.0	17.0	41.0
18	34.16549185968033	39.0	33.0	41.0	17.0	41.0
19	34.27940664176255	39.0	34.0	41.0	18.0	41.0
20	34.25163569540444	39.0	34.0	41.0	16.0	41.0
21	34.139613822240996	39.0	34.0	41.0	11.0	41.0
22	34.152527994312656	39.0	34.0	41.0	10.0	41.0
23	33.811208576454405	39.0	34.0	41.0	2.0	41.0
24	33.87746190983506	39.0	34.0	41.0	2.0	41.0
25	33.58802178594664	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5189204150020146
1101	2	0.2690854527716553
1101	3	0.6148774618364179
1101	4	0.6552134571225849
1101	5	0.5988869520026014
1101	6	0.5411476725092967
1101	7	0.23766218786940385
1101	8	0.5230057790055014
1101	9	0.6222849648917901
1101	10	0.5966421848837555
1101	11	0.6559866590507752
1101	12	0.400585980159498
1101	13	0.8725651328781296
1101	14	0.9268449998024764
1101	15	0.7185465919217435
1101	16	0.6364929842813467
1101	17	0.23550530873391295
1101	18	0.6596173888015215
1101	19	0.06671694711803866
1101	20	-0.6084862400134625
1101	21	0.6364454078225208
1101	22	0.55040063302976
1101	23	0.3447286029806378
1101	24	0.7310634277182047
1101	25	0.4392828196495273
1102	1	1.045421677136897
1102	2	0.9910904263328746
1102	3	1.0292665135750205
1102	4	1.218539334279555
1102	5	1.1117921472853993
1102	6	0.9575812220895941
1102	7	0.9543450403299403
1102	8	0.8881193532929359
1102	9	1.2286117953444062
1102	10	1.2150802438738282
1102	11	1.1198020374591664
1102	12	1.2131071793840036
1102	13	1.1185189819432182
1102	14	1.3015517810550463
1102	15	1.3205911790233316
1102	16	1.2924929450069982
1102	17	1.516311575492459
1102	18	1.488446370417556
1102	19	1.207914047242646
1102	20	1.4590821598422963
1102	21	1.5531775317438132
1102	22	1.3717790914042993
1102	23	1.3070426299942497
1102	24	0.7872511912580364
1102	25	1.1678345600389122
1103	1	0.6473396700324514
1103	2	0.7493873122557844
1103	3	0.8537308573190678
1103	4	0.9848289347429571
1103	5	0.673348763460119
1103	6	0.4609699908279694
1103	7	0.31586226399406314
1103	8	0.5277322938619662
1103	9	0.6634144966190192
1103	10	0.8719475566236454
1103	11	0.8228966122398589
1103	12	0.8802376634119398
1103	13	0.990452593926129
1103	14	1.1094807521347363
1103	15	0.954485632954345
1103	16	0.9530052502101682
1103	17	0.7591021857660607
1103	18	1.0068809017027078
1103	19	0.9978604880818978
1103	20	1.041013733241229
1103	21	0.9888923161754164
1103	22	0.9950181729123173
1103	23	0.40747165925763085
1103	24	0.48002290260834
1103	25	0.8369286357328747
1104	1	0.9808626630896455
1104	2	0.9517847511356621
1104	3	0.9651967114302913
1104	4	1.0837440741239561
1104	5	0.8910668588453774
1104	6	0.7564337361470166
1104	7	1.0513260009148908
1104	8	0.7371843396255287
1104	9	1.0315823374963315
1104	10	1.0126898036000753
1104	11	0.8573674070218757
1104	12	1.1369715634009339
1104	13	1.126176801533397
1104	14	1.3610948893565507
1104	15	0.9868893117091275
1104	16	1.112979308119229
1104	17	1.3210448066310931
1104	18	1.0695478687455875
1104	19	0.6974549172089297
1104	20	0.7874484803583357
1104	21	0.9310899110941548
1104	22	0.7496355123741054
1104	23	1.2148528937786693
1104	24	0.9072420683463065
1104	25	0.5613364354925139
1105	1	0.6286514262429748
1105	2	0.8135163547357962
1105	3	0.9032774936451062
1105	4	0.9784828876203875
1105	5	0.7703394860582122
1105	6	0.9815838316453522
1105	7	0.9311665962999243
1105	8	0.8703556522732256
1105	9	0.6421768634203318
1105	10	0.713043844702959
1105	11	0.7618940739264985
1105	12	0.926819189389434
1105	13	0.8189105856996832
1105	14	1.0296934501994954
1105	15	1.044593383897734
1105	16	1.034432650893109
1105	17	1.3237318127976039
1105	18	1.015812414499706
1105	19	0.8255049706232569
1105	20	0.9487775881357408
1105	21	0.8668848133671077
1105	22	0.8864012936717103
1105	23	1.0761613745789802
1105	24	1.0741286315930978
1105	25	1.2401325878349851
1106	1	0.7353935537555536
1106	2	0.7514441934406868
1106	3	0.6605842799736656
1106	4	0.7759589184428535
1106	5	0.664807675980633
1106	6	0.7853320294635537
1106	7	0.6971942621570193
1106	8	0.7395734085871908
1106	9	0.7801737901925136
1106	10	0.5810413925870748
1106	11	0.37944142201251196
1106	12	0.6048243395134634
1106	13	0.6757625777035088
1106	14	0.8771989838388734
1106	15	0.6074409779185572
1106	16	0.7319585833799422
1106	17	0.8839882852306573
1106	18	0.7985153404899705
1106	19	0.8202567874820232
1106	20	0.8461519225143306
1106	21	0.9863801556657279
1106	22	1.0367640911088145
1106	23	1.217617490680965
1106	24	0.7992144918097281
1106	25	1.0367340632736273
1107	1	1.0239470593242537
1107	2	0.9571020203938332
1107	3	0.8745848999845158
1107	4	0.9992015751893462
1107	5	0.9111320601867661
1107	6	1.0523880264729115
1107	7	0.8049792733947214
1107	8	1.0997529837885764
1107	9	1.122895045167411
1107	10	1.3122438945132089
1107	11	1.1510996319135387
1107	12	1.1505273451984124
1107	13	0.9841495921233161
1107	14	1.3192256898944663
1107	15	1.145754001337643
1107	16	1.2263556912116798
1107	17	1.484082426584017
1107	18	1.4621252624871346
1107	19	1.2715084343858436
1107	20	1.4383318077211413
1107	21	1.2191782737504369
1107	22	1.2134341313639254
1107	23	1.4985553482699387
1107	24	1.4045935418254558
1107	25	1.6447059895470417
1108	1	0.9170722380313912
1108	2	0.8176780429565653
1108	3	0.8600074680463337
1108	4	0.919156685149666
1108	5	0.854055038852465
1108	6	0.9423150682280408
1108	7	0.8612912591705282
1108	8	0.8402302546268743
1108	9	0.9340141783537916
1108	10	0.9855971741147656
1108	11	0.7211770879113217
1108	12	0.9373040311697594
1108	13	0.9772804775284456
1108	14	1.0404714879155321
1108	15	0.9975531422708102
1108	16	0.736925112297989
1108	17	1.0677635967474117
1108	18	1.156648005567611
1108	19	0.9463446601070231
1108	20	1.0222128117341285
1108	21	0.9076985448563732
1108	22	0.9300967748416511
1108	23	1.317685532168781
1108	24	1.1749184338853524
1108	25	1.2839228754874483
1109	1	0.6738616549107554
1109	2	0.6355391315270715
1109	3	0.7256176666392697
1109	4	0.6575068668119002
1109	5	0.6475519452537668
1109	6	0.7109862635639743
1109	7	0.6400822128853179
1109	8	0.584370272450343
1109	9	0.5826400338561868
1109	10	0.6324924955349687
1109	11	0.6214669184927502
1109	12	0.44824760667158614
1109	13	0.59257462049559
1109	14	0.7262906738072559
1109	15	0.5184662201982562
1109	16	0.10700074245631441
1109	17	0.7055698279454887
1109	18	0.6879825494511209
1109	19	0.6267474576576504
1109	20	0.590555357046064
1109	21	0.5730737542980293
1109	22	0.6073736905851277
1109	23	0.8230214120150379
1109	24	0.5880772683774254
1109	25	0.2517756740743238
1110	1	0.6665423421999996
1110	2	0.7288694825339803
1110	3	0.6865242280076806
1110	4	0.6863246167651695
1110	5	0.566498201334177
1110	6	0.4851729440252157
1110	7	0.4046856668777252
1110	8	0.5167081489575267
1110	9	0.6358675459755148
1110	10	0.6688744738527674
1110	11	0.6131553960559444
1110	12	0.5489951773730724
1110	13	0.64726056136886
1110	14	0.6334791013796064
1110	15	0.5496167448255633
1110	16	0.734800279537076
1110	17	0.7498881997217168
1110	18	0.2269087262021685
1110	19	0.6173105482857295
1110	20	0.5714187405454467
1110	21	0.7301834413394914
1110	22	0.860122792231742
1110	23	0.925135629847901
1110	24	0.7892859997439743
1110	25	1.0035948075688665
1111	1	0.38448509300223677
1111	2	0.3569942489524358
1111	3	0.4217608388422356
1111	4	0.3904694159259634
1111	5	0.3634796226893542
1111	6	0.26276623415306943
1111	7	0.19808124786491987
1111	8	0.43186689368856435
1111	9	0.49632447536254176
1111	10	0.3326443513342596
1111	11	0.23471039488347856
1111	12	-0.20893643843042042
1111	13	0.2649607127740765
1111	14	0.2769353963123109
1111	15	0.299500434774032
1111	16	0.5185613058187073
1111	17	-0.07707202648087019
1111	18	0.2414302752646975
1111	19	0.35695382599863734
1111	20	0.5162718013585916
1111	21	0.39928060514920105
1111	22	0.12922017298262745
1111	23	0.4265966300374231
1111	24	0.46572859444717807
1111	25	0.5654838839694349
1112	1	0.2934324818079297
1112	2	0.22081178237349164
1112	3	0.2165552401355697
1112	4	0.37474598226801703
1112	5	0.24529882684381477
1112	6	0.23676704297835016
1112	7	0.17139050517890553
1112	8	0.2868582244735265
1112	9	0.22827085225192434
1112	10	0.2152997086024513
1112	11	0.22616051794720704
1112	12	0.3839762349809135
1112	13	0.3502257210147022
1112	14	0.216685285673897
1112	15	0.08379347645841762
1112	16	0.2260908518286584
1112	17	0.5836371721274176
1112	18	0.3650027153592319
1112	19	0.545797718984403
1112	20	0.2636199691845249
1112	21	0.3003920812867946
1112	22	0.10687465438387278
1112	23	0.3201941311329932
1112	24	0.3398268004632783
1112	25	0.3088399269303608
1113	1	-0.08176306420996227
1113	2	-0.04119229358407139
1113	3	-0.009693981558388742
1113	4	0.10248642950019615
1113	5	-0.10008153531909869
1113	6	-0.3655082123777049
1113	7	-0.35367384558671944
1113	8	-0.16089252752080796
1113	9	-0.16537528402188428
1113	10	-0.04554930326550277
1113	11	-0.08370395518746676
1113	12	-0.29537846463271933
1113	13	-0.06401295479194147
1113	14	0.16655304973490104
1113	15	-0.12854470902770032
1113	16	-0.2594671653764422
1113	17	-0.1007552366716098
1113	18	-0.2971123213662068
1113	19	-0.6658380029605979
1113	20	-0.5437800399656609
1113	21	-0.29216541812757413
1113	22	-0.7099172579486748
1113	23	-4.392062370262799
1113	24	-1.821950013075707
1113	25	-0.9661351309504695
1114	1	-0.7971431608883499
1114	2	-1.04860934311073
1114	3	-0.8494314714193045
1114	4	-0.6481559965849151
1114	5	-0.7657541395773961
1114	6	-1.484205997507786
1114	7	-1.409671340532924
1114	8	-1.16173608290433
1114	9	-1.4810526492869371
1114	10	-1.1332899070933493
1114	11	-1.338469660984515
1114	12	-2.1620336126236026
1114	13	-1.4878146424397443
1114	14	-3.3091683977020345
1114	15	-2.039116684605901
1114	16	-1.9965807931541235
1114	17	-1.8736053322945168
1114	18	-1.8760735667712183
1114	19	-1.8841991508320959
1114	20	-1.5158946891393654
1114	21	-1.406629005514148
1114	22	-1.6639718903462821
1114	23	-5.7223232878916015
1114	24	-2.9407699732815367
1114	25	-2.720206204829111
1115	1	-1.759892223269354
1115	2	-1.6362733547116441
1115	3	-1.3921360914423602
1115	4	-1.414758190535121
1115	5	-1.389739033348686
1115	6	-1.6714607429813952
1115	7	-2.0572225770424275
1115	8	-2.3817447418613966
1115	9	-3.7745642736759883
1115	10	-4.459455501822866
1115	11	-3.1661273087736106
1115	12	-3.2621397109288495
1115	13	-2.5602585026653983
1115	14	-3.3431736869685764
1115	15	-2.9394590785467187
1115	16	-3.10277006960958
1115	17	-2.9019863681352973
1115	18	-5.393967249383927
1115	19	-3.442200079322671
1115	20	-3.9327457498616027
1115	21	-3.425713451210491
1115	22	-2.93905057731455
1115	23	-3.5277492199031393
1115	24	-3.34196876937353
1115	25	-3.5728393102127924
1116	1	-1.8058434594050894
1116	2	-1.605532844709142
1116	3	-2.041826884454185
1116	4	-1.825373675910754
1116	5	-1.9090659876571898
1116	6	-4.216238302445301
1116	7	-2.766285797544761
1116	8	-3.0731134633408175
1116	9	-3.99343223638159
1116	10	-3.286929184351898
1116	11	-2.9815501720931508
1116	12	-2.795943324289656
1116	13	-4.16656734757203
1116	14	-7.3654148652245155
1116	15	-5.145416491367598
1116	16	-4.132037546699937
1116	17	-5.020806088800036
1116	18	-3.827038286587804
1116	19	-3.422889418683596
1116	20	-4.357618089494089
1116	21	-5.050760867918424
1116	22	-4.617202577016368
1116	23	-3.6740685012777696
1116	24	-4.714539282091646
1116	25	-4.642122122521435
1201	1	0.6014433980662695
1201	2	0.5827494776445192
1201	3	0.9361045301315976
1201	4	0.7678882654446681
1201	5	0.45801920107621186
1201	6	0.7670739822199977
1201	7	0.8595704064172267
1201	8	0.8442831908332025
1201	9	1.2056740598956779
1201	10	1.0478270187929866
1201	11	1.236895714249414
1201	12	1.0182568761344086
1201	13	1.1396480705745091
1201	14	1.2943552705603807
1201	15	1.3040196382653306
1201	16	1.326975866803025
1201	17	1.4882562554406604
1201	18	1.320951478868139
1201	19	1.3753698715806095
1201	20	1.410030359581171
1201	21	1.4695847040876657
1201	22	1.1653805204498795
1201	23	1.5417202495663318
1201	24	1.357970217232122
1201	25	1.4706155732420925
1202	1	0.985577031366013
1202	2	0.9915276131293496
1202	3	0.9694645523730969
1202	4	1.1544066495169787
1202	5	1.009684497682585
1202	6	1.179249889503751
1202	7	1.2307069421079078
1202	8	1.1941155671276675
1202	9	1.437881695594534
1202	10	1.4502392152901322
1202	11	1.3176826385987113
1202	12	1.4990801499307693
1202	13	1.4143067630673087
1202	14	1.8942296612953413
1202	15	1.635652583196034
1202	16	1.429801062467888
1202	17	1.68983886985405
1202	18	1.7153843818623002
1202	19	1.6456669643148842
1202	20	1.5328906002977618
1202	21	1.7529017102198807
1202	22	1.78335250622262
1202	23	1.8821610906742805
1202	24	1.8742342019194993
1202	25	2.210089202975894
1203	1	1.2094211983517624
1203	2	1.3211644211876674
1203	3	1.208162584240931
1203	4	1.4306743256666223
1203	5	1.2085665926492482
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2211	18	-0.13255273352893227
2211	19	0.07992998364424153
2211	20	-0.02078049235628754
2211	21	0.14264894575391907
2211	22	0.17007761672444133
2211	23	0.293644083877453
2211	24	-0.003914101944879178
2211	25	0.5312730519988236
2212	1	0.26470143366472954
2212	2	0.4003733555520874
2212	3	-0.06634505033216698
2212	4	0.42814684768906375
2212	5	0.007573913751492967
2212	6	0.011062974435937178
2212	7	0.203886998960769
2212	8	0.2823018026106823
2212	9	0.3867319714500468
2212	10	0.0714683261222433
2212	11	-0.03650341021354109
2212	12	0.4787205635484497
2212	13	0.38201100088070206
2212	14	0.5266117049444716
2212	15	0.16692574230619073
2212	16	0.21457849199430257
2212	17	0.4083694698801139
2212	18	0.4984284400330381
2212	19	0.3221209424451601
2212	20	0.40654073197806895
2212	21	0.5051618673244036
2212	22	0.6058709786708363
2212	23	0.7552509950119699
2212	24	0.6074027574448024
2212	25	0.8493724732755865
2213	1	-0.41146464228378576
2213	2	-0.2643336939513361
2213	3	-0.3625383357550902
2213	4	-0.2771769456882751
2213	5	-0.3308982146516115
2213	6	-0.17313651031544453
2213	7	-0.326411502748833
2213	8	-0.17530592894104302
2213	9	-0.6067586494116881
2213	10	-0.2783301304067365
2213	11	-0.4629576837418483
2213	12	-0.48663590731658246
2213	13	-0.49141607644303065
2213	14	-0.6487681861207406
2213	15	-0.5620122435565946
2213	16	-0.5435644982380481
2213	17	-0.5221784414799373
2213	18	-0.4720475530506363
2213	19	-0.5921427788243392
2213	20	-0.351015015533676
2213	21	-0.46357419803563005
2213	22	-0.5904114252582175
2213	23	-0.39814741947481025
2213	24	-0.12500813300093938
2213	25	-0.03337518654742411
2214	1	-2.848787741259997
2214	2	-1.4738176407942127
2214	3	-1.6206515409322861
2214	4	-3.396632016631685
2214	5	-1.8496204460839287
2214	6	-1.5819258849546571
2214	7	-1.5055781915233304
2214	8	-1.2979278523832534
2214	9	-1.2815086260495718
2214	10	-1.2051940942435948
2214	11	-1.135333657979956
2214	12	-1.2097300324402838
2214	13	-1.6245099665920364
2214	14	-1.5026389394268733
2214	15	-1.71264878134199
2214	16	-1.5542310499800749
2214	17	-3.7374999393797275
2214	18	-3.647163581280953
2214	19	-2.428072726912255
2214	20	-2.3209275345631113
2214	21	-2.3170617368511195
2214	22	-3.1874201865658307
2214	23	-2.1686434120734326
2214	24	-3.1998062090671198
2214	25	-2.9666271052998923
2215	1	-2.0058328234677667
2215	2	-3.6331477346719616
2215	3	-2.179780560688389
2215	4	-3.875452014939885
2215	5	-2.3431064688645584
2215	6	-2.2486315019099656
2215	7	-2.328889863748774
2215	8	-1.7016771575360004
2215	9	-4.08602942744583
2215	10	-2.4380511119419133
2215	11	-2.1335665448847188
2215	12	-1.9716151573574194
2215	13	-2.061521523498733
2215	14	-1.8001249518495328
2215	15	-1.9190703136657632
2215	16	-2.013439092427042
2215	17	-2.3695396915479954
2215	18	-2.0510412821220214
2215	19	-2.091654444402039
2215	20	-1.9961789095838256
2215	21	-2.4650413281946193
2215	22	-2.3512207162034393
2215	23	-2.411364671292027
2215	24	-2.3080594191751196
2215	25	-3.2895909223159663
2216	1	-3.426679305918505
2216	2	-3.4212541976235222
2216	3	-4.703422353073449
2216	4	-5.192525021405402
2216	5	-4.952869848893297
2216	6	-3.9509024506989654
2216	7	-5.516773753834983
2216	8	-3.4853462744284442
2216	9	-4.285095198689671
2216	10	-3.159159935320403
2216	11	-3.4489625578157543
2216	12	-3.8364582659370576
2216	13	-3.327516302336992
2216	14	-4.429347742797805
2216	15	-3.897193068694502
2216	16	-4.182397874754628
2216	17	-3.822894961457685
2216	18	-3.475280878283762
2216	19	-4.1392991488355335
2216	20	-4.855296379308786
2216	21	-4.494142569984437
2216	22	-3.996970664889812
2216	23	-4.060316269711567
2216	24	-4.653375446987106
2216	25	-4.6363751613348505
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103061.0
3	1.0
4	922.0
5	5538.0
6	6902.0
7	6277.0
8	5695.0
9	5141.0
10	4682.0
11	4310.0
12	4025.0
13	4004.0
14	3989.0
15	3876.0
16	4129.0
17	4374.0
18	4984.0
19	6121.0
20	7715.0
21	10005.0
22	12370.0
23	14883.0
24	17296.0
25	19504.0
26	21737.0
27	24104.0
28	26912.0
29	31791.0
30	38433.0
31	47218.0
32	59205.0
33	75334.0
34	97146.0
35	127264.0
36	172782.0
37	262406.0
38	727961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19191925359025	18.096203335399004	12.543463063646104	24.16841434736464
2	15.54220924441938	19.69857711847474	41.06619254186541	23.693021095240464
3	19.708163934908406	25.117537193276227	28.40066756967416	26.773631302141204
4	12.636657122601177	15.827478591778716	36.97853751649419	34.557326769125915
5	13.6421864255156	38.42746704153811	33.976933640857744	13.953412892088549
6	32.62618583884873	35.222623577424734	18.05793927165364	14.093251312072896
7	29.39560021313846	31.34886199284464	20.85691811930679	18.39861967471011
8	26.50812480108666	35.39583945618425	20.32268756735136	17.77334817537774
9	28.186863601669454	14.379455803303182	19.63725295158907	37.7964276434383
10	17.21569483284526	27.8063573107043	32.24475087929433	22.733196977156112
11	35.12764322624585	22.224821788720543	23.75480482781791	18.89273015721569
12	24.76771464506757	24.21929483515937	30.20486182663133	20.808128693141732
13	29.887027354290357	21.477794462965058	25.37563239763773	23.259545785106855
14	22.083130328867234	22.155653674380837	26.640073081607795	29.121142915144134
15	24.308855329940222	30.160525724157218	22.844224935966825	22.686394009935736
16	23.02271348929033	27.388494301961767	26.851754579619012	22.73703762912889
17	22.202618754799705	28.128951112415002	26.905251467764508	22.763178665020785
18	22.254814527561873	26.340583443435996	29.280134163667288	22.124467865334843
19	24.194177910981065	26.135043433464194	27.097761457273293	22.57301719828144
20	25.09965538068	25.8534022910333	27.34409655431434	21.70284577397236
21	24.556716893577544	25.892402223976973	26.277426714924555	23.273454167520924
22	24.732225792206606	27.040905930065527	26.229639668448275	21.99722860927959
23	23.437710155740472	26.842429825429576	27.212024778392752	22.5078352404372
24	24.13902483463623	25.680677523266137	27.60403846417368	22.576259177923955
25	23.5601467145294	26.49667040033862	27.45345132887086	22.489731556261123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	277.0
1	277.0
2	634.5
3	992.0
4	992.0
5	992.0
6	1840.5
7	2689.0
8	2689.0
9	2689.0
10	2913.5
11	3138.0
12	3138.0
13	3138.0
14	4244.0
15	5350.0
16	5350.0
17	5350.0
18	11058.5
19	16767.0
20	16767.0
21	16767.0
22	27800.5
23	38834.0
24	38834.0
25	38834.0
26	59891.0
27	80948.0
28	80948.0
29	80948.0
30	105125.0
31	129302.0
32	129302.0
33	129302.0
34	147492.0
35	165682.0
36	165682.0
37	165682.0
38	178910.5
39	192139.0
40	192139.0
41	192139.0
42	208683.5
43	225228.0
44	225228.0
45	225228.0
46	246531.5
47	267835.0
48	267835.0
49	267835.0
50	262999.0
51	258163.0
52	258163.0
53	258163.0
54	232361.0
55	206559.0
56	206559.0
57	206559.0
58	181789.5
59	157020.0
60	157020.0
61	157020.0
62	133033.0
63	109046.0
64	109046.0
65	109046.0
66	84706.5
67	60367.0
68	60367.0
69	60367.0
70	45640.5
71	30914.0
72	30914.0
73	30914.0
74	22135.5
75	13357.0
76	13357.0
77	13357.0
78	9198.5
79	5040.0
80	5040.0
81	5040.0
82	3435.0
83	1830.0
84	1830.0
85	1830.0
86	1154.0
87	478.0
88	478.0
89	478.0
90	297.0
91	116.0
92	116.0
93	116.0
94	68.5
95	21.0
96	21.0
97	21.0
98	13.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3364438970294058
2	0.009279462419951959
3	0.008518850746185407
4	0.009685121979294123
5	0.01120634532682723
6	0.07864724706746169
7	0.07844441728779061
8	0.10232762384406041
9	0.08280525755071885
10	0.08960005516970007
11	0.08534062979660736
12	0.09588777833950358
13	0.08214606076678785
14	0.08605053402545615
15	0.08285596499563663
16	0.0852899223516896
17	0.09756112402179001
18	0.09969083670833637
19	0.09340311353853284
20	0.09162835296641088
21	0.07976281085565264
22	0.09974154415325412
23	0.09289603908935515
24	0.08843378393659136
25	0.08823095415692027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1972097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.32674877685922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88015487344346	34.73734186610635
2	14.366092942567022	13.885331290808933
3	5.528296465018669	8.014937832868682
4	2.733846669883092	5.2847168483957345
5	1.488032049628563	3.595587551715724
6	0.9491305798615352	2.7521037053641875
7	0.6178017350230735	2.089944446965747
8	0.4564140061819973	1.7645604011997804
9	0.32629292112287656	1.4191808424095513
>10	1.5022995759377762	12.542058334404446
>50	0.08384465658964456	2.794130003975793
>100	0.05739809235095466	5.226501709831599
>500	0.0063645504437187	2.1619659754794567
>1k	0.003924806106959865	3.3766941434256124
>5k	1.06075840728645E-4	0.3549450470484127
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6924	0.3510983486106413	No Hit
GTCCTACAGTGGACATTTCTAAATT	4124	0.2091175028408846	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4054	0.20556798169664067	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3962	0.2009028967642058	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3829	0.19415880659014237	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3701	0.18766825364066778	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3434	0.17412936584762312	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2754	0.13964830330353933	No Hit
GGATACCACGTGTCCCGCCCTACTC	2193	0.11120142670467022	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2068	0.10486299608994892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.070744491777027E-5
7	0.0	0.0	0.0	0.0	1.0141488983554054E-4
8	0.0	0.0	0.0	0.0	1.0141488983554054E-4
9	0.0	0.0	0.0	0.0	1.5212233475331082E-4
10	0.0	0.0	0.0	0.0	1.5212233475331082E-4
11	0.0	0.0	0.0	0.0	1.5212233475331082E-4
12	0.0	0.0	0.0	0.0	1.5212233475331082E-4
13	0.0	0.0	0.0	0.0	1.5212233475331082E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACCG	70	7.272023E-6	13.570142	5
TAGGACC	610	0.0	13.0810585	4
GGTATCA	2045	0.0	12.617832	1
GGCGTGT	85	3.9490114E-6	12.292952	8
TATGCGA	55	0.0030728802	12.088841	12
GTCTTAT	120	9.687938E-9	11.901967	1
CCGTATG	80	2.872294E-5	11.87297	9
AGACCCG	125	1.8349965E-8	11.398919	5
AGATCGT	60	0.0058807563	11.082282	6
ATAGGCG	60	0.0058807563	11.082282	5
CGAGACG	60	0.0058818674	11.082001	18
GTCCTAC	2145	0.0	10.919847	1
GTATGCG	70	0.0014938379	10.855287	11
AGGACCT	1230	0.0	10.811983	5
TGTAGGA	2035	0.0	10.689675	2
CTACACG	80	3.7740608E-4	10.686758	4
GTCCTAT	125	2.171746E-7	10.664164	1
CTTAGAC	125	2.2428321E-7	10.639261	3
GGACCTG	1250	0.0	10.562999	6
CTAATAC	135	5.69471E-8	10.554822	3
>>END_MODULE