FastQCFastQC Report
Thu 26 May 2016
SRR1512829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972097
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87440.44338589836098324No Hit
TATCAACGCAGAGTACTTTTTTTTT64930.32924343985108234No Hit
GGTATCAACGCAGAGTACTTTTTTT51800.26266456467405No Hit
GTCCTACAGTGGACATTTCTAAATT38490.19517295548849778No Hit
CTGTAGGACGTGGAATATGGCAAGA36920.18721188663640784No Hit
GTACTGGTTCACTATCGGTCAGTCA34360.17423078073745865No Hit
GTCCTAAAGTGTGTATTTCTCATTT32460.1645963662030823No Hit
CTTTAGGACGTGAAATATGGCGAGG30170.1529843613169129No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29680.15049969651594217No Hit
GTCCTACAGTGTGCATTTCTCATTT20200.10242903873389594No Hit
GGATACCACGTGTCCCGCCCTACTC20130.10207408661947155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.012.5718091
TGTAGGA19850.011.5002922
TAGGACC7200.011.0944474
GGACCGT1201.2586315E-711.0941656
CCCGTAC3300.010.93120416
GGCGAGG9750.010.806190519
GTAGGAC20250.010.7564423
CTGTAGG20350.010.6584681
GTACTAA1252.1983942E-710.6547291
TCTTACA2500.010.6531052
CCCCGTA3300.010.6438115
AATGTCC5200.010.5962828
GATATAC3250.010.5376431
CCGTACA3450.010.45540317
AAATGTC4650.010.4255657
TGGCGAG20150.010.41076218
TAGGACA6500.010.3873274
TAAAACG1303.8804865E-710.22336912
GTCTTAC1409.496216E-810.1926611
GTATCAA40650.010.1333181
Produced by FastQC (version 0.11.5)