##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512829_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1972097
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.075617477233624	31.0	30.0	34.0	26.0	34.0
2	30.095240244267902	31.0	30.0	34.0	26.0	34.0
3	30.293806541970298	31.0	30.0	34.0	26.0	34.0
4	33.88183137036363	37.0	35.0	37.0	30.0	37.0
5	33.43451361672372	37.0	35.0	37.0	28.0	37.0
6	33.286001652048554	37.0	35.0	37.0	27.0	37.0
7	33.12800536687597	37.0	35.0	37.0	26.0	37.0
8	33.07506223071177	37.0	35.0	37.0	26.0	37.0
9	34.374632687945876	39.0	35.0	39.0	26.0	39.0
10	34.307416927260675	39.0	35.0	39.0	26.0	39.0
11	34.28340086719872	39.0	35.0	39.0	25.0	39.0
12	34.144160251752325	39.0	35.0	39.0	25.0	39.0
13	34.12480369880386	39.0	35.0	39.0	25.0	39.0
14	35.19065492214632	39.0	35.0	41.0	25.0	41.0
15	35.18617897598343	40.0	35.0	41.0	25.0	41.0
16	35.17821892128024	40.0	35.0	41.0	25.0	41.0
17	35.17262335473357	40.0	35.0	41.0	25.0	41.0
18	35.104104919788426	40.0	35.0	41.0	25.0	41.0
19	35.09967815984711	40.0	35.0	41.0	24.0	41.0
20	35.077135658134466	40.0	35.0	41.0	24.0	41.0
21	35.041543595472234	40.0	35.0	41.0	24.0	41.0
22	34.997719686202046	40.0	35.0	41.0	24.0	41.0
23	34.839007411907225	40.0	35.0	41.0	22.0	41.0
24	34.78457702638359	40.0	35.0	41.0	21.0	41.0
25	34.72655706083423	39.0	35.0	41.0	20.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5809248100198481
1101	2	0.46593941191586197
1101	3	0.40473126745106214
1101	4	0.4247934628722092
1101	5	0.414719455838906
1101	6	0.3959556975348093
1101	7	0.47719165211864834
1101	8	0.36110731687319486
1101	9	0.6420408357736065
1101	10	0.2640939155928166
1101	11	0.6062143790319183
1101	12	0.6399616266506953
1101	13	0.6861611713149003
1101	14	0.7846083884993718
1101	15	0.4881511135189811
1101	16	0.8061597037518524
1101	17	0.8292429320545338
1101	18	0.6197186272240245
1101	19	0.5402244865488655
1101	20	0.5542935874278498
1101	21	0.7409064401689918
1101	22	0.6734711316089417
1101	23	0.9020278183882127
1101	24	0.8040317826740875
1101	25	0.816802747587893
1102	1	0.70314079396735
1102	2	0.8293414277612783
1102	3	0.5150457836088904
1102	4	0.6520919943684547
1102	5	0.8403606096479876
1102	6	0.8365755539334927
1102	7	0.8001500105935193
1102	8	0.8641394754273293
1102	9	0.9589670107015706
1102	10	0.8033111420380266
1102	11	0.9059990841707943
1102	12	0.9366070613642279
1102	13	0.9549231544554928
1102	14	0.8823626988595876
1102	15	1.0284693607403241
1102	16	1.0709099010162149
1102	17	1.1744569058692562
1102	18	0.500936025048226
1102	19	0.8721945237880888
1102	20	1.093439405958918
1102	21	1.002253157725697
1102	22	0.7394141088191049
1102	23	1.0528806074028196
1102	24	0.9784384721674826
1102	25	1.0334548053854604
1103	1	0.7373093792590311
1103	2	0.9310637510746602
1103	3	0.6456429464861451
1103	4	0.6490526509907468
1103	5	0.8037913008098414
1103	6	0.8068411042778934
1103	7	0.8560669036019064
1103	8	0.9153952086753847
1103	9	0.9728177449796931
1103	10	0.9073743889980364
1103	11	1.0261298638580456
1103	12	1.1367593733253258
1103	13	1.0640006499537975
1103	14	1.2358672746832724
1103	15	1.2223870752161616
1103	16	1.1666235734929202
1103	17	1.136066986386858
1103	18	1.0949906004840173
1103	19	1.099733681408992
1103	20	1.063034550939733
1103	21	1.0887216579398995
1103	22	1.1179191371825752
1103	23	1.289738636622232
1103	24	1.2374045782307377
1103	25	1.09030877898644
1104	1	0.5897665714496974
1104	2	0.7719968055312165
1104	3	0.6637873983303528
1104	4	0.5135263263610454
1104	5	0.6058169926339261
1104	6	0.4878988848476169
1104	7	0.44088364191935625
1104	8	0.5316279954807754
1104	9	0.6326524974716534
1104	10	0.7420799559238418
1104	11	0.7380798541581086
1104	12	0.7346668316974672
1104	13	0.6320339723974442
1104	14	0.8572009282685968
1104	15	0.9164177908533944
1104	16	0.7330671135427522
1104	17	0.8055631521810867
1104	18	0.4554511880736385
1104	19	0.8877382357618302
1104	20	0.761182282287507
1104	21	0.7693993142328068
1104	22	0.8149206169483278
1104	23	0.8055598268342834
1104	24	0.982445431355174
1104	25	0.7774757372111907
1105	1	0.546463615525024
1105	2	0.5588592264991838
1105	3	0.634162888169179
1105	4	0.4153142121415456
1105	5	0.5207630844235567
1105	6	0.5843947474163329
1105	7	0.6089472294528591
1105	8	0.6543379652417087
1105	9	0.8580552423122114
1105	10	0.5634788675499038
1105	11	0.8162884696770618
1105	12	0.8622670966524382
1105	13	0.9207811470826215
1105	14	1.0039936200932615
1105	15	0.9653809221032219
1105	16	0.9705755490218237
1105	17	0.7238400649908172
1105	18	0.6477084143636134
1105	19	0.9768489557304818
1105	20	0.9389795618506938
1105	21	0.9028154944154707
1105	22	0.9150819182491148
1105	23	1.118032175479165
1105	24	1.1427932013589839
1105	25	0.9474867479496751
1106	1	0.1234006658304132
1106	2	0.3551408931041635
1106	3	-1.4316623207222499
1106	4	-0.3021605807112451
1106	5	0.04915672730036391
1106	6	0.18520727669048398
1106	7	0.2939899819486911
1106	8	0.37838888706116336
1106	9	0.5494398241868907
1106	10	0.31303526174837515
1106	11	0.40345355314642717
1106	12	0.5316920746263634
1106	13	0.458779690447507
1106	14	0.5690342497476095
1106	15	0.5798905648660124
1106	16	0.5086673655317426
1106	17	0.49973692297272265
1106	18	0.6200310709529475
1106	19	0.5919140895269308
1106	20	0.7338546864453264
1106	21	0.7472524798444411
1106	22	0.6093247849274377
1106	23	0.6791707544856678
1106	24	0.6256464704710396
1106	25	0.7949731377280429
1107	1	0.6620601659010745
1107	2	0.7821642483658202
1107	3	0.6833964207469023
1107	4	0.5216822556560388
1107	5	0.7264125866308433
1107	6	0.6927055329099687
1107	7	0.7139458973536819
1107	8	0.6368980800896864
1107	9	0.7549164347334596
1107	10	0.775141657433835
1107	11	0.8095025340035917
1107	12	1.0414105167579208
1107	13	0.8766863788145542
1107	14	0.9481868408003038
1107	15	0.8219478155737789
1107	16	0.969121158767237
1107	17	0.8877998905452387
1107	18	1.0205307267557515
1107	19	0.9856016040584308
1107	20	1.03125132471002
1107	21	0.9249676486834275
1107	22	1.0528912605760539
1107	23	1.1696848164229294
1107	24	1.2049052839708665
1107	25	1.1484705004024747
1108	1	0.5166667238713814
1108	2	0.5508322096111797
1108	3	0.5299662776897271
1108	4	0.37407145325453683
1108	5	0.6636429849185959
1108	6	0.5577244286420395
1108	7	0.5504151971080304
1108	8	0.4594681899565458
1108	9	0.6667072880299614
1108	10	0.7019331728663758
1108	11	0.6711597471704351
1108	12	0.7670044408544641
1108	13	0.7382286626629337
1108	14	0.9624035776109778
1108	15	0.8956588673599768
1108	16	0.8225043557827334
1108	17	0.8824702919507956
1108	18	0.9550746025143724
1108	19	0.9433904315765673
1108	20	1.031469153548052
1108	21	0.8789889619079716
1108	22	0.9105932548184583
1108	23	1.135294405107203
1108	24	0.9823812452512186
1108	25	0.9673987626353906
1109	1	0.10724356325135531
1109	2	-0.08760334294014527
1109	3	0.13856307042259886
1109	4	0.0997973449458982
1109	5	-0.33817802158107213
1109	6	0.08140747706032414
1109	7	0.024751320871111204
1109	8	0.14444921067672567
1109	9	0.29113635526331905
1109	10	-0.11683861823241415
1109	11	0.1473332182662901
1109	12	0.4103565913878171
1109	13	0.31267709976188485
1109	14	0.4201051318234832
1109	15	0.36580137536698487
1109	16	0.3127472841875658
1109	17	0.3936418420023742
1109	18	0.5576950344544045
1109	19	0.45113558791450004
1109	20	0.523933638142239
1109	21	0.43687735959761653
1109	22	0.5407093305315129
1109	23	0.47141497973060353
1109	24	0.5655513031057424
1109	25	0.523977502334688
1110	1	0.36723718892381285
1110	2	0.1518125268038517
1110	3	0.27736770815095113
1110	4	0.27010985297308565
1110	5	0.3597821936048433
1110	6	0.36430195131266174
1110	7	0.3623097044554555
1110	8	0.270401852545028
1110	9	0.35957798037303235
1110	10	0.4238114525253849
1110	11	0.3002494039603718
1110	12	0.4810146093078558
1110	13	0.3160671908242705
1110	14	0.345997745151557
1110	15	0.4226443325649356
1110	16	0.3825703758510315
1110	17	0.42308358843375515
1110	18	0.5192717301025951
1110	19	0.5693825253326708
1110	20	0.5491606420541686
1110	21	0.5976035784753506
1110	22	0.4977000077316944
1110	23	0.738111331346893
1110	24	0.7279553769847098
1110	25	0.53346855199635
1111	1	0.15222117176258365
1111	2	0.10308024226933199
1111	3	-0.0022644345632087948
1111	4	0.15711249314716014
1111	5	0.38945846412575236
1111	6	0.32863840024993607
1111	7	0.26361212064762896
1111	8	0.3092415209879178
1111	9	0.2156235096197605
1111	10	0.20028049432975337
1111	11	0.20569056468242053
1111	12	0.5059118630101054
1111	13	0.4739156589927873
1111	14	0.34344553086258856
1111	15	0.3030726691053829
1111	16	0.21869330970822887
1111	17	0.433135261848939
1111	18	0.24649877422586286
1111	19	-0.03985583562548811
1111	20	0.27892678538641036
1111	21	0.3582490297415859
1111	22	0.13881047831851134
1111	23	0.18793924769200743
1111	24	0.2653192537944662
1111	25	0.13206792061865258
1112	1	0.058780913850732475
1112	2	-0.19976721984966872
1112	3	0.020686130169455197
1112	4	0.1618757937912889
1112	5	-0.008831265582294634
1112	6	-0.013911884441220934
1112	7	0.16272325823463518
1112	8	0.05502865995821793
1112	9	0.2067140455579306
1112	10	-0.2292341377599314
1112	11	0.05196352964833295
1112	12	0.23976224291616433
1112	13	0.16038448786136428
1112	14	-0.03303983036322222
1112	15	-0.2428210877425485
1112	16	-0.19897834301042394
1112	17	-0.25298980644607383
1112	18	0.04340420163222092
1112	19	0.12583351687979416
1112	20	0.0011115219450985592
1112	21	-0.05263342537539728
1112	22	0.020673819875753452
1112	23	0.22301914425516856
1112	24	0.21462002493092314
1112	25	0.08842762885173983
1113	1	-0.6934379578838445
1113	2	-0.600806551720936
1113	3	-0.49885040686887905
1113	4	-2.18925171173564
1113	5	-0.8255922518469987
1113	6	-0.5530468106535906
1113	7	-0.4986618251116113
1113	8	-0.39142447350042886
1113	9	-0.36713926984417355
1113	10	-0.45844529132974543
1113	11	-0.6462663800416593
1113	12	-0.22557748926605115
1113	13	-0.3794368007012352
1113	14	-0.5365385995057252
1113	15	-0.6453822766859005
1113	16	-0.8355365681565203
1113	17	-0.9834218033609332
1113	18	-0.6901478919054895
1113	19	-0.6251113972154414
1113	20	-0.34251921020157994
1113	21	-0.5407472120986299
1113	22	-0.3811608519181604
1113	23	-0.23227550655614237
1113	24	-0.22343751562002012
1113	25	-0.5013151074985274
1114	1	-1.1770762056433313
1114	2	-2.2393737427455385
1114	3	-1.648219403068957
1114	4	-1.1868185377841485
1114	5	-1.179689082668908
1114	6	-0.6943228182984669
1114	7	-0.6405927035555692
1114	8	-0.736347432975279
1114	9	-1.1825520429333523
1114	10	-1.2493715147277555
1114	11	-1.1439524894481465
1114	12	-1.1448537418807305
1114	13	-1.179418541569035
1114	14	-1.3084783204095203
1114	15	-1.3098265205461104
1114	16	-1.7058442655167738
1114	17	-1.786761344936913
1114	18	-1.5075015239959555
1114	19	-1.9055942851657193
1114	20	-1.2187271478429196
1114	21	-1.4215065069573782
1114	22	-1.16269425962372
1114	23	-1.0972520123820289
1114	24	-1.1502044532888362
1114	25	-1.737604492436354
1115	1	-0.9683466206994851
1115	2	-1.1393405674642523
1115	3	-0.6873577846785857
1115	4	-0.500374395881579
1115	5	-0.7330686318815225
1115	6	-0.6136929052531741
1115	7	-0.5529307784503317
1115	8	-0.7911920151284377
1115	9	-1.8470881888628625
1115	10	-1.48147882320049
1115	11	-1.7843129315765793
1115	12	-2.4556139044187475
1115	13	-2.333839043475571
1115	14	-1.9693270025266827
1115	15	-2.1680748030993584
1115	16	-1.9546665951935225
1115	17	-1.8622958268861183
1115	18	-2.221797149136137
1115	19	-2.7109725407510084
1115	20	-2.858823022319342
1115	21	-2.0740550049413002
1115	22	-2.05362082019208
1115	23	-1.9136914075069313
1115	24	-2.5481511328917748
1115	25	-2.574607137069627
1116	1	-1.560726827618435
1116	2	-1.72153797645133
1116	3	-1.768793863974441
1116	4	-1.367729230468889
1116	5	-1.865120409542154
1116	6	-2.514627182128411
1116	7	-2.044130919023168
1116	8	-2.274850955613946
1116	9	-3.4328854338482984
1116	10	-2.2865617026007143
1116	11	-3.316984827126703
1116	12	-5.332059754591533
1116	13	-4.27462486691822
1116	14	-3.643842958075851
1116	15	-4.623900109404367
1116	16	-4.140983320698442
1116	17	-3.9692915617785296
1116	18	-3.759731580428504
1116	19	-4.128769807035358
1116	20	-5.962275150347118
1116	21	-4.791667929122493
1116	22	-3.998047267053952
1116	23	-4.916027578920591
1116	24	-4.998339255552224
1116	25	-4.137750537334796
1201	1	0.49227876659256964
1201	2	0.6939184057947188
1201	3	0.7019162512621193
1201	4	0.6008639853568027
1201	5	0.11950012735390914
1201	6	0.6383267334331322
1201	7	0.7625761570565999
1201	8	0.7082216842852631
1201	9	0.7442822291742246
1201	10	0.8640981823765159
1201	11	0.5787958915322449
1201	12	0.964573902435724
1201	13	1.063215153578625
1201	14	0.9662199730202943
1201	15	0.7661222510976984
1201	16	0.7964597692661712
1201	17	0.7602715023478765
1201	18	0.9090908817518937
1201	19	0.9600823236371951
1201	20	1.0651171082874527
1201	21	0.6107259665063367
1201	22	0.6279102346723064
1201	23	0.7914032109210254
1201	24	0.4258590677850904
1201	25	0.6035300477342247
1202	1	0.921043978324203
1202	2	1.018540559636353
1202	3	0.9277373581150243
1202	4	0.7898311379663809
1202	5	0.8861602720438455
1202	6	0.9598943539778517
1202	7	1.0488043465536379
1202	8	0.9157671216505818
1202	9	1.1755309117015358
1202	10	1.2369533591009372
1202	11	1.198257247484591
1202	12	1.23071518269753
1202	13	1.2542821378410736
1202	14	1.3584313972530637
1202	15	1.2894848398372076
1202	16	1.2691189973178894
1202	17	1.2232640701881223
1202	18	1.2002800028276042
1202	19	1.317630443248909
1202	20	1.2708721183564506
1202	21	1.3963650634640885
1202	22	1.1877413577678269
1202	23	1.3779161192040021
1202	24	1.4564739610862176
1202	25	1.548717619117042
1203	1	0.9856103624882699
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2211	10	-0.04329575064181057
2211	11	0.17285443272020018
2211	12	-0.028071570369760934
2211	13	0.048754698962476084
2211	14	0.020740598089084017
2211	15	0.0021032856515503795
2211	16	-0.06316285476341221
2211	17	-0.23342042722901368
2211	18	-0.15814599508602356
2211	19	0.049254098937197455
2211	20	-0.0018946542589617366
2211	21	-0.04553186987908475
2211	22	0.0243224323603215
2211	23	-0.13774044240902583
2211	24	0.09318852287537993
2211	25	-0.11271349605824099
2212	1	-0.00772091071207015
2212	2	-0.03406102431236846
2212	3	0.04853979292112243
2212	4	0.12245327290353458
2212	5	-0.044927033527152105
2212	6	0.18322398400414386
2212	7	-0.04923178241091364
2212	8	0.24569048771151358
2212	9	0.1800568174310797
2212	10	0.002744565703750368
2212	11	0.15398616885018157
2212	12	0.3487175560829243
2212	13	0.3304543463497325
2212	14	0.3279976113936769
2212	15	0.28749855970804816
2212	16	0.15990219299228414
2212	17	0.13049407824421166
2212	18	-0.04660756330825677
2212	19	0.3048832819133267
2212	20	0.08336972583085611
2212	21	0.25428861361508126
2212	22	0.34172043429811083
2212	23	-0.10144063227743061
2212	24	-0.1343400739743359
2212	25	0.09501798957678176
2213	1	-0.536130673011467
2213	2	-0.1427683299446123
2213	3	-0.1632430384860868
2213	4	-0.31566476161950874
2213	5	-0.1818343117350807
2213	6	-0.12716738967832697
2213	7	-0.6091405449443172
2213	8	-0.7863362516317096
2213	9	-0.6201257375423594
2213	10	-0.5552195345563575
2213	11	-0.31460845695214346
2213	12	-0.5287094277200879
2213	13	-0.3515498237476038
2213	14	-0.3979125358361628
2213	15	-0.7831666653914766
2213	16	-1.2417397064809421
2213	17	-0.9929758100848858
2213	18	-0.5910597098720771
2213	19	-0.5226119572711809
2213	20	-0.5308890034542557
2213	21	-0.2605106190937505
2213	22	-0.4760044172114135
2213	23	-0.6165011542957686
2213	24	-0.318296173157691
2213	25	-0.4477506787071235
2214	1	-1.3245054245149
2214	2	-0.6420629185856761
2214	3	-0.8048011611010786
2214	4	-0.8464444433901406
2214	5	-1.075083142248701
2214	6	-1.560150386931074
2214	7	-1.104430060297645
2214	8	-0.9426154305889725
2214	9	-0.7506892642504823
2214	10	-1.0416371850757855
2214	11	-1.1683360597760455
2214	12	-0.7786043658778823
2214	13	-1.2286720269396483
2214	14	-0.9442881486247217
2214	15	-1.090748540807894
2214	16	-1.0306375744591634
2214	17	-1.17375754382347
2214	18	-1.181495893289636
2214	19	-1.161699163960229
2214	20	-1.3445380446004904
2214	21	-1.5228768662457668
2214	22	-1.999377090217493
2214	23	-1.9486319297328336
2214	24	-1.7065012231212222
2214	25	-2.0814395312802105
2215	1	-1.331660732802657
2215	2	-0.8219372936547309
2215	3	-0.8596475912262029
2215	4	-0.9286723869530107
2215	5	-1.3454954671989796
2215	6	-1.2147072901738056
2215	7	-1.7717961058312852
2215	8	-1.434489386160184
2215	9	-1.4375972414422264
2215	10	-1.3922538728036642
2215	11	-1.1123219841559475
2215	12	-1.4837116226727147
2215	13	-1.58541394426922
2215	14	-1.7132270521298594
2215	15	-1.518032020361261
2215	16	-1.6649896358681247
2215	17	-1.6779189667670664
2215	18	-1.2403642374297874
2215	19	-1.5393660179335455
2215	20	-1.5475085381923606
2215	21	-1.2486649999809885
2215	22	-1.57780980828732
2215	23	-2.0089133817132776
2215	24	-1.941732482098125
2215	25	-2.076344488260098
2216	1	-1.652539286271459
2216	2	-1.0665326856908557
2216	3	-1.1537288848427885
2216	4	-1.395809535454653
2216	5	-2.2149952876012797
2216	6	-2.3022752807321005
2216	7	-2.3092197768082983
2216	8	-2.421952825199593
2216	9	-4.207307127012832
2216	10	-2.260252769560644
2216	11	-2.490508881220805
2216	12	-2.9691552289945555
2216	13	-2.88184123285707
2216	14	-2.9806461048802717
2216	15	-2.950800583066517
2216	16	-2.3550825776784734
2216	17	-2.510506007510088
2216	18	-2.99393726955212
2216	19	-3.0745302856880627
2216	20	-2.447865167913214
2216	21	-3.1017178401903465
2216	22	-2.4735879794695848
2216	23	-3.652749973462047
2216	24	-2.8800175806202937
2216	25	-3.083080043473565
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61223.0
3	2.0
4	1070.0
5	8548.0
6	10599.0
7	8121.0
8	6561.0
9	5553.0
10	4723.0
11	4195.0
12	3711.0
13	3595.0
14	3412.0
15	3353.0
16	3460.0
17	3687.0
18	4067.0
19	4846.0
20	6558.0
21	8506.0
22	10634.0
23	13085.0
24	15050.0
25	17053.0
26	19000.0
27	20557.0
28	23333.0
29	28130.0
30	34909.0
31	44068.0
32	56004.0
33	72370.0
34	94698.0
35	123115.0
36	169278.0
37	261711.0
38	813312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.8787211183059	19.180637116456534	13.489179150115534	26.45146261512204
2	13.82933336309489	20.12730168022593	39.82689934117043	26.21646561550875
3	17.529106389712194	25.008317560398673	29.35611939147917	28.106456658409968
4	11.949784404179157	16.442433152110798	36.17968160886258	35.42810083484746
5	13.243935467183308	38.53842603628323	34.081837592749444	14.135800903784023
6	32.70372982489966	35.83006489519857	17.585025902266512	13.881179377635258
7	28.876642782869233	31.49799062477639	20.991437255171622	18.633929337182753
8	26.410831434906363	35.929738752771534	19.844083757414012	17.815346054908087
9	27.452242448035147	14.32510995027828	19.4859637456995	38.73668385598707
10	16.590582085099733	28.448970397533905	32.206050373100254	22.754397144266107
11	34.8318600944543	22.23494504703191	23.789946951229716	19.143247907284078
12	24.214779353264483	24.54402412872847	30.291127834494286	20.95006868351276
13	29.624532256371428	21.36759895069238	25.520457492442826	23.487411300493367
14	21.727035459640692	22.01074706666538	26.815944440190602	29.446273033503324
15	24.120507700866877	30.224298619884156	22.817276273195283	22.837917406053684
16	22.88902085457984	27.300213551140963	26.82148574998745	22.989279844291755
17	21.97554441392243	28.163427372345502	27.0897365748082	22.771291638923866
18	22.086413166947942	26.207981062573023	29.525428404517722	22.180177365961313
19	23.918312747393838	26.07808695281782	27.445744496358625	22.557855803429717
20	24.817246198376107	25.739635668836318	27.598917269930002	21.844200862857573
21	24.12034769274768	25.630322409874616	26.73280982675198	23.516520070625724
22	24.315597104382977	26.90615612722379	26.69343699355606	22.084809774837176
23	23.112504100964607	26.64934838397908	27.59636062526336	22.641786889792947
24	23.947402181535697	25.631903501704024	27.80968684603242	22.61100747072786
25	23.2400333249328	26.414065839560628	27.78950528295515	22.556395552551418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	113.0
1	113.0
2	381.5
3	650.0
4	650.0
5	650.0
6	1459.0
7	2268.0
8	2268.0
9	2268.0
10	2592.0
11	2916.0
12	2916.0
13	2916.0
14	4149.5
15	5383.0
16	5383.0
17	5383.0
18	11468.5
19	17554.0
20	17554.0
21	17554.0
22	29101.5
23	40649.0
24	40649.0
25	40649.0
26	63121.0
27	85593.0
28	85593.0
29	85593.0
30	108747.5
31	131902.0
32	131902.0
33	131902.0
34	149600.0
35	167298.0
36	167298.0
37	167298.0
38	180109.0
39	192920.0
40	192920.0
41	192920.0
42	209482.5
43	226045.0
44	226045.0
45	226045.0
46	246585.0
47	267125.0
48	267125.0
49	267125.0
50	261504.0
51	255883.0
52	255883.0
53	255883.0
54	230862.5
55	205842.0
56	205842.0
57	205842.0
58	180491.0
59	155140.0
60	155140.0
61	155140.0
62	130808.5
63	106477.0
64	106477.0
65	106477.0
66	82445.0
67	58413.0
68	58413.0
69	58413.0
70	44210.0
71	30007.0
72	30007.0
73	30007.0
74	21445.0
75	12883.0
76	12883.0
77	12883.0
78	8875.5
79	4868.0
80	4868.0
81	4868.0
82	3244.5
83	1621.0
84	1621.0
85	1621.0
86	1030.0
87	439.0
88	439.0
89	439.0
90	270.5
91	102.0
92	102.0
93	102.0
94	53.0
95	4.0
96	4.0
97	4.0
98	3.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08559416702119621
2	0.044318306858131214
3	0.01850821739498615
4	0.0060848933901324325
5	0.019623781183177095
6	0.062218034914104124
7	0.08032059274974812
8	0.0591755882190379
9	0.026570701136911623
10	0.05298927993906994
11	0.006997627398652297
12	4.0565955934216216E-4
13	0.006591967839310136
14	0.0012169786780264866
15	0.015516478144837705
16	0.010344318763225134
17	0.005780648720625811
18	0.006541260394392365
19	0.0019775903517930404
20	0.002281835021299662
21	5.577818940954729E-4
22	6.591967839310136E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1972097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.089088123503075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.74533691463284	33.313334043000616
2	14.867597413696675	14.002032095966586
3	5.7589777930456485	8.135550383940714
4	2.81387513236238	5.30011256305384
5	1.6408185931799708	3.8632325664466998
6	0.976436714515393	2.7587708698103492
7	0.647599672929616	2.134641464713412
8	0.4601101549659604	1.7332934106968612
9	0.34301853983105113	1.4537187225089745
>10	1.5890825601642924	12.840956959016061
>50	0.0876247369153838	2.8737472707143494
>100	0.05923578167011448	5.40336972642975
>500	0.006279868855355929	2.162276028513207
>1k	0.003681302432450027	2.984002101409868
>5k	3.248208028632377E-4	1.040961793778688
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8744	0.44338589836098324	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6493	0.32924343985108234	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5180	0.26266456467405	No Hit
GTCCTACAGTGGACATTTCTAAATT	3849	0.19517295548849778	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3692	0.18721188663640784	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3436	0.17423078073745865	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3246	0.1645963662030823	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3017	0.1529843613169129	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2968	0.15049969651594217	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2020	0.10242903873389594	No Hit
GGATACCACGTGTCCCGCCCTACTC	2013	0.10207408661947155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	5.070744491777027E-5
10	0.0	0.0	0.0	0.0	5.070744491777027E-5
11	0.0	0.0	0.0	0.0	5.070744491777027E-5
12	0.0	0.0	0.0	0.0	5.070744491777027E-5
13	0.0	0.0	0.0	0.0	5.070744491777027E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1975	0.0	12.571809	1
TGTAGGA	1985	0.0	11.500292	2
TAGGACC	720	0.0	11.094447	4
GGACCGT	120	1.2586315E-7	11.094165	6
CCCGTAC	330	0.0	10.931204	16
GGCGAGG	975	0.0	10.8061905	19
GTAGGAC	2025	0.0	10.756442	3
CTGTAGG	2035	0.0	10.658468	1
GTACTAA	125	2.1983942E-7	10.654729	1
TCTTACA	250	0.0	10.653105	2
CCCCGTA	330	0.0	10.64381	15
AATGTCC	520	0.0	10.596282	8
GATATAC	325	0.0	10.537643	1
CCGTACA	345	0.0	10.455403	17
AAATGTC	465	0.0	10.425565	7
TGGCGAG	2015	0.0	10.410762	18
TAGGACA	650	0.0	10.387327	4
TAAAACG	130	3.8804865E-7	10.223369	12
GTCTTAC	140	9.496216E-8	10.192661	1
GTATCAA	4065	0.0	10.133318	1
>>END_MODULE