FastQCFastQC Report
Thu 26 May 2016
SRR1512828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421034
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69820.2883891758645273No Hit
GTCCTACAGTGGACATTTCTAAATT59010.2437388322510134No Hit
GTCCTAAAGTGTGTATTTCTCATTT53640.22155822677417994No Hit
CTGTAGGACGTGGAATATGGCAAGA50980.21057118569999428No Hit
CTTTAGGACGTGAAATATGGCGAGG40700.16810998936817906No Hit
TATCAACGCAGAGTACTTTTTTTTT39750.16418604612739846No Hit
GGTATCAACGCAGAGTACTTTTTTT38710.15989036089538602No Hit
GTACTGGTTCACTATCGGTCAGTCA32290.1333727655208477No Hit
GTCCTACAGTGTGCATTTCTCATTT27200.11234869068340221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20800.011.9588531
TAGGACC6950.011.8899784
GTTAGAC1001.9349754E-611.3979783
GTATCAA45600.011.2024311
AAATCGC600.005884119311.08159617
GGACGTC700.00149413110.8552176
ACACCGT1252.2470886E-710.6381136
TAGAAAT7700.010.6085084
GGCGAGG12000.010.52751719
GTCGAGG1002.4040413E-510.44836219
GTCCTAC28350.010.3876611
TAGACTC1752.3646862E-1010.3124575
GCGCCAC2500.010.25818113
GCGTGCG2600.010.2285329
CTTACAC2800.010.1767663
AGGACGT29900.010.1654215
TCCTACA29750.010.1528212
GTGTAAG3400.010.091041
CGTGCGC2450.010.07942910
TGTAGGA29800.010.0720392
Produced by FastQC (version 0.11.5)