##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512828_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2421034
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.088211896239375	31.0	30.0	34.0	23.0	34.0
2	29.216911864930438	31.0	30.0	34.0	23.0	34.0
3	29.38204585313548	31.0	30.0	34.0	23.0	34.0
4	32.80669251237281	35.0	33.0	37.0	26.0	37.0
5	33.01203741872274	37.0	35.0	37.0	26.0	37.0
6	32.90723178608809	37.0	35.0	37.0	26.0	37.0
7	32.87063130877138	37.0	35.0	37.0	26.0	37.0
8	32.765539847850135	37.0	35.0	37.0	25.0	37.0
9	34.10105723422306	39.0	35.0	39.0	25.0	39.0
10	33.97374881971918	39.0	34.0	39.0	24.0	39.0
11	34.02924865987012	39.0	35.0	39.0	25.0	39.0
12	33.931616408526274	39.0	34.0	39.0	23.0	39.0
13	33.90149498107007	39.0	34.0	39.0	23.0	39.0
14	34.77552607687459	39.0	34.0	41.0	21.0	41.0
15	34.9525764611319	39.0	35.0	41.0	24.0	41.0
16	34.89934342103415	39.0	35.0	41.0	23.0	41.0
17	34.66727150465462	39.0	34.0	41.0	19.0	41.0
18	34.67192158391828	39.0	34.0	41.0	19.0	41.0
19	34.78704181766964	39.0	34.0	41.0	21.0	41.0
20	34.756991847285086	39.0	34.0	41.0	19.0	41.0
21	34.657558299470395	39.0	34.0	41.0	18.0	41.0
22	34.669668414404754	39.0	34.0	41.0	19.0	41.0
23	34.33932567654977	39.0	34.0	41.0	17.0	41.0
24	34.409601847805526	39.0	34.0	41.0	17.0	41.0
25	34.12296440281301	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6132698715200817
1101	2	0.35065896339393277
1101	3	0.6775558906972314
1101	4	0.7821500219551112
1101	5	0.5579186066873021
1101	6	0.609775744518835
1101	7	0.3175404078974253
1101	8	0.6381393152875532
1101	9	0.6537922622264958
1101	10	0.785157749720554
1101	11	0.8104353667434054
1101	12	0.6258731092901542
1101	13	1.0262683144155673
1101	14	1.0375446127723151
1101	15	0.9616194233990569
1101	16	0.7671473997720781
1101	17	0.14644754134834415
1101	18	0.546387100885724
1101	19	1.7588348121932995E-5
1101	20	-0.6342646691445211
1101	21	0.5136927512966238
1101	22	0.6054604783581823
1101	23	0.2176553751709207
1101	24	0.6413435313340443
1101	25	0.29869142937808135
1102	1	0.8181620963947971
1102	2	0.8566813775980755
1102	3	0.8243458002072508
1102	4	0.9434040741907808
1102	5	0.8400941151299577
1102	6	0.7127662650402442
1102	7	0.670075021838791
1102	8	0.7090636045404679
1102	9	0.979730312242765
1102	10	1.0520502048025193
1102	11	0.8273521788932143
1102	12	1.0664189499332792
1102	13	1.0440910598188182
1102	14	1.1374961837230089
1102	15	1.0462007758274723
1102	16	0.9520984736033995
1102	17	1.2854929367221786
1102	18	1.221606715399048
1102	19	1.004418823199785
1102	20	1.220636625040754
1102	21	1.2687119643370508
1102	22	1.0169094762260897
1102	23	0.9155605247931646
1102	24	0.35484768130088185
1102	25	0.7725009353697061
1103	1	0.6965328571346916
1103	2	0.7627666406005247
1103	3	0.8954764270201565
1103	4	0.9244816952311652
1103	5	0.794591303158434
1103	6	0.43523385545660886
1103	7	0.3375387276344597
1103	8	0.4761149599821195
1103	9	0.7559769231271005
1103	10	0.7971754584161275
1103	11	0.5882724854539845
1103	12	0.815310423251546
1103	13	0.9040751245851979
1103	14	0.9168513235086593
1103	15	0.8127011192735836
1103	16	0.8661299899080888
1103	17	0.6054275366478663
1103	18	0.8502105499126102
1103	19	0.880751033769883
1103	20	1.089447563488953
1103	21	0.8622941450641264
1103	22	0.8589538833260164
1103	23	0.22499940050673217
1103	24	0.44362720013506873
1103	25	0.6877958811178502
1104	1	0.8859537372561981
1104	2	0.8767691337983834
1104	3	0.9451249385080231
1104	4	1.044422548451493
1104	5	0.7752109682073183
1104	6	0.7588585642402776
1104	7	1.0570923758685424
1104	8	0.7501100893502652
1104	9	1.0826798240159334
1104	10	1.1202252836269508
1104	11	0.7728595800099143
1104	12	1.0063118260570363
1104	13	1.134049521502
1104	14	1.3448382328288488
1104	15	1.1411324708668005
1104	16	1.1219810690056775
1104	17	1.394602286257637
1104	18	1.1885398467788377
1104	19	0.7026431186504354
1104	20	0.9244768780047536
1104	21	0.9542255061912357
1104	22	0.7172930574411609
1104	23	1.0530810516168714
1104	24	0.8705106207086146
1104	25	0.45935627134110746
1105	1	0.5694097740492126
1105	2	0.6247977988546758
1105	3	0.7445615084984425
1105	4	0.8627916977008283
1105	5	0.6130959059282475
1105	6	0.7286453693860153
1105	7	0.6573523707204103
1105	8	0.7446721948926438
1105	9	0.4000667140089078
1105	10	0.617948133551323
1105	11	0.5416605674912489
1105	12	0.7263954347818853
1105	13	0.7507978852119663
1105	14	0.9118206427398974
1105	15	0.8260936680446562
1105	16	0.8538166668575045
1105	17	1.1655193711912872
1105	18	0.9372972922855141
1105	19	0.6340481029171912
1105	20	0.9658061698492091
1105	21	0.797317735044011
1105	22	0.8559785445433192
1105	23	0.9567087330737252
1105	24	1.0261221146438402
1105	25	1.3222646737591646
1106	1	0.6230808947594042
1106	2	0.657606529097631
1106	3	0.6436686648915177
1106	4	0.7235207646527968
1106	5	0.5892800427905982
1106	6	0.6887623550314288
1106	7	0.6182956573313163
1106	8	0.5689310620109396
1106	9	0.7685163381431508
1106	10	0.5567163826947734
1106	11	0.48092660053191594
1106	12	0.7161630147088545
1106	13	0.724155245726898
1106	14	1.0433084826000751
1106	15	0.7515538626588736
1106	16	0.885349297116484
1106	17	0.9655525071526867
1106	18	0.8999165303100014
1106	19	0.9779733255561993
1106	20	1.0662775722150712
1106	21	1.0040080318382678
1106	22	0.8839077066799632
1106	23	1.201890347379802
1106	24	0.8688066337609044
1106	25	1.078395977171951
1107	1	0.7215581092507364
1107	2	0.7584194155525061
1107	3	0.6990948867020954
1107	4	0.8079166472006349
1107	5	0.7346506118716292
1107	6	0.776259356442786
1107	7	0.5005550656385083
1107	8	0.7184188412158505
1107	9	0.6620604574670779
1107	10	0.8903995289592004
1107	11	0.8748412405291646
1107	12	0.8655625843105312
1107	13	0.6579247594122961
1107	14	0.9838628073448064
1107	15	0.8372918489412058
1107	16	0.9105287014650045
1107	17	1.1796619559072141
1107	18	1.0823633705774753
1107	19	0.8258189539097813
1107	20	1.001515184144452
1107	21	0.6952242647718592
1107	22	0.7432966911292525
1107	23	1.067339371910677
1107	24	0.9896472685903603
1107	25	1.2258677097591573
1108	1	0.6760517328200848
1108	2	0.6337924880195374
1108	3	0.6234372108759452
1108	4	0.676633991400756
1108	5	0.553672180315175
1108	6	0.6559335213357897
1108	7	0.5910555999103195
1108	8	0.5156284076974416
1108	9	0.701686081913877
1108	10	0.7263969567747779
1108	11	0.6079551183254992
1108	12	0.7096329195701472
1108	13	0.5669428162040191
1108	14	0.6622999289941589
1108	15	0.6031180542180721
1108	16	0.25577992207859523
1108	17	0.760874385156896
1108	18	0.8206491188881628
1108	19	0.7249091675575343
1108	20	0.8920537407712317
1108	21	0.792363663079442
1108	22	0.8410434612073843
1108	23	1.0141335010307486
1108	24	0.9792718098346285
1108	25	1.130293960725119
1109	1	0.6956359977600854
1109	2	0.6756776878337867
1109	3	0.7347651249411733
1109	4	0.7873984738970847
1109	5	0.6365912815906682
1109	6	0.6798344930901408
1109	7	0.5800529507071417
1109	8	0.6101679775314892
1109	9	0.8052265714194959
1109	10	0.8631247440905625
1109	11	0.7296323806825953
1109	12	0.763628078021199
1109	13	0.8080486649370826
1109	14	0.9980866263113626
1109	15	0.802024678417375
1109	16	0.4479317126928706
1109	17	0.8551403326271014
1109	18	0.889271095542334
1109	19	0.8093640763271992
1109	20	0.7316421443627945
1109	21	0.7185347033442753
1109	22	0.8100978466629556
1109	23	1.1115738823046755
1109	24	0.7830204750677012
1109	25	0.41569568316042194
1110	1	0.6258433138030881
1110	2	0.5718811088137663
1110	3	0.5456663051009727
1110	4	0.49991338557052956
1110	5	0.3949956449678069
1110	6	0.5546956780299155
1110	7	0.390795485083828
1110	8	0.43268545432181327
1110	9	0.6115450517989274
1110	10	0.5411645878759757
1110	11	0.6211748091580702
1110	12	0.6029210586664107
1110	13	0.5881959994818899
1110	14	0.6043120825725197
1110	15	0.5718480613635677
1110	16	0.7508024802500088
1110	17	0.7625846829829186
1110	18	0.2373730378199923
1110	19	0.5589705523716901
1110	20	0.6516951001191629
1110	21	0.7937670576064875
1110	22	0.8116477335505721
1110	23	0.9103399477656353
1110	24	0.7348217743148524
1110	25	1.020540199991295
1111	1	0.40358410102080455
1111	2	0.4387621440703171
1111	3	0.5666299348275778
1111	4	0.4801453674407199
1111	5	0.4972169140960432
1111	6	0.380719874897423
1111	7	0.32111729132275
1111	8	0.5611074796861786
1111	9	0.6739633580285442
1111	10	0.5493743593816021
1111	11	0.34211431854044605
1111	12	0.07670000824997913
1111	13	0.5025258623811268
1111	14	0.38212506278194525
1111	15	0.30381487249378125
1111	16	0.466667151934125
1111	17	-0.0578458173345453
1111	18	0.30511792006721095
1111	19	0.4097651535509712
1111	20	0.47636171973435637
1111	21	0.5319846701629274
1111	22	0.14923560605865305
1111	23	0.49186257487183127
1111	24	0.5517650364401732
1111	25	0.7853222482506013
1112	1	0.2892303195837904
1112	2	0.22266011280152398
1112	3	0.19004287006433884
1112	4	0.30566105164984947
1112	5	0.2702054659823645
1112	6	0.09746322880427982
1112	7	-0.0421502783091654
1112	8	0.1042979848482588
1112	9	0.011783467205894738
1112	10	0.09804907425886711
1112	11	0.03146028938930101
1112	12	0.06290455892916924
1112	13	0.06373079190205999
1112	14	-0.024800486594998006
1112	15	-0.3017262763284734
1112	16	0.06828911043950825
1112	17	0.30494263840657965
1112	18	-0.07536441156020146
1112	19	0.10633368569883572
1112	20	-0.08599211586896871
1112	21	-0.20153843869942278
1112	22	-0.21362584523084394
1112	23	0.08556483879781496
1112	24	0.14553732966432165
1112	25	0.06676458096134041
1113	1	-0.08384607260827437
1113	2	0.04286049802787062
1113	3	-0.01209504017375096
1113	4	0.10781286419641845
1113	5	-0.049976746940757266
1113	6	-0.2948828221622435
1113	7	-0.29569356298110705
1113	8	-0.05420474976892109
1113	9	-0.15731362088938994
1113	10	-0.06979548204399322
1113	11	-0.10499841529289711
1113	12	-0.20593344971740635
1113	13	-0.12406913799058827
1113	14	0.23316359977010848
1113	15	-0.08301304090049655
1113	16	-0.18219043645115818
1113	17	-0.09027589429744154
1113	18	-0.24731491536993389
1113	19	-0.7078002617193206
1113	20	-0.4379471279987328
1113	21	-0.32313670104326775
1113	22	-0.7474957414699759
1113	23	-4.579432690232359
1113	24	-1.95317905695358
1113	25	-1.1002051229294167
1114	1	-0.7110010416999373
1114	2	-0.84840290680172
1114	3	-0.7387477559999915
1114	4	-0.6169509369823842
1114	5	-0.713106231755809
1114	6	-1.2983472824657696
1114	7	-1.2084398574898607
1114	8	-1.0085919444103233
1114	9	-1.2517052794929313
1114	10	-0.8759128359268971
1114	11	-0.9688022690268099
1114	12	-1.9352278704550372
1114	13	-1.1996272339266554
1114	14	-2.917098773055862
1114	15	-1.7168134747561936
1114	16	-1.8479605475785021
1114	17	-1.621839227335478
1114	18	-1.6974419311997408
1114	19	-1.6903153793642787
1114	20	-1.258745294207685
1114	21	-1.2137899783162922
1114	22	-1.3857305838449392
1114	23	-5.432529218811496
1114	24	-2.871576635484221
1114	25	-2.665092925613088
1115	1	-1.298787852199343
1115	2	-1.1866241734486103
1115	3	-0.9543992732647659
1115	4	-0.8896754830676876
1115	5	-0.9584871525043894
1115	6	-1.0750798176389154
1115	7	-1.4992576811105636
1115	8	-1.9084554538172114
1115	9	-3.145977373002726
1115	10	-3.8653298653667854
1115	11	-2.658687751675746
1115	12	-2.7731543038278907
1115	13	-2.034223403206905
1115	14	-2.685958351155435
1115	15	-2.384494266431183
1115	16	-2.452557804291928
1115	17	-2.279565573004483
1115	18	-4.933017484756416
1115	19	-2.7969201027248296
1115	20	-3.1865782201475774
1115	21	-2.890478292192057
1115	22	-2.3547001759038366
1115	23	-2.887525442345204
1115	24	-2.541570586637196
1115	25	-2.931170536427846
1116	1	-1.2454669443010147
1116	2	-1.0999948963126869
1116	3	-1.49144760263275
1116	4	-1.0576186609535263
1116	5	-1.071183550832064
1116	6	-3.474474947288634
1116	7	-2.0756218483682645
1116	8	-2.3687425365633885
1116	9	-3.354484457476687
1116	10	-2.6257740814726773
1116	11	-2.1141515816895264
1116	12	-1.9526916602346667
1116	13	-3.324466861930656
1116	14	-6.8406765734156885
1116	15	-4.382808210130673
1116	16	-3.192495398407413
1116	17	-4.133823891951884
1116	18	-2.8585894774579295
1116	19	-2.553760719493681
1116	20	-3.5192170660551447
1116	21	-4.137046531950585
1116	22	-3.660801904975031
1116	23	-2.6220678032747493
1116	24	-3.6889410851183655
1116	25	-3.532238216943817
1201	1	0.3354443860895948
1201	2	0.33818156246925213
1201	3	0.689241433760106
1201	4	0.5747732788754618
1201	5	0.09292531094408218
1201	6	0.5076304185010798
1201	7	0.6697823120871078
1201	8	0.5365095789714474
1201	9	1.0700701759933793
1201	10	0.8774271216605669
1201	11	0.9263814478170076
1201	12	0.8971769019581686
1201	13	1.0319586721072795
1201	14	1.0988908940517632
1201	15	0.9792625544912212
1201	16	0.9761974414725927
1201	17	1.3462012787591107
1201	18	1.0757840648280705
1201	19	1.145315914218017
1201	20	1.0957449145625233
1201	21	1.221170609535129
1201	22	1.0477987727330742
1201	23	1.3682014946371979
1201	24	1.185237234972277
1201	25	1.2033808662964347
1202	1	0.817730188243317
1202	2	0.7737295150012145
1202	3	0.8345060661646251
1202	4	0.9449168092477365
1202	5	0.7824348699535477
1202	6	1.0901936265343366
1202	7	1.0322875523451245
1202	8	1.1339202144854994
1202	9	1.2231486609998612
1202	10	1.112515796436739
1202	11	1.0540086597024967
1202	12	1.3224987861688149
1202	13	1.258622205277689
1202	14	1.6814520299189084
1202	15	1.3305360827763266
1202	16	1.2030816246072078
1202	17	1.4930728402584634
1202	18	1.5275869458106328
1202	19	1.5296592129792899
1202	20	1.3388094281676217
1202	21	1.5732577131092071
1202	22	1.3728551124288373
1202	23	1.4441110130036279
1202	24	1.5566697955758073
1202	25	1.873140324252077
1203	1	0.9665684596319615
1203	2	1.0657121744189162
1203	3	0.9827623964329959
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2211	16	-0.15666658680859058
2211	17	0.39805173101018454
2211	18	-0.10600007992697869
2211	19	0.21832996584901565
2211	20	0.036786547347013254
2211	21	0.1917893834960651
2211	22	0.27476372155940254
2211	23	0.4520527467624831
2211	24	0.17380610227201743
2211	25	0.6306034417506083
2212	1	0.11482533056962296
2212	2	0.19253447997351003
2212	3	-0.19832959090669888
2212	4	0.08954627409731586
2212	5	-0.06692110771715676
2212	6	-0.26664581243449703
2212	7	-0.004146828559427718
2212	8	-0.035079798507048565
2212	9	0.06603420591990528
2212	10	-0.27774424842093026
2212	11	-0.31950313402008135
2212	12	0.1642244441084344
2212	13	0.21711767630845458
2212	14	0.2707121736761664
2212	15	-0.11020066448368482
2212	16	-0.03714213695234747
2212	17	0.10689657449816536
2212	18	0.2539084995380634
2212	19	0.011127609875188682
2212	20	0.34644315578111673
2212	21	0.39164221638868923
2212	22	0.4378584816175959
2212	23	0.3579212648085104
2212	24	0.3670980293704602
2212	25	0.586104398852612
2213	1	-0.2988660292706946
2213	2	-0.10476560753311048
2213	3	-0.4666530988669244
2213	4	-0.14954266667844252
2213	5	-0.26914748553727463
2213	6	0.028350742868454404
2213	7	-0.22088433510250383
2213	8	-0.0917058082856812
2213	9	-0.4268703158150515
2213	10	-0.16141978535147672
2213	11	-0.18168131382978459
2213	12	-0.14993192670987554
2213	13	-0.3273354222717799
2213	14	-0.38426578887179375
2213	15	-0.3040942408120557
2213	16	-0.19399919010029265
2213	17	-0.143691599607493
2213	18	-0.359018260645918
2213	19	-0.1586264772473669
2213	20	-0.011011452517735165
2213	21	-0.18928454265690675
2213	22	-0.3013543657968043
2213	23	-0.08173371194450141
2213	24	0.17394355276199036
2213	25	0.3538657911662142
2214	1	-2.7634109434803413
2214	2	-1.3467828709247378
2214	3	-1.4585679259614395
2214	4	-3.4529992182908664
2214	5	-1.7382069465095782
2214	6	-1.414881762279819
2214	7	-1.1537516099265765
2214	8	-1.0145365676475109
2214	9	-0.9041798767247613
2214	10	-0.8294974454043782
2214	11	-0.9699194881610396
2214	12	-0.8732906436657615
2214	13	-1.339932863536184
2214	14	-1.1409029443302927
2214	15	-1.5012287144791117
2214	16	-1.3371192523492255
2214	17	-3.4158908038272138
2214	18	-3.0613767833495125
2214	19	-1.9026406880189697
2214	20	-1.79056060345588
2214	21	-1.814531152300269
2214	22	-2.7833305385444973
2214	23	-1.675380772653007
2214	24	-2.8496827590381493
2214	25	-2.8066739171113824
2215	1	-2.0641841249446173
2215	2	-3.6992564385268594
2215	3	-2.1595168430754583
2215	4	-3.7900525740026225
2215	5	-2.4440043140829673
2215	6	-2.159837727683943
2215	7	-2.3841573956388267
2215	8	-1.6050223491617466
2215	9	-4.135236698791516
2215	10	-2.334811622284299
2215	11	-2.0242118179317217
2215	12	-2.017128581201753
2215	13	-2.2170492089053973
2215	14	-1.904466407574212
2215	15	-2.1090077193389405
2215	16	-2.0877034596011086
2215	17	-2.5052363921445178
2215	18	-2.1115379479717333
2215	19	-2.12153307882852
2215	20	-1.9990504109603648
2215	21	-2.5232221395452115
2215	22	-2.4346776268579475
2215	23	-2.2699534036296285
2215	24	-2.2988704457750586
2215	25	-3.206292637524392
2216	1	-3.146659745125504
2216	2	-3.38539537193963
2216	3	-4.540789599935966
2216	4	-5.170453642368649
2216	5	-4.7847755587713365
2216	6	-3.7845007950689116
2216	7	-5.343187181953969
2216	8	-3.1795011867136402
2216	9	-4.029202341587986
2216	10	-2.830618825482407
2216	11	-3.2670594786769627
2216	12	-3.6178950953760136
2216	13	-3.026273167677683
2216	14	-4.154193043669903
2216	15	-3.648247722244051
2216	16	-3.9296200868144595
2216	17	-3.7051128861111735
2216	18	-3.1760071652580706
2216	19	-3.9968005295901996
2216	20	-4.608476562314294
2216	21	-4.13414675309367
2216	22	-3.7793955495641427
2216	23	-3.9552173861243958
2216	24	-4.341120694631947
2216	25	-4.353816555166841
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108306.0
3	0.0
4	990.0
5	6008.0
6	7358.0
7	6715.0
8	5752.0
9	5483.0
10	4875.0
11	4685.0
12	4518.0
13	4206.0
14	4281.0
15	4310.0
16	4621.0
17	4907.0
18	5546.0
19	6708.0
20	8711.0
21	11115.0
22	14270.0
23	17117.0
24	20220.0
25	22564.0
26	25679.0
27	28733.0
28	32261.0
29	38033.0
30	47098.0
31	57256.0
32	72759.0
33	92574.0
34	120159.0
35	157799.0
36	214165.0
37	327296.0
38	923956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84905198254609	18.28570694447495	12.560469758800622	24.304771314178343
2	15.078523823175372	19.90041283975423	41.86870093035591	23.15236240671449
3	19.516360226535745	25.413229456256374	28.684159434238953	26.386250882968927
4	12.290608720294022	16.160952293219463	37.77104529266963	33.777393693816876
5	13.258750109986984	38.67171250455955	34.57276098516942	13.496776400284045
6	31.987856856746376	35.777256656592854	18.571576793930745	13.66330969273003
7	28.802052287700576	31.598608439148563	21.436915549816177	18.162423723334687
8	25.856230510145267	36.26508435163647	20.516289404547987	17.362395733670283
9	27.968774351115755	14.560752894643612	19.799697152213877	37.67077560202676
10	16.530874803319527	28.500829716672634	33.11065617483664	21.857639305171197
11	34.898010984768	22.15986367841732	24.32097581383274	18.621149522981945
12	24.543774496022756	24.497552464899368	30.84510244918884	20.113570589889036
13	29.587165918648534	21.89057811322502	25.568909232887666	22.953346735238785
14	21.544087614656902	22.327028685102167	27.247911909104587	28.88097179113634
15	23.62835359438514	30.83948840206878	23.15361373190426	22.378544271641818
16	22.31935678701073	27.784385243207783	27.648046842864826	22.248211126916665
17	21.89494356472485	28.378467771943605	27.401662049861496	22.324926613470044
18	21.639365273620324	26.80561297898027	29.88233251194867	21.672689235450743
19	23.84732703238932	26.382064428055173	27.467400035803006	22.303208503752494
20	24.64040495578031	26.532723231708527	27.800897292577574	21.025974519933587
21	24.092536664371053	26.193312736192624	26.571510301739572	23.142640297696754
22	24.142479011087968	27.852387563682008	26.650729454102233	21.35440397112779
23	22.825948996833542	27.464630056916867	27.565878986650773	22.143541959598824
24	23.660234801524997	26.235709377922305	28.171056689544894	21.932999131007808
25	23.159194138558128	26.912015120753953	27.952807174420723	21.975983566267193
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	292.0
1	292.0
2	710.5
3	1129.0
4	1129.0
5	1129.0
6	1906.5
7	2684.0
8	2684.0
9	2684.0
10	3116.5
11	3549.0
12	3549.0
13	3549.0
14	5218.5
15	6888.0
16	6888.0
17	6888.0
18	14076.5
19	21265.0
20	21265.0
21	21265.0
22	35964.5
23	50664.0
24	50664.0
25	50664.0
26	78391.0
27	106118.0
28	106118.0
29	106118.0
30	139406.5
31	172695.0
32	172695.0
33	172695.0
34	196932.5
35	221170.0
36	221170.0
37	221170.0
38	238884.0
39	256598.0
40	256598.0
41	256598.0
42	274618.5
43	292639.0
44	292639.0
45	292639.0
46	312204.5
47	331770.0
48	331770.0
49	331770.0
50	320955.0
51	310140.0
52	310140.0
53	310140.0
54	276177.0
55	242214.0
56	242214.0
57	242214.0
58	208867.0
59	175520.0
60	175520.0
61	175520.0
62	145376.0
63	115232.0
64	115232.0
65	115232.0
66	88493.0
67	61754.0
68	61754.0
69	61754.0
70	45810.5
71	29867.0
72	29867.0
73	29867.0
74	21049.5
75	12232.0
76	12232.0
77	12232.0
78	8405.5
79	4579.0
80	4579.0
81	4579.0
82	3022.0
83	1465.0
84	1465.0
85	1465.0
86	941.5
87	418.0
88	418.0
89	418.0
90	264.5
91	111.0
92	111.0
93	111.0
94	70.5
95	30.0
96	30.0
97	30.0
98	20.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3325438634897321
2	0.00991311976618255
3	0.009252245115103712
4	0.011028345739878086
5	0.012102267047881195
6	0.07810712282438
7	0.07765277150176329
8	0.10193991492891054
9	0.08248541738777729
10	0.08822676591902469
11	0.08459195533809108
12	0.09392680978457965
13	0.08157671474254388
14	0.0858310953088639
15	0.08364194802716525
16	0.08479847866655321
17	0.09640508972612528
18	0.09723118303997383
19	0.09264636514811439
20	0.09095287385472488
21	0.0796353954549998
22	0.09813988568520722
23	0.09264636514811439
24	0.0883919845817944
25	0.08702893061394429
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2421034.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.63808026548106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18564795611337	36.55342635217427
2	14.524200885547451	14.709553004686457
3	5.478764910745601	8.323024119181104
4	2.6703658290602514	5.408887967606034
5	1.4840635472114054	3.757506451138284
6	0.9347110954658147	2.8399185286340187
7	0.6112269265554215	2.166595071713571
8	0.38674650949457573	1.5667280632144767
9	0.3003394109361127	1.3687750078083158
>10	1.3062464694072258	11.083311568114954
>50	0.06437745267940286	2.2538489121650027
>100	0.044692005773462176	4.4476204676274955
>500	0.004924000577117617	1.76683696169413
>1k	0.0033647337276957122	2.7838196064994856
>5k	3.282667051410451E-4	0.9701479177424388
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6982	0.2883891758645273	No Hit
GTCCTACAGTGGACATTTCTAAATT	5901	0.2437388322510134	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5364	0.22155822677417994	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5098	0.21057118569999428	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4070	0.16810998936817906	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3975	0.16418604612739846	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3871	0.15989036089538602	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3229	0.1333727655208477	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2720	0.11234869068340221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.1304665692427285E-5	0.0
11	0.0	0.0	0.0	4.1304665692427285E-5	0.0
12	0.0	0.0	0.0	4.1304665692427285E-5	8.260933138485457E-5
13	0.0	0.0	0.0	4.1304665692427285E-5	1.2391399707728187E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2080	0.0	11.958853	1
TAGGACC	695	0.0	11.889978	4
GTTAGAC	100	1.9349754E-6	11.397978	3
GTATCAA	4560	0.0	11.202431	1
AAATCGC	60	0.0058841193	11.081596	17
GGACGTC	70	0.001494131	10.855217	6
ACACCGT	125	2.2470886E-7	10.638113	6
TAGAAAT	770	0.0	10.608508	4
GGCGAGG	1200	0.0	10.527517	19
GTCGAGG	100	2.4040413E-5	10.448362	19
GTCCTAC	2835	0.0	10.387661	1
TAGACTC	175	2.3646862E-10	10.312457	5
GCGCCAC	250	0.0	10.258181	13
GCGTGCG	260	0.0	10.228532	9
CTTACAC	280	0.0	10.176766	3
AGGACGT	2990	0.0	10.165421	5
TCCTACA	2975	0.0	10.152821	2
GTGTAAG	340	0.0	10.09104	1
CGTGCGC	245	0.0	10.079429	10
TGTAGGA	2980	0.0	10.072039	2
>>END_MODULE