FastQCFastQC Report
Thu 26 May 2016
SRR1512828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421034
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88320.3648028073955178No Hit
TATCAACGCAGAGTACTTTTTTTTT62600.2585672072345948No Hit
GTCCTACAGTGGACATTTCTAAATT53530.22110387545156324No Hit
CTGTAGGACGTGGAATATGGCAAGA51340.21205815366492167No Hit
GGTATCAACGCAGAGTACTTTTTTT49250.2034254785352044No Hit
GTCCTAAAGTGTGTATTTCTCATTT48410.19995588661704047No Hit
CTTTAGGACGTGAAATATGGCGAGG43270.17872528845113286No Hit
GTACTGGTTCACTATCGGTCAGTCA30170.12461617639405312No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29770.12296398976635603No Hit
GTCCTACAGTGTGCATTTCTCATTT25860.10681386548061696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1900.014.48746918
GGTATCA20850.012.9132121
GCCGGTT1900.012.4899711
CCAACGT1155.3987605E-912.38059619
TAGGACC6700.011.9240444
GGCGAGG11050.011.76810219
CGGTTTC1701.8189894E-1211.72515313
CCGGTTT2050.010.64932512
CCAACGA1252.2684617E-710.63080519
TTAGGAC16100.010.57613853
TAGGACA8200.010.5547184
GTATCAA44950.010.4133231
GTCCTAC27350.010.4008251
TAGAAAT6900.010.3378744
CACCTTT8550.010.21381114
TGGACCG750.002623532710.1449015
GACGTGG12300.010.1241987
AGGACGT26450.010.0681925
TCGCTCG856.6068606E-410.0569859
GTCCTAT2750.010.0327321
Produced by FastQC (version 0.11.5)